CN204101449U - Based on the Streptavidin concentration sensor of Fiber Bragg Grating FBG - Google Patents
Based on the Streptavidin concentration sensor of Fiber Bragg Grating FBG Download PDFInfo
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- CN204101449U CN204101449U CN201420568330.6U CN201420568330U CN204101449U CN 204101449 U CN204101449 U CN 204101449U CN 201420568330 U CN201420568330 U CN 201420568330U CN 204101449 U CN204101449 U CN 204101449U
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Abstract
The utility model relates to a kind of Streptavidin concentration sensor based on Fiber Bragg Grating FBG, comprise Fibre Optical Sensor and solution to be measured, it is characterized in that, described sensor is by light supply apparatus, spectroanalysis instrument, Transmission Fibers, three ports light rings, optical fiber stationary platform, Fiber Bragg Grating FBG and solution composition to be measured, the light signal output end of described light supply apparatus is connected by first port of Transmission Fibers with described three ports light rings, second port of described three port circulators is connected with described Fiber Bragg Grating FBG by Transmission Fibers, described Fiber Bragg Grating FBG is fixed by described optical fiber stationary platform, 3rd port of described three port circulators is connected with described spectroanalysis instrument by Transmission Fibers.The utility model has that structure is simple, volume is little, measurement sensistivity is high, can carry out long-distance remote control monitoring and other advantages.
Description
Technical field
The utility model belongs to the crossing domain that optical fiber sensing technology is combined with bioprotein detection technique, be specifically related to a kind of Streptavidin concentration sensor based on Fiber Bragg Grating FBG, the sensitivity characteristic of the bragg wavelength utilizing the mechanism of biotin and Streptavidin specific binding and corrosivity Fiber Bragg Grating FBG ambient refractive index to external world, realizes the Concentration Testing of Streptavidin.
Background technology
Sensor is a kind of physical unit or biologic-organ, extraneous physical signalling such as power, sound, electricity, heat, light can be detected, also can experience the chemical composition of external environment such as temperature, humidity or smog, and the information found out is passed to other devices or organ.Sensor is generally by sensing unit, and converting unit and transmission unit form.The biologically active unit such as enzyme, antigen-antibody, nucleic acid are utilized to be referred to as biology sensor as the sensor of sensing unit.Conventional biosensor arrangement is complicated, and sample requirements is large, and with poisonous (fluorescence) indicator, chemical analysis very complicated, expends time in also long.
The ultimate principle of Fibre Optical Sensor is that the light from light source is sent into modulator through optical fiber, after parameter to be measured and the light entering modulator zone are interacted, the optical characteristics of light (intensity, wavelength, frequency, phase place, polarization state etc. as light) is caused to change, become by the flashlight be adjusted to, photo-detector is sent into again through optical fiber, after demodulation, obtain measured parameter.Because optical fiber structure is stablized, electrical insulating property is good, and anti-electromagnetic interference capability is strong, and high sensitivity, easily realizes the remote monitor of measured signal, is convenient to be connected with computing machine, is therefore widely used in sensory field.
Optical fiber biosensor is primarily of bio-sensing unit and optical fiber composition, using biologically active unit (enzyme, microorganism, animal vegetable tissue section, antigen-antibody, nucleic acid) as bio-sensing unit, utilize optical fiber as the reaction of signal converter captured target thing and responsive primitive, the extent of reaction is expressed by electric signal or light signal, thus obtains measured object information.This sensor can detect the change of biological respinse optical signal, by detecting the amount of the parameter determination analyte such as intensity, amplitude, phase place of light.Compared with other sensor, this sensor has by the interference of electromagnetic field, highly sensitive, quality is light, volume is little, be easy to multiplexing (networking), can the long-distance remote control advantage of monitoring.The application of optical fiber biosensor in life science, bioengineering, food industry, environment and agricultural etc., just at development, is especially applicable to remote measurement and on-line monitoring in biosome.
