CN204347041U - A kind of full-automatic lipoprotein class detector - Google Patents
A kind of full-automatic lipoprotein class detector Download PDFInfo
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- CN204347041U CN204347041U CN201420800543.7U CN201420800543U CN204347041U CN 204347041 U CN204347041 U CN 204347041U CN 201420800543 U CN201420800543 U CN 201420800543U CN 204347041 U CN204347041 U CN 204347041U
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- liquid groove
- buffering liquid
- gel column
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- automatic
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- 108090001030 Lipoproteins Proteins 0.000 title claims abstract description 42
- 102000004895 Lipoproteins Human genes 0.000 title claims abstract description 42
- 230000003139 buffering effect Effects 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 238000010191 image analysis Methods 0.000 claims abstract description 14
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 238000013016 damping Methods 0.000 claims description 28
- 239000012530 fluid Substances 0.000 claims description 28
- 230000005284 excitation Effects 0.000 claims description 22
- 125000006850 spacer group Chemical group 0.000 claims description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 108010010234 HDL Lipoproteins Proteins 0.000 description 3
- 102000015779 HDL Lipoproteins Human genes 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000032928 Dyslipidaemia Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 108010023302 HDL Cholesterol Proteins 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 208000017170 Lipid metabolism disease Diseases 0.000 description 1
- 208000001145 Metabolic Syndrome Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Abstract
The utility model discloses a kind of full-automatic lipoprotein class detector, comprise classification and Detection case, image analysis processing system and printer, classification and Detection case comprises casing and upper cover, the upper part of casing is provided with buffering liquid groove, the lower part of casing is provided with lower buffering liquid groove, the middle part of casing is magazine, side shield is provided with in magazine, magazine is divided into gel column compartment and light source chamber by side shield, gel column compartment is provided with gel column, the corresponding gel post holes of elastic sealing gasket hermetically passing is passed through in the upper end of each gel column, electrode is equipped with in upper buffering liquid groove and lower buffering liquid groove, two electrodes are connected to positive pole and the negative pole of power supply respectively by circuit, light supply apparatus and gel lamp is provided with in light source chamber, Photo Studio is provided with outside casing on front side of gel column compartment, image analysis processing system is connected with CCD camera, printer is connected with image analysis processing system, the utility model automaticity is high, lipoprotein class is effective, lipoprotein class efficiency is high.
Description
Technical field
The utility model relates to lipoprotein class field, specifically, is a kind of full-automatic lipoprotein class detector.
Background technology
Angiocardiopathy has become and has caused dead No.1 disease in the world, and traditional examination criteria and method can not disclose all paathogenic factors, and the lipid levels display of the cardiovascular patient of about 50% is normal.Only have the cardiac of 30% can be found according to detection total cholesterol level, even if according to existing international ATP III examination criteria, most angiocardiopathy can not be detected, and many low-density and the normal patient of high-density lipoprotein (HDL) still have the risk suffering from angiocardiopathy.These illustrate and detect overall low density cholesterol (LDL) and HDL-C (HDL) content is not the best index of angiocardiopathy preventing, it is increase cardiovascular disease incidence and dead independent hazard factor that blood T-CHOL and low-density lipoprotein cholesterol level raise, it is also one of component in metabolic syndrome while of dyslipidemia, the hot issue in lipid metaboli basis and clinical research field is lipid metaboli and heredity in recent years, the interaction of environmental factor and newtype drug, emphasis is inquired into complicacy disorders of lipid metabolism and is caused angiocardiopathy pathology, fatty liver, diabetes, and the problem lipoprotein class detection system such as kidney damage will make medical research work more efficient more convenient.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, adapts to reality need, provides a kind of full-automatic lipoprotein class detector.
For achieving the above object, the technical solution adopted in the present invention is:
A kind of full-automatic lipoprotein class detector, comprise classification and Detection case, described classification and Detection case comprises casing and upper cover, described upper cover covers the top at casing, box house is by being provided with upper spacer and described casing is divided into upper part by lower clapboard, middle part and lower part, the upper part of casing is provided with buffering liquid groove, the both sides of upper buffering liquid groove are respectively equipped with upper shed and under shed, the upper shed of upper buffering liquid groove and under shed are connected in upper damping fluid bottle and upper damping fluid receiving flask respectively by pipeline, the lower part of casing is provided with lower buffering liquid groove, the both sides of lower buffering liquid groove are respectively equipped with upper shed and under shed, the upper shed of lower buffering liquid groove and under shed are connected in lower damping fluid bottle and lower damping fluid receiving flask respectively by pipeline, the middle part of casing is magazine, side shield is provided with in magazine, described magazine is divided into gel column compartment and light source chamber by side shield, gel column compartment is provided with one or more gel column, gel column compartment upper and lower is equipped with multiple through hole, each through hole is equipped with a gel column holder, gel column is through the through hole of described gel column compartment upper and lower, the bottom of upper buffering liquid groove has multiple gel post holes, each gel post holes is provided with elastic sealing gasket, the corresponding gel post holes of elastic sealing gasket hermetically passing is passed through in the upper end of each gel column, electrode is equipped with in upper buffering liquid groove and described lower buffering liquid groove, two electrodes are connected to positive pole and the negative pole of power supply respectively by circuit, light supply apparatus and gel lamp is provided with in described light source chamber, position corresponding with each gel column above described side shield is provided with gel lamp, Photo Studio is provided with outside casing on front side of gel column compartment.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, also comprise image analysis processing system and printer, image analysis processing system is connected with CCD camera, and printer is connected with described image analysis processing system.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, in the pipeline that upper shed and the under shed of upper buffering liquid groove are connected with upper damping fluid bottle and upper damping fluid receiving flask respectively and in the pipeline that is connected with lower damping fluid bottle and lower damping fluid receiving flask respectively of the upper shed of lower buffering liquid groove and under shed, be equipped with solenoid valve.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, light supply apparatus comprises multiple excitation source, and multiple excitation source is circular distribution centered by disk, and multiple excitation source is the light emitting diode of different wave length.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, many gel columns are parallel to be distributed in described gel column compartment.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, the both sides of upper buffering liquid groove, the both sides of lower buffering liquid groove connect as one with the both sides of casing respectively, and the bottom of upper buffering liquid groove and the bottom of casing connect as one.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, multiple gel post holes bottom multiple through hole of gel column compartment upper and lower and upper buffering liquid groove are all corresponding in vertical direction along parallel direction distribution.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, light supply apparatus comprises multiple excitation source, disk and rotary electric machine, multiple excitation source is mounted on disk, disk is mounted to rotary electric machine, rotary electric machine is fixed on the backboard of light source chamber described casing below, and the position that magazine side shield is corresponding with one of them excitation source of multiple excitation source is provided with focalizer.
In the disclosed a kind of full-automatic lipoprotein class detector above-described embodiment of the utility model, be provided with CCD camera in Photo Studio, CCD camera is connected to the top of described Photo Studio, and the position that cabinet front is corresponding with camera front has camera hole.
Beneficial effect
The disclosed a kind of full-automatic lipoprotein class detector beneficial effect of the utility model is:
1. automaticity is high;
2. lipoprotein class is effective;
3. lipoprotein class efficiency is high.
