CN204752682U - Water microbial ecology normal position experimental apparatus - Google Patents
Water microbial ecology normal position experimental apparatus Download PDFInfo
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- CN204752682U CN204752682U CN201520342385.XU CN201520342385U CN204752682U CN 204752682 U CN204752682 U CN 204752682U CN 201520342385 U CN201520342385 U CN 201520342385U CN 204752682 U CN204752682 U CN 204752682U
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Abstract
The utility model discloses a water microbial ecology normal position experimental apparatus, characteristics are including being used for cultivateing the experiment bottle of microorganism and being used for placing the superficial frame of open -type that the bottle was tested to a plurality of, the bottleneck of testing the bottle seals up to be provided with and is used for realization bottle interior microorganism and the mutual isolation of bottle outer environmental facies just bottle interior water and the mutual filter membrane that circulates of environment water, the bottom of floating the frame with and both sides be provided with a plurality of trompil respectively, the diameter of trompil is less than the bottleneck diameter of testing the bottle, the experiment bottle is arranged in and is floated the interior position of just testing the bottleneck alignment trompil of bottle of frame, the filtration pore aperture of filter membrane is 0.2 mu m -10 mu m, the bottleneck of experiment bottle is provided with the perforation bottle lid, the filter membrane covers the inboard that just is located the perforation bottle lid at the bottleneck of experiment bottle, the advantage can realize microorganism and experiment water mutual isolation, it keeps unanimous nevertheless to test the interior quality of water with the experiment water of container, can carry out simultaneously the little biological assay of multiunit cultivates, the experimental efficiency is improved.
Description
Technical field
The utility model relates to a kind of Water microbial ecology experimental installation.
Background technology
China's eutrophication of natural water is serious, and water ecosystem is overbalance.As the microorganism of decomposer in aquatic ecosystem, extremely important effect is played for self purification of water body and reparation, thus understand the composition of microflora and its dynamic change and and water surrounding between mutual relationship be very important to carrying out water environment biological repairing research.
The Water microbial ecology research carried out at present, its sample is mainly derived from natural water water sample, the cultivation water sample of laboratory simulation field environment and field enclosure ecosystem water sample; The natural water water sample taked can only characterize microorganisms in water structure of community composition at that time, and disclose biological community structure succession course and rule needs to accumulate data for many years; Laboratory culture water sample, is difficult to simulate the environment consistent with field completely, as the factors such as temperature variation, illumination, wind, tidal action are difficult to simulation, causes experimental studies results to be difficult to be advantageously used in the microbial ecological During Succession inferred in the physical environment of field; Current microbial ecological encloses lattice experiment, and the lattice that enclose of use are fluid-tight substantially, enclose inside and outside lattice and do not have exchange of substance, and enclosing physical and chemical factor inside and outside lattice in the whole experimental phase can not synchronously change.Water microbial ecology research needs a kind of original position experimental installation and method, while the change of experimental installation internal and external environment factor synchronization can be realized, microorganism inside and outside experimental installation can be mutually isolated, for disclosing microorganisms in water structure of community to the response characteristic of some pollution type water body and succession course thereof, to disclose the multifarious ecosystem function maintenance mechanism of microorganisms in water from regional scale.The enclosure device of existing microbial ecological original position experiment is water tight apparatus substantially, and the physical environment that only can realize Culture in situ is consistent, but the chemokines large to microorganism growth influence then cannot be consistent with outside atmosphere.
Summary of the invention
Technical problem to be solved in the utility model be to provide one can realize microorganism with experiment water body mutually isolated, but be consistent with the water quality of experiment water body in experiment container, the Experiment on Microbiology of many groups can be carried out cultivate simultaneously, improve the Water microbial ecology original position experimental installation of conventional efficient.
The utility model solves the problems of the technologies described above adopted technical scheme: a kind of Water microbial ecology original position experimental installation, it is characterized in that comprising and float frame for the experiment bottle of culturing micro-organisms and the open type for placing the experiment bottle described in several, the bottle sealing of described experiment bottle is provided with the filter membrane mutually circulated for realizing microorganism and bottle external environment in bottle mutually isolated and water in bottle sample and environment water, bottom and its both sides of described floating frame are respectively arranged with several perforates, the diameter of described perforate is less than the bottleneck diameter of described experiment bottle, described experiment bottle inversion or horizontal in described floating frame.
The filter opening aperture of described filter membrane is 0.2 μm-10 μm.
The bottleneck of described experiment bottle is provided with perforation bottle cap, and described filter membrane covers the bottleneck of described experiment bottle and is positioned at the inner side of described perforation bottle cap.Can be used for fixing and protect filter membrane not to be destroyed.
