CN204500712U - A kind of modified model Brachydanio rerio auditory function detects water tank - Google Patents
A kind of modified model Brachydanio rerio auditory function detects water tank Download PDFInfo
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- CN204500712U CN204500712U CN201520131119.2U CN201520131119U CN204500712U CN 204500712 U CN204500712 U CN 204500712U CN 201520131119 U CN201520131119 U CN 201520131119U CN 204500712 U CN204500712 U CN 204500712U
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Abstract
This utility model relates to a kind of modified model Brachydanio rerio auditory function and detects water tank, comprise tank parts and holder part, tank parts is arranged on holder part upper end, tank parts comprises loudspeaker water tank (6) and audiometry water tank (12), the baffle plate (3) that loudspeaker water tank (6) is adjacent with audiometry water tank (12) has transaudient hole slot, loudspeaker water tank (6) is built-in with loudspeaker (4) under water, Brachydanio rerio placing rack (11) is installed in audiometry water tank (12), Brachydanio rerio placing rack (11) is provided with the Brachydanio rerio fixture (10) for fixing Brachydanio rerio, loudspeaker water tank (6), the equal ground connection in surface of audiometry water tank (12), this utility model compared with the existing technology, novel structure, simple, the not only perfect experiment condition giving sound, Underwater Recording under water, achieves the hearing test of Brachydanio rerio multiple frequencies under real conditions, significantly reduce electromagnetic interference, substantially increase the accuracy of audiology's research.
Description
[technical field]
This utility model relates to Brachydanio rerio hearing test technical field, and specifically a kind of modified model Brachydanio rerio auditory function detects water tank.
[background technology]
Brachydanio rerio is important model organism, for the research of the growth of multiple systems, function and disease.Brachydanio rerio as experimental animal model, for the researchs such as the reconstruction after the growth of auditory function, hearing loss provide a great convenience.And the hearing test of the Brachydanio rerio large obstacle that is always one in this kind of research.In early stage research, the hearing test of Brachydanio rerio completes in atmosphere, is all arranged in air namely to the loudspeaker of sound and tested fish.Although this kind of measuring technology is ripe, but there is the shortcoming that can not be ignored: on the one hand, detected fish needs to breathe holdout device; On the other hand, detected fish has departed from state orthobiosis.Therefore, the hearing test of the Brachydanio rerio completed in atmosphere can not reflect actual auditory function exactly.
Although in nearest research, Brachydanio rerio hearing test has achieved and has completed under water, relevant report show under water in test process electromagnetic interference large, had a strong impact on the interpretation of test result.Thus, if the platform of a kind of jamproof Brachydanio rerio hearing test under water can be provided, audiology's research of Fish will be a significant benefit to.
[utility model content]
The purpose of this utility model is exactly will solve above-mentioned deficiency and provide a kind of modified model Brachydanio rerio auditory function to detect water tank, the perfect experiment condition giving sound, Underwater Recording under water, achieve the hearing test of Brachydanio rerio multiple frequencies under real conditions, significantly reduce electromagnetic interference, substantially increase the accuracy of audiology's research.
Design a kind of modified model Brachydanio rerio auditory function for achieving the above object and detect water tank, comprise tank parts and holder part, described tank parts is arranged on holder part upper end, described tank parts comprises loudspeaker water tank 6 and audiometry water tank 12, the baffle plate 3 that described loudspeaker water tank 6 is adjacent with audiometry water tank 12 has transaudient hole slot, described loudspeaker water tank 6 is built-in with loudspeaker 4 under water, in described audiometry water tank 12, Brachydanio rerio placing rack 11 is installed, described Brachydanio rerio placing rack 11 is provided with the Brachydanio rerio fixture 10 for fixing Brachydanio rerio, described loudspeaker water tank 6, the equal ground connection in surface of audiometry water tank 12.
Described Brachydanio rerio fixture 10 comprises overarm brace 13, link 15 and collet 16, described link 15 one end is fixedly connected with overarm brace 13, described link 15 other end is fixedly connected with collet 16, described overarm brace 13 is connected on Brachydanio rerio placing rack 11, described overarm brace 13 is provided with electrode jack 17, is provided with Electrode connection screw 14 in described electrode jack 17.
Described loudspeaker water tank 6 inside bottom surface is provided with a slot 5, and described loudspeaker under water 4 are fixed in loudspeaker water tank 6 by slot 5.