Streptavidin (Streptavidin, SA) is a kind of a kind of protein of purifying out from streptomycete, and molecular weight is 52.8k dalton.Because the chemical property that it is more favourable, so it can be used as the substitute of avidin.Streptavidin is a kind of tetramer, and be made up of four identical peptide chains, dissociation constant is 10
-14the mol/L order of magnitude, it and biotin (Biotin, also known as VB7 or biotin) have very strong suction-operated, and its every bar peptide chain can in conjunction with a biotin.The suction-operated of biotin and Streptavidin is one of current the strongest known noncovalent interaction, and both suction-operateds are species specific selective adsorptions, namely only have Avidin can only with Streptavidin generation specific adsorption, specific adsorption is there is not with other protein, there is selectivity, have very large potential in cancer cell, field of virus detection.In addition, Streptavidin-biotin system can effectively prevent organic solvent, denaturant (such as guanidine hydrochloride), washing agent and proteolytic enzyme from damaging it, also can retention properties stablize at extreme temperature and pH value, make Streptavidin be widely used in biomolecular science and bio-nanotechnology, the concentration measuring streptavidin is accurately vital.
Utilize certain density hydrofluoric acid solution, by the corrosion of the grid region of Fiber Bragg Grating FBG to certain degree of depth, the diameter of covering is made to be reduced to certain value, and surface silanization modification is carried out to the surface after corrosion, recycle covalently bound mechanism, make surface carry biotin, surface, grid region after corrosion forms specific adsorption rete, thus mutually can adsorb to be formed to Streptavidin and stablize conjugate.The refractive index on surface, grid region can be changed after the solution of streptavidin of variable concentrations is combined with biotin, thus affect the effective index of fundamental mode transmitted in fibre core, the bragg wavelength of grating being changed, by detecting the change of its spectrum, the measurement of solution of streptavidin concentration can be realized.
Summary of the invention
The purpose of this utility model is exactly: complicated in order to solve conventional biosensor device, sample requirements is large, with poisonous (fluorescence) indicator, chemical analysis very complicated, the shortcoming that detection time is long and precision is not high, provide that a kind of structure is simple, volume is little, can real time on-line monitoring be realized and precision high based on Fiber Bragg Grating FBG Streptavidin concentration sensor suddenly.
The technical scheme that the utility model is taked for technical solution problem is:
A kind of Streptavidin concentration sensor based on Fiber Bragg Grating FBG, it is characterized in that, described sensor is by light supply apparatus, spectroanalysis instrument, Transmission Fibers, three ports light rings, optical fiber stationary platform, Fiber Bragg Grating FBG and solution composition to be measured, the light signal output end of described light supply apparatus is connected by first port of Transmission Fibers with described three ports light rings, second port of described three port circulators is connected with described Fiber Bragg Grating FBG by Transmission Fibers, described Fiber Bragg Grating FBG is fixed by described optical fiber stationary platform, 3rd port of described three port circulators is connected with described spectroanalysis instrument by Transmission Fibers.
The grid region of described Fiber Bragg Grating FBG utilizes certain density hydrofluoric acid solution to corrode to certain depth, and the grid region finishing after corrosion has the rete of specific adsorption function.
Described optical fiber stationary platform upper surface should have a groove, and during fixed grating, grid region is in groove, and during to ensure that solution to be measured pours into groove, surface, grid region is immersed solution to be measured completely and contacted with uniform liquid.
The light that described light supply apparatus sends is from the first port input of three ports light rings, Fiber Bragg Grating FBG is outputted to from the second port, the grid region of Fiber Bragg Grating FBG utilizes certain density hydrofluoric acid solution to corrode to certain depth, the diameter of covering is made to be reduced to certain value, and surface film modification is carried out in the grid region after corrosion, forming the rete with specific adsorption function makes it produce specific adsorption effect to Streptavidin, grid region as sensitizing range is fixed in the groove of optical fiber stationary platform upper surface, when ensureing that solution to be measured pours into groove, surface, grid region is immersed solution to be measured completely and contacts with uniform liquid, reflected light signal after Fiber Bragg Grating FBG enters into spectroanalysis instrument via optical circulator from the 3rd port again, light signal is changed into concentration signal finally by process.
The beneficial effects of the utility model are:
1, as the Fiber Bragg Grating FBG that the grid region of bio-sensing unit is through corrosion, its manufacture craft comparative maturity, advantage of lower cost, and the effective index of fundamental mode transmitted in corrosion type Fiber Bragg Grating FBG fibre core to external world ambient refractive index change very sensitive, so sensitivity is very high; Fiber Bragg Grating FBG volume is little, and during detection, required sample size is few, solves the problem that conventional biosensor volume is large, sample requirements is large.
