CN204129053U - The pick-up unit rotated around axle center - Google Patents

The pick-up unit rotated around axle center Download PDF

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Publication number
CN204129053U
CN204129053U CN201420675699.7U CN201420675699U CN204129053U CN 204129053 U CN204129053 U CN 204129053U CN 201420675699 U CN201420675699 U CN 201420675699U CN 204129053 U CN204129053 U CN 204129053U
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CN
China
Prior art keywords
chamber
reagent
pick
up unit
fluid channel
Prior art date
Application number
CN201420675699.7U
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Chinese (zh)
Inventor
胡贵权
Original Assignee
杭州欣安医疗科技有限公司
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Priority to CN201420675699.7U priority Critical patent/CN204129053U/en
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Publication of CN204129053U publication Critical patent/CN204129053U/en

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Abstract

The utility model discloses a kind of pick-up unit based on rotating around axle center.Pick-up unit comprises: upper strata, comprises liquid filling hole; Middle layer, comprises at least one spout hole and at least one fluid channel, and wherein spout hole is used for quantitatively, and fluid channel is used for reagent is passed through under the action of the centrifugal; And lower floor, its at the middle and upper levels, middle layer with lower floor watertightness in conjunction with forming reactions chamber, wherein reaction chamber away from the sidewall in axle center and vertical direction at an angle.This pick-up unit have dirigibility high, realize quantitatively detecting, have batch concurrently and the advantage such as to detect individually.

Description

The pick-up unit rotated around axle center

Technical field

The utility model relates to detection field, is specifically related to the pick-up unit based on centrifugal rotation technology.

Background technology

In medical test, environmental analysis, food and the field such as drug inspection and safety detection, large-scale experiment room analytical instrument automaticity is high, detection is accurate, is for a long time to analyze the core tool detected.But the application of these laboratory analytical instrument still because before analyzing, analyze after step at substantial time of many complexity, survey report can not be obtained in the short time, in times of crisis can not in time for race against time in location and dealing with problems.

Current every profession and trade proposes more and more urgent requirement to Site Detection and real-time thereof, along with the development of biotechnology, immunological technique, micro-electromechanical technology and sensing technology, has occurred portable small-sized express-analysis instrument.Due to easy and simple to handle, detect in time, the portable small-sized express-analysis instrument occasion that requirement of real-time is high at the scene plays more and more important effect, just developing into analyze detect in the branch new with arranged side by side one of laboratory analytical instrument.

Instrumental method and chemical method can be divided in portable analysers.Dry chemical method and wet chemistry method can be divided in chemical method.Dry chemical method feature is that reagent is present in chip with dry powder or freeze-dried powder state, and wet chemistry method feature is that reagent exists with solution state.The reagent of dry chemical method is nuzzled in chip in advance, and the application of this chip is just fixed, and loses dirigibility, and pre-buried reagent limited by the quality guarantee time limit.

Utility model content

In order to solve the problem, the utility model proposes the pick-up unit rotated around axle center.

Based on the pick-up unit rotated around axle center, comprising:

Upper strata, is provided with liquid filling hole;

Middle layer, comprises at least one spout hole and at least one fluid channel, and wherein spout hole is used for quantitatively, and fluid channel is used for reagent is passed through under the action of the centrifugal; And

Lower floor, its at the middle and upper levels, middle layer with lower floor watertightness in conjunction with forming reactions chamber, for carrying out physics or chemical reaction, wherein reaction chamber is acute angle away from the sidewall in axle center and vertical direction.

Described middle layer comprises reactive tank, and reactive tank is combined the reaction chamber described in being formed with the upper and lower, the chamber wall of wherein reactive tank has described spout hole and fluid channel, and this fluid channel is positioned at the chamber wall of the reactive tank away from axle center.

The upper surface of described lower floor is provided with growth substrate, and middle layer is provided with press mold ring, and press mold ring is used for fixed growth substrate.

Described upper strata comprises pilot hole further, for pick-up unit is fixed on centrifugal device.

