CN204265741U

CN204265741U - A kind of conventional phenol-chloroform extraction process Isolation and purification DNA test kit

Info

Publication number: CN204265741U
Application number: CN201420728603.9U
Authority: CN
Inventors: 李国治; 张建群; 苟潇; 邓凯元; 杨志雷; 唐伟杰
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2014-11-28
Filing date: 2014-11-28
Publication date: 2015-04-15
Anticipated expiration: 2024-11-28

Abstract

The utility model relates to a kind of conventional phenol-chloroform extraction process Isolation and purification DNA test kit, described one conventional phenol-chloroform extraction process Isolation and purification DNA test kit primary structure comprises: box body, lid, lid switch, Reagent Tube, pipe lid, bracing frame, base, the all reagent set used by conventional phenol-chloroform extraction process Isolation and purification DNA are in a test kit, concentrate and carry out experimental implementation, described test kit entirety all manufactures with synthetic glass, Reagent Tube pipe shaft indicates scale, handled easily person observation experiment data, drip experimental solutions, provide conveniently for operator carries out test operation, described Reagent Tube is fixed on test kit inside by bracing frame and base, effectively prevent Reagent Tube to tilt to cause the situation of escape to occur, improve extraction efficiency, structure is simple, easy and simple to handle.

Description

A kind of conventional phenol-chloroform extraction process Isolation and purification DNA test kit

Technical field

The utility model relates to biological technical field, specifically, relates to a kind of conventional phenol-chloroform extraction process

Isolation and purification DNA test kit.

Background technology

At biological technical field, DNA is a requisite research point in biological study, by effectively can grasp biological hereditary property and rule to the research of DNA, therefore DNA extraction technology is widely used at biological technical field, conventional phenol-chloroform extraction process Isolation and purification DNA is a kind of comparatively common DNA extraction and purification technique, the laboratory test tube that application is conventional or centrifuge tube carry out conventional phenol-chloroform extraction process Isolation and purification DNA and there is a lot of inconvenience, likely because the situation that experimental result is polluted mutually appears in misoperation in leaching process.

Summary of the invention

For overcoming problems of the prior art, the utility model provides a kind of conventional phenol-chloroform extraction process Isolation and purification DNA test kit, the all reagent set used by conventional phenol-chloroform extraction process Isolation and purification DNA are in a test kit, concentrate and carry out experimental implementation, described test kit entirety all manufactures with synthetic glass, Reagent Tube pipe shaft indicates scale, handled easily person observation experiment data, drip experimental solutions, provide conveniently for operator carries out test operation, described Reagent Tube is fixed on test kit inside by bracing frame and base, effectively prevent the situation generation that Reagent Tube tilts to cause solvent to reveal, improve extraction efficiency.

For achieving the above object, the utility model is achieved by the following technical solution: the primary structure of described conventional phenol-chloroform extraction process Isolation and purification DNA test kit comprises box body, lid, lid switch, the saturated phenol reagent pipe of Tris, the saturated phenol solution Reagent Tube of ethanol, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube, chloroform: primary isoamyl alcohol (24:1) mixed solution Reagent Tube, without water-ice ethanol reagent pipe, 70% dehydrated alcohol Reagent Tube, TE buffering agents pipe, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube, ethanolic soln Reagent Tube, pipe lid, bracing frame and base, described box body is the structure of container of square, described lid and box body side are hinge engaging structure, described lid switch is positioned on box body, and described nine Reagent Tubes are cylindrical structure, and described pipe lid is connected with the Reagent Tube mouth of pipe, support frame as described above is positioned at tray interior, surface is provided with the aperture corresponding with nine Reagent Tubes, and described base is positioned at bottom test kit, and susceptor surface is provided with the groove corresponding with Reagent Tube.

Box body in described test kit, lid, Reagent Tube, bracing frame and base, be water white synthetic glass and make.

Described test kit is also provided with base except bracing frame, and susceptor surface is provided with the groove corresponding with Reagent Tube size.

Described Reagent Tube pipe shaft is marked with scale, and scale is accurate to μ l, adopts the mode be threaded to connect between reagent tube and tube lid.

The beneficial effects of the utility model: the utility model structure is simple, easy to operate, concentrate all experimental implementation, avoid the generation of experiment error, improve conventional efficient.

Accompanying drawing explanation

Fig. 1 is one-piece construction schematic diagram of the present utility model;

Fig. 2 is the structural representation of Reagent Tube;

Fig. 3 is the cross-sectional view of kit;

In figure, 1-box body, 2-lid, 3-lid switch, 4-bracing frame, 5-Tris saturated phenol reagent pipe, the saturated phenol solution Reagent Tube of 6-ethanol, 7-phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (a), 8-chloroform: primary isoamyl alcohol (24:1) mixed solution Reagent Tube, 9-are without water-ice ethanol reagent pipe, 10-70% dehydrated alcohol Reagent Tube, 11-TE buffering agents pipe, 12-phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (b), 13-ethanolic soln Reagent Tube, 14-pipe lid, 15-base.

