CN204569795U - A kind of device preparing collagen fiber membrane - Google Patents
A kind of device preparing collagen fiber membrane Download PDFInfo
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- CN204569795U CN204569795U CN201520148392.6U CN201520148392U CN204569795U CN 204569795 U CN204569795 U CN 204569795U CN 201520148392 U CN201520148392 U CN 201520148392U CN 204569795 U CN204569795 U CN 204569795U
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- sampling receptacle
- volatile alkali
- fiber membrane
- filter screen
- collagen fiber
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Abstract
The utility model provides a kind of device preparing collagen fiber membrane, and described device comprises: sampling receptacle and volatile alkali container, and described sampling receptacle is placed in the top of described volatile alkali container; Wherein, described sampling receptacle is set to for holding collegen filament solution, and the bottom of described sampling receptacle is filter structure.The collegen filament membrane preparation device that the utility model provides, can solve quick, convenient, to prepare collagen fiber membrane efficiently technical problem, and its structure is simple, processing ease, and obtained collagen fiber membrane has high product strength and good security.
Description
Technical field
The utility model relates to medical and health field, and specifically, the utility model relates to a kind of device preparing collagen fiber membrane.
Background technology
Collegen filament have excellent biological safety, and comparatively strong with cellular affinity, also having certain cell inducibility simultaneously, cytodifferentiation can be guided to rise in value, is a kind of prospect tissue engineering bracket material very.Be at present that the film product of raw material mainly contains acellular matrix, collagen protein freeze-drying sponge, collagen electrospinning film etc. with collagen, but they have self inevitable defect.
Acellular matrix: physical strength is good, and there is very reasonably gap structure, excellent environment can be provided for the growth of cell.But acellular matrix is for raw material is obtained by physicochemical method removal cellular constituent by animal skin, pericardium, peritonaeum, little goldbeater's skin etc., this process, not by above-mentioned tissue dispersion, is difficult to likely to cause host that immunoreactive material occurs and removes clean.
Collagen protein freeze-drying sponge: obtained by the collagen fibril solution freeze-drying of extracting from animal tissues.It has excellent biocompatibility, be conducive to the gap structure that cell grows into, but its intensity is lower, limit he as tooth film, hernia paster, the use of meninx etc. can be sewed up.
Collagen electrospinning film: obtained by electrostatic spinning by collagen solution.It has good biocompatibility, preferably mechanical property and certain gap structure, but collagen electrospinning can use the organic solvents such as hexafluoroisopropanol, its toxicity more greatly, not easily Ex-all.There is bibliographical information electrospinning can destroy the triple helix structure of collagen in addition.
Existing collagen fiber membrane complicated process of preparation, operation easier are high, and prepared collagen fiber membrane product strength is low, can not sew up, can not load.Therefore, those skilled in the art are devoted to develop that a kind of structure is simple, the collegen filament membrane preparation device of processing ease.
Utility model content
The purpose of this utility model is to provide a kind of collegen filament membrane preparation device, to solve simple, convenient, to prepare collagen fiber membrane efficiently technical problem, the collagen fiber membrane using collegen filament membrane preparation device of the present utility model to obtain has high product strength.
The utility model provides a kind of device preparing collagen fiber membrane, and described device comprises: sampling receptacle and volatile alkali container, and described sampling receptacle is placed in the top of described volatile alkali container;
Wherein, described sampling receptacle is set to for holding collagen fibril solution, and the bottom of described sampling receptacle is filter structure.
In another preference, described filter screen is selected from: nylon leaching net, stainless steel filtering net.
In another preference, described filter screen is 50-500 object filter screen; More preferably described filter screen is 100-400 object filter screen; Most preferably, described filter screen is 150-250 object filter screen.
In another preference, the sidewall of described sampling receptacle is made up of stainless material.
In another preference, described volatile alkali container is arranged for and holds volatile alkali solution.
In another preference, described volatile alkali is selected from lower group: ammoniacal liquor.
In another preference, described device also comprises housing, and described sampling receptacle and described volatile alkali container are positioned at described enclosure interior.
In another preference, described housing comprises main body and lid, and when described cover cap closes on the body, described main body and described lid form an airtight space, and described sampling receptacle and described volatile alkali container are positioned at described enclosed space.
In another preference, described device also comprises temperature controlling system, and described temperature controlling system is arranged for the temperature of described device inside.
Should be understood that within the scope of the utility model, above-mentioned each technical characteristic of the present utility model and can combining mutually between specifically described each technical characteristic in below (eg embodiment), thus form new or preferred technical scheme.As space is limited, tiredly no longer one by one to state at this.
