CN204625631U - A kind of immuno magnetic cell separation acid-fast bacilli detection kit - Google Patents
A kind of immuno magnetic cell separation acid-fast bacilli detection kit Download PDFInfo
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- CN204625631U CN204625631U CN201520050027.1U CN201520050027U CN204625631U CN 204625631 U CN204625631 U CN 204625631U CN 201520050027 U CN201520050027 U CN 201520050027U CN 204625631 U CN204625631 U CN 204625631U
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- fast bacilli
- cell separation
- detection kit
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Abstract
The utility model relates to a kind of immuno magnetic cell separation acid-fast bacilli detection kit, comprise box body and lid, described lid is connected to above box body, lid and the box body place of covering are provided with gasket, bottle holder is provided with in described box body, described bottle holder is provided with groove, comprises the groove placing slide glass, the groove placing cover glass, places the groove of magnet, the groove of placing glass pipe and place the groove of reagent bottle, and has one corresponding to indicate the label placed name and claim below each groove.Separation acid-fast bacilli has simply, feature fast and accurately to adopt test kit of the present utility model to carry out, and without the need to the specific installation of costliness, just can promote the use of at basic hospital.
Description
Technical field
The utility model relates to a kind of mushroom detection kit, specifically, relates to a kind of immuno magnetic cell separation acid-fast bacilli detection kit.
Background technology
Current China in Tubercufosis control first problems faced be diagnosis lungy.Phthisical laboratory diagnostic method mainly contains bacteriology checking, immunologic test and Bio-molecular analysis.Bacteriology checking is the gold standard of current infectivity diagnosis of pulmonary tuberculosis, mainly contains Sputum smears acid-fast stain and directly looks for Mycobacterium tuberculosis and Mycobacterium tuberculosis cultural method.Traditional smear acid-fast stain is comparatively simple but Sputum smears positive rate is lower, and microscopy needs experience, is difficult to distinguish the deficiencies such as the false positive that environment mycobacterium causes.
The amynologic diagnostic method carried out at present, as T-SPOT, its principle is for detecting tubercle bacillus specific cell immune response function, the gamma-interferon discharged after complete blood cell is stimulated by Specific Antigen of Mycobacterium Tuberculosis is detected by Quantitative in vitro, judge whether it has for the specific cell immune response of mycobacterium tuberculosis, can be used for auxiliary diagnosis lungy, but by immune status, and the factor impact such as expense is high, not easily at Grass-roots Hospital.The Bio-molecular analysis method of rising in recent years, as PCR method, gene chip etc., substantially achieves object that is quick, sensitive, diagnosis of tuberculosis exactly, but there is poor specificity, easily the problem such as pollution and stdn.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the utility model provides a kind of immuno magnetic cell separation acid-fast bacilli detection kit, overcome the false-positive deficiency being difficult to differentiation mycobacterium and causing, and there is special, sensitive, free of contamination feature, without the need to the specific installation of costliness, can promote the use of at basic hospital.
To achieve these goals, the utility model provides a kind of immuno magnetic cell separation acid-fast bacilli detection kit, comprise box body and lid, described lid is connected to above box body, lid and the box body place of covering are provided with gasket, gasket can adopt sponge pad, bottle holder is provided with in described box body, described bottle holder is provided with groove, comprise the groove placing slide glass, the groove placing cover glass, place the groove of magnet, the groove of placing glass pipe and place the groove of reagent bottle, and below each groove, have one corresponding to indicate the label placed name and claim.
The groove of described placement reagent bottle is 6, and correspondingly in each reagent bottle be contained with PBS damping fluid, PE marks anti-acid-fast bacilli antibody, anti-igg Dynabeads immunomagnetic beads, 5% PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine solution, 3% hydrochloride alcohol solution, PBS damping fluid is housed in reagent bottle described in methylene blue solution, PE marks anti-acid-fast bacilli antibody, anti-igg Dynabeads immunomagnetic beads, 5% PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine solution, 3% hydrochloride alcohol solution, methylene blue solution.
In order to test smoothly, and it is easy to use to consider that test kit controls in more suitable size, wherein place the groove of slide glass, place the groove of cover glass, the groove volume of placing glass test tube and be 50, wherein 5% PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine solution, 3% hydrochloride alcohol solution, each 100ml of methylene blue solution, PBS damping fluid 500ml, it is 50 μ l that PE marks anti-acid-fast bacilli antibody, and anti-igg Dynabeads immunomagnetic beads is 250 μ l.