Described middle layer comprises reagent trough further, the chamber wall of reagent trough has spout hole, reagent trough to be combined with middle layer watertightness by upper strata and to form reagent chamber, reagent enters reagent chamber from liquid filling hole, reagent enters reaction chamber from reagent chamber by fluid channel under the action of the centrifugal, and after reaction, reagent is discharged from reaction chamber by another fluid channel.

Described liquid filling hole comprises the first liquid filling hole and the second liquid filling hole, and the first liquid filling hole aims at reagent chamber, and the second liquid filling hole aims at reaction chamber.

Described fluid channel is arc rotating shape.

Described upper strata comprises multiple liquid filling hole;

Wall comprises the first reagent trough, the first spout hole and the first fluid channel, and wherein the first spout hole is positioned at the sidewall of the first reagent trough;

Middle layer comprises the second reagent trough, reactive tank, the second spout hole, the second fluid channel and waste liquid runner, and the second spout hole is positioned at the sidewall of the second reagent trough;

Upper strata, wall and middle layer are carried out watertightness combination and are formed the first reagent chamber, the second reagent chamber and reaction chamber respectively, reaction chamber from axle center farthest, wherein the first fluid channel UNICOM first reagent chamber and reaction chamber, second fluid channel UNICOM second reagent chamber and reaction chamber, waste liquid runner is used for waste liquid in reaction chamber to discharge.

A kind of pick-up unit for rotating around axle center, comprise the first chamber, the second chamber and waste liquid tank, wherein the first chamber and the second chamber present acute angle away from the sidewall in axle center and vertical direction, second chamber is positioned at axle center far-end, first chamber sidewall has spout hole for quantitatively, by fluid channel UNICOM between first chamber and the second chamber, the second chamber and waste liquid tank are by waste liquid runner UNICOM.

The pick-up unit rotated around axle center that the utility model proposes and detection method, integrate process, dilution, multistep reaction, have dirigibility high, realize quantitatively detecting, have batch concurrently and the advantage such as to detect individually.

Accompanying drawing explanation

Fig. 1 shows according to a kind of liquid Controlling model schematic diagram of the present utility model;

Fig. 2 shows the schematic cross-section of the pick-up unit 100 rotated around axle center according to the utility model embodiment 1;

Fig. 3 shows the vertical view according to pick-up unit in Fig. 2 100;

Fig. 4 shows the schematic cross-section of the pick-up unit 100 according to the utility model embodiment 2;

Fig. 5 shows the vertical view according to pick-up unit in Fig. 4 100;

Fig. 6 shows the vertical view in the middle layer 12 according to the utility model embodiment 2;

Fig. 7 shows the vertical view of the pick-up unit 100 according to the utility model embodiment 2;

Fig. 8 shows the sectional view of the pick-up unit 100 according to the utility model embodiment 3; And

Fig. 9 shows the detection method schematic flow sheet according to the utility model embodiment 4.

Embodiment

Specific embodiment of the utility model will be described in detail below.It should be noted that the embodiments described herein is only for illustrating, is not limited to the utility model.

Fig. 1 shows a kind of liquid Controlling model schematic diagram according to the utility model one embodiment.Illustrated circular conical surface becomes α angular movement with surface level, is that ω circles with angular velocity.Be the particle of m in the quality of distance center axle r position, it is stressed two, and one is the anchorage force in vertical conical surface direction, and two is gravity, when this particle can maintain vertical direction without relative motion, sets up equation as follows:

Vertical direction: G=Ncos α (1)

Horizontal direction: Fr=Nsin α=m ω 2r (2)

Can obtain according to equation (1) and (2):

ω 2=g×tanα÷r???????????????????????????????(3)

Wherein, g is acceleration of gravity, constant;

R is circular motion radius;

Tan α, the conical surface and horizontal plane angle tangent value are constant in fixed angle situation.