Accompanying drawing explanation
A kind of outboard profile cut-open view of full-automatic lipoprotein class detector disclosed in Fig. 1 the utility model;
A kind of front view (FV) cut-open view of full-automatic lipoprotein class detector disclosed in Fig. 2 the utility model;
Embodiment
For summary of the invention of the present utility model, Characteristic can be understood further, hereby exemplify following examples, and coordinate accompanying drawing to be described in detail as follows:
As depicted in figs. 1 and 2, the disclosed a kind of full-automatic lipoprotein class detector of the utility model, comprise classification and Detection case 01, classification and Detection case 01 comprises casing 04 and upper cover 05, upper cover 05 covers the top at casing 04, casing 04 inside is by being provided with upper spacer 06 and casing is divided into upper part 08 by lower clapboard 07, middle part 09 and lower part 10, the upper part 08 of casing 04 is provided with buffering liquid groove 11, the both sides of upper buffering liquid groove 11 are respectively equipped with upper shed and under shed, the upper shed of upper buffering liquid groove 11 and under shed are connected in upper damping fluid bottle 12 and upper damping fluid receiving flask 13 respectively by pipeline, the lower part of casing 04 is provided with lower buffering liquid groove 14, the both sides of lower buffering liquid groove 14 are respectively equipped with upper shed and under shed, the upper shed of lower buffering liquid groove 14 and under shed are connected in lower damping fluid bottle 15 and lower damping fluid receiving flask 16 respectively by pipeline, the middle part 09 of casing 04 is magazine, side shield 17 is provided with in magazine, magazine is divided into gel column compartment 18 and light source chamber 19 by side shield 17, gel column compartment 18 is provided with one or more gel column 20, gel column compartment 18 upper and lower is equipped with multiple through hole, each through hole is equipped with a gel column holder 21, gel column holder 21 is for accommodating tightly by gel column 20, control gel column 20 comes off, gel column 20 is through the through hole of gel column compartment 18 upper and lower, the bottom of upper buffering liquid groove 11 has multiple gel post holes, each gel post holes is provided with elastic sealing gasket, the corresponding gel post holes of elastic sealing gasket hermetically passing is passed through in the upper end of each gel column 20, many gel columns 20 are parallel to be distributed in gel column compartment 18, multiple gel post holes bottom multiple through hole of gel column compartment 18 upper and lower and upper buffering liquid groove 11 are all corresponding in vertical direction along parallel direction distribution, electrode 29 is equipped with in upper buffering liquid groove 11 and lower buffering liquid groove 14, two electrodes 29 are connected to positive pole and the negative pole of power supply respectively by circuit, light supply apparatus and gel lamp 33 is provided with in light source chamber 19, position corresponding with each gel column 20 above described side shield 17 is provided with gel lamp 33, light supply apparatus comprises multiple excitation source 22, the circular distribution centered by disk 23 of multiple excitation source 22, multiple excitation source 22 is the light emitting diode of different wave length, light supply apparatus comprises multiple excitation source 22, disk 23 and rotary electric machine 24, multiple excitation source 22 is mounted on described disk 23, disk 23 is mounted to described rotary electric machine 24, rotary electric machine 24 is fixed on the backboard of described light source chamber 19 described casing 04 below, the position that magazine side shield 17 is corresponding with one of them excitation source of described multiple excitation source 22 is provided with focalizer 25, Photo Studio 26 is provided with outside casing on front side of gel column compartment 18, CCD camera 27 is provided with in Photo Studio, CCD camera 27 is connected to the top of described Photo Studio 26, position corresponding with camera 27 front on front side of casing 04 has camera hole 28.
As shown in Figure 1, a kind of full-automatic lipoprotein class detector, also comprise image analysis processing system 02 and printer 03, image analysis processing system 02 is connected with CCD camera 27, and printer 03 is connected with image analysis processing system 02.
As shown in Figure 2, a kind of full-automatic lipoprotein class detector, is equipped with solenoid valve 32 in the pipeline that upper shed and the under shed of upper buffering liquid groove 11 are connected with upper damping fluid bottle 12 and upper damping fluid receiving flask 13 respectively and in the pipeline that is connected with lower damping fluid bottle 15 and lower damping fluid receiving flask 16 respectively of the upper shed of lower buffering liquid groove 14 and under shed.The both sides of upper buffering liquid groove 11, the both sides of lower buffering liquid groove 14 connect as one with the both sides of casing 04 respectively, the bottom of upper buffering liquid groove 11 and the bottom of casing 04 connect as one, the damping fluid that all must more renew tested by damping fluid in upper buffering liquid groove 11 at every turn, reusable at least 5 times of damping fluid in lower buffering liquid groove 14, need not test all exchange buffering liquid at every turn.
