CN204251605U - A kind of cell cultures sedimentation device - Google Patents
A kind of cell cultures sedimentation device Download PDFInfo
- Publication number
- CN204251605U CN204251605U CN201420705933.6U CN201420705933U CN204251605U CN 204251605 U CN204251605 U CN 204251605U CN 201420705933 U CN201420705933 U CN 201420705933U CN 204251605 U CN204251605 U CN 204251605U
- Authority
- CN
- China
- Prior art keywords
- outer tube
- sedimentation
- adapter cavity
- tank body
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of cell cultures sedimentation device, comprise sedimentation chamber and adapter cavity, be provided with sedimentation unit in sedimentation chamber, sedimentation chamber top is provided with liquid collecting tube, and adapter cavity is connected to the bottom of sedimentation chamber, the latus rectum of adapter cavity reduces from top to bottom, adapter cavity lower end is connected with outer tube, is provided with interior pipe in outer tube, and the lower end of outer tube is arranged with end pipe, the lower end of end pipe is provided with an outlet, and the axis of outlet and the axis of outer tube are the angle between 100 ~ 170 degree.The utility model utilizes the pressure difference between two entrances of double-jacket tube to form negative pressure in an ingress wherein, negative pressure is utilized to make liquid continuous circulation between double-jacket tube, in continuous circulation process, got back under gravity in tank body by the cell of sedimentation unit sedimentation in sedimentation chamber or microcarrier, realize settlement separate.The utility model can be arranged on cover top or side, makes amplification easy, and can carry liquid under without extra ducts and pumping section condition, make autoclave sterilization process in place convenient and reliable simultaneously.
Description
Technical field:
The utility model relates to mechanical field, particularly relates to the bio-mechanical for culturing cell, particularly a kind of cell cultures sedimentation device.
Background technology:
Animal cell culture technology is widely used in the research and production of biomedicine field.In order to improve cell density, needing to add substratum in culturing process, and staying in tank after cell is separated by certain device, because zooblast does not have the protection of cytolemma, very sensitive to external force, therefore can not adopt centrifugal method to be separated.In prior art, free cell cultures adopts rotary screen, but rotating wire Netease is blocked by cell; Micro-carriers cell culture often adopts settling leg, but settling leg is placed into after in stainless steel cell tank, is difficult to amplify.
Utility model content:
The purpose of this utility model is to provide a kind of cell cultures sedimentation device, and described this cell cultures sedimentation device will solve in prior art and be placed into for the settling leg of cultivating zooblast the technical problem being difficult to after in stainless steel cell tank amplify.
This cell cultures sedimentation device of the present utility model, comprise a container, wherein, a sedimentation chamber and an adapter cavity is included in described container, sedimentation unit is provided with in described sedimentation chamber, the top of container is provided with a liquid collecting tube, described liquid collecting tube is communicated with the top of sedimentation chamber, described adapter cavity is connected to the bottom of sedimentation chamber, the latus rectum of adapter cavity reduces from top to bottom, the lower end of container is connected with an outer tube, the upper end of described outer tube is communicated with adapter cavity, be provided with in outer tube in one and manage, gap is provided with between the outer wall of described interior pipe and the inwall of outer tube, the upper end of interior pipe extends to the bottom of adapter cavity and is communicated with adapter cavity, after the lower ends bent of interior pipe along outer tube radial direction extend and opening in the lower sides of outer tube, the lower end periphery of outer tube is arranged with an end pipe, the internal diameter of described end pipe is greater than the external diameter of outer tube lower end, sealing member is provided with between the inwall of end pipe upper end and the outer wall of outer tube lower end, the lower end of end pipe is provided with an outlet, the axis of described outlet and the axis of outer tube are the angle between 100 ~ 170 degree.
Further, described sedimentation unit is swash plate or inclined tube, and described swash plate or the bearing of trend of inclined tube are horizontal by the angle of 40 ~ 80 degree.
Further, sedimentation chamber axially horizontal by the angle of 40 ~ 80 degree.
Further, the bearing of trend of swash plate or inclined tube is horizontal by the angle of 60 degree.
Further, outer tube is fixedly installed in the top cover of a tank body, and the outer wall of outer tube is connected with the top cover of described tank body, and container is positioned at the top of tank body top cover.
Or outer tube is fixedly installed in the sidewall of a tank body, the outer wall of outer tube is connected with the sidewall of described tank body, and the axis of outer tube and the axis of tank body are the angle of 10 ~ 50 degree, and container is positioned at the top of the outside of tank body, outer tube and tank body connection section.