2, the technology of finishing carried out to optical fiber very ripe, the detection of other biomass can be applied to by the principle measuring Streptavidin concentration of analog bacterioprotein Concentration Testing, change the method for modifying on surface, corrosion type grating grid region, specificity adsorption selection can be produced to different materials, realize the identification of different material, as the Selective recognition of cancer cell proteins, greatly reduce testing cost and complicated testing process is become and more easily realize.
Accompanying drawing explanation
Fig. 1 is the system architecture schematic diagram of the utility model embodiment.
Embodiment
Below in conjunction with accompanying drawing, embodiment of the present utility model is elaborated.
As shown in Figure 1, for the system architecture schematic diagram of the utility model embodiment, the Streptavidin concentration sensor based on Fiber Bragg Grating FBG comprises light supply apparatus (10), spectroanalysis instrument (20), Transmission Fibers (30), three ports light rings (40), optical fiber stationary platform (50), Fiber Bragg Grating FBG (60) and solution to be measured (70).
The light signal that light supply apparatus (10) sends enters three ports light rings (40) by Transmission Fibers (30), and then arrive Fiber Bragg Grating FBG (60) through another root Transmission Fibers (30), described Fiber Bragg Grating FBG (60) detects solution to be measured (70), then optical signal feedback is carried out spectral analysis to spectroanalysis instrument (20).
Fiber Bragg Grating FBG (60) forms bio-sensing unit after processing as follows: first utilized in the grid region of Fiber Bragg Grating FBG certain density hydrofluoric acid solution to corrode to certain depth, the diameter of covering is made to be reduced to certain value, subsequently pre-service is carried out to the surface, grid region of corroding: with being immersed in the mixed solution of 30% hydrogen peroxide and 98% concentrated sulphuric acid after alcohol wipe, in solution, the volume ratio of two kinds of liquid is 1: 1, soak time is 30 minutes, makes surface, grid region form silicone hydroxyl; Then 3-aminopropyl three ethoxy silane (APTES is immersed in, 3-APS), in the solution that absolute ethyl alcohol and deionized water mix, the volume ratio of three kinds of liquid is 2.5: 96.5: 1,3-aminopropyl three ethoxy silane is a kind of silane coupling agent, the hydroxyl covalent bond that the alkyl of its one end can produce with surface, previous step grid region is adsorbed on surface, grid region, its other end then carries amino, the immersion time in grid region is 24 hours, surface, grid region is reacted fully with APTES, covalent bond carry out silane coupledization, and completes amido modified on surface, grid region; Biotin (NHS-Biotin) is finally used to modify surface, grid region, biotin the amino covalence of grid region finishing in previous step can be combined to be formed and stablizes conjugate, surface, grid region is made to carry biotin molecule like this, define specific adsorption rete, the biotin of rete can produce very strong suction-operated with the Streptavidin molecule in solution to be measured, thus completes the making of bio-sensing unit.
System operating mode of the present utility model is: the light signal that light supply apparatus (10) sends enters from three port circulator (40) first ports through Transmission Fibers (30), arrive the second port and arrive Fiber Bragg Grating FBG (60) through Transmission Fibers (30), after sensing unit and the reflection of grating grid region, reflected light signal returns along Transmission Fibers (30), arrives the 3rd port and received by spectroanalysis instrument (20) by Transmission Fibers (30) through three ports light rings (40) second ports.The grid region of Fiber Bragg Grating FBG (60) is as bio-sensing unit, be fixed in the groove of optical fiber stationary platform (50) upper surface, and when ensureing that solution to be measured pours into groove, solution to be measured is immersed and uniform liquid contact completely in surface, grid region, is blotted by solution and make grating dry in atmosphere after 15 minutes from groove.When the grid region of light wave after excessive erosion, the biotin molecule carried due to surface, grid region only plays selectivity specific adsorption to Streptavidin molecule, thus change grid region external environment refractive index, due to the effective index of fundamental mode propagated in fibre core, ambient refractive index change is very sensitive to external world, the effective refractive index of basic mode can be made to change, finally cause the reflected light centre wavelength (bragg wavelength) of Fiber Bragg Grating FBG to be drifted about.For the solution of streptavidin of variable concentrations, the Streptavidin molecular amounts that the biotin molecule on surface, grid region absorbs is different, the variations in refractive index of its near surface is also different, thus the reflected light centre wavelength drift value making spectroanalysis instrument receive is different, the concentration information of Streptavidin converts the drift value of wavelength to realize detecting the most at last.
