CN204211746U - For the papery microfluid of isothermal duplication nucleic acid - Google Patents
For the papery microfluid of isothermal duplication nucleic acid Download PDFInfo
- Publication number
- CN204211746U CN204211746U CN201420583698.XU CN201420583698U CN204211746U CN 204211746 U CN204211746 U CN 204211746U CN 201420583698 U CN201420583698 U CN 201420583698U CN 204211746 U CN204211746 U CN 204211746U
- Authority
- CN
- China
- Prior art keywords
- layer
- sample
- application
- well
- diameter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of papery microfluid for isothermal duplication nucleic acid, comprise the responding layer be made up of filter paper and at least one deck application of sample layer, application of sample ply is placed on the top of responding layer, application of sample layer center is provided with well, application of sample layer is evenly equipped with multiple reacting hole around well, the aperture of well and reacting hole is coated with hydrophobic material; Responding layer is provided with the sample area corresponding with well drawn by hydrophobic material, the multiple reaction zone corresponding with reacting hole and reaction channel, reaction zone diameter is identical with the diameter of reacting hole, the diameter of sample area is less than the diameter of well, each reaction zone is communicated with sample area by a reaction channel, and the width of reaction channel diminishes gradually from reaction zone to sample area.Have produce simple, cost is low, single use avoids the features such as crossed contamination, multiple reacting hole makes it possible to the different test items simultaneously detecting same target.
Description
Technical field
The utility model relates to a kind of papery microfluid, particularly relates to a kind of papery microfluid for isothermal duplication nucleic acid, belongs to biology, analytical chemistry and medical science.
Background technology
All the time, a platform simple, quick, accurate and effective can carry out the common expectation that multi-channel detection is all circles scholars such as medical science, biochemistry, environment.Microfluidic chip technology (microfluidicstechnology) has efficiently, low consumption (comprising time, sample, reagent), analyze feature that is microminiaturized and experiment flux, and by conjunction with sensing or sample pre-treatments module, add the validity of analyzing and testing, decrease crossed contamination, for Site Detection or detection (point-of-care, POC) in time provide a kind of method of quick diagnosis.Constant temperature microfluid nucleic acid amplification chip technology is a kind of new technique judged for nucleic acid rapid amplifying and result naked eyes of a kind of novelty bind nucleic acid isothermal duplication and micro-fluidic chip.But microfluidic device generally adopts semi-conductor or unicircuit cooked mode to realize, and process is loaded down with trivial details, complex process, and cost still far can not reach disposable target so far.Therefore, those skilled in the art are devoted to look for that a kind of structure is simple, low cost of manufacture, easy to detect, disposable microfluid.
Utility model content
Because the above-mentioned defect of prior art, technical problem to be solved in the utility model is to provide a kind of papery microfluid for isothermal duplication nucleic acid, and structure is simple, low cost of manufacture, easy to detect, disposable.
The purpose of this utility model is achieved through the following technical solutions: a kind of papery microfluid for isothermal duplication nucleic acid, comprise the responding layer be made up of filter paper and at least one deck application of sample layer, described application of sample ply is placed on the top of responding layer, described application of sample layer center is provided with well, described application of sample layer is evenly equipped with multiple reacting hole around well, the aperture of described well and reacting hole is coated with hydrophobic material; Described responding layer is provided with the sample area corresponding with well drawn by hydrophobic material, the multiple reaction zone corresponding with reacting hole and reaction channel, described reaction zone diameter is identical with the diameter of reacting hole, the diameter of described sample area is less than the diameter of well, each reaction zone is communicated with sample area by a reaction channel, and the width of described reaction channel diminishes gradually from reaction zone to sample area.
Adopt technique scheme,
The aperture of well and reacting hole is coated with hydrophobic material, responding layer is provided with the sample area corresponding with well drawn by hydrophobic material, the multiple reaction zone corresponding with reacting hole and reaction channel, be not diffused into other place of filter paper layer by hydrophobic material after making testing sample solution be added drop-wise to sample area by well, thus the detection of disparity items is separated, a miniflow physical efficiency carries out multinomial different detection simultaneously.
In technique scheme: described application of sample layer be two-layer more than, the diameter of the well of application of sample layer successively reduces from top to bottom, and the diameter of described sample area is less than the diameter of the well of orlop application of sample layer.The diameter of well successively reduces, and makes the central authorities of microfluid form one from outer toward the diminishing hole of interior diameter, decreases sample and absorbed the loss brought by filter paper.