Further, end pipe has bending between 100 ~ 170 degree in the axial direction.
Or the side of the bottom of end pipe is provided with the inwall of inclination, the bearing of trend of the inwall of described inclination is crossing with the axis of end pipe.
Principle of work of the present utility model is: the lower end of outer tube extends to below the liquid level of nutrient solution in tank body, and the lower end outlet of interior pipe and end Guan Jun are immersed in nutrient solution.Receipts liquid pump is utilized to extract nutrient solution on liquid collecting tube, nutrient solution is transported in adapter cavity via outer tube and interior pipe, and constantly rises in sedimentation chamber from adapter cavity, due to the effect of gravity, cell in nutrient solution or microcarrier realize settlement separate in sedimentation chamber, are got back to adapter cavity by the particle of sedimentation.Meanwhile, produce negative pressure under the effect that the opening part of end pipe flows at tank inner fluid, after the liquid level in adapter cavity exceedes the top of interior pipe, liquid is continuous circulation between outer tube and interior pipe.Concrete, liquid flows to from the lower end outlet of interior pipe, rises in adapter cavity through interior pipe, liquid and by the cell of sedimentation or microcarrier by the gap between outer tube and interior pipe, the opening via end pipe turns back in tank.Liquid cleans outer tube and interior pipe simultaneously.
The utility model is compared with prior art, and its effect is actively with obvious.The utility model is provided with sedimentation chamber and adapter cavity in container, double-jacket tube is communicated with adapter cavity, two entrances are set in the bottom of double-jacket tube, the pressure difference between two entrances is utilized to form negative pressure in an ingress wherein, negative pressure is utilized to make liquid continuous circulation between double-jacket tube, in continuous circulation process, got back under gravity in tank body by the cell of sedimentation unit sedimentation in sedimentation chamber or microcarrier, realize settlement separate.The utility model easily can be arranged on the top of cover or the side of tank body, makes amplification become easy, and can carry liquid under without extra ducts and pumping section condition, makes autoclave sterilization process in place convenient and reliable simultaneously.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of an embodiment of a kind of cell cultures sedimentation device of the present utility model.
Fig. 2 is the enlarged view in A portion in Fig. 1.
Fig. 3 is the structural representation of another embodiment of a kind of cell cultures sedimentation device of the present utility model.
Fig. 4 is the enlarged view in B portion in Fig. 3.
Embodiment:
Embodiment 1:
As shown in Fig. 1 and Fig. 2, a kind of cell cultures sedimentation device of the present utility model, comprise a container 1, wherein, a sedimentation chamber 2 and an adapter cavity 3 is included in container 1, sedimentation unit 4 is provided with in sedimentation chamber 2, the top of container 1 is provided with a liquid collecting tube 5, liquid collecting tube 5 is communicated with the top of sedimentation chamber 2, adapter cavity 3 is connected to the bottom of sedimentation chamber 2, the latus rectum of adapter cavity 3 reduces from top to bottom, the lower end of container 1 is connected with an outer tube 6, the upper end of outer tube 6 is communicated with adapter cavity 3, an interior pipe 7 is provided with in outer tube 6, gap is provided with between the outer wall of interior pipe 7 and the inwall of outer tube 6, the upper end of interior pipe 7 extends to the bottom of adapter cavity 3 and is communicated with adapter cavity 3, after the lower ends bent of interior pipe 7 along outer tube 6 radial direction extend and opening in the lower sides of outer tube 6, the lower end periphery of outer tube 6 is arranged with an end pipe 8, the internal diameter of end pipe 8 is greater than the external diameter of outer tube 6 lower end, sealing member 9 is provided with between the inwall of end pipe 8 upper end and the outer wall of outer tube 6 lower end, the lower end of end pipe 8 is provided with an outlet 17, the axis of outlet and the axis of outer tube 6 are the angle between 100 ~ 170 degree.
Further, sedimentation unit 4 is swash plate or inclined tube, and the bearing of trend of swash plate or inclined tube is horizontal by the angle of 40 ~ 80 degree.
Further, sedimentation chamber 2 axially horizontal by the angle of 40 ~ 80 degree.
Further, the bearing of trend of swash plate or inclined tube is horizontal by the angle of 60 degree.