The sample to be separated of a kind of full-automatic lipoprotein class detector of the utility model can be take from the blood serum sample of humans and animals or add the blood serum sample of EDTA.
Cleaning Principle of the present utility model: in serum, various protein has its distinctive isoelectric point, higher than in the damping fluid of its isoelectric point PH, by forming electronegative particle, to positive pole swimming under electric field action.Under identical conditions, different proteins is electrically charged variant, and molecular size range is also different, so mobility speed is different, lipoprotein can be made a distinction.Lipoprotein is due to molecular size difference, and in polyacrylamide gel, mobility is also different, thus reaches the object of separation, namely obtains the classification of lipoprotein.
Disclosed in the utility model, a kind of operation steps of full-automatic lipoprotein class detector is as follows:
Step one: the gel column 20 that testing sample is housed is arranged in gel chamber 18 by through hole;
Step 2: the light supply apparatus 19 opening light source chamber carries out gel reaction 30min, can regulate excitation source 22 according to the difference of sample to be separated by the disk 23 on rotary electric machine 24;
Step 3: after focusing reaction completes, close the light supply apparatus 19 of light source chamber, open high-voltage power supply, voltage is set to 500V, electrophoresis starts;
Step 4: after electrophoresis terminates, open Photo Studio 27, make a video recording, the classification results of gel column lipoprotein is transferred to image analysis processing system 02, open analysis software in image analysis processing system 02, analyze glue figure available printers 03 and the classification results of lipoprotein is printed.
Last it is noted that obviously, above-described embodiment is only for the utility model example is clearly described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of amplifying out or variation be still among the protection domain of this explanation.
Claims (9)
1. a full-automatic lipoprotein class detector, comprise classification and Detection case, described classification and Detection case comprises casing and upper cover, described upper cover covers the top at described casing, described box house is by being provided with upper spacer and described casing is divided into upper part by lower clapboard, middle part and lower part, the upper part of described casing is provided with buffering liquid groove, the both sides of described upper buffering liquid groove are respectively equipped with upper shed and under shed, the upper shed of described upper buffering liquid groove and under shed are connected in upper damping fluid bottle and upper damping fluid receiving flask respectively by pipeline, the lower part of described casing is provided with lower buffering liquid groove, the both sides of described lower buffering liquid groove are respectively equipped with upper shed and under shed, the upper shed of described lower buffering liquid groove and under shed are connected in lower damping fluid bottle and lower damping fluid receiving flask respectively by pipeline, the middle part of described casing is magazine, side shield is provided with in described magazine, described magazine is divided into gel column compartment and light source chamber by described side shield, described gel column compartment is provided with one or more gel column, described gel column compartment upper and lower is equipped with multiple through hole, described each through hole is equipped with a gel column holder, described gel column is through the through hole of described gel column compartment upper and lower, the bottom of described upper buffering liquid groove has multiple gel post holes, described each gel post holes is provided with elastic sealing gasket, the corresponding gel post holes of elastic sealing gasket hermetically passing is passed through in the upper end of described each gel column, electrode is equipped with in described upper buffering liquid groove and described lower buffering liquid groove, described two electrodes are connected to positive pole and the negative pole of power supply respectively by circuit, light supply apparatus and gel lamp is provided with in described light source chamber, position corresponding with each gel column above described side shield is provided with gel lamp, Photo Studio is provided with outside casing on front side of described gel column compartment.
2. a kind of full-automatic lipoprotein class detector as claimed in claim 1, characterized by further comprising image analysis processing system and printer, described image analysis processing system is connected with CCD camera, and described printer is connected with described image analysis processing system.
3. lipoprotein class detector as claimed in claim 1 a kind of full-automatic, is characterized in that: be equipped with solenoid valve in the pipeline that upper shed and the under shed of described upper buffering liquid groove are connected with upper damping fluid bottle and upper damping fluid receiving flask respectively and in the pipeline that is connected with lower damping fluid bottle and lower damping fluid receiving flask respectively of the upper shed of described lower buffering liquid groove and under shed.
4. a kind of full-automatic lipoprotein class detector as claimed in claim 1, it is characterized in that: described light supply apparatus comprises multiple excitation source, the circular distribution centered by disk of described multiple excitation source, described multiple excitation source is the light emitting diode of different wave length.
5. a kind of full-automatic lipoprotein class detector as claimed in claim 1, is characterized in that: described many gel columns are parallel to be distributed in described gel column compartment.
6. a kind of full-automatic lipoprotein class detector as claimed in claim 1, it is characterized in that: the both sides of described upper buffering liquid groove, the both sides of described lower buffering liquid groove connect as one with the both sides of described casing respectively, and the bottom of described upper buffering liquid groove and the bottom of described casing connect as one.
7. lipoprotein class detector as claimed in claim 1 a kind of full-automatic, is characterized in that: multiple gel post holes bottom multiple through hole of described gel column compartment upper and lower and described upper buffering liquid groove are all corresponding in vertical direction along parallel direction distribution.
8. a kind of full-automatic lipoprotein class detector as claimed in claim 1, it is characterized in that: described light supply apparatus comprises multiple excitation source, disk and rotary electric machine, described multiple excitation source is mounted on described disk, described disk is mounted to described rotary electric machine, described rotary electric machine is fixed on the backboard of described light source chamber described casing below, and the position that described magazine side shield is corresponding with one of them excitation source of described multiple excitation source is provided with focalizer.
9. a kind of full-automatic lipoprotein class detector as claimed in claim 1, it is characterized in that: in described Photo Studio, be provided with CCD camera, described CCD camera is connected to the top of described Photo Studio, and the position that described cabinet front is corresponding with described camera front has camera hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420800543.7U CN204347041U (en) | 2014-12-18 | 2014-12-18 | A kind of full-automatic lipoprotein class detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420800543.7U CN204347041U (en) | 2014-12-18 | 2014-12-18 | A kind of full-automatic lipoprotein class detector |
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| CN204347041U true CN204347041U (en) | 2015-05-20 |
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| CN201420800543.7U Active CN204347041U (en) | 2014-12-18 | 2014-12-18 | A kind of full-automatic lipoprotein class detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109100305A (en) * | 2018-07-13 | 2018-12-28 | 陕西师范大学 | A kind of the digitlization material information acquisition device and method of liquid disperse system |
-
2014
- 2014-12-18 CN CN201420800543.7U patent/CN204347041U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109100305A (en) * | 2018-07-13 | 2018-12-28 | 陕西师范大学 | A kind of the digitlization material information acquisition device and method of liquid disperse system |
| CN109100305B (en) * | 2018-07-13 | 2022-02-18 | 陕西师范大学 | Digital substance information acquisition device and method for liquid dispersion system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
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| TR01 | Transfer of patent right |
Effective date of registration: 20190617 Address after: 101400 Yang Yan Road 88, Yan Qi Economic Development Zone, Huairou District, Beijing Patentee after: Ariel (Beijing) Biotechnology Co.,Ltd. Address before: 100097 Unit 1E, Building 7, Guanshan Garden, Lanindichang Road, Haidian District, Beijing Patentee before: Fu Hu |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231201 Address after: Room 303-71, 3rd Floor, Building 4, No. 9 Yike Road, Life Science Park, Changping District, Beijing, 102200 (Cluster Registration) Patentee after: Beijing Guoxuan Youpin International Cold Chain Logistics Technology Co.,Ltd. Address before: 101400 Yang Yan Road 88, Yan Qi Economic Development Zone, Huairou District, Beijing Patentee before: Ariel (Beijing) Biotechnology Co.,Ltd. |