CN204569932U - One wins card parvovirus test kit for detecting 2 type ungulatess - Google Patents
One wins card parvovirus test kit for detecting 2 type ungulatess Download PDFInfo
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- CN204569932U CN204569932U CN201520088933.0U CN201520088933U CN204569932U CN 204569932 U CN204569932 U CN 204569932U CN 201520088933 U CN201520088933 U CN 201520088933U CN 204569932 U CN204569932 U CN 204569932U
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Abstract
The open one of the utility model wins card parvovirus test kit for detecting 2 type ungulatess, comprises the rectangular box of open top and covers the top cover at box body top, being provided with multiple dismountable fixing strip in above-mentioned box body; Above-mentioned fixing strip is parallel to the bottom of above-mentioned box body; Above-mentioned fixing strip is provided with at least one fixed orifices for fixating reagent; Shockproof assembly is provided with between above-mentioned fixing strip and the bottom of box body.By the shockproof assembly that the utility model designs, the test tube in test kit can be made when box body rocks by a small margin to keep static, thus maintain the activity of reagent, improve 2 type ungulatess and win card parvovirus accuracy of detection.
Description
Technical field
The utility model relates to detection kit, and concrete one wins card parvovirus test kit for detecting 2 type ungulatess.
Background technology
In order to save time, easy to use, increasing experimental procedure and detection method are developed to test kit, but traditional test kit is simple monolayer package box, and internal space is closed single structure, and all the elements thing is stacked in box.In use, technician is difficult to obtain required component rapidly and accurately, makes troubles to actually operating, and in Product transport process, the various component in the inside easily occurs collision mutually and causes damage, requires higher to transport condition.The probe (sequence is CGATCCACCCGCCG) winning card parvovirus (PBoV2) in particular for detecting 2 type ungulatess easily lost efficacy under the condition of long-term vibration, made detected result occur false positive.Therefore need badly and a kind of test kit preventing shaking is provided.
Utility model content
In view of this, the utility model discloses and a kind ofly improves the test kit that 2 type ungulatess win card parvovirus accuracy of detection.The purpose of this utility model is achieved through the following technical solutions:
One wins card parvovirus test kit for detecting 2 type ungulatess, comprises the rectangular box of open top and covers the top cover at box body top, being provided with multiple dismountable fixing strip in described box body; Described fixing strip is parallel to the bottom of described box body; Described fixing strip is provided with at least one fixed orifices for fixating reagent; Shockproof assembly is provided with between described fixing strip and the bottom of box body.
Dismountable fixing strip can improve the range of application of test kit, and for providing condition according to certain order arrangement test tube, prevents test tube chaotic with to improve detection efficiency.The test tube being contained with the reagent such as Auele Specific Primer, fluorescent probe, substrate is fixed in fixed orifices, prevents test tube displacement, collision.According to the research of designer, the vibrations of test tube end are larger for probe activity impact.Therefore the utility model arranges shockproof assembly at fixing strip and box body especially, and for preventing test tube, especially the end of test tube rocks.
Further, described shockproof assembly comprises flexible backing plate, is arranged on the cavity layer below flexible backing plate and the surge chamber under being arranged on cavity layer; Described cavity layer comprises multiple triangular prism-shaped cavity be crisscross arranged; The edge of the bottom of described surge chamber is provided with multiple rotating connecting rod, the end of connecting rod and the bottom-hinged of cavity layer; The center of the bottom of described cavity layer is also provided with multiple buffer spring.
Described flexible backing plate is optional to be made with any one flexible materials, as viscose sponge, silica gel, rubber etc.Cavity layer can intercept the vibrations that box surface transmits on the one hand, can support described flexible backing plate on the other hand, cannot fix test tube end to avoid flexible backing plate too soft.The effect of connecting rod maintains the static of test tube when box body transverse shakiness with inertia.The effect of buffer spring maintains the static of test tube when box body longitudinally rocks with inertia.By the shockproof assembly that the utility model designs, the test tube in test kit can be made when box body rocks by a small margin to keep static, thus maintain the activity of reagent, improve 2 type ungulatess and win card parvovirus accuracy of detection.
