CN204265760U - The location of biological polar analytes and concentrating unit - Google Patents
The location of biological polar analytes and concentrating unit Download PDFInfo
- Publication number
- CN204265760U CN204265760U CN201420741443.1U CN201420741443U CN204265760U CN 204265760 U CN204265760 U CN 204265760U CN 201420741443 U CN201420741443 U CN 201420741443U CN 204265760 U CN204265760 U CN 204265760U
- Authority
- CN
- China
- Prior art keywords
- electric field
- storage pool
- biological
- passage
- glass plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of location and concentrating unit of biological polar analytes, belong to the polarity biomagnification technology in biological technical field.Comprise analyte storage pool, waste liquid storage pool and be communicated with the passage of analyte storage pool and waste liquid storage pool, described analyte storage pool and waste liquid storage pool are connected to a direct supply as a pair DC electrode, the both sides of passage are connected to an AC power as a pair ac electrode, direct supply and AC power form superposition of electric field on passage, and the intensity of alternating-electric field equals or is slightly better than DC electric field, superposition of electric field place is provided with micropore glass plate, and micropore glass plate has biological semi-permeable film.Superposition of electric field can make polar material stagnate and no longer move ahead along passage, forms polar analytes enrichment region, and micropore glass plate and biological semi-permeable film filter with concentrated further to polar material, thus complete location and the concentration process of polar material.
Description
Technical field
The utility model relates to a kind of location and concentrating unit of biological polar analytes, belongs to the polar material beneficiation technologies in biological technical field.
Background technology
The characteristic of biological substance and functional study need this large amount of materials, and a lot of biological substance naturally to there is enrichment degree low, artificial preparation concentration is little, needs to concentrate, and carries out the research of being correlated with after improving the concentration of material again.Existing physico-chemical process mainly contains absorption, precipitation, centrifugal and evaporation, and these methods waste time and energy and can affect the activity of biological substance; Molecular biology method mainly contains Suppression subtractive hybridization etc., and this method complicated operation, cost are high and be only applicable to gene.
Summary of the invention
For the defect of prior art, the utility model provides a kind of location and concentrating unit of biological polar analytes, its have easy and simple to handle, enrichment factor is high and do not affect the advantage of biological polar material activity.
In order to solve the technical problem, the technical scheme that the utility model provides is: a kind of location of biological polar analytes and concentrating unit, comprise the analyte storage pool of storage polar analytes, the waste liquid storage pool of storage waste liquid and the passage of connection analyte storage pool and waste liquid storage pool, described analyte storage pool and waste liquid storage pool are connected to a direct supply as a pair DC electrode, the both sides of passage are connected to an AC power as a pair ac electrode, direct supply and AC power form superposition of electric field on passage, and the intensity of alternating-electric field equals or is slightly better than DC electric field, superposition of electric field place is provided with micropore glass plate, micropore glass plate has biological semi-permeable film.The intensity of superposition of electric field place alternating-electric field and frequency can make polar material stagnate and no longer move ahead along passage, thus form polar analytes enrichment region,
Further, described direct supply forms DC electric field on the length direction of passage, and described AC power forms alternating-electric field on the width of passage, and DC electric field and alternating-electric field form superposition of electric field on passage.
Further, be provided with enrichment of analytes storage pool below described micropore glass plate, biological semi-permeable film is positioned in enrichment of analytes storage pool.
Further, on described micropore glass plate, the diameter of micropore is 0.5-50 μm.
The beneficial effects of the utility model: the utility model utilizes most of biological substance (as nucleic acid, protein, virus, cell, organoid etc.) can produce the principle of moment of dipole with electric field movement in the electric field, a direct supply is applied at the two ends of analyte storage pool and waste liquid storage pool, an AC power is applied in the both sides of passage, biological substance stops moving along passage at the move under influence of DC electric field to superposition of electric field, completes location and the enrichment of biological substance.Superposition of electric field place is provided with micropore glass plate and biological semi-permeable film in addition, can complete the filtration of biological substance with concentrated.The location of biological polar analytes described in the utility model and concentrating unit do not affect the activity of biological substance, and easy and simple to handle, and enrichment factor is high.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is side-looking structural representation of the present utility model;
In figure: 1, analyte storage pool, 2, waste liquid storage pool, 3, passage, 4, direct supply, 5, AC power, 6, micropore glass plate, 7, biological semi-permeable film, 8, enrichment of analytes storage pool.
Embodiment
Below in conjunction with drawings and the specific embodiments the utility model be described further and limit.
As Fig. 1, shown in 2, a kind of location of biological polar analytes and concentrating unit, comprise the analyte storage pool 1 of storage polar analytes, the waste liquid storage pool 2 of storage waste liquid and the passage 3 of connection analyte storage pool 1 and waste liquid storage pool 2, described analyte storage pool 1 and waste liquid storage pool 2 are connected to a direct supply 4 as a pair DC electrode, the both sides of passage 3 are connected to an AC power 5 as a pair ac electrode, direct supply 4 and AC power 5 form superposition of electric field on passage, the intensity of alternating-electric field equals or is slightly better than DC electric field, therefore polar material can be made to stagnate and no longer move ahead along passage, thus form polar analytes enrichment region, superposition of electric field place is provided with micropore glass plate 6, micropore glass plate 6 has biological semi-permeable film 7.
In the present embodiment, described direct supply 4 forms DC electric field on the length direction of passage 3, and described AC power 5 forms alternating-electric field on the width of passage 3, and DC electric field and alternating-electric field form superposition of electric field on passage 3.
In the present embodiment, described micropore glass plate 6 is provided with enrichment of analytes storage pool 8 below, and biological semi-permeable film 7 is positioned at enrichment of analytes storage pool 8 li.In the present embodiment, the diameter of described micropore glass 6 is 20 μm.
Working process of the present utility model: (1) fills it up with the conventional damping fluid (as TAE) containing electrolytic ion on waste liquid storage pool 2 and connecting passage 3; (2) in analyte storage pool 1, add biological polar analytes (mainly comprising nucleic acid, protein, virus etc.), connect direct supply 4 and AC power 5; (3) biological polar analytes slowly moves under the effect of DC electric field, until arrive superposition of electric field district; (4) polar analytes stops moving forward under the effect of two electric fields, stagnates in superposition of electric field, forms the enrichment region of polar material; (5) micropore glass plate 6 is positioned at superposition of electric field place, and it is lower is biological semi-permeable membranes 7, and polar analytes progressively through micropore glass plate 6, can enter the space that micropore glass plate 6 is formed with biological semi-permeable film 7 under gravity; (6) biological semi-permeable film 7 allows the small molecules such as water, ion to pass through, and the water in polar analytes liquid now and ion are slowly by biological semi-permeable film 7, and biological substance is stayed on biological semi-permeable film 7, completes the concentration process of polarity biological substance further.
Claims (4)
1. the location of a biological polar analytes and concentrating unit, it is characterized in that: the analyte storage pool (1) comprising storage polar analytes, the waste liquid storage pool (2) of storage waste liquid and the passage (3) of connection analyte storage pool (1) and waste liquid storage pool (2), described analyte storage pool (1) and waste liquid storage pool (2) are connected to a direct supply (4) as a pair DC electrode, the both sides of passage (3) are connected to an AC power (5) as a pair ac electrode, direct supply (4) and AC power (5) form superposition of electric field on passage (3), and the intensity of alternating-electric field equals or is slightly better than DC electric field, superposition of electric field place is provided with micropore glass plate (6), micropore glass plate (6) has biological semi-permeable film (7).
2. the location of biological polar analytes according to claim 1 and concentrating unit, it is characterized in that: described direct supply (4) forms DC electric field on the length direction of passage (3), described AC power (5) forms alternating-electric field on the width of passage (3), and DC electric field and alternating-electric field form superposition of electric field on passage.
3. the location of biological polar analytes according to claim 1 and 2 and concentrating unit, it is characterized in that: described micropore glass plate (6) is provided with enrichment of analytes storage pool (8) below, and it is inner that biological semi-permeable film (7) is positioned at enrichment of analytes storage pool (8).
4. the location of biological polar analytes according to claim 1 and 2 and concentrating unit, is characterized in that: the diameter of the upper micropore of described micropore glass plate (6) is 0.5-50 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420741443.1U CN204265760U (en) | 2014-12-02 | 2014-12-02 | The location of biological polar analytes and concentrating unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420741443.1U CN204265760U (en) | 2014-12-02 | 2014-12-02 | The location of biological polar analytes and concentrating unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204265760U true CN204265760U (en) | 2015-04-15 |
Family
ID=52800638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420741443.1U Expired - Fee Related CN204265760U (en) | 2014-12-02 | 2014-12-02 | The location of biological polar analytes and concentrating unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204265760U (en) |
-
2014
- 2014-12-02 CN CN201420741443.1U patent/CN204265760U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Feng et al. | Bioinspired energy conversion in nanofluidics: a paradigm of material evolution | |
| Velegol et al. | Origins of concentration gradients for diffusiophoresis | |
| Marrone et al. | Review of the harvesting and extraction program within the National Alliance for Advanced Biofuels and Bioproducts | |
| CN106044970A (en) | Method for flow-electrode capacitive deionization (FCDI)-based desalination and application | |
| Santra et al. | Recent trends on micro/nanofluidic single cell electroporation | |
| CN110723769B (en) | Continuous seawater desalination device and method | |
| CN102174388B (en) | Surface-electrode-technology-based high-throughput cell electrofusion chip device | |
| JP2008532733A5 (en) | ||
| CN209302785U (en) | Micro-fluidic chip, the device containing the micro-fluidic chip | |
| JP2013021959A5 (en) | ||
| Alhattab et al. | Dispersed air flotation and foam fractionation for the recovery of microalgae in the production of biodiesel | |
| KR20160066891A (en) | Paper based preconcentrating method, preconcentrator and manufacturing method thereof | |
| CN104229955A (en) | ED (electrodialysis) water treatment system with intelligent temperature-adjusting, voltage-adjusting and ultrasonic effects | |
| CN110004307B (en) | Salt lake lithium extraction device based on ion concentration polarization effect | |
| CN104241517A (en) | Method for converting mechanical energy into electric energy by means of graphene hydrogel thin films of layered structure | |
| Chen et al. | Characterization of particle movement and high-resolution separation of microalgal cells via induced-charge electroosmotic advective spiral flow | |
| CN104974997A (en) | New cell separation method using parallel electric field type photoelectric chip | |
| CN204265760U (en) | The location of biological polar analytes and concentrating unit | |
| CN104807873B (en) | A kind of multi-functional agarose gel electrophoresis groove | |
| Elewa et al. | A comparison of capacitive deionization and membrane capacitive deionization using novel fabricated ion exchange membranes | |
| CN109354294A (en) | It is a kind of to mitigate polarized distillation device using capacitive deionization | |
| Kalyanasundaram et al. | Electric field-induced concentration and capture of DNA onto microtips | |
| CN105347447B (en) | Utilize the method for Electrostatic Absorption purification high-salt wastewater | |
| CN109270153A (en) | A kind of no ampholytes free flow isoelectric focusing electrophoresis separation method | |
| CN103789199B (en) | A kind of continuous reaping apparatus of micro-algae and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150415 Termination date: 20201202 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |