CN204198741U - A kind of poly functional reagent box - Google Patents
A kind of poly functional reagent box Download PDFInfo
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- CN204198741U CN204198741U CN201420653901.6U CN201420653901U CN204198741U CN 204198741 U CN204198741 U CN 204198741U CN 201420653901 U CN201420653901 U CN 201420653901U CN 204198741 U CN204198741 U CN 204198741U
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Abstract
The utility model discloses a kind of poly functional reagent box.This test kit comprises lid, box body, liner, the container that the reagent of QPCR is housed, the container that the reagent of siRNA transfection is housed, the container that the reagent of lysis is housed or reagent bottle.The utility model structure is simple, reasonable in design, easy to use, not vulnerable to pollution.
Description
Technical field
The utility model belongs to biological technical field, relates to a kind of poly functional reagent box, the reagent containing cell transfecting, lysis, QPCR experiment in this test kit.
Background technology
HnRNPs endonuclearly has the ribonucleoprotein of process regulating precursor RNA to shear, splice, transcribe a kind of being mainly present in, and is extensively present in various histocyte.HnRNPA2/B1 is the Major Members of hnRNPs family, hnRNPA2/B1 can with strand Telomerase tumor-necrosis factor glycoproteins specific binding, play protection Telomerase and prevent nuclease from making it degrade, and activate the effect of Telomerase.
AXL is a kind of receptor tyrosine kinase receptor tyrosine kinases (RTKs), AXL and other two kinds of RTKs:Tyro3 and Mer is called TAM tyrosine kinase receptors subfamily jointly, research proves that the activation of TAM and signal transduction work in many biological behaviours of cell, comprises the aspects such as cell survival, propagation, migration, adhesion.There is research to be presented in kinds of tumors the high expression level that there is AXL, comprise acute leukemia, mammary cancer, colorectal carcinoma, lung cancer, ovarian cancer and prostate cancer.
RNA interference (siRNA) is sequence specific gene silencing phenomenon [Fire A., the et a1.Potent and specific genetic interference bydouble-stranded RNA in Caenorhabditis elegans.Nature 1998 of a kind of double chain RNA mediate extensively existed in body; 391:806-811], because of its have that silence efficiency is high, high specificity and easy and simple to handle etc. be better than the advantage of traditional gene knockout means and developed rapidly, ready-made is research tool [Arziman Z., et al.E-RNAi:a webapplication to design optimized RNAi constructs.Nucleic Acids Res2005 important in life science; 33:W582-W588].SiRNA and protein bound form RNA and induce silencing complex (RNA-induced silencing complex, RISC).The mRNA that this mixture can be attached to homology induce it to degrade.
Y.He in its research in 2009 by high flux gene chip examination technology for detection to the expression artificially being reduced hnRNPA2/B1 in Colo16 squamous cell carcinoma system by RNA perturbation technique, cause the differential expression of 123 target genes in downstream, wherein just comprise AXL [He Y, et al., Downstream targets ofheterogeneous nuclear ribonucleoprotein A2 mediate cell proliferation, Molecularcarcinogenesis, 2009, 48 (2): 167-179], there is interactional possibility both us in preliminary prompting.But the research more deep about the two dependency has no report at present.
In order to the relation of hnRNPA2/B1 gene in study of lung cancer cells and AXL gene, cancer cell proliferation and growth, applicant disturbs the expression of hnRNPA2/B1 gene in lung carcinoma cell, detects it on the impact of AXL genetic expression and detect its impact on cancer cell proliferation and growth.Need to carry out the experiments such as siRNA transfection, lysis, RNA extraction, QPCR in the process of research, therefore need to use a large amount of reagent, the dispensing reagents used in each experimental procedure is placed and is brought inconvenience to operator, mentioned reagent is found as needed a large amount of time, therefore mentioned reagent is placed by classification in many times that operator can be saved in same place, and conveniently carries out same experiment in the future.
Summary of the invention
In order to solve the deficiencies in the prior art, the purpose of this utility model is to provide a kind of poly functional reagent box.Reagent containing cell transfecting, lysis, QPCR experiment in described test kit, facilitates experimenter to test continuously, saves the time finding reagent.The utility model structure is simple, reasonable in design, easy to use, not vulnerable to pollution.
To achieve these goals, the utility model adopts following technical scheme: a kind of poly functional reagent box, described test kit comprises lid 1, box body 2, liner 3, baffle plate 4, the container 5 of 5 × PrimeScript RT Master Mix is housed, the container 6 of hnRNPA2/B1 gene QPCR upstream primer and the container 7 of downstream primer are housed, the container 8 of AXL gene QPCR upstream primer and the container 9 of downstream primer are housed, the container 10 of GAPDH gene QPCR upstream primer and the container 11 of downstream primer are housed, the container 12 of SYBR dyestuff is housed, container 13 for the siRNA of hnRNPA2/B1 gene is housed, the container 14 of negative control siRNA is housed, be equipped with
container 15, be equipped with PMSF container 16, the container 17 of RNAiso Plus is housed, the reagent bottle 18 of RIPA lysate is housed, described liner 3 is positioned in described box body 2, and described liner 3 is provided with container hole 31 and groove 32, 3 described liners 3 separate and are fixed on described box body 2 by 2 described baffle plates 4, liner 3 described in the leftmost side is only provided with described container hole 31, middle described liner 3 is only provided with described container hole 31, and rightmost side liner 3 is provided with described groove 32 and described container hole 31, the size of described container hole 31 and the size of each container match, the size of described groove 32 and the size of described reagent bottle 18 match, the reagent bottle 18 of the described RIPA of being equipped with lysate and the container 16 of the described PMSF of being equipped with and described in RNAiso Plus is housed container 17 be positioned on liner 3 described in the rightmost side, be equipped with for the siRNA of hnRNPA2/B1 gene container 13 and the container 14 of negative control siRNA is housed and is equipped with
container 15 be positioned on middle described liner 3, other containers are positioned on liner 3 described in the leftmost side.
Preferably, described container is the EP pipe of 1.5ml.
Preferably, described container hole 31 runs through liner 3.More preferably, described container hole 31 is upper end open, lower end closed.
Preferably, described container hole 31 is arranged in parallel on liner.
Advantage of the present utility model and beneficial effect:
(1) the utility model contains the reagent of cell transfecting, lysis, QPCR experiment, facilitates experimenter to take reagent, tests continuously, save time.
(2) the utility model structure is simple, reasonable in design, easy to use, not vulnerable to pollution.
Accompanying drawing explanation
Fig. 1 is structural map of the present utility model;
Wherein, 1. lid; 2. box body; 3. liner; 31. container holes; 32. grooves; 4. baffle plate; 5. the container of 5 × PrimeScriptRT Master Mix is housed; 6. the container of hnRNPA2/B1 gene QPCR upstream primer is housed; 7. the container of hnRNPA2/B1 gene QPCR downstream primer is housed; 8. the container of AXL gene QPCR upstream primer is housed; 9. the container of AXL gene QPCR downstream primer is housed; 10. the container of GAPDH gene QPCR upstream primer is housed; 11. containers that GAPDH gene QPCR downstream primer is housed; 12. containers that SYBR dyestuff is housed; 13. containers that the siRNA for hnRNPA2/B1 gene is housed; 14. containers that negative control siRNA is housed; 15. are equipped with
container; 16. containers that PMSF is housed; 17. containers that RNAiso Plus is housed; 18. reagent bottles that RIPA lysate is housed.
Embodiment
Be further described embodiment of the present utility model below in conjunction with embodiment, advantage and disadvantage of the present utility model will be more clear along with description.But these embodiments are only exemplary, do not form any restriction to scope of the present utility model.
Embodiment 1 poly functional reagent box
A kind of poly functional reagent box, this test kit comprise lid 1, box body 2, liner 3, baffle plate 4, the container 5 that 5 × PrimeScript RT Master Mix is housed, the container 6 that hnRNPA2/B1 gene QPCR upstream primer is housed and the container 9 of the container 7 of downstream primer, the container 8 that AXL gene QPCR upstream primer is housed and downstream primer, the container 10 that GAPDH gene QPCR upstream primer is housed and downstream primer container 11, be equipped with SYBR dyestuff container 12, be equipped with for the siRNA of hnRNPA2/B1 gene container 13, be equipped with negative control siRNA container 14, be equipped with
container 15, be equipped with PMSF container 16, the container 17 of RNAiso Plus is housed, the reagent bottle 18 of RIPA lysate is housed; Liner 3 is positioned in described box body 2, and liner 3 is provided with container hole 31 and groove 32; 3 liners 3 separate and are fixed on box body 2 by 2 baffle plates 4, and leftmost side liner 3 is only provided with container hole 31, interface 3 is only provided with container hole 31, rightmost side liner 3 is provided with groove 32 and container hole 31; The size of container hole 31 and the size of each container match; The size of groove 32 and the size of reagent bottle 18 match; The reagent bottle 18 that RIPA lysate is housed and the container 16 that PMSF is housed and the container 17 that RNAiso Plus is housed are positioned on rightmost side liner 3, be equipped with for the siRNA of hnRNPA2/B1 gene container 13 and the container 14 of negative control siRNA is housed and is equipped with
container 15 be positioned on interface 3, other containers are positioned on leftmost side liner 3.
Wherein, container is the EP pipe of 1.5ml; Container hole 31 runs through liner 3, and container hole 31 is upper end open, lower end closed; Container hole 31 is arranged in parallel on liner.
In sum, embodiment of the present utility model only provides best embodiment, technology contents of the present utility model and technical characterstic disclose as above, but the personage the being familiar with the technology content that still may disclose based on the utility model and do the various replacement and the modification that do not deviate from the utility model creation spirit; Therefore, protection domain of the present utility model is not limited to the technology contents that embodiment discloses, therefore all equivalence changes done according to shape of the present utility model, structure and principle, be all encompassed in protection domain of the present utility model.
Claims (4)
1. a poly functional reagent box, it is characterized in that, described test kit comprises lid (1), box body (2), liner (3), baffle plate (4), the container (5) of 5 × PrimeScript RT Master Mix is housed, the container (6) of hnRNPA2/B1 gene QPCR upstream primer and the container (7) of downstream primer are housed, the container (8) of AXL gene QPCR upstream primer and the container (9) of downstream primer are housed, the container (10) of GAPDH gene QPCR upstream primer and the container (11) of downstream primer are housed, the container (12) of SYBR dyestuff is housed, the container (13) of the siRNA for hnRNPA2/B1 gene is housed, the container (14) of negative control siRNA is housed, be equipped with
the container (15) of 2000, be equipped with PMSF container (16), the container (17) of RNAiso Plus is housed, the reagent bottle (18) of RIPA lysate is housed, described liner (3) is positioned in described box body (2), and described liner (3) is provided with container hole (31) and groove (32), 3 described liners (3) separate and are fixed on described box body (2) by 2 described baffle plates (4), liner described in the leftmost side (3) is only provided with described container hole (31), middle described liner (3) is only provided with described container hole (31), and liner described in the rightmost side (3) is provided with described groove (32) and described container hole (31), the size of described container hole (31) and the size of each container match, the size of described groove (32) and the size of described reagent bottle (18) match, the reagent bottle (18) of the described RIPA of being equipped with lysate and the container (16) of the described PMSF of being equipped with and described in RNAiso Plus is housed container (18) be positioned on liner described in the rightmost side (3), described in be equipped with the siRNA for hnRNPA2/B1 gene container (13) and described negative control siRNA is housed container (14) and described in be equipped with
the container (15) of 2000 is positioned on middle described liner (3), and other containers are positioned on liner described in the leftmost side (3).
2. poly functional reagent box according to claim 1, is characterized in that, described container hole (31) runs through described liner (3).
3. poly functional reagent box according to claim 1, is characterized in that, described container hole (31) upper end open, lower end closed.
4. poly functional reagent box according to claim 1, is characterized in that, described container hole (31) is parallel on described liner (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420653901.6U CN204198741U (en) | 2014-11-04 | 2014-11-04 | A kind of poly functional reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420653901.6U CN204198741U (en) | 2014-11-04 | 2014-11-04 | A kind of poly functional reagent box |
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| Publication Number | Publication Date |
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| CN204198741U true CN204198741U (en) | 2015-03-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420653901.6U Expired - Fee Related CN204198741U (en) | 2014-11-04 | 2014-11-04 | A kind of poly functional reagent box |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388428A (en) * | 2014-10-22 | 2015-03-04 | 中国医科大学附属第一医院 | Double-chain siRNA for interfering hnRNPA2/B1 gene expression and application thereof |
| CN107096579A (en) * | 2017-05-10 | 2017-08-29 | 贵州大学 | A kind of multi-function animal tissue sampling holder |
-
2014
- 2014-11-04 CN CN201420653901.6U patent/CN204198741U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388428A (en) * | 2014-10-22 | 2015-03-04 | 中国医科大学附属第一医院 | Double-chain siRNA for interfering hnRNPA2/B1 gene expression and application thereof |
| CN104388428B (en) * | 2014-10-22 | 2016-09-14 | 中国医科大学附属第一医院 | A kind of double-strand siRNA disturbing hnRNPA2/B1 gene expression and application thereof |
| CN107096579A (en) * | 2017-05-10 | 2017-08-29 | 贵州大学 | A kind of multi-function animal tissue sampling holder |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150311 Termination date: 20171104 |
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| CF01 | Termination of patent right due to non-payment of annual fee |