Sealing-ring is provided with between the bottleneck of described filter membrane and described experiment bottle.
The arranged outside of described filter membrane has plastic silk screen.Further protection filter membrane is not destroyed, and ensures the circulation of water.
The both sides, top of described floating frame are provided with floating drum.Frame is floated in further guarantee can float on experiment water surface.
The bottom of described floating frame and its both sides are respectively arranged with the groove for fixing described experiment bottle position of bottleneck, and described perforate is arranged on the cell wall of described groove.Groove being set for placing experiment bottle, making it motionless at floating frame internal fixtion.
Described experiment bottle is Plastic Bottle or vial, and described floating frame is sponge plastics basket.
Compared with prior art, the utility model has the advantage of: a kind of Water microbial ecology original position of the utility model experimental installation, utilize experiment bottle bottleneck filter membrane by the microorganism in experiment container and bottle external environment mutually isolated, but water sample and bottle are tested the water body water quality that mutually circulates outward and can be consistent that (this filter membrane allows various resolvable chemical material to pass through in experiment container, thus the physical and chemical factor factor of water in bottle sample and bottle external environment water body is consistent), thus realize the Culture in situ experiment of microorganisms in water, for studying the responsive status of microorganisms in water structure of community to different water environment.Float frame by open type to arrange, many group microorganisms can be realized and cultivate simultaneously, improve conventional efficient.
Accompanying drawing explanation
Fig. 1 is the utility model Water microbial ecology original position experimental installation structural representation one;
Fig. 2 is the utility model Water microbial ecology original position experimental installation structural representation two;
Fig. 3 is the structural representation that the utility model floats frame.
Embodiment
Below in conjunction with accompanying drawing embodiment, the utility model is described in further detail.
Specific embodiment
A kind of Water microbial ecology original position experimental installation, as depicted in figs. 1 and 2, comprise and float frame 2 for the experiment bottle 1 of culturing micro-organisms and the open type for placing several experiment bottles 1, the bottle sealing of experiment bottle 1 is provided with the filter membrane 3 mutually circulated for realizing microorganism and bottle external environment in bottle mutually isolated and water in bottle sample and environment water, bottom and its both sides of floating frame 2 are respectively arranged with several perforates 4, the diameter of perforate 4 is less than the bottleneck diameter of experiment bottle 1, and experiment bottle 1 is inverted or horizontal in floating frame 2.
In this particular embodiment, the filter opening aperture of filter membrane 3 is 0.2 μm-10 μm, experiment bottle 1 bottleneck be provided with perforation bottle cap 5, filter membrane 3 cover experiment bottle 1 bottleneck and be positioned at perforation bottle cap 5 inner side.Sealing-ring 6 is provided with between the bottleneck of filter membrane 3 and experiment bottle 1.The outer side seal of filter membrane 3 is provided with and is not destroyed for the protection of filter membrane 3 and ensures the plastic silk screen 7 of the current general character.
In this particular embodiment, as shown in Figure 3, the both sides, top of floating frame 2 are provided with floating drum 8.The bottom of floating frame 2 and its both sides are respectively arranged with the groove 9 for fixing experiment bottle 1 position of bottleneck, and perforate 4 is arranged on the cell wall of groove 9.Experiment bottle 1 is Plastic Bottle or vial, and floating frame 2 is sponge plastics basket.
Application Example
Study certain waters microorganism to study the succession response process of different water environment.Adopt experiment bottle 1(Plastic Bottle or the vial of multiple volume about 1.0L), bottleneck bore and common filter membrane 3 diameter supporting, the in-built experiment water of bottle after sterilizing, bottom perforation bottle cap 5 and bottleneck pad the gasket (not shown) after sterilizing respectively, 0.2 μm of filter membrane 3 is placed in rear flank in perforation bottle cap 5 experiment bottle 1 is sealed, then horizontal for multiple experiment bottle 1 (also can be inverted) entirety after sponge plastics floats basket 2 and tests the position of the bottleneck alignment apertures 4 of bottle 1 is immersed in experiment waters.Because 0.2 μm of filter membrane 3 can the microorganism of isolation bottle inside and outside, but can, through mineral ion and dissolved organic matter, physical and chemical factor inside and outside bottle can be consistent substantially, realize the original position experiment of microorganism in experiment bottle 1; In test, directly experiment bottle 1 is fetched process can obtain microbiological specimens.