In technique scheme: the center of circle of all reacting holes is circumferentially same, and the center of circle of all reaction zones, also circumferentially same, makes microfluidic device beautiful and clean, result is easy to observe statistics.
In technique scheme: described hydrophobic material is paraffin or dimethyl siloxane or SU-8 photoresistance glue.
In technique scheme: described papery microfluid also comprises the zone of heating be positioned at below responding layer, described zone of heating is made up of filter paper, its lower surface is provided with resistance wire, can contact resistance, makes ply of paper temperature reach nucleic acid amplification reaction temperature required after making current.
In technique scheme: the resistance wire of described zone of heating is for be formed by metal powder spray precipitation, and resistance wire is fine, volume is little, can combine closely with filter paper layer, and be beneficial to be uniformly distributed and make ply of paper homogeneous temperature.
In technique scheme: described application of sample layer, responding layer and zone of heating are pasted superposition by layers of two-sided and fixed, between wherein said application of sample layer, application of sample layer and the layers of two-sided between responding layer be provided with and the well of the application of sample layer of the side of being located thereon and hole corresponding to reacting hole, make whole microfluidic structures firm, each layer can not be shifted and avoid causing experimental result inaccurate.
The beneficial effects of the utility model are: the papery microfluid for isothermal duplication nucleic acid that the utility model provides is prepared by paper, acted between paper fiber by capillary action and liquid wick and carry out horizontal and vertical flowing, testing sample can be reacted with the primer being coated on reaction zone and realize amplification object, have light, cheap, produce simple, single use and avoid the features such as crossed contamination, multiple reacting hole makes it possible to the different test items simultaneously detecting same target.This microfluid can access power supply by conductive layer from outside, ply of paper temperature is made to reach the needs of amplification temperature, can not want zone of heating directly to put into the artificial culture case mixing up temperature carry out amplified reaction by only assembling by application of sample layer and responding layer the microfluid obtained, simplify the preparation process of this microfluid, reduce cost yet.
Accompanying drawing explanation
Fig. 1 is the microfluid assembling schematic diagram of the utility model one embodiment.
Fig. 2 is the application of sample Rotating fields schematic diagram of the utility model one embodiment.
Fig. 3 is the responding layer structural representation of the utility model one embodiment.
Fig. 4 is the zone of heating structural representation of the utility model one embodiment.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail:
Embodiment 1:
As shown in figures 1-4, the papery microfluid for isothermal duplication nucleic acid of the present utility model stacks by least one deck application of sample layer 1, responding layer 2 and layers of two-sided 4 the papery microfluid forming isothermal duplication nucleic acid from top to bottom successively, selects two-layer application of sample layer 1 here.
From top to bottom: the first layer application of sample layer 1 is made up of filter paper, its center is provided with the well 11 that diameter is 10mm, the reacting hole 12 that 8 diameters are 5mm is laid with around well 11, reacting hole 12 apart from well 11 apart from being 5mm, the aperture of well 11 and reacting hole 12 be coated with hydrophobic material paraffin or dimethyl siloxane (polydimethylsiloxane, PDMS) or SU-8 photoresistance glue (SU-8photoresist) to make in hole liquid not by.
Second layer application of sample layer 1 is identical with the first layer structure, is also provided with well 11 and reacting hole 12, and the diameter of the well 11 of layer 2-only application of sample layer 1 is less than the diameter of upper strata application of sample layer 1, and the diameter being preferably the well 11 of second layer application of sample layer 1 is 9mm.
It is bonding that the first layer application of sample layer 1 and second layer application of sample layer 2 pass through layers of two-sided 4, and this layer of layers of two-sided 4 is provided with the hole corresponding with the well 11 of the application of sample layer 1 of the first layer and reacting hole 12.
By the bonding responding layer 2 of layers of two-sided 4 below second layer application of sample layer 1, this layer of layers of two-sided 4 is provided with the hole corresponding with the well 11 of the application of sample layer 1 of the second layer and reacting hole 12.
Responding layer 2 is made up of filter paper, responding layer 2 is provided with the sample area 21 corresponding with well 11 that go out by hydrophobic material paraffin or dimethyl siloxane or SU-8 photoresistance tempera painting and 8 corresponding reaction zones 22 and reaction channel 23 with reacting hole 12, sample area diameter is 6 ~ 10mm, reaction zone diameter is 5mm, each reaction zone 22 is communicated with sample area 21 by a reaction channel 23, and the width of reaction channel 23 diminishes from reaction zone 22 gradually to sample area 21.Reaction zone 22 is coated with the primer corresponding with detected object, and primer is combined by biotinylated primer and the coupling of avidin microgel, and drying at room temperature is deposited in reaction zone 22.