Accompanying drawing explanation
Fig. 1 is the structural representation of collegen filament membrane preparation device in a preferred implementation of the present utility model.
Fig. 2 is the structural representation of collegen filament membrane preparation device in another preferred implementation of the present utility model.
Embodiment
The collegen filament membrane preparation device that the utility model provides, can solve quick, convenient, to prepare collagen fiber membrane efficiently technical problem, and its structure is simple, processing ease, and obtained collagen fiber membrane has high product strength and good security.
Use device of the present utility model, first collegen filament are dissolved in acidic aqueous solution and obtain collagen fibril sample solution, then volatile alkali is adopted to fumigate, induction collagen fibril forms longer collegen filament, and distribute uniformly, air-dryly obtain hard collagem membrane, the swelling rear freeze-drying of collagem membrane can obtain collagen fiber membrane finished product.Collagen gel after swelling first can be done crosslinking Treatment and then freeze-drying if needed.Adopt the activity that the collagem membrane prepared in this way saves collagen to greatest extent, have good mechanical property and pore texture simultaneously, and non-collagen tissue is easily removed totally, biological safety is excellent.
Compared with traditional collegen filament membrane preparation method, use device of the present utility model to carry out the preparation of collagen fiber membrane, collegen filament length can be retained, most possibly the mechanical strength of reserved materials and biocompatibility.Tradition collegen filament film-forming process, will pulverize collagenous fiber bundle by homogenizer, obtain collegen filament suspension liquid, and then do post-processed.So longer collegen filament have been interrupted, destroy its shaping after mechanical strength, and strong mechanical effect has the risk of collagenous degeneration.
Use device of the present utility model to carry out the preparation of collagen fiber membrane, adopt acid-treated mode to prepare collagen fibril solution, then collagen fibril solution is issued in the inducing action of other ambient conditionss such as alkaline steam and be conigenous assembling, form macrofiber.Fiber solution is air-dry, then swelling, be optionally cross-linked, finally by sample freeze-drying packaging sterilizing or in being directly packaged in containing conserving liquid packaging material and sterilizing.
Can be very easy to according to this technique prepare the collagem membrane with better intensity, its thickness, porosity all can regulate and control according to demand.The product finally obtained can be used for the products such as endocranium sticking patch, tooth film, periosteum, nerve trachea, hernia paster.
The preparation technology of collagen fiber membrane of the present utility model, belongs to biomaterial for medical purpose field, can solve the problem that current collegen filament film toughness and space can not get both, better analogue body extracellular matrix form.Adopt the collagem membrane prepared by method in the utility model can be widely used in the repairing of various soft tissue in body, as endocranium sticking patch, hernia paster, tooth film, skin reparative membrane, neural sticking patch, tendon repairing etc.
A kind of device (as shown in Figure 1) preparing collagen fiber membrane that the utility model provides, described device comprises: sampling receptacle 1 and volatile alkali container 2, and described sampling receptacle 1 is placed in the top of described volatile alkali container 2;
Wherein, described sampling receptacle 1 is set to for holding collagen fibril sample solution 11, and the bottom of described sampling receptacle is filter structure; Described volatile alkali container 2 is arranged for and holds volatile alkali solution 21.
In present embodiment, it is inner that described sampling receptacle 1 can hang on described volatile alkali container 2, and arranging a lid 3, when lid 3 covers on described volatile alkali container 2, forms an airtight space, avoid volatile alkali to leak.
In one preferably embodiment, described device also comprises housing, and described sampling receptacle and described volatile alkali container are positioned at described enclosure interior; Preferably, described housing comprises main body and lid, and when described cover cap closes on the body, described main body and described lid form an airtight space, and described sampling receptacle and described volatile alkali container are positioned at described enclosed space.
In other embodiments, a lid can be set above described sampling receptacle, when above described cover cap is combined in described sampling receptacle, forms an airtight space, avoid volatile alkali to leak.
In one preferably embodiment, using method of the present utility model comprises step:
1. collegen filament are dissolved in acidity (pH0-4.8) aqueous solution, obtain homogeneous collagen fibril solution example.
2. pour in stainless steel frame by sample, stainless steel frame bottom surface 200 order nylon leaching nets are closed.
3., in the encloses container be placed on by sample, bottom encloses container, fill 1% ammoniacal liquor, until sample pH value to 6.0 ± 0.5 time take out, air-dry, obtain collagen fiber membrane.
4. by swelling in the phosphate buffered saline buffer of 0.01M, pH=7.2 for the collagen fiber membrane after air-dry, reach after about 1mm until thickness, take out, clean up with pure water, freeze-drying, can heat cross-linking be carried out if needed.
5. pack after sample cutting, sterilizing.
