CN204594882U - A kind of living body fluorescent microscopic imaging device - Google Patents
A kind of living body fluorescent microscopic imaging device Download PDFInfo
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- CN204594882U CN204594882U CN201520280227.6U CN201520280227U CN204594882U CN 204594882 U CN204594882 U CN 204594882U CN 201520280227 U CN201520280227 U CN 201520280227U CN 204594882 U CN204594882 U CN 204594882U
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Abstract
The utility model discloses a kind of living body fluorescent microscopic imaging device, comprise the miniature fluorescent microscope, data processing module and the electric slip ring that are fixed on somatoscopy object; Described miniature fluorescent microscope is connected by data line with described data processing module, described data processing module forms the real-time fluorescence micro-image of somatoscopy object for sending view data, described data processing module is connected by electric wire with slip ring, and described slip ring is used for powering to data processing module.The utility model movable data processing module used can ensure that the free activity of animal can not be obstructed because of the winding of data line.
Description
Technical field
The utility model belongs to fluorescent microscopic imaging field, more specifically, relates to a kind of fluorescent microscopic imaging device for free moving animals.
Background technology
To vivo biological tissue imaging, it is an important means in life science field.And wherein to the real time imagery of live body brain cell, become the important means contacted of zoologizeing between behavior and cerebral neuron inside Neuscience, in experimentation, give animal stimulation makes animal make behavior, and to observe in animal brain district specific region neuron produce reaction, can set up like this animal behavior and its related Neurons distribute between contact.
By measuring in active procedure, the action potential change of animal's nerve cells, therewith whether behavior is relevant to detect this neurocyte.The light signal excited due to electric indicator is too weak, and poor selectivity is unsatisfactory in the effect of clinical testing, therefore researchist can use the change that calcon-carboxylic acid carrys out indirect inspection action potential usually.Calcium ion is maintain intraor extracellular electrochemical gradient in intracellular effect, intracellular second messenger, action potential change frequency is relevant with calcium ion concentration change, when neurocyte activates the change of generation action potential in action process, the increase of intracellular calcium concentration can be caused, now calcium ion is combined with calcon-carboxylic acid and produces fluorescin, and fluorescence excitation under the stimulation of extraneous illumination, just can detect fluorescence signal by imaging device.
At neuroscience field, studying that this animal behavior and functional neurosurgery unit associates is method mainly through live body real time imagery.At present, a kind of method utilizing miniature fluorescence microscopy device animal to be carried out to live body real time imagery has been developed in the industry.Miniature fluorescence microscopy device volume is little, and mirror body can be fixed on mouse head, and its weight can not have influence on the activity of mouse.But current miniature its data of fluorescence microscopy device are transmitted and relied on data line, can produce moment of torsion when data line is wound around, hinder the freely activity of mouse in large area space, such as existing mode does not just support the maze experiment of mouse.
Utility model content
For above defect or the Improvement requirement of prior art, the utility model provides a kind of living body fluorescent microscopic imaging device, its object is to by slip ring or wireless signal transmission techniques, solve the technical matters that existing miniature fluorescence restriction living animal is freely movable thus.
For achieving the above object, according to an aspect of the present utility model, provide a kind of living body fluorescent microscopic imaging device, comprise the miniature fluorescent microscope, data processing module and the electric slip ring that are fixed on somatoscopy object; Described miniature fluorescent microscope is connected by data line with described data processing module, described data processing module forms the real-time fluorescence micro-image of somatoscopy object for sending view data, described data processing module is connected by electric wire with slip ring, and described slip ring is used for powering to data processing module.
Preferably, described living body fluorescent microscopic imaging device, it also comprises oscillating bearing, and described oscillating bearing is connected with electric slip ring, for providing three axle rotational freedoms.
Preferably, described living body fluorescent microscopic imaging device, its data processing module is wireless data processing module.
Preferably, described living body fluorescent microscopic imaging device, its data processing module is cable data processing module, and described electric slip ring is used for transmission video signal.
In general, the above technical scheme conceived by the utility model compared with prior art, can obtain following beneficial effect: the utility model movable data processing module used can ensure that the free activity of animal can not be obstructed because of the winding of data line.
Accompanying drawing explanation
Fig. 1 is the utility model structural representation;
Fig. 2 is the structural representation that oscillating bearing is connected with electric slip ring.
In all of the figs, identical Reference numeral is used for representing identical element or structure, and wherein: 1 is miniature fluorescent microscope, 2 is data processing module, and 3 is electric slip ring, and 4 is oscillating bearing, and 41 is oscillating bearing inner ring, and 42 is oscillating bearing outer ring.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.In addition, if below in described each embodiment of the utility model involved technical characteristic do not form conflict each other and just can mutually combine.
The living body fluorescent microscopic imaging device that the utility model provides, comprises the miniature fluorescent microscope, data processing module and the electric slip ring that are fixed on somatoscopy object; Described miniature fluorescent microscope is connected by data line with described data processing module, described data processing module forms the real-time fluorescence micro-image of somatoscopy object for sending view data, described data processing module is connected by electric wire with slip ring, and described slip ring is used for powering to data processing module.Also comprise oscillating bearing, described oscillating bearing is connected with electric slip ring, for providing three axle rotational freedoms.