The end face of described loudspeaker water tank 6 is provided with case lid 2, the bottom surface of described case lid 2 and loudspeaker water tank 6, leading flank, trailing flank, left surface all adopt stainless steel to make, and the baffle plate 3 that described loudspeaker water tank 6 is adjacent with audiometry water tank 12 adopts lucite-copper coin to make.
Survey wall in described audiometry water tank 12 and be provided with symmetrical level trough 18, described Brachydanio rerio placing rack 11 packaged type is placed in level trough 18.
The bottom of described loudspeaker water tank 6, audiometry water tank 12 is equipped with outlet valve ground connection mouth 7, described loudspeaker water tank 6 and audiometry water tank 12 is equipped with the socket of earth cable.
Described holder part comprises support 9 and is connected to the rustless steel bound edge 8 of support 9 upper end, described rustless steel bound edge 8 is provided with the draw-in groove matched with tank parts bottom, described tank parts and holder part are connected as one by draw-in groove, and described support 9 adopts stainless steel to make.
Described audiometry water tank 12, Brachydanio rerio placing rack 11, Brachydanio rerio fixture 10 all adopt lucite to make.
This utility model compared with the existing technology, novel structure, simply, reasonable in design, by by loudspeaker water tank, audiometry water tank is placed on support, the baffle plate making loudspeaker water tank adjacent with audiometry water tank has transaudient hole slot, and at the built-in loudspeaker under water of loudspeaker water tank, the Brachydanio rerio placing rack and Brachydanio rerio fixture that are used for fixing Brachydanio rerio and test electrode are installed in audiometry water tank, this loudspeaker water tank, the equal ground connection in surface of audiometry water tank, to conduct the electrostatic on water tank surface, thus perfect give sound under water, the experiment condition of Underwater Recording, achieve the hearing test of Brachydanio rerio multiple frequencies under real conditions, significantly reduce electromagnetic interference, substantially increase the accuracy of audiology's research, and equipment operating is easy, does not need extra Brachydanio rerio to breathe holdout device, be conducive to large-scale promotion, in addition, utilize this auditory function to detect water tank, the real-time monitoring to underwater noise can also be realized, thus carry out the correlational study of Brachydanio rerio noise-induced auditory function damage, perfect further Brachydanio rerio auditory function research platform.
[accompanying drawing explanation]
Fig. 1 is STRUCTURE DECOMPOSITION figure of the present utility model;
Fig. 2 is perspective view of the present utility model;
Fig. 3 is the STRUCTURE DECOMPOSITION figure of Brachydanio rerio fixture in this utility model;
Fig. 4 is the perspective view of Brachydanio rerio fixture in this utility model;
Fig. 5 is fundamental diagram of the present utility model;
Fig. 6 is the Brachydanio rerio ABR test waveform figure utilizing this utility model detection water tank to carry out;
Fig. 7 is the Brachydanio rerio ABR test waveform figure utilizing existing Brachydanio rerio auditory function detection water tank to complete;
In figure: 1, rustless steel dialectal switching 2, case lid 3, baffle plate 4, under water loudspeaker 5, slot 6, loudspeaker water tank 7, outlet valve ground connection mouth 8, rustless steel bound edge 9, support 10, Brachydanio rerio fixture 11, Brachydanio rerio placing rack 12, audiometry water tank 13, overarm brace 14, Electrode connection screw 15, link 16, collet 17, electrode jack 18, level trough.
[detailed description of the invention]
Below in conjunction with accompanying drawing, following further illustrating is done to this utility model:
As shown in drawings, this utility model comprises: tank parts and holder part, tank parts is arranged on holder part upper end, tank parts comprises loudspeaker water tank 6 and audiometry water tank 12, the baffle plate 3 that loudspeaker water tank 6 is adjacent with audiometry water tank 12 has transaudient hole slot, loudspeaker water tank 6 is built-in with loudspeaker 4 under water, Brachydanio rerio placing rack 11 is installed in audiometry water tank 12, Brachydanio rerio placing rack 11 is provided with the Brachydanio rerio fixture 10 for fixing Brachydanio rerio, the equal ground connection in surface of loudspeaker water tank 6, audiometry water tank 12; Wherein, Brachydanio rerio fixture 10 comprises overarm brace 13, link 15 and collet 16, link 15 one end is fixedly connected with overarm brace 13, link 15 other end is fixedly connected with collet 16, overarm brace 13 is connected on Brachydanio rerio placing rack 11, overarm brace 13 is provided with electrode jack 17, is provided with Electrode connection screw 14 in electrode jack 17.
In this utility model, loudspeaker water tank 6 inside bottom surface is provided with a slot 5, and loudspeaker 4 are fixed in loudspeaker water tank 6 by slot 5 under water.The end face of loudspeaker water tank 6 is provided with case lid 2, and the bottom surface of case lid 2 and loudspeaker water tank 6, leading flank, trailing flank, left surface all adopt stainless steel to make, and the baffle plate 3 that loudspeaker water tank 6 is adjacent with audiometry water tank 12 adopts lucite-copper coin to make; Survey wall in audiometry water tank 12 and be provided with symmetrical level trough 18, Brachydanio rerio placing rack 11 packaged type is placed in level trough 18.The bottom of loudspeaker water tank 6, audiometry water tank 12 is equipped with outlet valve ground connection mouth 7, loudspeaker water tank 6 and audiometry water tank 12 is equipped with the socket of earth cable.Audiometry water tank 12, Brachydanio rerio placing rack 11, Brachydanio rerio fixture 10 all adopt lucite to make.Holder part comprises support 9 and is connected to the rustless steel bound edge 8 of support 9 upper end, and rustless steel bound edge 8 is provided with the draw-in groove matched with tank parts bottom, and tank parts and holder part are connected as one by draw-in groove, and support 9 adopts stainless steel to make.
This utility model improves the accuracy of test Brachydanio rerio hearing test, achieves environment under water and completes the test of Brachydanio rerio auditory function in high quality.This anti-interference Brachydanio rerio auditory function detects water tank and is made up of tank parts and holder part.The main material of tank parts is lucite, and overall dimensions is long 100* wide 42* height 50cm, and tank parts is divided into two bins: loudspeaker water tank and audiometry water tank.Loudspeaker water tank is for placing loudspeaker under water, be of a size of long 20* wide 42* height 50cm, the material of top, the end and three sides is rustless steel, and the side material adjacent with audiometry water tank is copper, thickness is 0.2cm, and there is the slot of fixing loudspeaker under water the bottom surface inside loudspeaker water tank.Audiometry water tank is for placing Brachydanio rerio, be of a size of long 80* wide 42* height 50cm, survey long axis direction 27cm At The Height both sides in audiometry water tank and respectively have a level trough, built-in two supports of level trough, can move along major axis, the material of level trough and support is lucite, for placing fixing Brachydanio rerio and fixture thereof, makes it hang under water.The material of Brachydanio rerio fixture is lucite, for Brachydanio rerio and the test electrode of fixing test.This device is made up of collet, link and overarm brace.Collet is of a size of long 4* wide 3* height 0.4cm, and link is of a size of long 4* wide 0.4* height 3.5cm, and overarm brace is of a size of long 10* wide 3.5* height 2.2cm.Separated by lucite-copper coin between loudspeaker water tank and audiometry water tank, plate has the hole slot of logical sound.Loudspeaker water tank and audiometry water tank have the socket of earth cable, for conducting the electrostatic on water tank surface.Holder part material is rustless steel, and be of a size of long 100* wide 42* height 40cm, for supporting and placing tank parts, holder part is connected with draw-in groove with tank parts.
As shown in Figure 5, operation principle of the present utility model is: the loudspeaker under water in loudspeaker water tank are to sound, and sound arrives audiometry water tank by transaudient hole slot, and realize the Sound stimulat to Brachydanio rerio, water tank surface earthing realizes anti-interference.
As shown in accompanying drawing 6 and accompanying drawing 7, be the oscillogram that the Brachydanio rerio ABR utilizing different audiometry water tank to carry out tests, wherein, Fig. 6 is the test utilizing this utility model detection water tank to complete, and its waveform is clear, and clutter is few; And Fig. 7 is the test utilizing existing Brachydanio rerio auditory function detection water tank to complete, its main ripple is not easy to identify, and clutter is many.And, through overtesting, utilize anti-interference Brachydanio rerio auditory function described in the utility model detect water tank and under water loudspeaker can produce stable high intensity noise, in order to the research of Brachydanio rerio noise-induced acoustic trauma.
The Brachydanio rerio auditory function series of studies that utilized this utility model to carry out, detects listening property of Brachydanio rerio ABR (ABR) threshold value of different developmental phases (12mm-46mm).Be reported as Brachydanio rerio adult fish audible frequency range is 100-4000Hz in the past, and in growth course, threshold value does not have significant change, but there is audible frequency range expansion (expanding to 4000Hz from the 200Hz of juvenile fish).And detect water tank by anti-interference Brachydanio rerio auditory function described in the utility model, confirm that all Brachydanio rerio hearing frequencies are 100-12000Hz, without listening expansion frequently; ABR threshold value satellite length increases and changes, and 31-36.5mm group audition ABR threshold value in all frequencies is minimum, and best audible frequency range is 600-1000Hz.This conclusion has expanded the understanding to the development of Brachydanio rerio auditory function greatly.
This utility model is not by the restriction of above-mentioned embodiment; change, the modification done under other any does not deviate from spirit of the present utility model and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection domain of the present utility model.
Claims (8)
1. a modified model Brachydanio rerio auditory function detects water tank, it is characterized in that: comprise tank parts and holder part, described tank parts is arranged on holder part upper end, described tank parts comprises loudspeaker water tank (6) and audiometry water tank (12), the baffle plate (3) that described loudspeaker water tank (6) is adjacent with audiometry water tank (12) has transaudient hole slot, described loudspeaker water tank (6) is built-in with loudspeaker (4) under water, in described audiometry water tank (12), Brachydanio rerio placing rack (11) is installed, described Brachydanio rerio placing rack (11) is provided with the Brachydanio rerio fixture (10) for fixing Brachydanio rerio, described loudspeaker water tank (6), the equal ground connection in surface of audiometry water tank (12).
2. modified model Brachydanio rerio auditory function as claimed in claim 1 detects water tank, it is characterized in that: described Brachydanio rerio fixture (10) comprises overarm brace (13), link (15) and collet (16), described link (15) one end is fixedly connected with overarm brace (13), described link (15) other end is fixedly connected with collet (16), described overarm brace (13) is connected on Brachydanio rerio placing rack (11), described overarm brace (13) is provided with electrode jack (17), Electrode connection screw (14) is provided with in described electrode jack (17).
3. modified model Brachydanio rerio auditory function as claimed in claim 1 or 2 detects water tank, it is characterized in that: described loudspeaker water tank (6) inside bottom surface is provided with a slot (5), and described loudspeaker under water (4) are fixed in loudspeaker water tank (6) by slot (5).
4. modified model Brachydanio rerio auditory function as claimed in claim 3 detects water tank, it is characterized in that: the end face of described loudspeaker water tank (6) is provided with case lid (2), the bottom surface of described case lid (2) and loudspeaker water tank (6), leading flank, trailing flank, left surface all adopt stainless steel to make, and described loudspeaker water tank (6) baffle plate (3) adjacent with audiometry water tank (12) adopts lucite-copper coin to make.
5. modified model Brachydanio rerio auditory function as claimed in claim 4 detects water tank, it is characterized in that: survey wall in described audiometry water tank (12) and be provided with symmetrical level trough (18), described Brachydanio rerio placing rack (11) packaged type is placed in level trough (18).
6. modified model Brachydanio rerio auditory function as claimed in claim 5 detects water tank, it is characterized in that: the bottom of described loudspeaker water tank (6), audiometry water tank (12) is equipped with outlet valve ground connection mouth (7), described loudspeaker water tank (6) and audiometry water tank (12) are equipped with the socket of earth cable.
7. modified model Brachydanio rerio auditory function as claimed in claim 6 detects water tank, it is characterized in that: described holder part comprises support (9) and is connected to the rustless steel bound edge (8) of support (9) upper end, described rustless steel bound edge (8) is provided with the draw-in groove matched with tank parts bottom, described tank parts and holder part are connected as one by draw-in groove, and described support (9) adopts stainless steel to make.
8. modified model Brachydanio rerio auditory function as claimed in claim 7 detects water tank, it is characterized in that: described audiometry water tank (12), Brachydanio rerio placing rack (11), Brachydanio rerio fixture (10) all adopt lucite to make.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757978A (en) * | 2015-03-06 | 2015-07-08 | 上海市第六人民医院 | Anti-interference zebra fish hearing function detection water tank |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757978A (en) * | 2015-03-06 | 2015-07-08 | 上海市第六人民医院 | Anti-interference zebra fish hearing function detection water tank |
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