CN204241488U - Pepsinogen I II two-in-one collaurum quantitative testing test paper bar and apply its device - Google Patents
Pepsinogen I II two-in-one collaurum quantitative testing test paper bar and apply its device Download PDFInfo
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- CN204241488U CN204241488U CN201420795443.XU CN201420795443U CN204241488U CN 204241488 U CN204241488 U CN 204241488U CN 201420795443 U CN201420795443 U CN 201420795443U CN 204241488 U CN204241488 U CN 204241488U
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- pepsinogen cgene
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Abstract
The utility model provides a kind of pepsinogen Cgene/II (PG I/II) two-in-one collaurum quantitative testing test paper bar and applies its pick-up unit, described test strips comprises backboard, and along thieving paper, nitrocellulose filter, gold mark pad and sample pad that described test strips length direction covers in turn on described backboard; Described nitrocellulose filter covers the middle part in described backboard, and two detection zone be provided with separately and a quality control band, described detection zone is near described monoclonal antibody pad, and described quality control band is near described thieving paper; Described gold mark pad is for being coated with the polyester film of the monoclonal antibody of colloid gold label.Described pepsinogen Cgene/II (PG I/II) two-in-one collaurum quantitative testing test paper bar can realize quick, single part and quantitatively detect, and specificity is good, highly sensitive, batch in, batch between error little, greatly facilitate clinical practice.
Description
Technical field
The utility model relates to clinical detection field, is specifically related to the device that one fast, quantitatively detects pepsinogen Cgene/II (PG I/II) two-in-one collaurum quantitative testing test paper bar and applies it.
Background technology
Propepsin (PG) can be divided into PG I and PG II two kinds of hypotypes by the protease precursor of gastric secretion.PG I derives from chief cell and the mucous neck cell of fundus gland, and PG I is the pointer detecting oxyntic gland (fundus gland) cell function, and gastric acid secretion increases PG I rising, and gastric acid secretion minimizing or gastric mucosa body of gland atrophy PG I reduce; PG II derives from full gastric gland, duodenum, prostate and pancreas also produce a small amount of PG II, the correlativity comparatively large (relative to antrum) of PG II and gastric mucosa pathology, it raises relevant with fundus gland shrink tube, intestinal metaplasia or Pseudopyloric gland metaplasia, dysplasia; The reduction of PG I/II ratio Progressive symmetric erythrokeratodermia is in progress relevant to atrophy of gastric mucosa.
Summary of the invention
To be solved in the utility model is that the detection method of pepsinogen Cgene/II in prior art can not take into account highly sensitive, high specificity and technical matters simple to operate, a kind of simple to operate, high specificity, highly sensitive is provided, fast, quantitatively can detects the colloidal gold immuno-chromatography test paper strip of pepsinogen Cgene.
The purpose of this utility model is achieved through the following technical solutions:
The two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II described in the utility model (PG I/II), comprise: backboard, and the thieving paper covered in turn on described backboard along described carapace length direction, nitrocellulose filter, gold mark pad and sample pad; Wherein, described nitrocellulose filter covers the middle part in described backboard, and be provided with separately be coated with pepsinogen Cgene monoclonal antibody, PGⅡ monoclonal antibody two detection zone and be coated with a quality control band of rabbit against murine lgG antibody, quality control band is near described thieving paper; Described gold mark pad is the polyester film of the collaurum being coated with surface indicia pepsinogen Cgene monoclonal antibody and PGⅡ monoclonal antibody; Described thieving paper, described nitrocellulose filter, described gold mark pad and described sample pad between, successively with and only contact with adjacent regions and partly overlap.
Further, the particle diameter of described collaurum is 520 nm ~ 525nm.
Further, the width of described test strips is 0.25cm ~ 1.00cm.
Further, between described thieving paper, described nitrocellulose filter, described gold mark pad and described sample pad, successively with and the overlap length in the region that only partly overlaps with adjacent regions is independent of each other for 1mm ~ 5mm.
Further, partly overlap described in described thieving paper and described nitrocellulose filter the upside of region at described cellulose nitrate coated film.
Further, described backboard is PVC backboard.
Another object of the present utility model is achieved through the following technical solutions:
Pepsinogen Cgene albumen described in the utility model (the white proenzyme of PG) two-in-one collaurum quantitative testing test paper bar comprises test strip and wraps up the jam of described test strips; Described jam comprises panel and base plate further, described panel is provided with view window and loading slot; Described loading slot is opened on described sample pad top, with exposed portion or whole described sample pad; Described view window is opened on the upside of described coated film, to expose whole described detection zone and described quality control band.
Further, described panel and base plate adopt the acrylonitrile-butadiene-styrene materials not producing magnetic and fluorescent effect to make.
the utility model beneficial effect is compared to existing technology:
1, pepsinogen Cgene albumen provided by the utility model (the white proenzyme of PG) two-in-one collaurum quantitative testing test paper bar take collaurum as signal source, be convenient to the gathering of labeled molecule at detection zone and quality control band place, play the effect that signal amplifies, quick, single part can be realized quantitatively detect, and highly sensitive, high specificity, for Clinical practice provides a great convenience;
2, pepsinogen Cgene albumen provided by the utility model (the white proenzyme of PG) two-in-one collaurum quantitative testing test paper bar, easy and simple to handle, volume is little, be easy to carry, and is easy to preserve, and is conducive to application and the popularization of basic hospital;
3, pepsinogen Cgene albumen provided by the utility model (the white proenzyme of PG) two-in-one collaurum quantitative testing test paper bar, technology maturation, preparation method is simple, can realize batch production.
Accompanying drawing explanation
In order to make content of the present utility model be more likely to be clearly understood, below according to specific embodiment of the utility model also by reference to the accompanying drawings, the utility model is described in further detail, wherein:
Fig. 1 is the structural representation of pepsinogen Cgene albumen described in the utility model (the white proenzyme of PG) two-in-one collaurum quantitative testing device;
Fig. 2 is the front view that pepsinogen Cgene albumen described in the utility model (the white proenzyme of PG) two-in-one collaurum quantitative testing test paper bar is arranged on base plate;
Fig. 3 is the side view of test strips described in the utility model;
In figure, Reference numeral is expressed as: 1. base plate, 2. panel, 3. test strips, 4. backboard, 5. sample pad, 6. gold mark pad, 7. nitrocellulose filter, 8. thieving paper, 9. detection zone, 10. view window, 11. quality control bands, 12. loading slots.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly, below in conjunction with accompanying drawing, embodiment of the present utility model is described in further detail.
The utility model can be implemented in many different forms, and should not be understood to be limited to embodiment set forth herein.On the contrary, provide these embodiments, making the utility model open will be thorough and complete, and design of the present utility model fully will be conveyed to those skilled in the art, and the utility model will only be limited by claim.In the accompanying drawings, for clarity, the size in layer and region and relative size can be exaggerated.
In following embodiment, described pepsinogen Cgene monoclonal antibody, PGⅡ monoclonal antibody, rabbit against murine lgG antibody, phosphate buffer solution, bovine serum albumin(BSA) (BSA), sucrose, polyester film, nitrocellulose filter, glass fibre element film, high-intensity water absorbent paper are commercially available.
embodiment 1
As Figure 1-3, the utility model provides a kind of pepsinogen Cgene albumen (the white proenzyme of PG) two-in-one collaurum quantitative testing test paper bar 3, described test strips comprises backboard 4, and along thieving paper 8, nitrocellulose filter 7, gold mark pad 6 and sample pad 5 that described test strips length direction covers in turn on described backboard.
Described nitrocellulose filter covers the middle part in described backboard, and the detection zone be provided with separately and quality control band, described detection zone is near described gold mark pad, and described quality control band is near described thieving paper.
Described gold mark pad is the polyester film of the collaurum being coated with finishing pepsinogen Cgene monoclonal antibody and PGⅡ monoclonal antibody.
Described sample pad is glass fibre element film.
Described detection zone is coated on pepsinogen Cgene monoclonal antibody band on described nitrocellulose filter and PGⅡ monoclonal antibody band, and quality control band is the rabbit against murine lgG antibody band be coated on described nitrocellulose filter.
Described thieving paper, described nitrocellulose filter, described gold mark pad and described sample pad between, successively with and only contact with adjacent regions and partly overlap.Between described thieving paper, described nitrocellulose filter, described gold mark pad and described sample pad, successively with and the overlap length in the region that only partly overlaps with adjacent regions is independent of each other for 1mm ~ 5mm, be preferably 2.5nm.
The particle diameter of described collaurum is 520nm ~ 525nm, is preferably 525nm.
The width of described test strips is 0.25cm ~ 1.00cm, is preferably 0.4cm.
Described backboard is PVC backboard, and backboard carries adhesive sticker coating.
embodiment 2
The present embodiment provides a kind of and detects pepsinogen Cgene/confession collaurum quantitative testing device, comprises described test strips and wraps up the jam of described test strips; Described jam comprises base plate 1 and panel 2 further, described panel is provided with view window and loading slot, to expose the regional area of test strips; Described loading slot is opened on described sample pad top, with exposed portion or whole described sample pad region; Described view window is opened on the upside of described cellulose nitrate coated film, to expose all described two detection zone and described quality control band.
Described backboard is PVC offset plate.The particle diameter of described collaurum is 520 ~ 525nm.Described colloid gold label has mouse monoclonal antibody; Described thieving paper preferred high strength thieving paper; Described jam does not preferably produce the acrylonitrile-butadiene-styrene materials of magnetic and fluorescent effect.