When light wave incides Fiber Bragg Grating FBG, the centre wavelength (bragg wavelength) of reflected light can be drawn by following formula:
In formula, λ
bfor bragg wavelength,
for the effective index of fundamental mode transmitted in fibre core, Λ is screen periods.For common Fiber Bragg Grating FBG, due to the existence of covering, the effective refractive index of basic mode
not by the impact of external environment refractive index.Use hydrofluoric acid solution by the corrosion of the grid region of grating to certain degree of depth, make the diameter of covering be reduced to certain value, then ambient refractive index change is very sensitive to external world through grid region effective index of fundamental mode for light wave, now effective index of fundamental mode
nonlinear relationship can be there is with surrounding environment refractive index n.When through finishing process, the grating plating specific function rete is positioned in the solution to be measured of variable concentrations, will the Streptavidin molecule of absorption varying number, causes grid region ambient refractive index n to change different, even if the effective index of fundamental mode propagated in fibre core
change also different, then reflected light central wavelength lambda
bdrift value different, the concentration information of solution of streptavidin to be measured will be reflected in the bragg wavelengths of grating
bin change.
In the present embodiment, the output wavelength of light supply apparatus is 1200nm ~ 1650nm; The operation wavelength coverage of spectroanalysis instrument is 1200nm ~ 1650nm, and Transmission Fibers adopts Standard single-mode fiber (G.652), and core diameter is 8.2 microns, and cladding diameter is 125 microns, and numerical aperture is 0.14; Fiber Bragg Grating FBG centre wavelength is 1546.5040nm, use hydrofluorite, ammonium fluoride, pure water ratio be 1: 2: 3 hydrofluoric acid solution corrode 13.5 hours after the diameter of grating region become 6.57 μm.
Disclosed the utility model by above embodiment, but protection domain of the present utility model is not limited thereto, under the condition not departing from the present invention's design, above each component can be replaced with the similar or equivalent element that art personnel understand.
Claims (2)
1. the Streptavidin concentration sensor based on Fiber Bragg Grating FBG, it is characterized in that, described sensor is by light supply apparatus (10), spectroanalysis instrument (20), Transmission Fibers (30), three ports light rings (40), optical fiber stationary platform (50), Fiber Bragg Grating FBG (60) and solution to be measured (70) composition, the light signal output end of described light supply apparatus (10) is connected with the first port of described three ports light rings (40) by Transmission Fibers (30), second port of described three port circulators (40) is connected with described Fiber Bragg Grating FBG (60) by Transmission Fibers (30), described Fiber Bragg Grating FBG (60) is fixed by described optical fiber stationary platform (50), 3rd port of described three port circulators (40) is connected with described spectroanalysis instrument (20) by Transmission Fibers (30).
2. as claimed in claim 1 based on the Streptavidin concentration sensor of Fiber Bragg Grating FBG, it is characterized in that, the grid region of described Fiber Bragg Grating FBG (60) utilizes certain density hydrofluoric acid solution to corrode to certain depth, the diameter of covering is made to be reduced to certain value, and the grid region finishing after corrosion has the rete of specific adsorption function, described optical fiber stationary platform upper surface should have a groove, during fixed grating, grid region is in groove, during to ensure that solution to be measured pours into groove, surface, grid region is immersed solution to be measured completely and is contacted with uniform liquid.
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| CN112596174A (en) * | 2020-12-29 | 2021-04-02 | 中国科学院长春光学精密机械与物理研究所 | Composite manufacturing method of micro-nano optical fiber coupler |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112596174A (en) * | 2020-12-29 | 2021-04-02 | 中国科学院长春光学精密机械与物理研究所 | Composite manufacturing method of micro-nano optical fiber coupler |
| CN112596174B (en) * | 2020-12-29 | 2022-05-27 | 中国科学院长春光学精密机械与物理研究所 | Composite manufacturing method of micro-nano optical fiber coupler |
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Granted publication date: 20150114 Termination date: 20150929 |
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