CN204569897U - A kind of human sperm preferably with bionic device in vitro fertilization - Google Patents
A kind of human sperm preferably with bionic device in vitro fertilization Download PDFInfo
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- CN204569897U CN204569897U CN201520173107.6U CN201520173107U CN204569897U CN 204569897 U CN204569897 U CN 204569897U CN 201520173107 U CN201520173107 U CN 201520173107U CN 204569897 U CN204569897 U CN 204569897U
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- bionical
- fertilization
- uterus
- pond
- sperm
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- 230000004720 fertilization Effects 0.000 title claims abstract description 71
- 238000000338 in vitro Methods 0.000 title claims abstract description 20
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 13
- 210000004291 uterus Anatomy 0.000 claims abstract description 30
- 210000000582 semen Anatomy 0.000 claims abstract description 24
- 210000003101 oviduct Anatomy 0.000 claims abstract description 23
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 14
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 14
- 210000004681 ovum Anatomy 0.000 claims abstract description 14
- 238000011144 upstream manufacturing Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 230000010354 integration Effects 0.000 abstract description 3
- 238000010295 mobile communication Methods 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 description 1
- 239000002975 chemoattractant Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000005659 seminal clot liquefaction Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- NGSFWBMYFKHRBD-UHFFFAOYSA-N sodium;2-hydroxypropanoic acid Chemical compound [Na+].CC(O)C(O)=O NGSFWBMYFKHRBD-UHFFFAOYSA-N 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a kind of human sperm preferably with bionic device in vitro fertilization, comprise fertilization pond, also comprise the bionical uterine tube of bionical uterus, seminal fluid pipe and at least two, bionical uterus is arranged on below fertilization pond, seminal fluid pipe is connected with bionical uterus through fertilization pond, bionical oviducal lower end is connected with the top in bionical uterus, bionical oviducal upper end is connected with the outer rim in fertilization pond, the outer rim of bionical uterine tube upper end open is higher than the outer rim in fertilization pond, and bionical uterine tube is outside equipped with support.Such ovum is in fertilization pond, seminal fluid by seminal fluid pipe to bionical uterus, then sperm Automatic sieve selects high-quality sperm, from bionical uterus, Mobile Communication crosses bionical uterine tube and enters fertilization pond from bionical uterine tube upper end open, high-quality sperm and ovum fertilization, make sperm preferably with integration in vitro fertilization, and sperm preferably with fertilization easy, quick, be sperm preferably with integrated apparatus in vitro fertilization.
Description
Technical field
The utility model relates to human artificial fertilization device, be specifically related to a kind of human sperm preferably with bionic device in vitro fertilization.
Background technology
In the physiology natural fertilization process of inside of human body, sperm is under the luring of uterine neck, uterus and oviducal screening effect and ovum chemoattractant, allows the sperm of high-quality the most arrive the ampulla of uterine tube end, finds ovum and be fertilized.During the infertile treatment of the mankind, need to adopt auxiliary procreation technology, comprise artificial insemination, the methods for the treatment of such as in vitro fertilization, not only need to obtain high-quality sperm time wherein in vitro fertilization, also will mix outward with ovum or ovarian cumulus composite body and be fertilized.External sperm preferably depends on spermatozoa selection technology and fertilization systems with fertilization, and this technology is directly connected to the success or failure of whole assisted reproductive therapy.The spermatozoa selection technology extensively adopted at present has the optimization technique such as upstream technology, density gradient centrifugation, and these technology all exist different shortcomings.Fertilization is also want Jiang Youzhi sperm under microscopic examination to put into be fertilized pond and ovum fertilization, and therefore traditional sperm is preferably separated with fertilization process, also do not have sperm preferably with integrated apparatus in vitro fertilization.
Summary of the invention
Technical problem to be solved in the utility model be to provide a kind of sperm preferably with integration in vitro fertilization, sperm preferably with fertilization easy, fast human sperm preferably with bionic device in vitro fertilization.
The utility model solves the problems of the technologies described above adopted technical scheme: a kind of human sperm preferably with bionic device in vitro fertilization, comprise fertilization pond, also comprise bionical uterus, the bionical uterine tube of seminal fluid pipe and at least two, described bionical uterus is arranged on below described fertilization pond, described seminal fluid pipe is connected with described bionical uterus through described fertilization pond, described bionical oviducal lower end is connected with the top in described bionical uterus, described bionical oviducal upper end is connected with the outer rim in described fertilization pond, the outer rim of described bionical uterine tube upper end open is higher than the outer rim in described fertilization pond, described bionical uterine tube is outside equipped with support.
Lid is provided with above described fertilization pond.
Described lid is provided with ovum add pipe and upstream liquid add pipe, described seminal fluid pipe pass described lid.
Ball-type seminal pool is set to below described bionical uterus.
Compared with prior art, the utility model has the advantage of a kind of human sperm preferably with bionic device in vitro fertilization, comprise fertilization pond, also comprise the bionical uterine tube of bionical uterus, seminal fluid pipe and at least two, bionical uterus is arranged on below fertilization pond, seminal fluid pipe is connected with bionical uterus through fertilization pond, bionical oviducal lower end is connected with the top in bionical uterus, bionical oviducal upper end is connected with the outer rim in fertilization pond, the outer rim of bionical uterine tube upper end open is higher than the outer rim in fertilization pond, and bionical uterine tube is outside equipped with support.Such ovum is in fertilization pond, seminal fluid by seminal fluid pipe to bionical uterus, then sperm Automatic sieve selects high-quality sperm, from bionical uterus, Mobile Communication crosses bionical uterine tube and enters fertilization pond from bionical uterine tube upper end open, high-quality sperm and ovum fertilization, make sperm preferably with integration in vitro fertilization, and sperm preferably with fertilization easy, quick, be sperm preferably with integrated apparatus in vitro fertilization.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is structure schematic top plan view of the present utility model.
Embodiment
Below in conjunction with accompanying drawing embodiment, the utility model is described in further detail.
Embodiment 1
As Fig. 1, a kind of human sperm shown in Fig. 2 preferably with bionic device in vitro fertilization, comprise fertilization pond 1, also comprise bionical uterus 2, the bionical uterine tube 4 of seminal fluid pipe 3 and 8, bionical uterus 2 is arranged on below fertilization pond 1, seminal fluid pipe 3 is connected with bionical uterus 1 through fertilization pond `, the lower end of bionical uterine tube 4 is connected with the top in bionical uterus 2, the upper end of bionical uterine tube 4 is connected with the outer rim in fertilization pond 1, the outer rim of bionical uterine tube 4 upper end open is higher than the outer rim in fertilization pond 1, such high-quality sperm will enter fertilization pond more smoothly, bionical uterine tube 4 is outside equipped with support 5, fertilization is provided with lid 6 above pond 1, lid 6 is provided with ovum and adds pipe 7 and upstream liquid adds pipe 8, seminal fluid pipe 3 is through lid 6, ball-type seminal pool 21 is set to below bionical uterus 2.
Embodiment 2
Substantially the same manner as Example 1, difference is that lid is not provided with ovum and adds pipe and upstream liquid adds pipe, and seminal fluid pipe does not also pass lid, is tip below bionical uterus, and bionical uterine tube is 2 or 6 or 10 etc., does not enumerate at this.
Embodiment 3
Utilize the bionic device of embodiment 1, the time of fertilization, by the 37 degree of preheatings of upstream liquid [upstream liquid formula: 0.1mol/L NaCl(sodium-chlor), 0.4mmol/L KH2PO4(potassium primary phosphate), 2.7mmol/L C
6h
6o
6(glucose), 4.7mmol/L KCl (Repone K), 0.2mmol/L MgSO47H2O(magnesium sulfate heptahydrate), 2mmol/L CaCl22H2O(Calcium dichloride dihydrate), 0.02mol/L Hepes-Na(N-2-hydroxyethyl piperazine-N-2-ethyl sulfonic acid disodium salt), 4mmol/L NaHCO3(sodium bicarbonate), 0.3mmol/L C3H3O3Na(Sodium.alpha.-ketopropionate), 0.34% (v/v) C3H5O3Na (Sodium.alpha.-hydroxypropionate), 0.002% Phenol Red(be phenol red)].Add seminal fluid from seminal fluid pipe after semen liquefaction or semen thawing, then Jiang Shangyou liquid first adds from seminal fluid pipe, note making upstream liquid be full of each bionical oviducal pipeline; Then Jiang's ovum or cumulus complex add pipe through ovum and join fertilization pond, and then upstream liquid adds pipe from upstream liquid and is added to fertilization pond, and the upstream liquid in fertilization pond is communicated with bionical intratubal upstream liquid; 37 degree of static 15-45 minute, each taking-up 5-10 microlitre upstream liquid from bionical uterus and fertilization pond respectively, motility of sperm and preferred efficiency is analyzed under being placed in microscope, preferred efficiency=100% × preferably to progressive sperm number/former seminal fluid in total progressive sperm, compare with common upper reaches method, density gradient centrifugation, the preferred efficiency of present method reaches 92% ± 5.33; Residue seminal fluid continues upstream and fertilization 3-18 hour, and basis of microscopic observation zygote, selects high quality fertilized egg of scophthatmus for implantation or preservation.
Claims (4)
1. a human sperm preferably with bionic device in vitro fertilization, comprise fertilization pond, characterized by further comprising bionical uterus, the bionical uterine tube of seminal fluid pipe and at least two, described bionical uterus is arranged on below described fertilization pond, described seminal fluid pipe is connected with described bionical uterus through described fertilization pond, described bionical oviducal lower end is connected with the top in described bionical uterus, described bionical oviducal upper end is connected with the outer rim in described fertilization pond, the outer rim of described bionical uterine tube upper end open is higher than the outer rim in described fertilization pond, described bionical uterine tube is outside equipped with support.
2. a kind of human sperm as claimed in claim 1 preferably with bionic device in vitro fertilization, it is characterized in that being provided with lid above described fertilization pond.
3. a kind of human sperm as claimed in claim 2 preferably with bionic device in vitro fertilization, it is characterized in that described lid is provided with ovum and add pipe and upstream liquid adds pipe, described seminal fluid pipe passes described lid.
4. a kind of human sperm as claimed in claim 1 preferably with bionic device in vitro fertilization, it is characterized in that being set to ball-type seminal pool below described bionical uterus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520173107.6U CN204569897U (en) | 2015-03-26 | 2015-03-26 | A kind of human sperm preferably with bionic device in vitro fertilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520173107.6U CN204569897U (en) | 2015-03-26 | 2015-03-26 | A kind of human sperm preferably with bionic device in vitro fertilization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204569897U true CN204569897U (en) | 2015-08-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520173107.6U Active CN204569897U (en) | 2015-03-26 | 2015-03-26 | A kind of human sperm preferably with bionic device in vitro fertilization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204569897U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762192A (en) * | 2015-03-26 | 2015-07-08 | 浙江星博生物科技有限公司 | Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization |
| CN109468226A (en) * | 2018-11-16 | 2019-03-15 | 浙江大学 | Simulate the device in vitro fertilization and application method in sperm travelling path under natural mode |
-
2015
- 2015-03-26 CN CN201520173107.6U patent/CN204569897U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762192A (en) * | 2015-03-26 | 2015-07-08 | 浙江星博生物科技有限公司 | Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization |
| CN109468226A (en) * | 2018-11-16 | 2019-03-15 | 浙江大学 | Simulate the device in vitro fertilization and application method in sperm travelling path under natural mode |
| CN109468226B (en) * | 2018-11-16 | 2023-08-18 | 浙江大学 | In-vitro fertilization device simulating sperm swimming path in natural state and application method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee after: Chen Binglai Address before: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee before: Zhejiang Cellpro Biotech Co., Ltd. |