As further improvement, snubber assembly is provided with in described groove, placement thing is fixed in groove preferably, not easily rock in transportation, make test kit can not cause in groove the generation of placing thing cracky situation in moving process, described snubber assembly can be sponge pad or other flexible materials, and has certain heat insulation effect.
As further improvement, in described box body, be also provided with a temperature regulating device, test kit can be made in transportation, or take out reagent and do in experimentation and keep the reagent in test kit substantially to maintain temperature required for experiment.
Preferably, the groove number on bottle holder is at least 10.
Can preserve preferably and use in order to ensure the reagent in test kit, test kit storage temperature is preferably 4 DEG C, and the shelf time is no more than 6 months.
Beneficial effect: test kit of the present utility model provides required reagent and experiment equipment for immuno magnetic cell separation acid-fast bacilli; can directly carry out testing and need not take time and effort for preparing experiment condition again, can also in transportation, play better protecting effect to article in test kit and provide a temperature required condition of experimentation to reagent.And it is positive or negative to detect acid-fast stain display accurately, and then the negative phthisical diagnosis efficiency of phlegm bacterium can be significantly improved.The utility model utilizes the advantage such as quick, simple of immunoreactive high degree of specificity and magnetic resolution, make to adopt the test kit of utility model to carry out separation acid-fast bacilli and there is simple, quick, accurate, free of contamination feature, without the need to the specific installation of costliness, just can promote the use of at basic hospital.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model embodiment.
1 box body in figure, 2 lids, 3 bottle holder, the groove of 4 placement slide glasss, the groove of 5 placement cover glasses, the groove of 6 placement magnet, the groove of 7 placing glass pipes, 8,9,10,11,12,13 are the groove placing reagent bottle, 14 temperature regulating devices.
Embodiment
Below in conjunction with specific embodiment, the utility model is described further.
Embodiment 1
Immuno magnetic cell separation acid-fast bacilli detection kit, comprise box body 1 and lid 2, described lid 2 is connected to above box body 1, lid 2 and box body 1 place of covering are provided with gasket, gasket can adopt sponge pad, bottle holder 3 is provided with in described box body, described bottle holder is provided with groove, comprise the groove 4 placing slide glass, the groove 5 placing cover glass, place the groove 6 of magnet, the groove 7 of placing glass pipe and place the groove 8,9,10,11,12,13 of reagent bottle, and below each groove, have one corresponding to indicate the label placed name and claim.
Be equipped with in the reagent bottle of its further groove 8 in the reagent bottle of PBS damping fluid 500ml, groove 9 and PE is housed marks in the reagent bottle of anti-acid-fast bacilli antibody 50 μ l, groove 10 in the reagent bottle being equipped with in the reagent bottle of anti-igg Dynabeads immunomagnetic beads 250 μ l, groove 11 and 5% PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine solution 100ml, groove 12 are housed to be equipped with in the reagent bottle of 3% hydrochloride alcohol solution 100ml, groove 13 methylene blue solution 100ml is housed.
Sponge pad is provided with in the groove of test kit, placement thing is fixed in groove preferably, not easily rock in transportation, make test kit can not cause in groove the generation of placing thing cracky situation in moving process, and there is certain heat insulation effect.
In addition, between bottle holder and lid, also can adding last layer sponge pad, also can play to placing in bottle holder thing the effect cushioning and be incubated.
Also be provided with a temperature regulating device 14 in box body, test kit can be made in transportation, or take out reagent and do in experimentation and keep the reagent in test kit substantially to maintain temperature required for experiment.
Test kit storage temperature is preferably 4 DEG C, and the shelf time is no more than 6 months.
Certainly, the groove number in this test kit also can more than 10, in order to placing reagent needed for experiment and equipment in an experiment.
Use 19 infiltrative pulmonary tuberculosis patients as volunteer below, get their dark expectoration as sample.With the reagent in test kit and equipment, following steps are adopted to carry out separation acid-fast bacilli:
Step one: get dark expectoration sample, add PE and mark anti-acid-fast bacilli antibody 1 μ l, hatch 30 minutes for 4 DEG C, with PBS damping fluid wash the cell after hatching once after, add 50 μ l anti-igg Dynabeads immunomagnetic beadses, mixing is placed on 4 DEG C and hatches 20 minutes, then separator column is placed in, and separator column being fit in magnetic field 2 minutes, abandoning supernatant after taking out, with 2ml PBS/1 × 10
7individual Dynabeads washes 4 times, puts into whizzer under rotating speed is 3000 revs/min centrifugal 10 minutes;
Step 2: get centrifugal sediment 0.1ml, puts into slide glass centre, and uniform application becomes the oval phlegm film of 2cm × 2.5cm, seasoning, smear flame is fixed, and keeps flat on staining rack, adds the full phlegm film of 5% PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine lid, and low baking temperature is heated to dye liquor and presented steam, reduce internal heat flame, dyes 7 minutes, washing, add the full phlegm film of 3% hydrochloride alcohol lid, decolour 3 minutes, to redfree, washing, add the full phlegm film of methylene blue lid, direct smear redyes 30 seconds, collection bacterium smear redyes 2 minutes, washing, then seasoning;
Step 3: observe under smear dried in step 2 being placed in microscope (eyepiece 10 ×, oily mirror 100 ×), by observing smear acid-fast stain display positive or negative.