During use, sample liquid is added drop-wise in sample pad 5 by loading slot 12, under capillary action, sample liquid is to thieving paper 8 side swimming, when containing pepsinogen Cgene and PGⅡ in sample liquid, pepsinogen Cgene and PGⅡ form antigen-antibody complex with the pepsinogen Cgene monoclonal antibody on collaurum and PGⅡ monoclonal antibody respectively, along with chromatography effect, described antigen-antibody complex continues to thieving paper 8 side swimming, arrive the pepsinogen Cgene monoclonal antibody and PGⅡ monoclonal antibody place that identify different epitope, i.e. two detection zone 9 places, form antibody-antigen-antibody compound, the aggregation with the collaurum of antibody-antigen-antibody compound is formed at two detection zone 9 places.And continue to thieving paper 8 side swimming in conjunction with the fluorescent microsphere of monoclonal antibody I, when arriving quality control band 11, the mouse monoclonal antibody of rabbit against murine lgG antibody on fluorescent microsphere is combined, and produces the gathering of collaurum at quality control band 11 place.
The fluorescence immune chromatography reagent card of detection pepsinogen Cgene/reality provided by the utility model can realize quick, single quantitative detection, and highly sensitive, batch in, batch between error little, for Clinical practice provides a great convenience; And volume is little, be easy to carry, is easy to preserve, is conducive to application and the popularization of basic hospital; In addition, preparation method is simple, technology maturation, can realize batch production.
Claims (8)
1. the two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II (PG I/II), comprise backboard (4), thieving paper (8), nitrocellulose filter (7), gold mark pad (6) and sample pad (5), it is characterized in that, described thieving paper, nitrocellulose filter, gold mark pad and sample pad cover on described backboard along carapace length direction in turn; Wherein, described nitrocellulose filter covers the middle part in described backboard, and be provided with the detection zone being coated with pepsinogen Cgene monoclonal antibody separately, PGⅡ monoclonal antibody detection zone and be coated with the quality control band (11) of rabbit against murine lgG antibody, described quality control band is near the side of described thieving paper, and described detection zone (9) is near the side of described gold mark pad; Described gold mark pad is the polyester film of the collaurum being coated with surface indicia pepsinogen Cgene monoclonal antibody and PGⅡ monoclonal antibody; Described thieving paper, described nitrocellulose filter, described gold mark pad and described sample pad between, successively with and only contact with adjacent regions and partly overlap.
2. the two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II according to claim 1 (PG I/II), is characterized in that, the particle diameter of described collaurum is 520 nm ~ 525nm.
3. the two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II according to claim 1 and 2 (PG I/II), is characterized in that, the width of described test strips is 0.25cm ~ 1.00cm.
4. the two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II according to claim 3 (PG I/II), it is characterized in that, between described thieving paper, described nitrocellulose filter, described gold mark pad and described sample pad, successively with and the overlap length in the region that only partly overlaps with adjacent regions is independent of each other for 1mm ~ 5mm.
5. the two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II according to claim 4 (PG I/II), it is characterized in that, partly overlap described in described thieving paper and described nitrocellulose filter the upside of region at described cellulose nitrate coated film.
6. the two-in-one collaurum quantitative testing test paper bar of pepsinogen Cgene/II according to claim 5 (PG I/II), it is characterized in that, described backboard is PVC backboard.
7. pepsinogen Cgene/II (PG I/II) two-in-one collaurum quantitative testing device, is characterized in that, comprises the described test strips of one of claim 1-6 and wraps up the jam of described test strips; Described jam comprises panel (2) and base plate (1) further, described panel is provided with view window (10) and loading slot (12); Described loading slot is opened on described sample pad top, with exposed portion or whole described sample pad; Described view window is opened on the upside of described coated film, to expose whole described detection zone and described quality control band.
8. pepsinogen Cgene/II according to claim 7 (PG I/II) two-in-one collaurum quantitative testing device, is characterized in that, described panel and base plate plate adopt acrylonitrile-butadiene-styrene materials to make.
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| CN201420795443.XU CN204241488U (en) | 2014-12-16 | 2014-12-16 | Pepsinogen I II two-in-one collaurum quantitative testing test paper bar and apply its device |
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| CN201420795443.XU CN204241488U (en) | 2014-12-16 | 2014-12-16 | Pepsinogen I II two-in-one collaurum quantitative testing test paper bar and apply its device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109557318A (en) * | 2019-01-31 | 2019-04-02 | 浙江理工大学 | M type phospholipase A2 receptor and 1 type thrombospondin 7A domain autoantibody immunochromatography item and detection card are detected simultaneously |
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2014
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109557318A (en) * | 2019-01-31 | 2019-04-02 | 浙江理工大学 | M type phospholipase A2 receptor and 1 type thrombospondin 7A domain autoantibody immunochromatography item and detection card are detected simultaneously |
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