Described miniature fluorescent microscope, for sample imaging, data processing module, for receiving real time imagery data and being transferred to host computer, ensures the freely movable of somatoscopy object.
Described miniature fluorescent microscope, preferably includes microscope illuminator, and some Individual optical components and parts and the face battle array photodetector such as catoptron, miniature object lens, optical filtering; Described optical component is fixed together by mechanical cover.Microscope illuminator can use monochromatic LED, also can use the outside laser of coupling fiber.The light source of configuration different wave length and filter set, can realize the fluorescence imaging based on different fluorescin.Described miniature object lens are made up of gradient-index lens, and concrete selection is relevant with imaging demand.Described battle array photodetector, be positioned on described microscopical imaging plane, for data acquisition, data transmitted data processing module by wireless or wired mode.
Described data processing module, can be wired or infinite data processing module.The data line of the data processing module of wired forms is connected with electric slip ring group with electric wire, and described electric slip ring is used for transmission video signal, and the data processing module of wireless sends the data to host computer by wireless connections, is connected with electric slip ring by electric wire.
Be below embodiment:
Embodiment 1
A kind of living body fluorescent microscopic imaging device, as shown in Figure 1, comprises the miniature fluorescent microscope 1 be fixed on somatoscopy object, data processing module 2 and electric slip ring 3; Described miniature fluorescent microscope 1 is connected by data line with described data processing module 2, described data processing module 2 forms the real-time fluorescence micro-image of somatoscopy object for sending view data, described data processing module 2 is connected by electric wire with slip ring, and described slip ring is used for powering to data processing module 2.Described living body fluorescent microscopic imaging device, comprises oscillating bearing 4, and described oscillating bearing 4 is connected with electric slip ring 3, for providing three axle rotational freedoms.
Described data processing module 2 is wireless data processing module 2.
Embodiment 2
A kind of living body fluorescent microscopic imaging device, as shown in Figure 1, comprises the miniature fluorescent microscope 1 be fixed on somatoscopy object, data processing module 2 and electric slip ring 3; Described miniature fluorescent microscope 1 is connected by data line with described data processing module 2, described data processing module 2 forms the real-time fluorescence micro-image of somatoscopy object for sending view data, described data processing module 2 is connected by electric wire with slip ring, and described slip ring is used for powering to data processing module 2.Described slip ring type fluorescent microscopic imaging device, comprise oscillating bearing 4, described oscillating bearing 4 is connected with electric slip ring 3, for providing three axle rotational freedoms.
Described data processing module 2 is by first data processing module 2, described electric slip ring 3 for transmission video signal, as shown in Figure 2.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (4)
1. a living body fluorescent microscopic imaging device, is characterized in that, comprises the miniature fluorescent microscope (1) be fixed on somatoscopy object, data processing module (2) and electric slip ring (3); Described miniature fluorescent microscope (1) is connected by data line with described data processing module (2), described data processing module (2) forms the real-time fluorescence micro-image of somatoscopy object for sending view data, described data processing module (2) is connected by electric wire with slip ring, and described slip ring is used for powering to data processing module (2).
2. living body fluorescent microscopic imaging device as claimed in claim 1, it is characterized in that, also comprise oscillating bearing (4), described oscillating bearing (4) is connected with electric slip ring (3), for providing three axle rotational freedoms.
3. living body fluorescent microscopic imaging device as claimed in claim 1, it is characterized in that, described data processing module (2) is wireless data processing module (2).
4. living body fluorescent microscopic imaging device as claimed in claim 1, it is characterized in that, described data processing module (2) is cable data processing module (2), and described electric slip ring (3) is transmission of video electric slip ring (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520280227.6U CN204594882U (en) | 2015-05-04 | 2015-05-04 | A kind of living body fluorescent microscopic imaging device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520280227.6U CN204594882U (en) | 2015-05-04 | 2015-05-04 | A kind of living body fluorescent microscopic imaging device |
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| CN204594882U true CN204594882U (en) | 2015-08-26 |
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| CN201520280227.6U Expired - Fee Related CN204594882U (en) | 2015-05-04 | 2015-05-04 | A kind of living body fluorescent microscopic imaging device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110584616A (en) * | 2019-10-18 | 2019-12-20 | 南方科技大学 | Dual-mode imaging microscope system |
| CN113827192A (en) * | 2021-10-08 | 2021-12-24 | 北京大学 | Wireless micro fluorescent microscopic imaging device and application thereof |
-
2015
- 2015-05-04 CN CN201520280227.6U patent/CN204594882U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110584616A (en) * | 2019-10-18 | 2019-12-20 | 南方科技大学 | Dual-mode imaging microscope system |
| CN113827192A (en) * | 2021-10-08 | 2021-12-24 | 北京大学 | Wireless micro fluorescent microscopic imaging device and application thereof |
| CN113827192B (en) * | 2021-10-08 | 2023-12-05 | 北京大学 | Wireless micro fluorescent microscopic imaging device and application thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150826 |
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| CF01 | Termination of patent right due to non-payment of annual fee |