At circular motion r place, known according to formula (2), when angular velocity be greater than circular motion radius and tangent value, acceleration of gravity long-pending time, particle m can not maintain the balance of vertical direction, will along tilted cone face upper direction.If when establishing the passage of direction as shown above circular conical surface, along with the increase of acceleration, owing to not having inclined-plane to continue to provide support power at this passage place, fluids along channels flows out.According to the effect that this centrifugal motion produces, can control by convection cell, fluid is flowed by fluid channel between different chamber.And then be applied in micro-pick-up unit in the control of reagent.

embodiment 1

Fig. 2 shows the schematic cross-section of the pick-up unit 100 rotated around axle center according to the utility model one embodiment.Pick-up unit 100 comprises upper strata 11, middle layer 12 and lower floor 13.For the ease of distinguish, Fig. 2 is superincumbent partially illustrate assembling before pick-up unit 100.The pick-up unit 100 partially illustrated after assembling below.In at least certain stage of application, pick-up unit 100 rotates around axle center Z under force.It 11 comprises liquid filling hole at the middle and upper levels, by this hole by reactant liquor, detect the liquid such as liquid, washing agent and add.Middle layer 12 comprises reactive tank 121, is the space that middle layer 12 surrounds, wherein reactive tank 121 away from the chamber wall of axle center Z and vertical direction at an angle.The chamber wall in middle layer 12 also comprises and penetrates the spout hole 122 in middle layer 12 and the waste liquid runner 123 of effluent discharge.In one embodiment, waste liquid runner is a kind of fluid channel, chamber, the outside wall of reactive tank is circular conical surface, and reagent under centrifugal rotation effect is outwards discharged by waste liquid runner 123, and wherein centrifugal rotation speed regulates according to parameters such as the radiuses of reagent viscosity, reactive tank.Lower floor 13 is a substrate.Upper strata 11, middle layer 12 and lower floor 13 are carried out watertightness combination, forms the pick-up unit 100 after assembling.Watertightness combination comprise adopt that glue carries out that watertightness is bonding, one or more among ultra-sonic welded, laser bonding, microwave joining.Can see from the pick-up unit after assembling, the reactive tank 121 of pick-up unit 100 and lower floor 13 combine and define independently reaction chamber 124.In one embodiment, the upper surface of lower floor 13 can comprise growth substrate 126.Growth substrate 126 can be the diaphragm of special substance.Growth substrate can adopt polystyrene, polycarbonate, poly-cellulose nitrate, silicon, glass and metal species make.Profile can be rectangle, ellipse, rectangle, fan-shaped, triangle and combination in the shape.Middle layer 12 can comprise press mold ring 125, is fitted in watertightness in reaction chamber 124, for fixing growth substrate 126, and makes growth substrate 126 be emerging in the bottom surface of reaction chamber 124.Reagent A adds reaction chamber 124 from liquid filling hole 111, and by spout hole 122 overflow, makes reagent A have fixing volume.In an Application Example, first the first reagent is added reaction chamber 124 by liquid filling hole 111, make the active substance in reagent be fixed in growth substrate 126 as reaction reagent, then reacted reagent is discharged by waste liquid runner 123 by rotating centrifugal; Add the second reagent again, the second reagent and active substance are reacted.Active substance can be antibody, antigen and enzyme, is fixed in growth substrate 126 by modes such as absorption or bondings.Described growth substrate 126 can directly be adsorbed or bonding reagent through chemistry, physical treatment.

Fig. 3 shows the vertical view of the pick-up unit 100 according to the utility model one embodiment.This top view show the upper strata of this pick-up unit 100.From this vertical view, the upper strata 100 of pick-up unit 100 comprises liquid filling hole 111.In one embodiment, pick-up unit 100 comprises fixed orifice 113 further, and for pick-up unit 100 is fixed to centrifugal device, under dynamic action, pick-up unit 100 does centrifugal motion with centrifugal device.Pick-up unit 100 comprises multiple liquid filling hole 111, wherein the corresponding reaction chamber of each liquid filling hole.Therefore, pick-up unit 100 can detect multiple sample simultaneously.

embodiment 2

Fig. 4 shows the schematic cross-section of the pick-up unit 100 according to another embodiment of the utility model.Pick-up unit 100 comprises upper strata 11, middle layer 12 and lower floor 13.Upper strata 11 comprises the first liquid filling hole 111 and the second liquid filling hole 112.Wherein the second liquid filling hole 112 to the first liquid filling hole 111 is near inner side, namely near turning axle Z.Middle layer comprises the reagent trough 221 being positioned at inner side and the hollow reactive tank 121 formed by middle layer 12 being positioned at outside.Wherein the outside inwall of reagent trough 221 and reactive tank 121 is inclined-plane, with vertical direction at an angle.The madial wall of reagent trough 221 or reactive tank 121 can be any feasible shape, as taper shape, trapezoidal inclined plane or tetrahedral structure etc.Reagent trough 221 comprises spout hole 122 by wall top, paracentral chamber.The upper part of middle layer 12 between reagent trough 221 and reactive tank 121 comprises fluid channel 224.Middle layer 12 comprises waste liquid runner 123 in the outer upper end of reactive tank 121.Middle layer can comprise press mold ring 125, is close to reactive tank inwall.Lower floor 13 upper surface can place growth substrate 126.After upper strata 11, middle layer 12 and lower floor 13 are carried out watertightness combination, form reaction chamber 124 and the reagent chamber 228 with closed space, under the action of the centrifugal, reagent flows to reaction chamber 124 from reagent chamber 228 by fluid channel 224.By press mold ring 125, growth substrate 126 is fixed simultaneously.In an Application Example, first reagent A is added reaction chamber 124 by the first liquid filling hole 111, make the active substance in reagent A be fixed in growth substrate 126; Carry out centrifugally being discharged by waste liquid runner 123 to remaining reagent A; Reagent B is added reagent chamber 228 by the second liquid filling hole 112, through spout hole 122 overflow, the reagent B of fixed volume is entered reaction chamber 124 by fluid channel 224 under the action of the centrifugal.Active substance in the reagent B of fixed volume and growth substrate 126 is reacted.Therefore accurate quantitative reaction can be realized.Again reacted reagent quantitatively detected or growth substrate 126 is detected, obtaining the detected value of detection material.

In one embodiment, pick-up unit 100 can comprise waste liquid tank further, connects waste liquid runner 123, for storing waste liquid.In one embodiment, pick-up unit comprises programmable chip further, and propulsion system couple, and for controlling liquid addition steps, rotating speed coupling, or coordinates sensitive element to realize Aulomatizeted Detect flow process further.In one embodiment, pick-up unit comprises propulsion system for providing centrifugal force, and sensitive element is for the reagent after detection reaction, and detection approach comprises photoresistance, imageing sensor, photoelectric cell, photomultiplier, photon counter etc.

Fig. 5 shows the vertical view of the pick-up unit 100 according to the utility model one embodiment.Pick-up unit 100 upper strata comprises the first liquid filling hole 111 and the second liquid filling hole 112.Second liquid filling hole 112 aims at reagent chamber 228, and the first liquid filling hole 111 aims at reaction chamber 124.Pick-up unit 100 also comprises pilot hole 113 further, for fixing with powerdriven centrifugal device.

Fig. 6 shows the vertical view in the middle layer 12 according to the utility model one embodiment.Middle layer 12 comprises reagent trough 221, reactive tank 121, and the fluid channel 224 be positioned on middle layer 12 and waste liquid runner 123.Wherein fluid channel 224 and waste liquid runner 123 are in arc rotating shape opposite to the direction of rotation, are beneficial to the discharge of reagent.

Fig. 7 shows the vertical view of the pick-up unit 100 according to the utility model one embodiment.Wherein pick-up unit 100 comprises multiple first liquid filling hole 111 and second liquid filling hole 112.The reagent added in the first liquid filling hole 111 flows into all reagent troughs adaptively by fluid channel, and obtains many parts of quantitative reagent by spout hole overflow.The method is applicable to comparison.

embodiment 3

Fig. 8 shows the sectional view of the pick-up unit 100 according to the utility model one embodiment.Pick-up unit 100 comprises upper strata 11, wall 14 and middle layer 12(middle layer in fact here as bottom).Wherein upper layer packets is containing multiple liquid filling hole 111,112 and 114.Wall 14 comprises the first reagent trough 321, first spout hole 322 and the first fluid channel 323, and wherein the first spout hole is positioned at the sidewall of the first reagent trough.Middle layer 12(comprises the second reagent trough 221, reactive tank 222, second spout hole 122, second fluid channel 224 and waste liquid runner 123, and wherein the second spout hole is positioned at the sidewall of the second reagent 221.By watertightness combination is carried out in upper strata, wall and middle layer, form the first reagent chamber 34, (second) reagent chamber 228 and reaction chamber 124 respectively, wherein the first fluid channel 323 UNICOM first reagent chamber 34 and reaction chamber 124, second fluid channel UNICOM reagent chamber 228 and reaction chamber 124, waste liquid in reaction chamber 124 is discharged by waste liquid runner 123.In an Application Example, first the first reagent is entered reagent trough 221 by liquid filling hole 112, or liquid filling hole 114 enters reagent trough 321, obtain quantitative reagent through overflow; Through centrifugal, reagent is entered reaction chamber 124 by fluid channel 323 or 224 again, active substance is fixed in growth substrate 126, and reacted waste liquid is discharged; In the first reagent trough 321 and the second reagent trough 221, add reagent A and reagent B more simultaneously, through centrifugal, quantitative reagent A or reagent B are entered reaction chamber 124 by fluid channel, the active substance in quantitative reagent A, reagent B and growth substrate 126 is reacted.In one embodiment, after emptying waste liquid, by upper strata liquid filling hole, add cleansing solution, will the waste liquid washing of reagent chamber, reaction chamber inwall be remained in, after appropriate time, under setting centrifugal action, scrub raffinate is entered waste liquid tank via fluid channel.

embodiment 4

Fig. 9 shows the detection method schematic flow sheet according to the utility model embodiment 2,3.The method is included in step 901 and first reagent is added reaction chamber; In step 902, the active substance in first reagent is adsorbed in growth substrate in the reactor chamber; By centrifugal, remaining waste liquid is discharged outside reaction chamber by waste liquid runner or fluid channel in step 903; In step 904, second reagent is added reagent chamber, and carry out overflow by the spout hole of reagent trough sidewall, realize quantitatively; By centrifugal, the second quantitative reagent is entered reaction chamber by another fluid channel in step 905, react with the active substance in reaction chamber.

In another embodiment, can simultaneously respectively to adding the second reagent and the 3rd reagent in the first reagent chamber and the second reagent chamber, second reagent chamber is also quantitative by overflow, and this conveniently comprises and by centrifugal motion, the second reagent and the 3rd reagent is entered reaction chamber simultaneously simultaneously, react with the active substance in reaction chamber.

According to the pick-up unit of the multiple embodiment of the utility model, be used in on-the site analysis human body or animal's whole blood, blood plasma, serum, saliva, urine, sweat, seminal fluid and other liquid in medical test.The water qualities such as sewage, surface water, potable water can be analyzed by spot sampling in the environment.In field of food safety, section's spot sampling analyzes residues of pesticides, bacterium, virus etc.Be particularly suitable for complex sample solution to detect, out analyze fixedly separated for tested substance, especially in the situation based on specific bond such as enzyme, immunoreagent, the material needed is combined fixing, loose material enters waste liquid tank by fluid channel discharge opeing under centrifugal action, thus realize being separated, decrease the interference that other material is possible.

Above disclosure only relates to preferred embodiment or embodiment, many modifications can be produced and do not depart from claims propose spirit and scope of the present utility model, it should not be construed as the restriction to the utility model protection domain.Specific embodiment described by this instructions is only for illustration of object, and those skilled in the art, in spirit of the present utility model and principle, can draw multiple amendment or equivalent.The protection domain that the utility model is contained is as the criterion with appended claims.Therefore fall into change in claim or its equivalent scope and remodeling to be claim of enclosing and to contain.

Claims (9)

1. based on the pick-up unit rotated around axle center, it is characterized in that, comprising:
Upper strata, is provided with liquid filling hole;
Middle layer, comprises at least one spout hole and at least one fluid channel, and wherein spout hole is used for quantitatively, and fluid channel is used for reagent is passed through under the action of the centrifugal; And
Lower floor, its at the middle and upper levels, middle layer with lower floor watertightness in conjunction with forming reactions chamber, for carrying out physics or chemical reaction, wherein reaction chamber is acute angle away from the sidewall in axle center and vertical direction.
2. pick-up unit as claimed in claim 1, it is characterized in that, described middle layer comprises reactive tank, reactive tank is combined the reaction chamber described in being formed with the upper and lower, the chamber wall of wherein reactive tank has described spout hole and fluid channel, and this fluid channel is positioned at the chamber wall of the reactive tank away from axle center.
3. pick-up unit as claimed in claim 2, it is characterized in that, the upper surface of described lower floor is provided with growth substrate, and middle layer is provided with press mold ring, and press mold ring is used for fixed growth substrate.
4. pick-up unit as claimed in claim 1, it is characterized in that, described upper strata comprises pilot hole further, for pick-up unit is fixed on centrifugal device.
5. pick-up unit as claimed in claim 1, it is characterized in that, described middle layer comprises reagent trough further, the chamber wall of reagent trough has spout hole, reagent trough to be combined with middle layer watertightness by upper strata and to form reagent chamber, reagent enters reagent chamber from liquid filling hole, and reagent enters reaction chamber from reagent chamber by fluid channel under the action of the centrifugal, and after reaction, reagent is discharged from reaction chamber by another fluid channel.
6. pick-up unit as claimed in claim 5, it is characterized in that, described liquid filling hole comprises the first liquid filling hole and the second liquid filling hole, and the first liquid filling hole aims at reagent chamber, and the second liquid filling hole aims at reaction chamber.
7. pick-up unit as claimed in claim 1, it is characterized in that, described fluid channel is arc rotating shape.
8. pick-up unit as claimed in claim 1, is characterized in that,
Upper strata comprises multiple liquid filling hole;
Wall comprises the first reagent trough, the first spout hole and the first fluid channel, and wherein the first spout hole is positioned at the sidewall of the first reagent trough;
Middle layer comprises the second reagent trough, reactive tank, the second spout hole, the second fluid channel and waste liquid runner, and the second spout hole is positioned at the sidewall of the second reagent trough;
Upper strata, wall and middle layer are carried out watertightness combination and are formed the first reagent chamber, the second reagent chamber and reaction chamber respectively, reaction chamber from axle center farthest, wherein the first fluid channel UNICOM first reagent chamber and reaction chamber, second fluid channel UNICOM second reagent chamber and reaction chamber, waste liquid runner is used for waste liquid in reaction chamber to discharge.
9. the pick-up unit for rotating around axle center, it is characterized in that, comprise the first chamber, the second chamber and waste liquid tank, wherein the first chamber and the second chamber present acute angle away from the sidewall in axle center and vertical direction, second chamber is positioned at axle center far-end, first chamber sidewall has spout hole for quantitatively, and by fluid channel UNICOM between the first chamber and the second chamber, the second chamber and waste liquid tank are by waste liquid runner UNICOM.
CN201420675699.7U 2014-11-13 2014-11-13 The pick-up unit rotated around axle center CN204129053U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420675699.7U CN204129053U (en) 2014-11-13 2014-11-13 The pick-up unit rotated around axle center

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420675699.7U CN204129053U (en) 2014-11-13 2014-11-13 The pick-up unit rotated around axle center

Publications (1)

Publication Number Publication Date
CN204129053U true CN204129053U (en) 2015-01-28

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CN201420675699.7U CN204129053U (en) 2014-11-13 2014-11-13 The pick-up unit rotated around axle center

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104483496A (en) * 2014-11-13 2015-04-01 杭州欣安医疗科技有限公司 Detection device rotating around axis and detection method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104483496A (en) * 2014-11-13 2015-04-01 杭州欣安医疗科技有限公司 Detection device rotating around axis and detection method thereof
CN104483496B (en) * 2014-11-13 2017-08-25 广东泓睿科技有限公司 The detection means and its detection method rotated around axle center

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150128

Termination date: 20161113

CF01 Termination of patent right due to non-payment of annual fee