Result shows, and in 19 routine infiltrative pulmonary tuberculosis patients, 7 routine Sputum smears acid-fast stains check positive, and after immunological magnetic bead sorting, acid-fast stain is still positive; In 12 routine sputum smear negative lungers, after immunological magnetic bead sorting, 6 routine acid-fast stains are positive, and 6 routine common bacteria patients with pneumonia acid-fast stains after immunological magnetic bead sorting are feminine gender.As can be seen from the results, acid-fast stain display is positive or negative to adopt test kit of the present utility model to detect accurately, and has simple to operate, feature fast, also can use at basic hospital.
The utility model is not limited to above-described embodiment; do not departing under the utility model spirit and real situation thereof; those of ordinary skill in the art can make various corresponding change and distortion according to the utility model; these are tackled amendment that the utility model carries out mutually or equivalently to replace, and it all should be encompassed in the middle of the scope of claim of the present utility model protection.
Claims (8)
1. an immuno magnetic cell separation acid-fast bacilli detection kit, comprise box body and lid, described lid is connected to above box body, it is characterized in that: lid and the box body place of covering are provided with gasket, bottle holder is provided with in described box body, described bottle holder is provided with groove, comprises the groove placing slide glass, the groove placing cover glass, places the groove of magnet, the groove of placing glass pipe and place the groove of reagent bottle, and has one corresponding to indicate the label placed name and claim below each groove.
2. immuno magnetic cell separation acid-fast bacilli detection kit according to claim 1, it is characterized in that: the groove of described placement reagent bottle is 6, and in each reagent bottle, correspondence is contained with PBS damping fluid, PE marks anti-acid-fast bacilli antibody, anti-igg Dynabeads immunomagnetic beads, PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine solution, hydrochloride alcohol solution, methylene blue solution.
3. immuno magnetic cell separation acid-fast bacilli detection kit according to claim 1, is characterized in that: described gasket is sponge pad.
4. immuno magnetic cell separation acid-fast bacilli detection kit according to claim 1, is characterized in that: be provided with snubber assembly in described groove.
5. immuno magnetic cell separation acid-fast bacilli detection kit according to claim 1, is characterized in that: the groove of described placement slide glass, the placement groove of cover glass, the groove volume of placing glass test tube are 50.
6. immuno magnetic cell separation acid-fast bacilli detection kit according to claim 1, is characterized in that: be provided with a temperature regulating device in described box body.
7. the immuno magnetic cell separation acid-fast bacilli detection kit according to any one of claim 1 to 6, is characterized in that: described groove is at least 10.
8. the immuno magnetic cell separation acid-fast bacilli detection kit according to any one of claim 1 to 6, is characterized in that: described test kit storage temperature is 4 DEG C, and the shelf time is no more than 6 months.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520050027.1U CN204625631U (en) | 2015-01-23 | 2015-01-23 | A kind of immuno magnetic cell separation acid-fast bacilli detection kit |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520050027.1U CN204625631U (en) | 2015-01-23 | 2015-01-23 | A kind of immuno magnetic cell separation acid-fast bacilli detection kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560696A (en) * | 2015-01-23 | 2015-04-29 | 皖南医学院弋矶山医院 | Detection kit for separating acid-fast bacillus by immunomagnetic beads |
| CN106884013A (en) * | 2017-04-21 | 2017-06-23 | 皖南医学院 | A kind of primer pair and its detection method and kit for detecting ApoM promoter gene sequences |
-
2015
- 2015-01-23 CN CN201520050027.1U patent/CN204625631U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560696A (en) * | 2015-01-23 | 2015-04-29 | 皖南医学院弋矶山医院 | Detection kit for separating acid-fast bacillus by immunomagnetic beads |
| CN106884013A (en) * | 2017-04-21 | 2017-06-23 | 皖南医学院 | A kind of primer pair and its detection method and kit for detecting ApoM promoter gene sequences |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150909 Termination date: 20170123 |