Above-mentioned filter membrane 3 type can according to the primary vegeto-animal size of certain class of research, be not limited to 0.2 μm of filter membrane 3, the filter membrane 3 of different pore size can be changed to, also can use the double-layered filtration film 3 of two kinds of different pore sizes simultaneously, can be used in the biological community structure succession studied after the microorganism of a certain particle size range of isolation.
Above-described embodiment further illustrates of the present utility model, but protection domain of the present utility model is as the criterion with claims.
Claims (8)
1. a Water microbial ecology original position experimental installation, it is characterized in that comprising and float frame for the experiment bottle of culturing micro-organisms and the open type for placing the experiment bottle described in several, the bottle sealing of described experiment bottle is provided with the filter membrane mutually circulated for realizing microorganism and bottle external environment in bottle mutually isolated and water in bottle sample and environment water, bottom and its both sides of described floating frame are respectively arranged with several perforates, the diameter of described perforate is less than the bottleneck diameter of described experiment bottle, described experiment bottle inversion or horizontal in described floating frame.
2. a kind of Water microbial ecology original position experimental installation according to claim 1, is characterized in that: the filter opening aperture of described filter membrane is 0.2 μm-10 μm.
3. a kind of Water microbial ecology original position experimental installation according to claim 2, it is characterized in that: the bottleneck of described experiment bottle is provided with perforation bottle cap, described filter membrane covers the bottleneck of described experiment bottle and is positioned at the inner side of described perforation bottle cap.
4. a kind of Water microbial ecology original position experimental installation according to claim 3, is characterized in that: be provided with sealing-ring between the bottleneck of described filter membrane and described experiment bottle.
5. a kind of Water microbial ecology original position experimental installation according to claim 3, is characterized in that: the arranged outside of described filter membrane has plastic silk screen.
6. a kind of Water microbial ecology original position experimental installation according to claim 1, is characterized in that: the both sides, top of described floating frame are provided with floating drum.
7. a kind of Water microbial ecology original position experimental installation according to claim 1, it is characterized in that: the bottom of described floating frame and its both sides are respectively arranged with the groove for fixing described experiment bottle position of bottleneck, and described perforate is arranged on the cell wall of described groove.
8. a kind of Water microbial ecology original position experimental installation according to any one of claim 1-7, it is characterized in that: described experiment bottle is Plastic Bottle or vial, described floating frame is sponge plastics basket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520342385.XU CN204752682U (en) | 2015-05-25 | 2015-05-25 | Water microbial ecology normal position experimental apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520342385.XU CN204752682U (en) | 2015-05-25 | 2015-05-25 | Water microbial ecology normal position experimental apparatus |
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| CN204752682U true CN204752682U (en) | 2015-11-11 |
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| CN201520342385.XU Expired - Fee Related CN204752682U (en) | 2015-05-25 | 2015-05-25 | Water microbial ecology normal position experimental apparatus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107703261A (en) * | 2017-09-26 | 2018-02-16 | 中国环境科学研究院 | A kind of microorganism and the experimental simulation device and experimental method of deposit interaction |
| CN109410704A (en) * | 2018-12-06 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | Large spatial scale field portable microcosm experiment device and method |
| CN109596792A (en) * | 2018-11-12 | 2019-04-09 | 中国地质科学院岩溶地质研究所 | A method of utilizing RDOC content in culture of microorganism detection water sample in situ |
| CN112029632A (en) * | 2020-09-04 | 2020-12-04 | 北京博瑞兴环境科技有限公司 | In-situ culture and enrichment device for anaerobic microorganisms in underground water and using method |
| JP2021503913A (en) * | 2017-11-29 | 2021-02-15 | コーニング インコーポレイテッド | Cap for cell culture equipped with a filter and cell culture method |
-
2015
- 2015-05-25 CN CN201520342385.XU patent/CN204752682U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107703261A (en) * | 2017-09-26 | 2018-02-16 | 中国环境科学研究院 | A kind of microorganism and the experimental simulation device and experimental method of deposit interaction |
| JP2021503913A (en) * | 2017-11-29 | 2021-02-15 | コーニング インコーポレイテッド | Cap for cell culture equipped with a filter and cell culture method |
| CN109596792A (en) * | 2018-11-12 | 2019-04-09 | 中国地质科学院岩溶地质研究所 | A method of utilizing RDOC content in culture of microorganism detection water sample in situ |
| CN109410704A (en) * | 2018-12-06 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | Large spatial scale field portable microcosm experiment device and method |
| CN112029632A (en) * | 2020-09-04 | 2020-12-04 | 北京博瑞兴环境科技有限公司 | In-situ culture and enrichment device for anaerobic microorganisms in underground water and using method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151111 Termination date: 20180525 |