Further, described top cover is connected by seal assembly with box body; Described seal assembly comprises the annular projection that is looped around box body top outer and is arranged on the groove coordinated inside top cover, with convex; Described groove surfaces is coated with elastic sealing layer; The cross section of described convex is semicircle; The bottom of described top cover is provided with outstanding compression cambered surface.
During top closure, projection embeds in described groove.Under the extruding of projection and groove, elastic sealing layer generation elastic deformation and improve the stopping property of test kit, prevents reagent contaminated, rotten.Outstanding compression cambered surface can squeeze out the air in box body, thus is pressed on box body by top cover by normal atmosphere.Elastic sealing layer can select rubber to make.
Described flexible backing plate top is provided with the pickup groove corresponding with described fixed orifices; Be provided with shakeproof layer between described fixing strip and top cover, described shakeproof layer is provided with the shockproof passage corresponding with fixed orifices.
The inner side of described box body is provided with multiple convex, and described fixing strip crossbearer is on convex.
Preferably, described fixing strip quantity is 4, is followed successively by substrate fixing strip, taq enzyme fixing strip, Auele Specific Primer fixing strip and fluorescent probe fixing strip.
Different reagent is fixed on independent fixing strip, can prevent the mixing of reagent, improves the identification of reagent.
The top of described fixing strip is provided with cog region.
Preferably, cog region is the rectangular recess being arranged on fixing strip surface, and the coating of different colours can be filled in inside.Filling paint in the cog region of depression, can avoid the wearing and tearing of coating and None-identified reagent, or prevent coating pollution reagent.
The bocavirus that bocavirus (Bocavirus) is under the jurisdiction of Parvoviridae parvovirus subfamily belongs to.Parvovirus comprises dense Chordopoxvirinae and Parvoviridae subfamily, the former main infection insect, latter main infection vertebrates.Parvovirus subfamily is divided into again 6 genus such as parvovirus genus, bocavirus genus, red Tobamovirus, dependovirus, ermine aleutian disease virus genus.Pig bocavirus (Porcine Bocavirus, PBoV win card parvovirus also known as pig) is then that the bocavirus belonged to wherein belongs to, for sub-thread wire is without cyst membrane DNA virus, genome, at about 5.2kb, comprises 3 open reading frame (ORFs), be respectively NS1, NP1 and V P1/VP2.2013 ICTV (http: //www. ictvdh. org) new suggestion is proposed to the somatotype of pig bocavirus, propose with NSl gene coding amino acid homology, pig bocavirus is divided into 4 populations: Ungulate bocaparvovirus 2 type (2 type ungulatess win card parvovirus, and UBoV2 comprises PBoVl, PBoV2, PBoV mono-A6), Ungulate bocaparvovirus 3 type comprise PBoV-SX, Ungulate bocaparvovirus 4 type, Ungulate bocaparvovirus 5 type.2 type ungulates are wherein won card parvovirus and include PBoV1, PBoV2, PBoV-A6 tri-kinds of genotype, these three kinds of genotype have and infect strong, the popular feature such as extensively of efficiency, early stage diagnosis is conducive to preventing in time swinery, avoids extensive infection to cause serious financial loss.Designer designs specific amplification and detects the primer (upstream: GCCGATTCTG ATTTTCTCGAA of UBoV2; Downstream: TGAGTCCAAA CACGCCCTTT) and fluorescent probe (CGATCCACCC GCCG).This primer and probe have good Sensitivity and Specificity, but it is comparatively responsive to reaction system, and the character of its probe, primer and template is unstable, violent vibrations cannot be had diversity by the sequence of primer amplification or amplified production by causing template, make diagnostic result occur deviation.Test kit of the present utility model is optimized design for this reaction system specially, prevents reagent sex change because of vibrations in transportation, is conducive to each reagent and keeps reactive behavior for a long time.
The utility model, relative to prior art, has following beneficial effect:
1. the shockproof assembly of the utility model setting, by the cooperation of flexible backing plate, cavity layer, surge chamber, the test tube in test kit can be made when box body rocks by a small margin to keep static, thus maintain the activity of reagent, improve 2 type ungulatess and win card parvovirus accuracy of detection;
2. different reagent is fixed on independent fixing strip by the utility model, can prevent the mixing of reagent, improves the identification of reagent, thus effectively improves detection efficiency.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of another embodiment of the utility model.
Fig. 3 is the utility model downward projection figure.
Embodiment
The utility model to be described in further detail below in conjunction with accompanying drawing and embodiment for the ease of it will be appreciated by those skilled in the art that:
Embodiment 1
The present embodiment provides a kind of and wins card parvovirus test kit for detecting 2 type ungulatess, as shown in Figure 1, comprises the rectangular box 1 of open top and covers the top cover 2 at box body top, being provided with 4 dismountable fixing strips 11 in above-mentioned box body; Above-mentioned fixing strip is parallel to the bottom of above-mentioned box body; Above-mentioned fixing strip is provided with 6 fixed orificess 111 for fixating reagent; Shockproof assembly is provided with between above-mentioned fixing strip and the bottom of box body.
Above-mentioned shockproof assembly comprises flexible backing plate 31, is arranged on the cavity layer 32 below flexible backing plate and the surge chamber 33 under being arranged on multi-chamber layer; Above-mentioned cavity layer comprises multiple triangular prism-shaped cavity 321 be crisscross arranged; The edge of the bottom of above-mentioned surge chamber is provided with multiple rotating connecting rod 331, the end of connecting rod and the bottom-hinged of cavity layer; The center of the bottom of above-mentioned surge chamber is also provided with multiple buffer spring 332.
Above-mentioned top cover 2 is connected by seal assembly with box body 1; Above-mentioned seal assembly comprises the annular projection 41 that is looped around box body top outer and is arranged on the groove 42 coordinated inside top cover, with convex; Above-mentioned groove surfaces is coated with elastic sealing layer; The cross section of above-mentioned convex is semicircle; The bottom of above-mentioned top cover is provided with outstanding compression cambered surface 43.
Above-mentioned flexible backing plate top is provided with the pickup groove 311 corresponding with above-mentioned fixed orifices.
The inner side of above-mentioned box body is provided with multiple convex 12, and above-mentioned fixing strip 11 crossbearer is on convex 12.
Embodiment 2
The present embodiment provides a kind of and wins card parvovirus test kit for detecting 2 type ungulatess, as shown in Figure 2, comprises the rectangular box 1 of open top and covers the top cover 2 at box body top, being provided with 4 dismountable fixing strips 11 in above-mentioned box body; Above-mentioned fixing strip is parallel to the bottom of above-mentioned box body; Above-mentioned fixing strip is provided with 6 fixed orificess 111 for fixating reagent; Shockproof assembly is provided with between above-mentioned fixing strip and the bottom of box body.
Above-mentioned shockproof assembly comprises flexible backing plate 31, is arranged on the cavity layer 32 below flexible backing plate and the surge chamber 33 under being arranged on multi-chamber layer; Above-mentioned cavity layer comprises multiple triangular prism-shaped cavity 321 be crisscross arranged; The edge of the bottom of above-mentioned surge chamber is provided with multiple rotating connecting rod 331, the end of connecting rod and the bottom-hinged of cavity layer; The center of the bottom of above-mentioned surge chamber is also provided with multiple buffer spring 332.
Above-mentioned top cover 2 is connected by seal assembly with box body 1; Above-mentioned seal assembly comprises the annular projection 41 that is looped around box body top outer and is arranged on the groove 42 coordinated inside top cover, with convex; Above-mentioned groove surfaces is coated with elastic sealing layer; The cross section of above-mentioned convex is semicircle; The bottom of above-mentioned top cover is provided with outstanding compression cambered surface 43.
Above-mentioned flexible backing plate top is provided with the pickup groove 311 corresponding with above-mentioned fixed orifices; Be provided with shakeproof layer 51 between above-mentioned fixing strip and top cover, above-mentioned shakeproof layer is provided with the shockproof passage 52 corresponding with fixed orifices.
The inner side of above-mentioned box body is provided with multiple convex 12, and above-mentioned fixing strip 11 crossbearer is on convex 12.
Fixing strip quantity as above-mentioned in Fig. 3 is 4, is followed successively by substrate fixing strip 112, taq enzyme fixing strip 113, Auele Specific Primer fixing strip 114 and fluorescent probe fixing strip 115.
Preferably, cog region 6 is also provided with in the middle part of fixing strip.
Be more than wherein specific implementation of the present utility model, it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these apparent replacement forms all belong to protection domain of the present utility model.
Claims (7)
1. winning a card parvovirus test kit for detecting 2 type ungulatess, comprising the rectangular box of open top and covering the top cover at box body top, it is characterized in that: in described box body, being provided with multiple dismountable fixing strip; Described fixing strip is parallel to the bottom of described box body; Described fixing strip is provided with at least one fixed orifices for fixating reagent; Shockproof assembly is provided with between described fixing strip and the bottom of box body.
2. test kit according to claim 1, is characterized in that: described shockproof assembly comprises flexible backing plate, is arranged on the cavity layer below flexible backing plate and the surge chamber under being arranged on cavity layer; Described cavity layer comprises multiple triangular prism-shaped cavity be crisscross arranged; The edge of the bottom of described surge chamber is provided with multiple rotating connecting rod, the end of connecting rod and the bottom-hinged of cavity layer; The center of the bottom of described cavity layer is also provided with multiple buffer spring.
3. test kit according to claim 1, is characterized in that: described top cover is connected by seal assembly with box body; Described seal assembly comprises the annular projection that is looped around box body top outer and is arranged on the groove coordinated inside top cover, with convex; Described groove surfaces is coated with elastic sealing layer; The cross section of described convex is semicircle; The bottom of described top cover is provided with outstanding compression cambered surface.
4. test kit according to claim 2, is characterized in that: described flexible backing plate top is provided with the pickup groove corresponding with described fixed orifices; Be provided with shakeproof layer between described fixing strip and top cover, described shakeproof layer is provided with the shockproof passage corresponding with fixed orifices.
5. the test kit according to any one of claim 1-4, is characterized in that: the inner side of described box body is provided with multiple convex, and described fixing strip crossbearer is on convex.
6. test kit according to claim 5, is characterized in that: described fixing strip quantity is 4, is followed successively by substrate fixing strip, taq enzyme fixing strip, Auele Specific Primer fixing strip and fluorescent probe fixing strip.
7. test kit according to claim 6, is characterized in that: the top of described fixing strip is provided with cog region.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520088933.0U CN204569932U (en) | 2015-02-09 | 2015-02-09 | One wins card parvovirus test kit for detecting 2 type ungulatess |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520088933.0U CN204569932U (en) | 2015-02-09 | 2015-02-09 | One wins card parvovirus test kit for detecting 2 type ungulatess |
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| Publication Number | Publication Date |
|---|---|
| CN204569932U true CN204569932U (en) | 2015-08-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520088933.0U Expired - Fee Related CN204569932U (en) | 2015-02-09 | 2015-02-09 | One wins card parvovirus test kit for detecting 2 type ungulatess |
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| CN (1) | CN204569932U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107191538A (en) * | 2017-06-08 | 2017-09-22 | 成都锐奕信息技术有限公司 | Improve the damping device of card form terminal steady |
| CN108051366A (en) * | 2017-12-13 | 2018-05-18 | 中山市凡卓生物科技有限公司 | A kind of kit for testing Thyroxine Contents |
| CN108165479A (en) * | 2018-01-15 | 2018-06-15 | 王辉 | A kind of kit for being used to detect Peripheral Circulation tumour cell |
| CN114701736A (en) * | 2022-04-27 | 2022-07-05 | 北京大学第三医院（北京大学第三临床医学院） | Respiratory virus detection kit |
-
2015
- 2015-02-09 CN CN201520088933.0U patent/CN204569932U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107191538A (en) * | 2017-06-08 | 2017-09-22 | 成都锐奕信息技术有限公司 | Improve the damping device of card form terminal steady |
| CN108051366A (en) * | 2017-12-13 | 2018-05-18 | 中山市凡卓生物科技有限公司 | A kind of kit for testing Thyroxine Contents |
| CN108165479A (en) * | 2018-01-15 | 2018-06-15 | 王辉 | A kind of kit for being used to detect Peripheral Circulation tumour cell |
| CN114701736A (en) * | 2022-04-27 | 2022-07-05 | 北京大学第三医院（北京大学第三临床医学院） | Respiratory virus detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150819 Termination date: 20200209 |