CN204556504U - Uric acid chemoluminescence method detection kit - Google Patents
Uric acid chemoluminescence method detection kit Download PDFInfo
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- CN204556504U CN204556504U CN201520200686.9U CN201520200686U CN204556504U CN 204556504 U CN204556504 U CN 204556504U CN 201520200686 U CN201520200686 U CN 201520200686U CN 204556504 U CN204556504 U CN 204556504U
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Abstract
The utility model relates to a kind of uric acid chemoluminescence method detection kit, belongs to the use of fluorometric investigation thing.The utility model comprises rectangle box body and the lid with one side edge bending disjunctor, and the notch cuttype vibration-absorbing mounting with most jacks is set in box body, the uricase reagent bottle that cross section is corresponding is held respectively in jack, chemical luminescence for liquid bottle, uric acid calibration solution bottle, high level uric acid quality controlled serum bottle and low value uric acid quality controlled serum bottle.The utility model of such design, chemiluminescence reaction according to the different luminol-peroxidase of application carries out serum uric acid, not only maintain the advantage that sensitivity is higher, the range of linearity is wide, cost is low, accuracy is high, and, be convenient to popularize, can be used for full-automatic mensuration, also can be used for manual mensuration or semi-automatic mensuration, be applicable to large, medium and small medical treatment at different levels and scientific research institution's use.
Description
Technical field
The utility model relates to the use of fluorometric investigation thing, specifically a kind of uric acid chemoluminescence method detection kit.
Background technology
Uric acid is a kind of heterogeneous ring compound containing carbon, nitrogen, oxygen, hydrogen, and its molecular formula is C
5h
4n
4o
3, molecular weight is 168.1103g/mol.In body the growing amount of uric acid and excretion uneven, blood uric acid is caused to raise the disease caused, it is the Etiological of gout, suppressing in the selection that uric acid is high, select the little natural plants of some toxic and side effects to carry out anti-trioxypurine as far as possible, such as deacidification tea etc., because some anti-trioxypurine toxic and side effect is larger, cause 2 injuries to health, under normal circumstances, nearly 1200 milligrams of uric acid in body, every day is newborn into about 600 milligrams, drain 600 milligrams simultaneously, be in the state of balance, if but generation too much has little time excretion or the degeneration of uric acid excretion mechanism in body, then in body, uric acid is detained too much, when blood uric acid concentration is greater than 7 milligrams/liter, cause human body fluid souring, affect the normal function of human body cell, ignore for a long time and will cause gout.In blood, uric acid is all from glomerular filtration, wherein 98% be secreted into again in glomerulus chamber in proximal convoluted tubule stage casing, then 50% re-absorbed uric acid is secreted into again in renal tubule chamber in proximal convoluted tubule stage casing, 40% ~ 44% is had again heavily to be absorbed at proximal convoluted tubule straight section, only have 6% ~ 10% uric acid to discharge, in normal human the generation of uric acid and drainage rate more constant.In body fluid, uric acid content change, fully can reflect the situation of the function such as human body metabolism, immunity.
Clinical labororatory applies different luminol-uricase chemoluminescence method and carries out serum uric acid analysis, and sensitivity is up to 10
-18mol, can detect result fast in 20 minutes, was convenient to realize robotization completely, and the term of validity was up to more than 1 year, and "dead" and teratogen, is convenient to environmental protection and is well received, and was to develop detection technique the most rapidly at present.
Uric acid in serum is under uricase effect, and uric acid is oxidized Hydrogen Peroxide and allantoin, under the catalysis of peroxidase (POD), and H
2o
2generate the different luminol of oxidation and water with different luminol, release photon and luminous, uric acid in serum concentration is higher, and the light signal sent is stronger simultaneously, makes dose-response curve with the uric acid of concentration known and the light signal measured; The uric acid content of unknown sample can from this curve calculating out, and described chemiluminescent substance is different luminol, and the method can be used for measuring uric acid in serum content.
Summary of the invention
The utility model is exactly the problem in order to solve the widespread use of uric acid in serum chemoluminescence method, and provides a kind of uric acid in serum luminol chemiluminescence analysis kit be convenient to medical institutions at different levels and can generally applied.
The utility model is by following Technical Design.
A kind of uricase chemiluminescence assay kit, comprise rectangle box body and the lid with one side edge bending disjunctor, and the notch cuttype vibration-absorbing mounting with most jacks is set in box body, the uricase reagent bottle that cross section is corresponding is held respectively in jack, chemical luminescence for liquid reagent bottle, uric acid calibration solution bottle, high level uric acid quality controlled serum bottle and low value uric acid quality controlled serum bottle.
The utility model of such design not only maintains that sensitivity is higher, the range of linearity is wide, cost is low, accuracy is high, in the reaction, do not need centrifugal or separating step, directly carry out the advantage of luminous intensity mensuration, be convenient to popularize, can be used for full-automatic mensuration, also can be used for manual mensuration or semi-automatic mensuration, be applicable to large, medium and small medical treatment at different levels and scientific research institution's use.
Accompanying drawing explanation
Accompanying drawing is perspective view of the present utility model.
In figure: 1. box body 2. lid
3.4. jack 5. vibration-absorbing mounting
6. uricase reagent bottle 7. chemical luminescence for liquid reagent bottle
8. uric acid calibration solution 9. high level uric acid quality controlled serum bottle
10. low value uric acid quality controlled serum bottle.
Embodiment
A kind of uric acid chemoluminescence method detection kit, comprise rectangle box body 1 and the lid 2 with one side edge bending disjunctor, and the notch cuttype vibration-absorbing mounting 5 with most jacks 3,4 is set in box body 1, the uricase reagent bottle 6 that cross section is corresponding is held respectively in jack 3,4, chemical luminescence for liquid reagent kit bottle 7, uric acid calibration solution bottle 8, high level uric acid quality controlled serum bottle 9 and low value uric acid quality controlled serum bottle 10.
Described uric acid chemoluminescence method detection kit, 3 jack 3 cross sections on its notch cuttype vibration-absorbing mounting 5 are squares, hold the uric acid calibration solution bottle 8 of round section respectively, high level uric acid quality controlled serum bottle 9 and low value uric acid quality controlled serum bottle 10.
Described uric acid in serum chemical luminescence reagent kit, is characterized in that: 4 jack 4 cross sections under notch cuttype vibration-absorbing mounting 5 are isosceles trapezoids, holds 2 uricase reagent bottles 6 and 2 chemical luminescence for liquid reagent bottles 7 in corresponding isosceles trapezoid cross section respectively.And the isosceles trapezoid jack 4 on identical base is gap-forming, i.e. the inverted isosceles trapezoid jack 4 of gap-forming, one in the corresponding reagent bottle 6 or 7 in therefore 2 isosceles trapezoid cross sections be 180 ° rotate after settle.
Described uric acid in serum chemical luminescence reagent kit, its notch cuttype vibration-absorbing mounting 5 with jack 3,4 is that styrofoam is shaping.
Described uric acid in serum chemical luminescence reagent kit, its notch cuttype vibration-absorbing mounting 5 with jack 3,4 is cardboard cut hole bending formings.Notch cuttype vibration-absorbing mounting 5 plays the support to reagent bottle, fixing and cushioning effect.
50ml uricase mixed liquor is preinstalled with respectively in described uric acid chemical illuminating reagent bottle 6,50ml different luminol application liquid is pre-installed respectively in chemical luminescence for liquid bottle 7,2ml uric acid calibration solution is preinstalled with in uric acid calibration solution bottle 8, be preinstalled with 2ml high level uric acid quality controlled serum in high level uric acid quality controlled serum bottle 9, and be preinstalled with 2ml low value uric acid quality controlled serum in low value uric acid quality controlled serum bottle 10.
Reagent forms:
Uricase reagent: phosphate buffer 0.4mmol/L pH=7.5, peroxidase (POD) >1.5U/mL, uricase (GOD) >15u/mL.Also the commercial goods of identical component can be adopted.
Chemical luminescence for liquid: 100ml Tris buffer: containing different luminol: 0.01mol/L 0.178g, Trisbuffer fill a prescription: 0.1mol/L 6.07g Tris is dissolved in 500ml water, adjusts pH 8.8 with hydrochloric acid.Also the commercial goods of identical component can be adopted.
Described uric acid calibration solution, high level uric acid quality controlled serum and low value uric acid quality controlled serum are commercial goods.
Instrument: MPC-1 single photon chemical illumination immunity analysis instrument.
Assay method: the ratio of Dai test sample Pin ﹕ uricase Shi Ji ﹕ chemical luminescence for liquid is 1:50:50.Temperature 37 DEG C temperature bath 5min, measures luminous intensity RLU value, and with the calibration of uric acid calibration solution, during mensuration testing sample and high and low control liquid Simultaneously test, when high and low control liquid measured value is all in this scope, its determination data is reliable.
Make dose-response curve, luminous intensity RLU value per sample obtains the concentration of serum uric acid from this curve, or is directly obtained by formula:
Concentration=(sample RLU value/standard RLU value) × normal concentration of uric acid.
Said method sensitivity is higher, the range of linearity is wide, and monitoring range is 2.0 × 10
-7~ 6.0 × 10
-3mmol/L, cost is low, accuracy is high, the light signal of mensuration, can photon counting one by one, and therefore preci-sion and accuracy is higher than colourimetry; In the reaction, do not need centrifugal or separating step, directly carry out luminous intensity mensuration.
Claims (5)
1. a uric acid chemoluminescence method detection kit, comprise rectangle box body (1) and the lid (2) with one side edge bending disjunctor, it is characterized in that: the notch cuttype vibration-absorbing mounting (5) with most jacks (3,4) is set in box body (1), the uricase reagent bottle (6) that cross section is corresponding is held respectively in jack (3), (4), chemical luminescence for liquid reagent bottle (7), uric acid calibration solution bottle (8) and high level uric acid quality controlled serum bottle (9) and low value uric acid quality controlled serum bottle (10).
2. uric acid chemoluminescence method detection kit according to claim 1, it is characterized in that: 3 jack (3) cross sections on notch cuttype vibration-absorbing mounting (5) are squares, hold the uric acid calibration solution bottle (8) of square sectional respectively, high level uric acid quality controlled serum bottle (9) and low value uric acid quality controlled serum bottle (10).
3. uric acid chemoluminescence method detection kit according to claim 1, it is characterized in that: 4 jack (4) cross sections under notch cuttype vibration-absorbing mounting (5) are isosceles trapezoids, hold 2 uricase reagent bottles (6) and 2 chemical luminescence for liquid reagent bottles (7) in isosceles trapezoid cross section respectively.
4. uric acid chemoluminescence method detection kit according to claim 1, is characterized in that: the notch cuttype vibration-absorbing mounting (5) with jack (3,4) is that styrofoam is shaping.
5. uric acid chemoluminescence method detection kit according to claim 1, is characterized in that: the notch cuttype vibration-absorbing mounting (5) with jack (3,4) is bending forming after cardboard cut hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520200686.9U CN204556504U (en) | 2015-04-06 | 2015-04-06 | Uric acid chemoluminescence method detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520200686.9U CN204556504U (en) | 2015-04-06 | 2015-04-06 | Uric acid chemoluminescence method detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204556504U true CN204556504U (en) | 2015-08-12 |
Family
ID=53831598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520200686.9U Expired - Fee Related CN204556504U (en) | 2015-04-06 | 2015-04-06 | Uric acid chemoluminescence method detection kit |
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| Country | Link |
|---|---|
| CN (1) | CN204556504U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020115119A (en) * | 2019-01-17 | 2020-07-30 | 大韓民国（国立水産科学院）ＲＥＰＵＢＬＩＣ ＯＦ ＫＯＲＥＡ（Ｎａｔｉｏｎａｌ Ｉｎｓｔｉｔｕｔｅ ｏｆ Ｆｉｓｈｅｒｉｅｓ Ｓｃｉｅｎｃｅ） | Environment stress inducer measuring device |
| CN113655045A (en) * | 2021-09-27 | 2021-11-16 | 天津中医药大学 | Uric acid detection method and application thereof |
-
2015
- 2015-04-06 CN CN201520200686.9U patent/CN204556504U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020115119A (en) * | 2019-01-17 | 2020-07-30 | 大韓民国（国立水産科学院）ＲＥＰＵＢＬＩＣ ＯＦ ＫＯＲＥＡ（Ｎａｔｉｏｎａｌ Ｉｎｓｔｉｔｕｔｅ ｏｆ Ｆｉｓｈｅｒｉｅｓ Ｓｃｉｅｎｃｅ） | Environment stress inducer measuring device |
| CN113655045A (en) * | 2021-09-27 | 2021-11-16 | 天津中医药大学 | Uric acid detection method and application thereof |
| CN113655045B (en) * | 2021-09-27 | 2023-12-26 | 天津中医药大学 | Uric acid detection method and application thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150812 Termination date: 20160406 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |