CN204405612U - A kind of liquid chromatograph - Google Patents
A kind of liquid chromatograph Download PDFInfo
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- CN204405612U CN204405612U CN201520096836.6U CN201520096836U CN204405612U CN 204405612 U CN204405612 U CN 204405612U CN 201520096836 U CN201520096836 U CN 201520096836U CN 204405612 U CN204405612 U CN 204405612U
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Abstract
The utility model provides a kind of liquid chromatograph, this liquid chromatograph comprises the first flow (L3) being connected to the first chromatographic column (C1), second runner (L13), analyze runner (L14), waste liquid runner (L4), middle chromatographic column (C2), multiple flow passages transfer valve (V1), deposit valve (V2) and solution replacing valve (V3), correct transfer valve (V4) etc., first mobile phase and the second flowing separate by introducing gas by this liquid chromatograph, both are avoided mixing to impact, the first mobile phase in middle chromatographic column is replaced again by the second mobile phase, the impurity avoiding the first flowing facies tract to enter produces matrix effect on the detector, can also before the second mobile phase replaces the first mobile phase in middle chromatographic column, increase chromatographic column function in the middle of the prewashing of modulation solution, the impact of further minimizing matrix, further in analysis runner, increase calibration solution, thus the impact that the matrix effect of calibrated detector is analyzed target components.
Description
Technical field
The utility model belongs to liquid chromatography field, is specifically related to a kind of liquid chromatograph with solvent replacement function.
Background technology
Detecting device is used for being detected by the material that chromatographic column is separated in liquid-phase chromatographic analysis, and the multiple detecting devices such as existing mass spectrum, fluorescence, galvanochemistry are available, liquid chromatography and various detection coupling, reaches high sensitivity and is separated the object detected.Especially the coupling (liquid Chromatograph Mass Spectrometer is called for short LC-MS) of liquid chromatography and Mass Spectrometer Method, the effective means of Chang Zuowei detection of complex organic mixture.It is using liquid chromatography as piece-rate system, and mass spectrum is detection system.Sample is separated with mobile phase in mass spectrum part, and after being ionized, separated by mass number by fragment ion through mass spectrographic mass analyzer, device obtains mass spectrogram after testing.LC-MS, by selectivity, high sensitivity and the advantages that can provide relative molecular mass and structural information, makes chromatogram and mass spectrum have complementary advantages, is widely used in many fields such as Pharmaceutical Analysis, food analysis and environmental analysis.
Along with the continuous application of LC-MS, find that the matrix of sample and the matrix of mobile phase have a significant effect to the detection sensitivity of dissimilar detecting device and stability, be called " matrix effect ", matrix effect refers to that other matrix components in sample except determinand are on the impact of determinand measured value, when changing kind and the concentration of sample substrate, determinand response on the detector can reduce or increase.Matrix components is derived from the material that flows out altogether with measured object in chromatographic separation process to the impact of measured object ionization process, and the matrix components of main report has polymer residue, phthalate, detergent catabolite, ion-pairing agent, organic acid plasma to exchange promoter and chromatographic column fixed phase h substance etc. at present.The existence of matrix effect can have a strong impact on quantitative precision to determinand and precision, and influence factor is changeable, is difficult to eliminate.In addition during the coupling of the high sensitivity detector such as liquid chromatography and fluorescence, mobile phase intermediate ion, organism, solvent also can impact detecting device.
(2D-LC) that developed in recent years, frequent and mass detector conbined usage, is purified by the multistage chromatography of Two-dimensional Liquid Chromatography, reaches the object reducing matrix effect.Two-dimensional liquid chromatography is the piece-rate system getting up to form by two different and separate for separating mechanism Coupled columns.Sample enters in interface through the chromatographic column of the first dimension liquid phase, by concentrating, trap or being switched in the chromatographic column and detecting device entering the second dimension liquid phase after cutting, is then detected by rear end mass detector by the material of the second dimension chromatographic resolution.Such first dimension liquid phase removes most of impurity in sample, and the salt or the sample substrate that therefore enter two-dimensional liquid chromatography are little, thus can reduce " matrix effect " that sample and the first dimension mobile phase mesostroma cause mass spectrum.
But there is such problem in above technical scheme: sample need be forced through the first dimension chromatographic column by the mobile phase of the first dimension liquid chromatography, comprise the interface of the first dimension mobile phase by two-dimensional liquid chromatography of sample object component, will enter in the chromatographic column of the second dimension liquid phase, therefore when containing high salt concentration (such as phosphate in the first dimension liquid phase mobile phase, acetate) or other improve the modifying agent of chromatographic resolution time (such as triethylamine, triethylammonium tetrakis, sodium heptanesulfonate etc.), these salt or modifier also can enter rear end mass detector, thus cause obvious matrix effect, especially when first dimension mobile phase and second tie up mobile phase become phase-splitting difference larger time, the matrix effect produced is more obvious, thus have a strong impact on quantitative precision and the precision of determinand, therefore the matrix effect that the matrix that how the first dimension mobile phase brings in online elimination Two-dimensional Liquid Chromatography produces at the second dimension liquid chromatographic system is extremely important.
Utility model content
For the deficiencies in the prior art, the utility model is intended to the problem solving liquid-phase chromatographic analysis mesostroma effect, following three objects of main realization:
One of the purpose of this utility model: utilize the second dimension mobile phase to remove the first mobile phase bringing the second dimension chromatographic system into online, avoid the matrix effect that the first mobile phase causes.
The purpose of this utility model two: use assisted solution, bears the prerinse of the middle chromatographic column of target acquisition component, avoids matrix effect further in cleaning Two way chromatograms.
The purpose of this utility model three: import calibration solution online in two-dimensional liquid chromatography system, sequential or simultaneously by detecting device with detected object, thus correct the size that matrix effect produces, reach the object eliminating matrix effect impact further.
For achieving the above object, the technical solution of the utility model is:
One of technical scheme:
A kind of liquid chromatograph, comprising: the first flow being connected with the first chromatographic column, for carrying the first mobile phase, and carrying out initial gross separation to sample; Second runner, for carrying the second mobile phase; Be connected with the analysis runner of the second chromatographic column, for carrying out separation and detection to the material of catching; First waste liquid runner, for getting rid of waste liquid; Also comprise be provided with multiple interface multiple flow passages transfer valve, be provided with multiple port deposit valve, described in deposit valve any two ends mouth between be connected with middle chromatographic column; And be provided with the solution replacing valve of multiple port, be connected with atmosphere storage ring between any two ends mouth of described solution replacing valve;
Any three interfaces of described multiple flow passages transfer valve are connected with one end of the first connecting line, the second connecting line and the 3rd connecting line respectively, and described first connecting line is communicated with two ports of solution replacing valve respectively with the other end of the second connecting line; The other end of described 3rd connecting line is not communicated with any two idle ports of a port and solution replacing valve of depositing valve by many reduction of fractions to a common denominators; Described first flow, the second runner, analysis runner and waste liquid runner are connected in all the other any one idle interface on multiple flow passages transfer valve; Describedly deposit the 4th connecting line be provided with between valve with solution replacing valve for both being directly connected; All the other idle ports of described solution replacing valve are also connected to gas input flow road, gas exhaust manifold and the second waste liquid and discharge runner.
Preferred connected mode: described multiple flow passages transfer valve comprises interface a, interface b, interface c, interface d, interface e, interface f, interface g and interface j; Described interface a is connected with first flow, described interface b is connected with waste liquid runner, described interface c is connected with the first connecting line, described interface d is connected with analysis runner, described interface e is connected with the second runner, described interface f is connected with the second connecting line, and described interface j is connected with the 3rd connecting line.
Described multiple flow passages transfer valve can also comprise interface h and interface i, and described interface h and interface i is sealed condition.