During use, the testing sample reaction solution being mixed with metallochromic indicator is dripped in well 11, papery microfluid covers one deck heat resistant plastice film and prevents volatilization, then microfluid is placed in the artificial culture case mixing up temperature in advance, testing sample reaction solution is acted on by capillary action and liquid wick and to carry out horizontal and vertical flowing between paper fiber and arrive reaction zone 22 and start isothermal nucleic acid amplification with primer specific identification and react, and reacting the colour-change terminating rear observing response district can obtain detected result.
Embodiment 2: other is identical with embodiment 1, unlike: be pasted with zone of heating 3 in responding layer 2 below by layers of two-sided 4, without any pattern and hole in this layers of two-sided 4.Zone of heating 3 is made up of filter paper, and its lower surface is provided with resistance wire 31, and be preferably resistance wire 31 and formed by metal powder spray precipitation, resistance wire 31 is connected with switch with power supply by wire after connecting with variable resistor 5.Changed the size of electric current by the adjustment of variable resistor 5, thus play the effect of the temperature regulating zone of heating 3.During use, the testing sample being mixed with metallochromic indicator is dripped in well 11, papery microfluid covers one deck heat resistant plastice film and prevents volatilization, switch on power, regulate variable resistor 5, make micro-fluidic temperature reach amplified reaction temperature required, the colour-change that reaction terminates rear observing response district can obtain detected result.
More than describe preferred embodiment of the present utility model in detail.Should be appreciated that those of ordinary skill in the art just can make many modifications and variations according to design of the present utility model without the need to creative work.Therefore, all technician in the art according to design of the present utility model on the basis of existing technology by the available technical scheme of logical analysis, reasoning, or a limited experiment, all should by the determined protection domain of claims.
Claims (7)
1. the papery microfluid for isothermal duplication nucleic acid, it is characterized in that: comprise the responding layer (2) be made up of filter paper and at least one deck application of sample layer (1), described application of sample layer (1) overlap is placed on the top of responding layer (2), described application of sample layer (1) center is provided with well (11), described application of sample layer (1) is evenly equipped with multiple reacting hole (12) around well (11), the aperture of described well (11) and reacting hole (12) is coated with hydrophobic material, described responding layer (2) is provided with the sample area (21) corresponding with well (11) drawn by hydrophobic material, the multiple reaction zones (22) corresponding with reacting hole (12) and reaction channel (23), described reaction zone (22) diameter is identical with the diameter of reacting hole (12), the diameter of described sample area (21) is less than the diameter of well (11), each reaction zone (22) is communicated with sample area (21) by a reaction channel (23), the width of described reaction channel (23) diminishes from reaction zone (22) gradually to sample area (21).
2. the papery microfluid for isothermal duplication nucleic acid according to claim 1, it is characterized in that: described application of sample layer (1) is for more than two-layer, the diameter of the well (11) of application of sample layer (1) successively reduces from top to bottom, and the diameter of described sample area (21) is less than the diameter of the well (11) of orlop application of sample layer (1).
3. the papery microfluid for isothermal duplication nucleic acid according to claim 1 and 2, is characterized in that: the center of circle of all reacting holes (12) is circumferentially same, and the center of circle of all reaction zones (22) is also circumferentially same.
4. the papery microfluid for isothermal duplication nucleic acid according to claim 1, is characterized in that: described hydrophobic material is paraffin or dimethyl siloxane or SU-8 photoresistance glue.
5. the papery microfluid for isothermal duplication nucleic acid according to any one of Claims 1 to 4, it is characterized in that: also comprise the zone of heating (3) being positioned at responding layer (2) below, described zone of heating (3) is made up of filter paper, and its lower surface is provided with resistance wire (31).
6. the papery microfluid for isothermal duplication nucleic acid according to claim 5, is characterized in that: the resistance wire (31) of described zone of heating (3) is for be formed by metal powder spray precipitation.
7. the papery microfluid for isothermal duplication nucleic acid according to claim 5, it is characterized in that: it is fixing that described application of sample layer (1), responding layer (2) and zone of heating (3) paste superposition by layers of two-sided (4), the layers of two-sided (4) between wherein said application of sample layer (1), between application of sample layer (1) with responding layer (2) is provided with and the well (11) of the application of sample layer (1) of the side of being located thereon and hole corresponding to reacting hole (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420583698.XU CN204211746U (en) | 2014-10-09 | 2014-10-09 | For the papery microfluid of isothermal duplication nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420583698.XU CN204211746U (en) | 2014-10-09 | 2014-10-09 | For the papery microfluid of isothermal duplication nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204211746U true CN204211746U (en) | 2015-03-18 |
Family
ID=52980433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420583698.XU Expired - Fee Related CN204211746U (en) | 2014-10-09 | 2014-10-09 | For the papery microfluid of isothermal duplication nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204211746U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400693A (en) * | 2015-12-15 | 2016-03-16 | 上海海洋大学 | Flat plate isothermal nucleic acid amplification chip |
| CN105400692A (en) * | 2015-12-15 | 2016-03-16 | 上海海洋大学 | Isothermal nucleic acid amplification device and isothermal nucleic acid amplification experimental method |
| CN107988046A (en) * | 2018-01-23 | 2018-05-04 | 吉林大学 | Self-absorption multichannel detection of pathogens micro-fluidic chip based on LAMP |
| CN108693347A (en) * | 2018-05-14 | 2018-10-23 | 广州万孚生物技术股份有限公司 | Multichannel immunochromatography label pad and production method and detection reagent card |
-
2014
- 2014-10-09 CN CN201420583698.XU patent/CN204211746U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400693A (en) * | 2015-12-15 | 2016-03-16 | 上海海洋大学 | Flat plate isothermal nucleic acid amplification chip |
| CN105400692A (en) * | 2015-12-15 | 2016-03-16 | 上海海洋大学 | Isothermal nucleic acid amplification device and isothermal nucleic acid amplification experimental method |
| CN105400692B (en) * | 2015-12-15 | 2017-06-27 | 上海海洋大学 | Isothermal nucleic acid amplification device and isothermal nucleic acid amplification experimental technique |
| CN107988046A (en) * | 2018-01-23 | 2018-05-04 | 吉林大学 | Self-absorption multichannel detection of pathogens micro-fluidic chip based on LAMP |
| CN108693347A (en) * | 2018-05-14 | 2018-10-23 | 广州万孚生物技术股份有限公司 | Multichannel immunochromatography label pad and production method and detection reagent card |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204211746U (en) | For the papery microfluid of isothermal duplication nucleic acid | |
| CN104293945B (en) | Method for carrying out nucleic acid isothermal amplification by using paper-based microfluid | |
| CN206334683U (en) | A kind of CD plate-likes micro-fluidic chip | |
| CN102886280B (en) | Microfluidic chip and application thereof | |
| WO2020177773A1 (en) | Multi-channel microfluidic blood coagulation measurement chip having five-layer structure | |
| Ge et al. | Photoelectrochemical lab-on-paper device based on an integrated paper supercapacitor and internal light source | |
| CN104122393B (en) | A kind of preparation of Photoelectrochemicalthree three-dimensional paper chip and the application in lesion detection thereof | |
| US10073091B2 (en) | Lateral flow assay device | |
| CN101571538B (en) | Patterned biochemical analysis test paper and manufacturing method and application thereof | |
| US20030124623A1 (en) | Microfluidic device and surface decoration process for solid phase affinity binding assays | |
| CN105296349A (en) | Microfluidic chip, detection system and device used for rapid DNA detection | |
| WO2020177774A1 (en) | Multi-channel microfluidic blood coagulation measurement chip | |
| CN205170857U (en) | A micro -fluidic chip , detecting system and device for DNA short -term test | |
| CN104407036A (en) | Preparation and application of electrochemical micro-fluidic device for nucleic acid isothermal amplification | |
| CN103645229A (en) | Array type multi-electrochemical isothermal amplification chip for detecting bacteria and preparation method thereof | |
| CN102604824A (en) | Space-oriented mini micro-fluidic real-time fluorescent PCR (Plymerase Chain Reaction) working system | |
| CN202415561U (en) | Quantitative PCR (Polymerase Chain Reaction) microfluidic control chip device | |
| KR102639604B1 (en) | Passive pumps for microfluidic devices | |
| CN205844190U (en) | A kind of paper substrate micro fluidic device | |
| CN205152235U (en) | A micro -fluidic chip , detecting system and device for gene somatotype detects | |
| EP3341128B1 (en) | Device and method for analysing liquid samples | |
| KR101770557B1 (en) | Biomolecular preconcentrating device | |
| KR101789043B1 (en) | Origami-based biosample concentration device | |
| CN202730131U (en) | Space oriented miniature microfluidic real-time fluorescent photo-conductive relay (PCR) work system | |
| CN101063674B (en) | Micro-domain heating apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150318 Termination date: 20191009 |