Further, outer tube 6 is fixedly installed in the top cover 11 of a tank body, and the outer wall of outer tube 6 is connected with the top cover 11 of tank body, and container 1 is positioned at the top of tank body top cover 11.
Further, the side of the bottom of end pipe 8 is provided with the inwall 16 of inclination, and the bearing of trend of the inwall of inclination is crossing with the axis of end pipe 8.
The specific works principle of embodiment 1 is: outer tube 6 is fixed in the top cover 11 of a tank body, receive liquid pump the nutrient solution 15 first flow speed in tank, be transported in adapter cavity 3 via outer tube 6 and interior pipe 7, the inwall of the inclination of the bottom of end pipe 8 produces negative pressure at the lower part outlet place of end pipe 8, when the liquid level 14 in adapter cavity 3 exceedes the top of interior pipe 7, liquid is continuous circulation between outer tube 6 and interior pipe 7, liquid flows to from the lower end outlet 13 of interior pipe 7 with the 3rd flow, be promoted in adapter cavity 3 through interior pipe 7, liquid and by the cell of sedimentation or microcarrier then by the gap between outer tube 6 and interior pipe 7, turn back in tank body with the lower part outlet of the second flow via end pipe 8.Liquid level constantly rises in sedimentation chamber 2 from adapter cavity 3, and due to the effect of gravity, the cell in nutrient solution or microcarrier realize settlement separate at sedimentation chamber 2, by the particle of sedimentation, through adapter cavity 3, then by outer tube 6, return tank body from the lower part outlet of end pipe 8, clean outer tube 6 and interior pipe 7 simultaneously.
Embodiment 2:
As shown in Fig. 3 and Fig. 4, outer tube 6 is fixedly installed in the sidewall of a tank body 12, the outer wall of outer tube 6 is connected with the sidewall of tank body 12, and the axis of outer tube 6 and the axis of tank body 12 are the angle of 10 ~ 50 degree, and container 1 is positioned at the top of the outside of tank body 12, outer tube 6 and tank body 12 connection section.
Further, end pipe 8 has bending between 100 ~ 170 degree in the axial direction.
The specific works principle of embodiment 2 is: outer tube 6 is fixed on the side of tank body 12.Receiving liquid pump rises to the nutrient solution 15 in tank in adapter cavity 3 via end pipe 8, outer tube 6 and interior pipe 7, under the effect on inclined-plane, end pipe 8 exit, produces negative pressure in end pipe 8 exit.When the liquid level 14 in adapter cavity 3 exceedes the top of interior pipe 7, liquid circulates continuously between outer tube 6 and interior pipe 7.Now liquid sucks from the lower end of interior pipe 7 outlet 13, and liquid and being exported from end pipe 8 via the gap between outer tube 6 and interior pipe 7 by sedimentation cell or microcarrier turns back to tank body 12.The liquid of continuous rising enters in sedimentation chamber 2 from adapter cavity 3, and cell or microcarrier are then got back in tank via outer tube 6 and end pipe 8, and settling particle is not only transmitted back in tank by this backflow, also plays the effect of constantly cleaning outer tube 6 and interior pipe 7.
Above embodiment is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, do not departing under prerequisite of the present utility model; can also make and improve and modify, these improve and modify and also should be considered as protection domain of the present utility model.
Claims (8)
1. a cell cultures sedimentation device, comprise a container, it is characterized in that: in described container, include a sedimentation chamber and an adapter cavity, sedimentation unit is provided with in described sedimentation chamber, the top of container is provided with a liquid collecting tube, described liquid collecting tube is communicated with the top of sedimentation chamber, described adapter cavity is connected to the bottom of sedimentation chamber, the latus rectum of adapter cavity reduces from top to bottom, the lower end of container is connected with an outer tube, the upper end of described outer tube is communicated with adapter cavity, be provided with in outer tube in one and manage, gap is provided with between the outer wall of described interior pipe and the inwall of outer tube, the upper end of interior pipe extends to the bottom of adapter cavity and is communicated with adapter cavity, after the lower ends bent of interior pipe along outer tube radial direction extend and opening in the lower sides of outer tube, the lower end periphery of outer tube is arranged with an end pipe, the internal diameter of described end pipe is greater than the external diameter of outer tube lower end, sealing member is provided with between the inwall of end pipe upper end and the outer wall of outer tube lower end, the lower end of end pipe is provided with an outlet, the axis of described outlet and the axis of outer tube are the angle between 100 ~ 170 degree.
2. a kind of cell cultures sedimentation device as claimed in claim 1, is characterized in that: described sedimentation unit is swash plate or inclined tube, and described swash plate or the bearing of trend of inclined tube are horizontal by the angle of 40 ~ 80 degree.
3. a kind of cell cultures sedimentation device as claimed in claim 1, is characterized in that: sedimentation chamber axially horizontal by the angle of 40 ~ 80 degree.
4. a kind of cell cultures sedimentation device as claimed in claim 2, is characterized in that: the bearing of trend of swash plate or inclined tube is horizontal by the angle of 60 degree.
5. a kind of cell cultures sedimentation device as claimed in claim 1, it is characterized in that: outer tube is fixedly installed in the top cover of a tank body, the outer wall of outer tube is connected with the top cover of described tank body, and container is positioned at the top of tank body top cover.
6. a kind of cell cultures sedimentation device as claimed in claim 1, it is characterized in that: outer tube is fixedly installed in the sidewall of a tank body, the outer wall of outer tube is connected with the sidewall of described tank body, the axis of outer tube and the axis of tank body are the angle of 10 ~ 50 degree, and container is positioned at the top of the outside of tank body, outer tube and tank body connection section.
7. a kind of cell cultures sedimentation device as claimed in claim 1, is characterized in that: end pipe has bending between 100 ~ 170 degree in the axial direction.
8. a kind of cell cultures sedimentation device as claimed in claim 1, it is characterized in that: the side of the bottom of end pipe is provided with the inwall of inclination, the bearing of trend of the inwall of described inclination is crossing with the axis of end pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420705933.6U CN204251605U (en) | 2014-11-21 | 2014-11-21 | A kind of cell cultures sedimentation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420705933.6U CN204251605U (en) | 2014-11-21 | 2014-11-21 | A kind of cell cultures sedimentation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204251605U true CN204251605U (en) | 2015-04-08 |
Family
ID=52955930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420705933.6U Withdrawn - After Issue CN204251605U (en) | 2014-11-21 | 2014-11-21 | A kind of cell cultures sedimentation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204251605U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105602825A (en) * | 2014-11-21 | 2016-05-25 | 上海保兴生物设备工程有限公司 | Cell culture sedimentation device |
-
2014
- 2014-11-21 CN CN201420705933.6U patent/CN204251605U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105602825A (en) * | 2014-11-21 | 2016-05-25 | 上海保兴生物设备工程有限公司 | Cell culture sedimentation device |
| CN105602825B (en) * | 2014-11-21 | 2018-12-07 | 上海保兴生物设备工程有限公司 | Cell culture sedimentation device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204275826U (en) | Hydraulic automatism dosing agitating device | |
| CN204251605U (en) | A kind of cell cultures sedimentation device | |
| CN203976496U (en) | A kind of full-automatic oxygenation ecosystem fast for water treatment | |
| CN203750210U (en) | Traditional Chinese medicine digestion device | |
| CN205013396U (en) | Air lift | |
| CN204502536U (en) | A kind of food garbage equipment for separating liquid from solid | |
| CN203212379U (en) | Pulse water distributor | |
| CN105602825B (en) | Cell culture sedimentation device | |
| CN207885501U (en) | A kind of South America shrimp culture pond picotan | |
| CN103382436A (en) | Continuous perfusion device of fermentation tank | |
| CN209525136U (en) | A kind of hybrid synchronous sampling device of sewage | |
| CN203451520U (en) | Continuous injection device of fermentation tank | |
| CN204602049U (en) | Ph value regulating tank | |
| CN204275652U (en) | A kind of cutting fluid cleaning plant processing iron product | |
| CN205288563U (en) | Liquid material crushing device | |
| CN205340658U (en) | Automatic dosing device | |
| CN204174193U (en) | Yellow water stream feeder apparatus and yellow water stream adding system | |
| CN205170721U (en) | Liquaemin filter equipment | |
| CN203820795U (en) | Siphoning type high-density cell culture flask | |
| CN107754379A (en) | A kind of liquid fertilizer vacuum deaerator plant | |
| CN201729708U (en) | Bag-type sewage treatment hydrolysis acidification device | |
| CN212356673U (en) | Medicine adding system | |
| CN202438175U (en) | Self-cleaning extraction tank | |
| CN104383723A (en) | Sewage treatment unit | |
| CN105385565A (en) | Improved bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20150408 Effective date of abandoning: 20181207 |
|
| AV01 | Patent right actively abandoned |