CN204676024U - From moving liquid type constant temperature dynamic dialysis system - Google Patents
From moving liquid type constant temperature dynamic dialysis system Download PDFInfo
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- CN204676024U CN204676024U CN201520335228.6U CN201520335228U CN204676024U CN 204676024 U CN204676024 U CN 204676024U CN 201520335228 U CN201520335228 U CN 201520335228U CN 204676024 U CN204676024 U CN 204676024U
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Abstract
The utility model discloses a kind of from moving liquid type constant temperature dynamic dialysis system, comprise the thermostat container that bottom is provided with magnetic agitation controller and intelligent temperature controller, the liquid-inlet pipe of described thermostat container is communicated with damping fluid bucket by fluid filling pump, and the liquid discharge pipe of thermostat container is communicated with waste liquid barrel by draw-off pump; Described magnetic agitation controller, intelligent temperature controller, fluid filling pump are connected with singlechip controller sampled signal input terminus with the signal output part of draw-off pump, and the output control terminal of described singlechip controller is connected with the electric machine controller input control end of fluid filling pump, draw-off pump and the input control end of magnetic agitation controller respectively.What the utility model provided moves liquid type constant temperature dynamic dialysis system certainly, and level of automation is high, safe and practical, convenient and swift, effectively can save manpower, has higher repeatability and controllability.
Description
Technical field
The utility model relates to biological technical field dialysis equipment, especially relates to a kind of from moving liquid type constant temperature dynamic dialysis system.
Background technology
In laboratory separation and purification protein processes, the normal small-molecule substance such as salt, foreign protein utilized in the method removing protein soln of dialysis, when inside and outside dialysis tubing, solution reaches equilibrium state, needs to change external buffer liquid.In actually operating, be generally that the damping fluid containing salt, foreign protein etc. is once toppled over by timing, be then replaced by new damping fluid.Especially for antibody purification mark, the mode of exchange buffering liquid at regular time and quantity need be adopted.Because dialysis is successive processes, the time length is long again, when needs are when carrying out buffer exchange night, can affect the normal rest of operator.And some have the project of particular requirement to temperature, during exchange buffering liquid, easily cause the fluctuation of temperature, usually occur that testing situation about can not carry out smoothly occurs.
Summary of the invention
The utility model provides a kind of from moving liquid type constant temperature dynamic dialysis system, and object is to solve in dialysis procedure the problem of manually changing liquid and wasting time and energy.
For achieving the above object, the utility model can take following technical proposals:
Described in the utility model from moving liquid type constant temperature dynamic dialysis system, comprise the thermostat container that bottom is provided with magnetic agitation controller and intelligent temperature controller, the liquid-inlet pipe of described thermostat container is communicated with damping fluid bucket by fluid filling pump, and the liquid discharge pipe of thermostat container is communicated with waste liquid barrel by draw-off pump; Described magnetic agitation controller, intelligent temperature controller, fluid filling pump are connected with singlechip controller sampled signal input terminus with the signal output part of draw-off pump, and the output control terminal of described singlechip controller is connected with the electric machine controller input control end of fluid filling pump, draw-off pump and the input control end of magnetic agitation controller respectively.
The rack for hanging dialysis tubing is movably set with in described thermostat container.
Described rack is fixed with clip.
Described liquid-inlet pipe and liquid discharge pipe are provided with check valve.
The liquid feeding end of described liquid-inlet pipe is provided with strainer.
Described thermostat container top is provided with seal cover board.
What the utility model provided moves liquid type constant temperature dynamic dialysis system certainly, and level of automation is high, safe and practical, convenient and swift, effectively can save manpower, has higher repeatability and controllability.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation that rack of the present utility model hangs dialysis tubing.
Embodiment
Described in the utility model from moving liquid type constant temperature dynamic dialysis system, comprise the thermostat container 3 that bottom is provided with digital display temperature constant magnetic stirring controller 1 and intelligent temperature controller 2, described thermostat container 3 top has fluid inlet, fluid inlet connects liquid-inlet pipe 4, liquid-inlet pipe 4 is communicated with damping fluid bucket 6 by constant current fluid filling pump 5, the bottom of thermostat container 3 has leakage fluid dram, leakage fluid dram connected drainage pipe 7, and liquid discharge pipe 7 is communicated with waste liquid barrel 9 by constant current draw-off pump 8; Thermostat container 3 is equipped with singlechip controller 10, described magnetic agitation controller 1, intelligent temperature controller 2, constant current fluid filling pump 5 are connected with singlechip controller 10 sampled signal input terminus with the signal output part of constant current draw-off pump 8, and the output control terminal of described singlechip controller 10 is connected with the electric machine controller input control end of fluid filling pump 5, draw-off pump 8 and the input control end of magnetic agitation controller 1 respectively.Being connected with detachable rack 12 in thermostat container 3, detachable rack 12 being fixed with the clip 13 for hanging dialysis tubing 11.The liquid feeding end of liquid-inlet pipe 4 is provided with microfilter, the damping fluid entering thermostat container 3 can be filtered, liquid-inlet pipe 4 and liquid discharge pipe 7 are provided with check valve 14, prevent the liquid generation adverse current in pipeline, cause polluting fresh buffer containing assorted damping fluid, and then affect protein purification result.Thermostat container 3 top is provided with insulation, dust-proof seal cover board 15.
During use, open the seal cover board 15 on thermostat container 3 top, take out detachable rack 12, in thermostat container 3, add appropriate amount of buffer solution, put into stirrer.Fixedly fill the dialysis tubing 11 of protein example with clip 13, then detachable rack 12 is snapped fit onto in thermostat container 3, dialysis tubing 11 is hung in damping fluid.Connect the power supply of thermostat container 3, set dialysis time according to requirement of experiment, change liquid measure, change the parameter such as liquid number of times, damping fluid temperature, start stirring, thermostat.After dialysis certain hour, singlechip controller 10 receives the time signal that magnetic stirring apparatus 1 sends, sending instruction makes constant current fluid filling pump 5 and constant current draw-off pump 8 start working, with in the fresh buffer displacement thermostat container 3 in damping fluid bucket 6 containing assorted waste liquid, enter in waste liquid barrel 9 containing assorted waste liquid.According to the temperature signal that intelligent temperature controller 2 sends, singlechip controller 10 controls the working flow of constant current fluid filling pump 5 and/or constant current draw-off pump 8.According to the flow signal that constant current fluid filling pump 5 and constant current draw-off pump 8 send, singlechip controller 10 makes constant current fluid filling pump 5 and constant current draw-off pump 8 quit work, and buffer exchange completes.According to the time signal that magnetic stirring apparatus 1 sends, repeat above replacement process.
Claims (6)
1. certainly move liquid type constant temperature dynamic dialysis system for one kind, it is characterized in that: comprise the thermostat container (3) that bottom is provided with magnetic agitation controller (1) and intelligent temperature controller (2), the liquid-inlet pipe (4) of described thermostat container (3) is communicated with damping fluid bucket (6) by fluid filling pump (5), and the liquid discharge pipe (7) of thermostat container (3) is communicated with waste liquid barrel (9) by draw-off pump (8); Described magnetic agitation controller (1), intelligent temperature controller (2), fluid filling pump (5) are connected with singlechip controller (10) sampled signal input terminus with the signal output part of draw-off pump (8), and the output control terminal of described singlechip controller (10) is connected with the electric machine controller input control end of fluid filling pump (5), draw-off pump (8) and the input control end of magnetic agitation controller (1) respectively.
2. according to claim 1 from moving liquid type constant temperature dynamic dialysis system, it is characterized in that: in described thermostat container (3), be movably set with the rack (12) for hanging dialysis tubing (11).
3. according to claim 2 from moving liquid type constant temperature dynamic dialysis system, it is characterized in that: described rack (12) is fixed with clip (13).
4. according to claim 1 from moving liquid type constant temperature dynamic dialysis system, it is characterized in that: described liquid-inlet pipe (4) and liquid discharge pipe (7) are provided with check valve (14).
5. according to claim 1 from moving liquid type constant temperature dynamic dialysis system, it is characterized in that: the liquid feeding end of described liquid-inlet pipe (4) is provided with strainer.
6. according to claim 1 from moving liquid type constant temperature dynamic dialysis system, it is characterized in that: described thermostat container (3) top is provided with seal cover board (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520335228.6U CN204676024U (en) | 2015-05-22 | 2015-05-22 | From moving liquid type constant temperature dynamic dialysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520335228.6U CN204676024U (en) | 2015-05-22 | 2015-05-22 | From moving liquid type constant temperature dynamic dialysis system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204676024U true CN204676024U (en) | 2015-09-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520335228.6U Active CN204676024U (en) | 2015-05-22 | 2015-05-22 | From moving liquid type constant temperature dynamic dialysis system |
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| Country | Link |
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| CN (1) | CN204676024U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108264536A (en) * | 2018-03-27 | 2018-07-10 | 润辉生物技术（威海）有限公司 | A kind of continuous high-flux polypeptide synthesizer and its application method |
-
2015
- 2015-05-22 CN CN201520335228.6U patent/CN204676024U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108264536A (en) * | 2018-03-27 | 2018-07-10 | 润辉生物技术（威海）有限公司 | A kind of continuous high-flux polypeptide synthesizer and its application method |
| CN108264536B (en) * | 2018-03-27 | 2023-08-08 | 润辉生物技术（威海）有限公司 | Continuous high-flux polypeptide synthesis device and application method thereof |
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