Embodiment

In order to make the purpose of this utility model, technical scheme and beneficial effect clearly, below in conjunction with accompanying drawing, the utility model is elaborated.

As shown in Figure 1, described box body 1 is the structure of container of a square, described lid 2 is connected with inside box body 1, described lid switch 3 is positioned on box body 1, it is inner that support frame as described above 4 is positioned at box body 1, surface has the hole corresponding with Reagent Tube, the saturated phenol reagent pipe 5 of described Tris, the saturated phenol solution Reagent Tube 6 of ethanol, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (a) 7, chloroform: primary isoamyl alcohol (24:1) mixed solution Reagent Tube 8, without water-ice ethanol reagent pipe 9, 70% dehydrated alcohol Reagent Tube 10, TE buffering agents pipe 11, for extracting DNA, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube ((b) 12, ethanolic soln Reagent Tube 13, be positioned at the aperture on bracing frame 4 surface, box body 1, lid 2, bracing frame 4, base 15 is made by colourless synthetic glass, be convenient to the observation of operation and the data of testing.

As shown in Figure 2, described Reagent Tube is cylindrical structure, and described pipe lid 14 and the Reagent Tube mouth of pipe adopt the mode be threaded to be connected.

As shown in Figure 3, it is inner that support frame as described above 4 is positioned at box body 1, and described base 15 is positioned at bottom box body 1, and base 15 surface is provided with the groove corresponding with Reagent Tube, effectively fixes Reagent Tube, prevents Reagent Tube run-off the straight in experimentation, and test solvent leaks.

When using the utility model to carry out the Isolation and purification of DNA, open pipe lid 14, add the saturated phenol reagent pipe 5 with Tris respectively successively, the saturated phenol solution Reagent Tube 6 of ethanol, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (a) 7, chloroform: primary isoamyl alcohol (24:1) mixed solution Reagent Tube 8, without water-ice ethanol reagent pipe 9, 70% dehydrated alcohol Reagent Tube 10, TE buffering agents pipe 11, for extracting DNA, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (b) 12, ethanolic soln Reagent Tube 13, corresponding solution, after in the saturated phenol reagent pipe 5 of Tris, add the biological tissue extracted liquid after protease treatment, after the operation steps of conveniently phenol-chloroform extraction process carry out experimental implementation, Isolation and purification is carried out to DNA.

The utility model structure is simple, easy to operate, effectively prevent the generation of slipping up in experimental implementation, experimental implementation is carried out by test kit, improve conventional efficient, test kit entirety is water white synthetic glass and makes, and facilitates the observation of data logging in experimentation and experimental phenomena.

What finally illustrate is, above preferred embodiment is only unrestricted for illustration of the technical solution of the utility model, although be described in detail the utility model by above preferred embodiment, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from the utility model claims limited range.

Claims

1. a conventional phenol-chloroform extraction process Isolation and purification DNA test kit, is characterized in that: the primary structure of described conventional phenol-chloroform extraction process Isolation and purification DNA test kit comprises box body, lid, lid switch, the saturated phenol reagent pipe of Tris, the saturated phenol solution Reagent Tube of ethanol, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (a), chloroform: primary isoamyl alcohol (24:1) mixed solution Reagent Tube, without water-ice ethanol reagent pipe, 70% dehydrated alcohol Reagent Tube, TE buffering agents pipe, phenol: chloroform: primary isoamyl alcohol (25:24:1) mixed solution Reagent Tube (b), ethanolic soln Reagent Tube, pipe lid, bracing frame and base, described box body is the structure of container of square, described lid and box body side are hinge engaging structure, described lid switch is positioned on box body, and described nine Reagent Tubes are cylindrical structure, and described pipe lid is connected with the Reagent Tube mouth of pipe, support frame as described above is positioned at tray interior, surface is provided with the aperture corresponding with nine Reagent Tubes, and described base is positioned at bottom test kit, and susceptor surface is provided with the groove corresponding with Reagent Tube.

2. one according to claim 1 conventional phenol-chloroform extraction process Isolation and purification DNA test kit, is characterized in that: the box body in described test kit, lid, Reagent Tube, bracing frame and base, is water white synthetic glass and makes.

3. one according to claim 1 conventional phenol-chloroform extraction process Isolation and purification DNA test kit, it is characterized in that: described test kit is also provided with base except bracing frame, susceptor surface is provided with the groove corresponding with Reagent Tube size.

4. one according to claim 1 conventional phenol-chloroform extraction process Isolation and purification DNA test kit,

It is characterized in that: described Reagent Tube pipe shaft is marked with scale, and scale is accurate to μ l, between reagent tube and tube lid, adopt the mode be threaded to connect.