CN204074106U - A kind of continuous equivalent pipettes the device of micro solution - Google Patents
A kind of continuous equivalent pipettes the device of micro solution Download PDFInfo
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- CN204074106U CN204074106U CN201420564976.7U CN201420564976U CN204074106U CN 204074106 U CN204074106 U CN 204074106U CN 201420564976 U CN201420564976 U CN 201420564976U CN 204074106 U CN204074106 U CN 204074106U
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Abstract
The utility model discloses the device that a kind of continuous equivalent pipettes micro solution, comprise container, be located at the suction pipe of container bottom; The top of described container is provided with pressing piston, and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position; The top of described suction pipe is connected with the bottom of container and is communicated with, above pipette tip 0.5mm place container in radius be that the diameter of Spherical Volume place of 0.5mm is without chamber wall; The described volume V being pressed into air is greater than suction pipe volume and liquor capacity sum to be pipetted, and described in be pressed into air volume V be less than 2 times of volume sums of suction pipe volume and solution to be pipetted.During use, press pressing piston at every turn, can drip and the solution that only drips, can and repeatedly operate easily; And this operation does not rely on the operative skill of operator, extremely low to the requirement of operator, thus there is positive realistic meaning.
Description
Technical field
The utility model relates to the device that a kind of continuous equivalent pipettes micro solution, pipettes the micro solution of equivalent for quantitative analysis personnel.
Background technology
In prior art, pipetting of micro solution is main by micropipettor, and the precision of micropipettor can accomplish 1 ~ 5 microlitre on the market at present.But pipette micro solution for contactless, because solution surface exists surface tension, the solution being less than droplet size (about 30 microlitre) can stick to the bottom of micropipettor and can not naturally drip.Therefore, the precision being less than droplet size is actual is invalid, and the actual volume pipetted is the volume of drippage drop.When a dropping liquid drip drip from capillary water dropper time, the volume of the drop that drips relevant with the size of the surface tension of drop and water dropper; Under same environmental condition, the surface tension of same liquid is identical.The drip droplet size of same liquid of same water dropper is equal.Therefore, in the metering process of reality, a lot of technical staff uses the number of drops of drippage and the product of liquid volume as the volume of pipetted micro solution.
But control drop dropwise drips and needs skill, operating personnel are easy to cause solution to be column ejection because of overexerting, and cause the amount of solution generation deviation pipetted.
Therefore, design the device that the continuous equivalent of a energy pipettes micro solution, make it often operate once an only drippage solution, can repeatedly operate easily again, this has realistic meaning simultaneously.Especially, in the metering process that some are special, metrological personnel needs the occasion repeating the micro solution pipetting identical volume.
Summary of the invention
Goal of the invention of the present utility model is to provide the device that a kind of continuous equivalent pipettes micro solution.
To achieve the above object of the invention, the technical solution adopted in the utility model is: a kind of continuous equivalent pipettes the device of micro solution, comprises container, is located at the suction pipe of container bottom;
The over top of described container is provided with liquid injection port, and liquid injection port place is provided with the seal cover of cooperation;
The top of described container is provided with pressing piston, and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position: pressing piston returns to original position;
The top of described suction pipe is connected with the bottom of container and is communicated with, above pipette tip 0.5 mm place container in radius be that the diameter of Spherical Volume place of 0.5 mm is without chamber wall;
The described volume V being pressed into air is greater than suction pipe volume and liquor capacity sum to be pipetted, and described in be pressed into air volume V be less than 2 times of volume sums of suction pipe volume and solution to be pipetted;
The internal diameter of described suction pipe is 0.1 ~ 2 mm, and the thickness of its tube wall is less than 0.5mm, and its volume is 0.001 ~ 0.01 mL.
Above, the over top of described container is provided with liquid injection port, and the effect of liquid injection port is used to put into the solution pipetted needed for (or injection).Liquid injection port place is provided with the seal cover of cooperation, and seal cover is used to close or open liquid injection port.
The top of described container is provided with pressing piston, and its objective is and provide a pressing mechanism, the air that can be pressed into certain volume enters in container, thus realizes a drop of liquid to ooze from suction pipe.The structure of pressing piston can adopt prior art, and such as press head adds the combining structure of back-moving spring, also can adopt and have flexible sheet metal etc.
The device of said structure is suitable for continuous equivalent, and to pipette same suction pipe be infiltrating solution.This is because be infiltrating solution with suction pipe, the volume of the drop that drips is correlated with the external diameter of suction pipe, and the desorption volume of the bubble of emerging in suction pipe is correlated with the internal diameter of suction pipe.The desorption volume of bubble is less than the volume of drop.Suction pipe drips after a dropping liquid drips and just has a bubble desorption.But be the solution of non-infiltration for same suction pipe, the volume of the drop that drips is correlated with the internal diameter of suction pipe, the desorption volume of the bubble of emerging in suction pipe is correlated with the external diameter of suction pipe.Because the top of suction pipe connects with the bottom of container, the bottom of whole container is all equivalent to suction pipe wall, the diameter relevant to bubble desorption is greater than the external diameter of true suction pipe, cause the desorption volume of bubble may much larger than the volume of the drop that drips, thus cause the dropping liquid that drips to drip rear bubble can not the situation of desorption in time.
Preferably, the volume of described container is 0.5 ~ 50 mL.
Preferably, described container is spherical.Can certainly adopt the container of other conventional shapes, preferred situation is: the cross-sectional area of container bottoms should be greater than the cross-sectional area of suction pipe, and suction pipe is positioned at container central authorities; Like this when unclamping piston, the bubble emitted up from suction pipe can be made to depart from pipette tip and successfully enter in container, the volume reduced in adding container.Can guarantee to use rear bubble to enter container smoothly and not stick on container inner wall.
Corresponding another kind of technical scheme, a kind of continuous equivalent pipettes the device of micro solution, comprises container, is located at the suction pipe of container bottom;
The over top of described container is provided with liquid injection port, and liquid injection port place is provided with the seal cover of cooperation;
The top of described container is provided with pressing piston, and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position: pressing piston returns to original position;
The top of described suction pipe is connected with the bottom of container and is communicated with, and the top of described suction pipe exceeds outside the bottom surface of container; And above pipette tip 0.5 mm place container in radius be that the diameter of Spherical Volume place of 0.5 mm is without chamber wall;
The described volume V being pressed into air is greater than suction pipe volume and liquor capacity sum to be pipetted, and described in be pressed into air volume V be less than 2 times of volume sums of suction pipe volume and solution to be pipetted;
The internal diameter of described suction pipe is 0.1 ~ 2 mm, and the thickness of its tube wall is less than 0.5mm, and its volume is 0.001 ~ 0.01 mL.
Above, the over top of described container is provided with liquid injection port, and the effect of liquid injection port is used to put into the solution pipetted needed for (or injection).Liquid injection port place is provided with the seal cover of cooperation, and seal cover is used to close or open liquid injection port.
The top of described container is provided with pressing piston, and its objective is and provide a pressing mechanism, the air that can be pressed into certain volume enters in container, thus realizes a drop of liquid to ooze from suction pipe.The structure of pressing piston can adopt prior art, and such as press head adds the combining structure of back-moving spring, also can adopt and have flexible sheet metal etc.
The device of said structure is suitable for continuous equivalent and pipettes the solution that same suction pipe is non-infiltration.The utility model by the tip designs of suction pipe be exceed container bottom surface outside, the desorption volume not receptor wall impact of bubble, thus enable bubble desorption in time.
Certainly, the device of said structure is also suitable for continuous equivalent to pipette same suction pipe is infiltrating solution.
In use, the degree of depth that suction pipe enters internal tank can not exceed height of liquid level inside container.Why suction pipe will insert internal tank is enter in container to enable bubble depart from suction pipe more smoothly, ensures that drop can drip continuously.
Preferably, the volume of described container is 0.5 ~ 50 mL.
Preferably, described container is spherical.
Working mechanism of the present utility model is: container of the present utility model is provided with pressing piston, when pressing down piston, the volume that drop can be released in suction pipe lower end is greater than one and is less than two, and departs from suction pipe with the form of drop, and remaining liquid is adsorbed on suction pipe owing to failing to reach critical size; When unclamping piston, negative pressure is become in container, now, because the volume sucking back liquid is less than the volume releasing liquid, the volume reduced is supplemented by environmental gas, and the gas supplemented becomes bubble, enters in container through suction pipe, final disengaging suction pipe upper end enters in container, now reverts to normal pressure in container.Device of the present utility model can reuse, thus realizes continuous equivalent and pipette micro solution.
Because technique scheme is used, the utility model compared with prior art has following advantages:
1, of the present utility modelly devise the device that a kind of continuous equivalent completely newly pipettes micro solution, during use, press pressing piston at every turn, can drip and the solution that only drips, can and repeatedly operate easily; And this operation does not rely on the operative skill of operator, extremely low to the requirement of operator, thus there is positive realistic meaning;
2, experiment proves, the droplet size that the utility model drips is equal, and error is minimum, continuous several times can operate, pipettes many parts of solution, be therefore extremely applicable to the occasion needing to repeat the micro solution pipetting identical volume;
3, the size of the utility model to the position of suction pipe and suction pipe and container all limits, and after ensure that pressing, bubble can enter container smoothly from suction pipe and not stick to suction pipe inwall, the volume reduced in adding container; No matter be same suction pipe be infiltrating solution or can be suitable for the solution that suction pipe is non-infiltration; Range of application is wider;
4, structure of the present utility model is simple, and cost is lower, is suitable for applying.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model embodiment one.
Fig. 2 is the structural representation of the utility model embodiment two.
1, container; 2, suction pipe; 3, seal cover; 4, piston is pressed.
Detailed description of the invention
Below in conjunction with drawings and Examples, the utility model is further described.
Embodiment one:
Shown in Figure 1, a kind of continuous equivalent pipettes the device of micro solution, comprises container 1, is located at the suction pipe 2 of container bottom;
The over top of described container is provided with liquid injection port, and liquid injection port place is provided with the seal cover 3 of cooperation;
The top of described container is provided with pressing piston 4, and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position: pressing piston returns to original position;
The top of described suction pipe is connected with the bottom of container and is communicated with;
Described container is spherical; The volume of described container is 5 mL;
The described volume V being pressed into air is 35 microlitres;
The internal diameter of described suction pipe is 0.5 mm, and suction pipe wall thickness is 0.3mm, and its volume is 5 microlitres.
The course of work of the present utility model is as follows:
(1) open the seal cover of described device, in container, inject 1 mL pure water, cover stopper;
(2) described device is vertically picked up, by lower piston, to drip a water from suction pipe;
(3) unclamping pressing piston, making environmental gas from entering in container.
When needs drip again, repeat step (2) and step (3).
In order to prove the present invention pipette solution for equal-volume solution, 1mL water is injected in the utility model, repeatedly carry out the step (2) in above-mentioned using method and step (3), until the water in container no longer drips, record the water droplet quantity of dripping.Result is as follows: carry out experiment 10 times, and the water droplet quantity of 10 times is as follows respectively: 49,49,49,49,49,49,49,49,49,49.As can be seen here, adopt liquor capacity that device of the present utility model pipettes identical, degree of repeatability is high.
Embodiment two:
Shown in Figure 2, a kind of continuous equivalent pipettes the device of micro solution, comprises container 1, is located at the suction pipe 2 of container bottom;
The over top of described container is provided with liquid injection port, and liquid injection port place is provided with the seal cover 3 of cooperation;
The top of described container is provided with pressing piston 4, and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position: pressing piston returns to original position;
The top of described suction pipe exceeds outside the bottom surface of container, exceeds 2 mm;
Described container is spherical, and the volume of described container is 5 mL;
The described volume V being pressed into air is 35 microlitres;
The internal diameter of described suction pipe is 0.5 mm, and suction pipe wall thickness is 0.3mm, and its volume is 5 microlitres.
Claims (6)
1. continuous equivalent pipettes a device for micro solution, it is characterized in that: comprise container (1), be located at the suction pipe (2) of container bottom;
The over top of described container is provided with liquid injection port, and liquid injection port place is provided with the seal cover (3) of cooperation;
The top of described container is provided with pressing piston (4), and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position: pressing piston returns to original position;
The top of described suction pipe is connected with the bottom of container and is communicated with, above pipette tip 0.5 mm place container in radius be that the diameter of Spherical Volume place of 0.5 mm is without chamber wall;
The described volume V being pressed into air is greater than suction pipe volume and liquor capacity sum to be pipetted, and described in be pressed into air volume V be less than 2 times of volume sums of suction pipe volume and solution to be pipetted;
The internal diameter of described suction pipe is 0.1 ~ 2 mm, and the thickness of its tube wall is less than 0.5mm, and its volume is 0.001 ~ 0.01 mL.
2. device according to claim 1, is characterized in that: the volume of described container is 0.5 ~ 50 mL.
3. device according to claim 1, is characterized in that: described container is spherical.
4. continuous equivalent pipettes a device for micro solution, it is characterized in that: comprise container, be located at the suction pipe of container bottom;
The over top of described container is provided with liquid injection port, and liquid injection port place is provided with the seal cover of cooperation;
The top of described container is provided with pressing piston, and it has 2 working positions: (1) presses working position: pressing piston is pressed in container, and the volume being pressed into air is V; (2) reset working position: pressing piston returns to original position;
The top of described suction pipe is connected with the bottom of container and is communicated with, and the top of described suction pipe exceeds outside the bottom surface of container; And above pipette tip 0.5 mm place container in radius be that the diameter of Spherical Volume place of 0.5 mm is without chamber wall;
The described volume V being pressed into air is greater than suction pipe volume and liquor capacity sum to be pipetted, and described in be pressed into air volume V be less than 2 times of volume sums of suction pipe volume and solution to be pipetted;
The internal diameter of described suction pipe is 0.1 ~ 2 mm, and the thickness of its tube wall is less than 0.5mm, and its volume is 0.001 ~ 0.01 mL.
5. device according to claim 4, is characterized in that: the volume of described container is 0.5 ~ 50 mL.
6. device according to claim 4, is characterized in that: described container is spherical.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201420564976.7U CN204074106U (en) | 2014-09-28 | 2014-09-28 | A kind of continuous equivalent pipettes the device of micro solution |
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CN201420564976.7U CN204074106U (en) | 2014-09-28 | 2014-09-28 | A kind of continuous equivalent pipettes the device of micro solution |
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CN204074106U true CN204074106U (en) | 2015-01-07 |
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CN201420564976.7U Expired - Fee Related CN204074106U (en) | 2014-09-28 | 2014-09-28 | A kind of continuous equivalent pipettes the device of micro solution |
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2014
- 2014-09-28 CN CN201420564976.7U patent/CN204074106U/en not_active Expired - Fee Related
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150107 Termination date: 20160928 |