Preferred connected mode: described in deposit valve and comprise port a, port b, port c, port d, port e, port f; Be provided with middle chromatographic column (C2) between described port a and port d, described port e is connected with the 3rd connecting line, and described port f is connected with the 4th connecting line.Described port b and port c is sealed condition.
Preferred connected mode: described solution replacing valve comprises port g, port h, port i, port j, port k, port l, port m, port n, port o, port p; Be provided with atmosphere storage ring between described port m and port j, port l be connected with gas input flow road, port k is connected with gas exhaust manifold; All the other ports are connected to the first connecting line, the second connecting line, the 4th connecting line, the second waste liquid discharge runner, and the two articles of branch lines be connected with the 3rd connecting line.
Described two branch lines are preferably equipped with retaining valve.
This liquid chromatograph, by selecting the conducting direction of solution replacing valve port, promotes gas by gas storage ring with air pump or gases at high pressure; By selecting solution replacing valve another location, select multiple flow passages transfer valve and the position of depositing valve simultaneously, second flow paths downstream, gas storage ring, middle chromatographic column are communicated, solution now in chromatographic column and the second runner solution are separated by gas in gas storage ring, avoid the mutually miscible of two kinds of solution, second runner solution is under infusion pump promotes, in middle chromatographic column, original solution is first released by the gas in gas storage ring, then the second runner solution enters middle chromatographic column, has kept away the replacement of solution in middle chromatographic column.
Technical scheme two:
A kind of liquid chromatograph, on the basis of technical scheme one, also comprise modulation runner, for carrying modulation solution, described modulation runner is connected in an idle interface of multiple flow passages transfer valve, and this idle interface is adjacent with the interface being connected with the second connecting line.Preferably, modulate runner to be connected on the interface g of multiple flow passages transfer valve.
By cleaning solvent (being generally the organic solvent of water or low concentration), prewashing is carried out to the material be captured in middle chromatographic column, remove water-soluble larger material, then carry out solution replacement, the impact of matrix can be eliminated further.
Technical scheme three:
A kind of liquid chromatograph, on the basis of technical scheme one, also comprise the correction transfer valve being provided with multiple port, two ports of described correction transfer valve are connected on described analysis runner, and are positioned at the front-end position of the second chromatographic column; Be connected with calibration solution storage ring between any two idle ports of described correction transfer valve, all the other idle ports are also connected to calibration solution runner and the 3rd waste liquid runner.
Preferred connected mode is: described correction transfer valve (V4) comprises port q, port r, port s, port t, port u, port v; Be connected with calibration solution storage rings between described port s and port v, described port q and port r is connected to and analyzes on runner, and described port t, port u are connected with calibration solution runner and the 3rd waste liquid runner respectively.
Further preferred, described middle chromatographic column chooses the Stationary liquid that reserve capability is better than the first chromatographic column reserve capability.
Calibration solution is imported by correcting transfer valve in analysis runner, sequential or simultaneously by detecting device with detected object, carry out the correction that matrix affect detecting device, reach the object eliminated matrix effect and affect further.
Technical scheme two and technical scheme three separate, can add respectively in technical scheme one, also can add in technical scheme one simultaneously.
Except special instruction, multiple flow passages transfer valve described in the utility model, deposit valve, solution replacing valve and correct the general port of transfer valve or interface and be only connected with a pipeline.Multiple flow passages transfer valve described in the utility model, deposit valve all and to correct transfer valve be two transfer valves.
Except the port being in closed state or interface, the port of connecting line or interface is not had to be called idle port or idle interface.
Compared with prior art, advantage of the present utility model is: adopt the second mobile phase to remove the matrix of the first flowing facies tract online, and structure is simple, and does not introduce other solvents, reduces Operating Complexity; Introduce gas by atmosphere storage ring, the first mobile phase and the second mobile phase are thoroughly separated, the scavenging period avoiding the mutual mixing of two solution to cause extends; Matrix compatible in further employing cleaning solution cleaning intermediolateral column, the matrix that the first dimension mobile phase is brought in the second dimension mobile phase is less, reduces the better effects if of " matrix effect "; Further increase calibration solution, revise the impact of matrix on target components by the change on the detector of the material in calibration solution, further reduce " matrix effect " to the impact of testing result.
Accompanying drawing explanation
Fig. 1 is the liquid chromatograph structural representation of embodiment 1;
Fig. 2 is a kind of working state figure of the liquid chromatograph of embodiment 1;
Fig. 3 is a kind of working state figure of the liquid chromatograph of embodiment 1;
Fig. 4 is a kind of working state figure of the liquid chromatograph of embodiment 1;
Fig. 5 is a kind of working state figure of the liquid chromatograph of embodiment 1;
Fig. 6 is a kind of working state figure of the liquid chromatograph of embodiment 1;
Fig. 7 is the structural representation of the liquid chromatograph of embodiment 2;
Fig. 8 is a kind of working state figure of the liquid chromatograph of embodiment 2;
Fig. 9 is a kind of working state figure of the liquid chromatograph of embodiment 3;
Figure 10 is a kind of working state figure of the liquid chromatograph of embodiment 3;
Wherein, 1 is interface a, and 2 is interface b, and 3 is interface c, and 4 is interface d, and 5 is interface e, and 6 is interface f, and 7 is interface g, and 8 is interface h, and 9 is interface i, and 10 is interface j, and 11 is port a, and 12 is port b, and 13 is port c, and 14 is port d, and 15 is port e, and 16 is port f; 17 are port g, 18 are port h, 19 are port i, 20 are ports, 21 are port k, 22 are port l, 23 are port m, 24 are port n, 25 are port o, 26 is port p; 27 are port q, 28 are port r, 29 are port s, 30 are port t, 31 are port u, 32 is port v;
AS is injector, and W1 is the first waste port, and W2 is the first waste port, and DE is detecting device; S1 is the first mobile phase, and S2 is the second mobile phase, and S3 is modulation solution, and S4 is calibration solution; P1 is discharge pump I, P2 be discharge pump II, P3 be discharge pump III, P4 be discharge pump IV, P5 is gasing pump; C1 is the first chromatographic column, and C2 is middle chromatographic column, and C3 is the second chromatographic column; L3 is first flow, and L13 is the second runner, and L14 analyzes runner, and L4 is waste liquid runner, and L11 is the first connecting line, and L18 is the second connecting line, and L5 is the 3rd connecting line, and L10 is the 3rd connecting line, and L17 is modulation runner; T1 is threeway I, T2 is threeway II; L7 is the first branch flow passage, and L20 is the second branch flow passage, and L24 is the 3rd branch flow passage, and Z1 is retaining valve I, Z2 is retaining valve II; L26 is gas input flow road, and L27 is gas exhaust manifold, and L25 is that the second waste liquid discharges runner, and L31 is calibration solution runner, and L34 is the 3rd waste liquid runner; V1 is multiple flow passages transfer valve, and V2 deposits valve, and V3 is solution replacing valve, and V4 corrects transfer valve; B1 is atmosphere storage ring, and B2 is calibration solution storage ring; In figure, real point represents sealed condition, and heavy line represents mobile phase glide path.
Embodiment
Below in conjunction with drawings and Examples, the utility model is made further explanation
Embodiment 1
As shown in Figure 1, a kind of liquid chromatograph, comprising: the first flow L3 being connected with injector AS and the first chromatographic column C1 in turn, for carrying the first mobile phase S1, and carries out initial gross separation to sample; Second runner L13, for carrying the second mobile phase S2; Be connected with the analysis runner L14 of the second chromatographic column C2, for carrying out separation and detection to the material of catching; First waste liquid runner L4, for getting rid of waste liquid;
Also comprise be provided with multiple interface multiple flow passages transfer valve V1, be provided with multiple port deposit valve V2, described in deposit valve V2 any two ends mouth between be connected with middle chromatographic column C2; And be provided with the solution replacing valve V3 of multiple port, be connected with atmosphere storage ring B1 between any two ends mouth of described solution replacing valve V3; Any three interfaces of described multiple flow passages transfer valve V1 are connected to one end of the first connecting line L11, the second connecting line L18 and the 3rd connecting line L5, described first connecting line L11 is communicated with two ports of solution replacing valve V3 respectively with the other end of the second connecting line L18; The other end of described 3rd connecting line L5 can be replaced with a four-way by threeway IT1, threeway IIT2(two threeways) be communicated with any two idle ports of a port and solution replacing valve V3 of depositing valve V2 respectively; Described first flow L3, the second runner L13, analysis runner L14 and waste liquid runner L4 are connected in all the other any one idle interface on multiple flow passages transfer valve V1; Describedly deposit the 4th connecting line L10 be provided with between valve V2 with solution replacing valve V3 for both being directly connected; All the other idle ports of described solution replacing valve V3 are also connected to gas input flow road L26, gas exhaust manifold L27 and the second waste liquid and discharge runner L25.
Concrete connected mode:
Described multiple flow passages transfer valve V1 comprises interface a1, interface b2, interface c3, interface d4, interface e5, interface f6, interface g7, interface h8, interface i9 and interface j10; Described interface a1 is connected with first flow L3, described interface b2 is connected with waste liquid runner L4, described interface c3 is connected with the first connecting line L11, described interface d4 is connected with analysis runner L14, described interface e5 is connected with the second runner L13, described interface f6 is connected with the second connecting line L18, and described interface j10 is connected with the 3rd connecting line L5.Described interface h8 and interface i9 is sealed condition.Described deposit valve V2 comprise described in deposit valve V2 and comprise port a11, port b12, port c13, port d14, port e15, port f16; Be provided with middle chromatographic column C2 between described port a11 and port d14, described port e15 is connected with the 3rd connecting line L5, and described port f16 is connected with the 4th connecting line L10.Described port b12 and port c13 is sealed condition.Described solution replacing valve V3 comprises port g17, port h18, port i19, port j20, port k21, port l22, port m23, port n24, port o25, port p26; Be provided with atmosphere storage ring B1 between described port m23 and port j20, port l22 be connected with gas input flow road L26, port k21 be connected with gas exhaust manifold L27; All the other ports are connected to the first connecting line L11, the second connecting line L18, the 4th connecting line L10, the second waste liquid discharge runner L25, and the two articles of branch lines be connected with the 3rd connecting line L5.Described two branch lines are preferably equipped with retaining valve.
Functional description:
As shown in Figure 2, first chromatographic column separation function: open discharge pump IP1, the first mobile phase S1 is made to vacillate dynamic under the promotion of IP1 downwards, sample imports in the first mobile phase by injector AS, the first mobile phase containing sample enters the first chromatographic column C1, and in sample, each component starts to be separated under the chromatographic resolution mechanism of the first mobile phase with the first chromatographic column, conducting multiple flow passages transfer valve V1 port a1 and port b2, make the first mobile phase flow to waste liquid runner L4, discharge.
As shown in Figure 3, middle chromatographic column capturing function: open discharge pump IP1, carry the first mobile phase S1 to downstream, successively through injector AS and the first chromatographic column C1, after sample imports to the first mobile phase by AS, in sample, target components is separated with the chromatographic effect power of the first chromatographic column by the first mobile phase, when target components in sample is about to flow out from the first chromatographic column time, conducting multiple flow passages transfer valve V1 port a1 and port j10, the first flowing containing target components was communicated the 3rd connecting line L5, threeway TI, first branch line L7 flows into and deposits valve V2, after flowing through middle chromatographic column C2 again, have with the second chromatographic column C2 and can be caught by the second chromatographic column C2 compared with the target components of high forces, the component of not catching with the first flowing in succession continuous vacillate downwards dynamic, after the 4th connecting pipeline L10 and the first connecting pipeline L11, flow to multiple flow passages transfer valve V1 port c3 and port b2, the first mobile phase is made to flow to waste liquid runner L4, discharge.
As shown in Figure 4, atmosphere storage function: complete in the random time section that this process can be connected with air pump at gas storage ring, namely, open gas transfer pump P5, gas is flowed into from gas input flow road L26, the port l22 of conducting solution replacing valve V3 and port m23, make gas by after gas storage ring B1, after entering the second waste liquid discharge runner L25 from gas exhaust manifold L27, discharge.
As shown in Figure 5, solution replacement function: under the prerequisite that the atmosphere storage function shown in Fig. 4 completes, open discharge pump IIP2, second mobile phase S2 is delivered to multiple flow passages transfer valve V1, through interface e5 and interface f6, solution replacing valve V3 is flowed into after second connecting line L18, after flowing through atmosphere storage ring B1, the gas prestored in atmosphere storage ring B1 is promoted by follow-up second mobile phase, continuation is vacillated dynamic downwards, from Three branched pipe road L24, third connecting pipe L5 is flowed into after threeway T2, flow into after threeway T1 and the first branch line L7 again and deposit valve V2, after entering middle chromatographic column C2, originally the liquid comprised in middle chromatographic column C2 is first released by gas, the second follow-up mobile phase enters middle chromatographic column C2, complete solution replacement function, the solution be replaced and gas continue to flow into the 4th connecting line L10 and get back to solution replacing valve V3, runner L25 is discharged again through the second waste liquid, discharge.
As shown in Figure 6, after completing the replacement of solution shown in Fig. 5, carry out the function analyzed: open discharge pump IIP2, second runner L13 is delivered to multiple flow passages transfer valve V1, through interface e5 and interface f6, solution replacing valve V3 is flowed into after second connecting line L18, again through the second branch line L20, flow into after threeway T1 and the first branch line and deposit valve V2, after flowing through middle chromatographic column C2, the target components that middle chromatographic column C2 catches is by the second mobile phase wash-out, the second flowing containing target components in succession continuous vacillate downwards dynamic, flow into the 4th connecting line L10 and get back to solution replacing valve V3, again after the first connecting line L11, flow into multiple flow passages transfer valve V1, flow into after analyzing runner L14 again, target components is separated at the second chromatographic column C3, detected by downstream detector DE.
Embodiment 2
As shown in Figure 7, a kind of liquid chromatograph, on the basis of embodiment 1, also comprise modulation runner L17, for carrying modulation solution, described modulation runner L17 is connected on any one interface of multiple flow passages transfer valve V1, and described interface is the adjacent interface with L18 connectivity port.Preferably, modulate runner L17 to be connected on the interface g7 of multiple flow passages transfer valve V1.By cleaning solution (being generally the solvent of water or low concentration), prewashing is carried out to the material be captured in middle chromatographic column, then carry out solution replacement, the impact of matrix can be eliminated further.
As shown in Figure 8, prewashing functional description: as shown in Figure 3 after chromatographic column capturing function, before the replacement function of solution shown in Fig. 5, a step can be added, prerinse is carried out to being captured in the material of catching in middle chromatographic column C2, open discharge pump IIIP3, assisted solution S3 is inputted multiple flow passages transfer valve V1 through auxiliary flow L17, flow into through the second connecting line again, solution replacing valve V3 is flowed into after second connecting line L18, again through the second branch line L20, flow into after threeway T1 and the first branch line and deposit valve V2, after flowing through middle chromatographic column C2, cleaned by assisted solution by the component mixed with assisted solution S3 in the component that middle chromatographic column C2 catches, flow into the 4th connecting line L10 and get back to solution replacing valve V3, again after the first connecting line L11, flow into multiple flow passages transfer valve V1, waste liquid runner L4 is flow to again from the first mobile phase, discharge.
Embodiment 3
As shown in Figure 9, Figure 10, a kind of liquid chromatograph, on the basis of embodiment 1, also comprises the correction transfer valve V4 being provided with multiple port, two ports of described correction transfer valve V4 are connected on described analysis runner L14, and are positioned at the front-end position of the second chromatographic column; Be connected with calibration solution storage ring B2 between any two idle ports of described correction transfer valve V4, all the other idle ports are also connected to calibration solution runner L31 and the 3rd waste liquid runner L34.
Concrete connected mode is: described correction transfer valve V4 comprises port q27, port r28, port s29, port t30, port u31, port v32; Be connected with calibration solution storage rings B2 between described port s29 and port v32, described port q27 and port r28 is connected to and analyzes on runner L14, and described port t30, port u31 are connected with calibration solution runner L31 and the 3rd waste liquid runner L34 respectively.
Calibration function describes:
As shown in Figure 9, calibration solution memory function: open discharge pump IV P4 and pass through calibration solution runner L31 entrance correction transfer valve V4 by containing the calibration solution S4 correcting component, again after calibration solution storage ring B2, make in calibration solution storage ring B2 containing calibration solution, unnecessary calibration solution, from the 3rd waste liquid runner L34, is discharged.
As shown in Figure 10, calibration function: open discharge pump IIP2, second runner L13 is delivered to multiple flow passages transfer valve V1, through interface e5 and interface f6, solution replacing valve V3 is flowed into after second connecting line L18, again through the second branch line L20, flow into after threeway T1 and the first branch line and deposit valve V2, after flowing through middle chromatographic column C2, flow into the 4th connecting line L10 and get back to solution replacing valve V3, again after the first connecting line L11, flow into multiple flow passages transfer valve V1, (also can conducting V1 4, 5 ports, that the second mobile phase S1 directly enters analysis runner L14, not necessarily need through depositing valve and replacing valve) flow into again through the and analyze entrance correction transfer valve V4 after runner L14, enter calibration solution storage ring B2 again, the calibration solution promoted in calibration solution storage ring B2 is vacillated dynamic downwards, correction material enters the second chromatographic column C3 and is separated, detected by downstream detector DE.
Claims (10)
1. a liquid chromatograph, comprising:
Being connected with the first flow (L3) of the first chromatographic column (C1), for carrying the first mobile phase (S1), and initial gross separation being carried out to sample;
Second runner (L13), for carrying the second mobile phase (S2);
Be connected with the analysis runner (L14) of the second chromatographic column (C2), for carrying out separation and detection to the material of catching;
First waste liquid runner (L4), for getting rid of waste liquid;
It is characterized in that, also comprise be provided with multiple interface multiple flow passages transfer valve (V1), be provided with multiple port deposit valve (V2), described in deposit valve (V2) any two ends mouth between be connected with middle chromatographic column (C2); And be provided with the solution replacing valve (V3) of multiple port, be connected with atmosphere storage ring (B1) between any two ends mouth of described solution replacing valve (V3);
Any three interfaces of described multiple flow passages transfer valve (V1) are connected with one end of the first connecting line (L11), the second connecting line (L18) and the 3rd connecting line (L5) respectively, and described first connecting line (L11) is communicated with two ports of solution replacing valve (V3) respectively with the other end of the second connecting line (L18); The other end of described 3rd connecting line (L5) is not communicated with any two idle ports of a port and solution replacing valve (V3) of depositing valve (V2) by many reduction of fractions to a common denominators; Described first flow (L3), the second runner (L13), analysis runner (L14) and waste liquid runner (L4) are connected in all the other any one idle interface on multiple flow passages transfer valve (V1);
Describedly deposit the 4th connecting line (L10) be provided with between valve (V2) with solution replacing valve (V3) for both being directly connected;
All the other idle ports of described solution replacing valve (V3) are also connected to gas input flow road (L26), gas exhaust manifold (L27) and the second waste liquid and discharge runner (L25).
2. liquid chromatograph according to claim 1, it is characterized in that, also comprise modulation runner (L17), for carrying modulation solution, described modulation runner (L17) is connected in an idle interface of multiple flow passages transfer valve (V1), and this idle interface is adjacent with the interface being connected with the second connecting line (L18).
3. liquid chromatograph according to claim 1 or 2, it is characterized in that, also comprise the correction transfer valve (V4) being provided with multiple port, two ports of described correction transfer valve (V4) are connected on described analysis runner (L14), and are positioned at the front-end position of the second chromatographic column (C2); Be connected with calibration solution storage ring (B2) between any two idle ports of described correction transfer valve (V4), all the other idle ports are also connected to calibration solution runner (L31) and the 3rd waste liquid runner (L34).
4. liquid chromatograph according to claim 1 or 2, it is characterized in that, described multiple flow passages transfer valve (V1) comprises interface a (1), interface b (2), interface c (3), interface d (4), interface e (5), interface f (6), interface g (7) and interface j (10); Described interface a (1) is connected with first flow (L3), described interface b (2) is connected with waste liquid runner (L4), described interface c (3) is connected with the first connecting line (L11), described interface d (4) is connected with analysis runner (L14), described interface e (5) is connected with the second runner (L13), described interface f (6) is connected with the second connecting line (L18), and described interface j (10) is connected with the 3rd connecting line (L5).
5. liquid chromatograph according to claim 4, is characterized in that, described interface g (7) is connected with modulation runner (L17).
6. liquid chromatograph according to claim 1 or 2, it is characterized in that, described in deposit valve (V2) and comprise port a (11), port b (12), port c (13), port d (14), port e (15), port f (16); Middle chromatographic column (C2) is provided with between described port a (11) and port d (14), described port e (15) is connected with the 3rd connecting line (L5), and described port f (16) is connected with the 4th connecting line (L10).
7. liquid chromatograph according to claim 6, it is characterized in that, described port b (12) and port c (13) are sealed condition.
8. liquid chromatograph according to claim 1 or 2, it is characterized in that, described solution replacing valve (V3) comprises port g (17), port h (18), port i (19), port j (20), port k (21), port l (22), port m (23), port n (24), port o (25), port p (26); Atmosphere storage ring (B1) is provided with between described port m (23) and port j (20), port l (22) is connected with gas input flow road (L26), port k (21) is connected with gas exhaust manifold (L27); All the other ports are connected to the first connecting line (L11), the second connecting line (L18), the 4th connecting line (L10), the second waste liquid discharge runner (L25), and the two articles of branch lines be connected with the 3rd connecting line (L5).
9. liquid chromatograph according to claim 8, is characterized in that, described two branch lines are equipped with retaining valve.
10. liquid chromatograph according to claim 3, it is characterized in that, described correction transfer valve (V4) comprises port q (27), port r (28), port s (29), port t (30), port u (31), port v (32); Calibration solution storage rings (B2) are connected with between described port s (29) and port v (32), described port q (27) and port r (28) are connected to and analyze on runner (L14), and described port t (30), port u (31) are connected with calibration solution runner (L31) and the 3rd waste liquid runner (L34) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520096836.6U CN204405612U (en) | 2015-02-11 | 2015-02-11 | A kind of liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201520096836.6U CN204405612U (en) | 2015-02-11 | 2015-02-11 | A kind of liquid chromatograph |
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| CN204405612U true CN204405612U (en) | 2015-06-17 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104678015A (en) * | 2015-02-11 | 2015-06-03 | 王峰 | Liquid chromatograph |
| CN105688443A (en) * | 2016-02-05 | 2016-06-22 | 北京创新通恒科技有限公司 | Chromatography purification and separation system and separation and purification method |
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2015
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104678015A (en) * | 2015-02-11 | 2015-06-03 | 王峰 | Liquid chromatograph |
| CN104678015B (en) * | 2015-02-11 | 2016-11-16 | 王峰 | A kind of chromatograph of liquid |
| CN105688443A (en) * | 2016-02-05 | 2016-06-22 | 北京创新通恒科技有限公司 | Chromatography purification and separation system and separation and purification method |
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