Major advantage of the present utility model is:
(1) preparation facilities of collagen fiber membrane that provides of the utility model, structure is simple, easy to use.
(2) apply collagen fiber membrane prepared by this device and there is high product strength and good security.
Below in conjunction with specific embodiment, further detailed old the utility model.Should be understood that these embodiments are only not used in restriction scope of the present utility model for illustration of the utility model.Experiment material used in following examples and reagent all can obtain from commercially available channel if no special instructions.
Embodiment 1
As shown in Figure 1, the preparation facilities of the collagen fiber membrane that the present embodiment provides comprises: sampling receptacle 1 and volatile alkali container 2, and described sampling receptacle 1 is placed in the top of described volatile alkali container 2;
Wherein, described sampling receptacle 1 is set to for holding collagen fibril sample solution 11, and the bottom of described sampling receptacle 1 is filter structure; Described volatile alkali container 2 is arranged for and holds volatile alkali solution 21.
The bottom screen structure of sampling receptacle 1, the water in described sample solution can be allowed to flow out described container, and collagen fibril can not be flowed out by described filter structure.Described filter structure can be 50-500 object filter screen; More preferably described filter screen is 100-400 object filter screen; Most preferably, described filter screen is 150-250 object filter screen.
In present embodiment, it is inner that described sampling receptacle 1 can hang on described volatile alkali container 2, and arranging a lid 3, when lid 3 covers on described volatile alkali container 2, forms an airtight space, avoid volatile alkali to leak.
As shown in Figure 2, in one preferably embodiment, described device also comprises temperature controlling system 4, for controlling the temperature in this device.
In one preferably embodiment, described device also comprises housing, and described sampling receptacle and described volatile alkali container are positioned at described enclosure interior; Preferably, described housing comprises main body and lid, and when described cover cap closes on the body, described main body and described lid form an airtight space, and described sampling receptacle and described volatile alkali container are positioned at described enclosed space.
In other embodiments, a lid can be set above described sampling receptacle, when above described cover cap is combined in described sampling receptacle, forms an airtight space, avoid volatile alkali to leak.
The all documents mentioned at the utility model are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the utility model after having read above-mentioned teachings of the present utility model, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. prepare a device for collagen fiber membrane, it is characterized in that, described device comprises: sampling receptacle and volatile alkali container, and described sampling receptacle is placed in the top of described volatile alkali container;
Wherein, described sampling receptacle is set to for holding collagen fibril sample solution, and the bottom of described sampling receptacle is filter structure.
2. device as claimed in claim 1, it is characterized in that, described filter screen is selected from: nylon leaching net and stainless steel filtering net.
3. device as claimed in claim 1, it is characterized in that, described filter screen is 50-500 object filter screen.
4. device as claimed in claim 1, it is characterized in that, described filter screen is 100-400 object filter screen.
5. device as claimed in claim 1, it is characterized in that, the sidewall of described sampling receptacle is made up of stainless material.
6. device as claimed in claim 1, it is characterized in that, described volatile alkali container is arranged for and holds volatile alkali solution.
7. device as claimed in claim 6, it is characterized in that, described volatile alkali is ammoniacal liquor.
8. device as claimed in claim 1, it is characterized in that, described device also comprises housing, and described sampling receptacle and described volatile alkali container are positioned at described enclosure interior.
9. device as claimed in claim 8, it is characterized in that, described housing comprises main body and lid, when described cover cap closes on the body, described main body and described lid form an airtight space, and described sampling receptacle and described volatile alkali container are positioned at described enclosed space.
10. device as claimed in claim 1, it is characterized in that, described device also comprises temperature controlling system, and described temperature controlling system is arranged for the temperature of described device inside.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520148392.6U CN204569795U (en) | 2015-03-16 | 2015-03-16 | A kind of device preparing collagen fiber membrane |
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| CN201520148392.6U CN204569795U (en) | 2015-03-16 | 2015-03-16 | A kind of device preparing collagen fiber membrane |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104695205A (en) * | 2015-03-16 | 2015-06-10 | 苏州景卓生物技术有限公司 | Preparation method of collagen fiber membrane and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104695205A (en) * | 2015-03-16 | 2015-06-10 | 苏州景卓生物技术有限公司 | Preparation method of collagen fiber membrane and application thereof |
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Effective date of registration: 20210415 Address after: No.50-1, Luoxin Road, Baoshan District, Shanghai, 201908 Patentee after: SHANGHAI JINGZE BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: 215421 workshop 4, Xiangtang property, Zhenhui Road, Guizhuang District, Shaxi Town, Taicang City, Suzhou City, Jiangsu Province Patentee before: SUZHOU JINGZHUO BIOTECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |