CN204625613U - A kind of detachable fluorescent quantitation orifice plate - Google Patents
A kind of detachable fluorescent quantitation orifice plate Download PDFInfo
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- CN204625613U CN204625613U CN201520308918.2U CN201520308918U CN204625613U CN 204625613 U CN204625613 U CN 204625613U CN 201520308918 U CN201520308918 U CN 201520308918U CN 204625613 U CN204625613 U CN 204625613U
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- plate
- sptting plate
- sptting
- fluorescent quantitation
- orifice
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Abstract
The utility model discloses a kind of detachable fluorescent quantitation orifice plate, comprising parallel to arrange and by the first Sptting plate one-piece construction assembled to N Sptting plate that can experimentally need to carry out dismantling or assembling, N is natural number; Each Sptting plate all has spaced multiple pore, and on each Sptting plate, the same side of each pore is sequentially with for pasting or the numbering mark of printing tube hole rows number and row number.Detachable fluorescent quantitation orifice plate described in the utility model, can to overcome in prior art that material use efficiency is low, experiment effect is poor and the defect such as operating process is complicated, to realize, material use efficiency is high, experiment effect good and the simple advantage of operating process.
Description
Technical field
The utility model relates to fluorescent quantitation technical field of experiment equipment, particularly, relates to a kind of detachable fluorescent quantitation orifice plate.
Background technology
In biological experiment, 96 orifice plate right and wrong experiment consumptive material usually.The kind of 96 orifice plates is more, generally comprises for experiment plates such as quantitative fluorescent PCR, cell cultures, enzyme linked immunologicals.
In the experimentation of expression amount detecting gene by the method for fluorescent quantitation, 96 orifice plates for fluorescent quantitation are also one of main experiment consumptive materials.
There is many defects in 96 orifice plates of current commercial type, specific as follows:
(1), due to the sample size difference that each fluorescent quantitation is tested, required reaction pore is also different, does not sometimes need all pores of 96 orifice plates all to add sample.How as a whole 96 orifice plates that current market is sold are, if do not add all pores can cause certain consumptive material waste, if cut out Sptting plate with scissors, experimentation can be made again to become loaded down with trivial details, affect conventional efficient.
(2), although existing 96 orifice plates have numeral or letter mark in each row front end of pore and Ge Lie upper end, each pore is not identified, be still easier to add wrong sample in the process of application of sample.
(3) four angles of existing 96 hole Sptting plates are all identical, being easily inverted, causing the experimental result of instrument record mistake when putting into quantitative real time PCR Instrument.
Realizing in process of the present utility model, contriver finds at least to exist in prior art the defect such as material use efficiency is low, experiment effect is poor and operating process is complicated.
Utility model content
The purpose of this utility model is, for the problems referred to above, proposes a kind of detachable fluorescent quantitation orifice plate, and to realize, material use efficiency is high, experiment effect good and the simple advantage of operating process.
For achieving the above object, the technical solution adopted in the utility model is: a kind of detachable fluorescent quantitation orifice plate, comprising parallel to arrange and by the first Sptting plate one-piece construction assembled to N Sptting plate that can experimentally need to carry out dismantling or assembling, N is natural number; Each Sptting plate all has spacedly has multiple pore, and on each Sptting plate, the same side of each pore is sequentially with for pasting or the numbering mark of printing tube hole rows number and row number.
Further, in described Sptting plate one-piece construction, N number of Sptting plate is plugged in together to N Sptting plate order from the first Sptting plate, and the upper right corner of N Sptting plate is provided with the unfilled corner in the direction for distinguishing reaction slab integral structure.
Further, in described Sptting plate one-piece construction, be provided with mistake proofing graticule in the junction of adjacent two Sptting plates.
Further, in described Sptting plate one-piece construction, the thickness of each Sptting plate right side edge is greater than the thickness of each Sptting plate left side edge.
Further, in described Sptting plate one-piece construction:
First to N Sptting plate, by matching the groove and fin grafting or dismounting that arrange between adjacent reaction plate;
On the right side of N-1 Sptting plate, along the length direction of Sptting plate be provided with N Sptting plate on the left of the groove that matches of fin;
In the left side of N Sptting plate, along the length direction of Sptting plate be provided with N-1 Sptting plate on the right side of the fin of groove match.
Further, described N is the natural number of 2 to 4, and Sptting plate one-piece construction forms 96 orifice plates by 24 orifice plates of four 3 row × 8 row of detachable setting.
The detachable fluorescent quantitation orifice plate of each embodiment of the utility model, owing to comprising parallel to arrange and by the first Sptting plate one-piece construction assembled to N Sptting plate that can experimentally need to carry out dismantling or assembling, N is natural number; Each Sptting plate all has spaced multiple pore, and on each Sptting plate, the same side of each pore is sequentially with for pasting or the numbering mark of printing tube hole rows number and row number; Thus the defect that in prior art, material use efficiency is low, experiment effect is poor and operating process is complicated can be overcome, to realize, material use efficiency is high, experiment effect good and the simple advantage of operating process.
Other features and advantages of the utility model will be set forth in the following description, and, partly become apparent from specification sheets, or understand by implementing the utility model.
Below by drawings and Examples, the technical solution of the utility model is described in further detail.
Accompanying drawing explanation
Accompanying drawing is used to provide further understanding of the present utility model, and forms a part for specification sheets, is used from explanation the utility model, does not form restriction of the present utility model with embodiment one of the present utility model.In the accompanying drawings:
Fig. 1 is the Sptting plate one-piece construction figure of the detachable fluorescent quantitation orifice plate of the utility model;
Fig. 2 is the Sptting plate vertical view of the detachable fluorescent quantitation orifice plate of the utility model;
Fig. 3 is the Sptting plate junction sectional view of the detachable fluorescent quantitation orifice plate of the utility model.
By reference to the accompanying drawings, in the utility model embodiment, Reference numeral is as follows:
1-plate body; 2-junction or mistake proofing graticule (as mistake proofing black line); 3-pore; 4-unfilled corner; 5-body; 6-numbering mark.
Embodiment
Below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the utility model, and be not used in restriction the utility model.
According to the utility model embodiment, as shown in Figure 1, Figure 2 and Figure 3, provide a kind of detachable fluorescent quantitation orifice plate, be specially a kind of detachable fluorescent quantitation 96 orifice plate.
The detachable fluorescent quantitation orifice plate of the present embodiment comprises parallel setting and by the first Sptting plate one-piece construction assembled to N Sptting plate that can experimentally need to carry out dismantling or assembling, N is natural number; Each Sptting plate (as plate body 1) all has spaced multiple pore (as pore 3), be body (as body 5) below pore, on each Sptting plate, the same side of each pore is sequentially with for pasting or the numbering mark (as numbering mark 6) of printing tube hole rows number and row number.Such as, N is 4, and Sptting plate one-piece construction forms 96 orifice plates by 24 orifice plates of four 3 row × 8 row of detachable setting.Pore is not used to plant reaction body, and reaction carries out in the pipe directly on Sptting plate.
In above-mentioned Sptting plate one-piece construction, N number of Sptting plate is plugged in together to N Sptting plate order from the first Sptting plate, and the upper right corner of N Sptting plate is provided with the unfilled corner (as unfilled corner 4) in the direction for distinguishing reaction slab integral structure; In Sptting plate one-piece construction, be provided with mistake proofing graticule (as junction or mistake proofing black line 2) in the junction of adjacent two Sptting plates; In Sptting plate one-piece construction, the thickness of each Sptting plate right side edge is greater than the thickness of each Sptting plate left side edge.
In above-mentioned Sptting plate one-piece construction: on the right side of the first Sptting plate, the length direction along the first Sptting plate is provided with groove; First to N Sptting plate, by matching the groove and fin grafting or dismounting that arrange between adjacent reaction plate; On the right side of N-1 Sptting plate, along the length direction of Sptting plate be provided with N Sptting plate on the left of the groove that matches of fin;
In the left side of N Sptting plate, along the length direction of Sptting plate be provided with N-1 Sptting plate on the right side of the fin of groove match.
Main purpose of the present invention is, experimental needs are combined into the Sptting plate with desirable pore number, adds sample quickly and accurately in pore, by correct direction, the Sptting plate being added with sample is put into quantitative real time PCR Instrument.The key issue mainly solved has following 3 points:
(1) Sptting plate of the present invention detachably becomes four 3 24 orifice plates arranging × 8 row (because the repeat number of experiment is generally 3, the often row of this plate can add identical sample), can experimentally need to dismantle it and assemble, the waste of experiment consumptive material can not be caused, experimentation can not be made again to become too loaded down with trivial details.
(2) the edge of 24 orifice plates of aforenoted remov marks with black line, and there is the combination number of letter and number the top of the pore simultaneously, and this makes to be not easy to add wrong sample in the process of application of sample.
(3) when being combined into 96 complete orifice plate, its top-right angle is cut, and can identifying its direction well, being not easy to be squeezed when putting into quantitative real time PCR Instrument; When the Sptting plate be combined into is not 96 complete hole (may be 24,48 or 72 holes), because the edge on the left of Sptting plate is wider than right side, be also not easy Sptting plate is inverted.
Technical scheme of the present invention, has following characteristics:
(1) mixing needs the fluorescent quantitation reaction system of reaction.
(2) combine or remove the fluorescent quantitation Sptting plate needing size.
(3) experimentally require and the error proofing feature (have above black line and pore and have numbering) of Sptting plate, fluorescent quantitation laboratory sample is added in Sptting plate.
(4) change fluorescent quantitation reaction instrument, fluorescent quantitation response procedures is set, and runs response procedures.
(5) analyze the quantitative data of gained.
Compared with prior art, technical scheme of the present invention, the beneficial effect that at least can reach is:
(1) Sptting plate of the present invention detachably becomes four 3 24 orifice plates arranging × 8 row, can experimentally need to have dismantled assembling to it, can not cause the waste of experiment consumptive material, experimentation can not be made again to become too loaded down with trivial details.
(2) the edge of dismountable 24 orifice plates marks with black line, and there is the combination number of letter and number the top of the pore simultaneously, and this makes to be not easy to add wrong sample in the process of application of sample.
(3) its top-right angle of 96 complete orifice plates is cut, and can identifying its direction well, being not easy to be squeezed when putting into quantitative real time PCR Instrument; When the Sptting plate be combined into is not 96 complete hole (may be 24,48 or 72 holes), because the edge on the left of Sptting plate is wider than right side, be also not easy Sptting plate is inverted.
Last it is noted that the foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, although be described in detail the utility model with reference to previous embodiment, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.All within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (6)
1. a detachable fluorescent quantitation orifice plate, is characterized in that, comprising parallel to arrange and by the first Sptting plate one-piece construction assembled to N Sptting plate that can experimentally need to carry out dismantling or assembling, N is natural number; Each Sptting plate all has spaced multiple pore, and on each Sptting plate, the same side of each pore is sequentially with for pasting or the numbering mark of printing tube hole rows number and row number.
2. detachable fluorescent quantitation orifice plate according to claim 1, it is characterized in that, in described Sptting plate one-piece construction, N number of Sptting plate is plugged in together to N Sptting plate order from the first Sptting plate, and the upper right corner of N Sptting plate is provided with the unfilled corner in the direction for distinguishing reaction slab integral structure.
3. detachable fluorescent quantitation orifice plate according to claim 1 and 2, is characterized in that, in described Sptting plate one-piece construction, is provided with mistake proofing graticule in the junction of adjacent two Sptting plates.
4. detachable fluorescent quantitation orifice plate according to claim 1 and 2, is characterized in that, in described Sptting plate one-piece construction, the thickness of each Sptting plate right side edge is greater than the thickness of each Sptting plate left side edge.
5. detachable fluorescent quantitation orifice plate according to claim 1 and 2, is characterized in that, in described Sptting plate one-piece construction:
First to N Sptting plate, by matching the groove and fin grafting or dismounting that arrange between adjacent reaction plate;
On the right side of N-1 Sptting plate, along the length direction of Sptting plate be provided with N Sptting plate on the left of the groove that matches of fin;
In the left side of N Sptting plate, along the length direction of Sptting plate be provided with N-1 Sptting plate on the right side of the fin of groove match.
6. detachable fluorescent quantitation orifice plate according to claim 1 and 2, is characterized in that, described N is the natural number of 2 to 4, and Sptting plate one-piece construction forms 96 orifice plates by 24 orifice plates of four 3 row × 8 row of detachable setting.
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CN201520308918.2U CN204625613U (en) | 2015-05-14 | 2015-05-14 | A kind of detachable fluorescent quantitation orifice plate |
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CN201520308918.2U CN204625613U (en) | 2015-05-14 | 2015-05-14 | A kind of detachable fluorescent quantitation orifice plate |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106198960A (en) * | 2016-07-08 | 2016-12-07 | 何韶衡 | A kind of ELISA Plate freely assembled |
CN109439529A (en) * | 2018-12-29 | 2019-03-08 | 中国人民解放军军事科学院军事医学研究院 | A kind of reaction tube carrying out multiple nucleic acid amplification |
-
2015
- 2015-05-14 CN CN201520308918.2U patent/CN204625613U/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106198960A (en) * | 2016-07-08 | 2016-12-07 | 何韶衡 | A kind of ELISA Plate freely assembled |
CN109439529A (en) * | 2018-12-29 | 2019-03-08 | 中国人民解放军军事科学院军事医学研究院 | A kind of reaction tube carrying out multiple nucleic acid amplification |
WO2020135873A1 (en) * | 2018-12-29 | 2020-07-02 | 中国人民解放军军事科学院军事医学研究院 | Reaction tube for multiple nucleic acid amplification |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150909 Termination date: 20160514 |