CN203964970U - A kind of high precision micro quantitative instrument - Google Patents
A kind of high precision micro quantitative instrument Download PDFInfo
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- CN203964970U CN203964970U CN201420420538.3U CN201420420538U CN203964970U CN 203964970 U CN203964970 U CN 203964970U CN 201420420538 U CN201420420538 U CN 201420420538U CN 203964970 U CN203964970 U CN 203964970U
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Abstract
The utility model provides a kind of high precision micro quantitative instrument, comprise stationary platform, in stationary platform, be fixed with separation container, directly over separation container, be provided with support, on support, be fixed with nozzle and temperature-sensing element, nozzle and temperature-sensing element all can move along x, y, z all directions, and perpendicular to separation container setting; Nozzle can temperature control by gauge tap and reagent reservoir be connected; Micro quantitative determination instrument also comprises computer control system, and computer control system is all connected with gauge tap, support, is also connected with temperature-sensing element by signal processing circuit.The utility model adopts temperature-sensing element directly to monitor fluid flow, because it is highly sensitive, is therefore applicable to measure small temperature variation, and testing result is accurate.In addition, because temperature-sensing element volume is little, thermal capacity is little, fast response time, can in space and slit, measure.
Description
Technical field
The utility model relates to a kind of high precision micro quantitative instrument.
Background technology
In recent years, the new branch of science taking synthetic biology as representative had been included life science and each research direction and content relevant to mankind itself and social development in biological technical field, directly served scientific and technological progress and socio-economic development.These subjects are using the significant challenge problem that solves the aspects such as biological medicine that human kind sustainable development was faced, clean chemical industry, bioenergy, biomaterial, environmental protection and biological anti-terrorism as its current development main target; therefore there are huge application and development potentiality, in many ways paid close attention to.Someone compares the present situation of the synthetic biology conceptual phase early stage with information industry with computing machine, foretell that its research breaks through and will become the chief motivation of following biotechnology economic development, likely ignite the industrial technology revolution of a new round, really realizing 21 century is the prophesy of Century of Bio-Technology.
So urgent hope by the industrialization early of the technology of the synthesising biological of comparative maturity, is applied in national product life, promotes national product life development.
These synthesising biological technology are all accurate micro-reaction processes, the overwhelming majority is that container can hold micro liquid is as reactor, such as the container of Tissue Culture Plate, centrifuge tube and similar micro liquid (such as, gene automatic synthesizer uses synthetic post or synthesis chip), therefore synthesising biological technology has very high requirement to the injection liquid scale of construction.The traditional micro liquid injection mode in laboratory adopts liquid-transfering gun or syringe pump to inject more, and the advantage of which is that injection rate IR is accurate, can judge accurately that whether each injection rate IR is accurate.But be subject to artificial restriction, speed is slow, be not suitable for industrialized production in enormous quantities.And the robotization micro liquid injection device that can improve speed is indirectly to control injection rate IR substantially, be not to monitor intuitively injection rate IR.Common liquid driven mode has air pressure, stepper motor, piezoelectricity control.Record by great many of experiments the parameter (such as air pressure, voltage etc.) of controlling flow in advance according to type of drive, then write software by selecting parameter and time to control injection rate IR.Once therefore there be uncertain condition to occur, such as filling orifice stops up, driving circuit piece damages, inadequate etc. the situation of driving voltage occurs, injection rate IR just can not reach designated value.But control system does not obtain feedback in time still carries out next step work according to setting.The mode of indirect monitoring injection rate IR can not meet the requirement of accurate injection as can be seen here, thus urgently a kind of meet synthesising biological technology commercialization produce in enormous quantities liquid injection mode.
Summary of the invention
Problem to be solved in the utility model is to overcome the inaccurate shortcoming of injection rate IR that prior art causes due to indirect monitoring, a kind of high precision micro quantitative instrument is provided, utilize the temperature variant characteristic of resistance of temperature-sensing element, temperature-sensing element is applied in trace liquid quantitative, can not reduce under the prerequisite of injection rate, improve injection rate IR accuracy.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is: a kind of high precision micro quantitative instrument, it is characterized in that: comprise stationary platform, in described stationary platform, be fixed with separation container, directly over separation container, be provided with support, on described support, be fixed with nozzle and temperature-sensing element, nozzle and temperature-sensing element all can move along x, y, z all directions, and perpendicular to separation container setting; Described nozzle can temperature control by gauge tap and reagent reservoir be connected; Described micro quantitative determination instrument also comprises computer control system, and described computer control system is all connected with gauge tap, support, is also connected with temperature-sensing element by signal processing circuit.
Further, described temperature-sensing element is resistance thermometer clement or thermistor element.Thermistor element, by can be posistor (PTC thermistor), raises its resistance value change greatly with temperature; Or negative tempperature coefficient thermistor (NTC thermistor), described resistance thermometer clement is pt100 platinum resistance thermometer sensor.Thermal resistance is applicable to larger separation container, and thermistor is applicable to less separation container.In measurement, can select as required separation container.The thermistor elements that adopt in synthesising biological technology more.In biosynthesis technology, conventional container has the container (the synthetic post or the synthesis chip that use such as, gene automatic synthesizer) of Tissue Culture Plate, centrifuge tube and similar micro liquid.Shape, the volume of these containers are not identical, and therefore every kind of container all needs Resurvey data.
Preferably, described nozzle and temperature-sensing element all can move on support.
Preferably, described computer control system connects a display screen.
Under condition of work, measure room temperature T, and determine based on this resistance (Δ according to the decision such as temperature-sensing element, working environment of selecting) of temperature-sensing element in the time of temperature T ± Δ, be set as threshold value.Once the resistance of measuring is higher or lower than threshold value, computer control system sends instruction, closing control switch.Now flow reaches predetermined value, completes hydrojet.In order to make temperature-sensing element can measure the temperature difference, by liquid be placed in can temperature control reagent reservoir, and suitable temperature is set, make fluid temperature higher than room temperature, so that Trig control signal.
In the utility model, temperature-sensing element is as scale, for demarcating liquid capacity.To the liquid of separation container injection standard volume, then demarcate the z direction position of temperature-sensing element in advance.By test of many times, determine the not z direction position of temperature-sensing element corresponding to co-content.After work in, determine required volume by changing the z direction position of temperature-sensing element.
Advantage and the good effect that the utlity model has are: adopt temperature-sensing element directly to monitor fluid flow, because it is highly sensitive, be therefore applicable to measure small temperature variation, testing result is accurate.In addition, because temperature-sensing element volume is little, thermal capacity is little, fast response time, can in space and slit, measure.
Brief description of the drawings
Fig. 1 is the relation curve of the temperature of the resistance of NTC thermistor
Fig. 2 is the relation curve of the temperature of the resistance of pt100 platinum resistance thermometer sensor,
Fig. 3 is the structural representation of the utility model high precision micro quantitative instrument
Fig. 4 is workflow diagram of the present utility model.
In figure:
1, stationary platform; 2, separation container; 3, temperature-sensing element; 4, nozzle; 5, support.
Embodiment
Embodiment 1:
As shown in Figure 3, a kind of high precision micro quantitative instrument, comprise stationary platform 1, in stationary platform 1, be fixed with separation container 2, directly over separation container 2, be provided with support 5, on support 5, be fixed with nozzle 4 and temperature-sensing element 3, nozzle 4 and temperature-sensing element 3 all can move along x, y, z all directions, and arrange perpendicular to separation container 2; Nozzle 4 can temperature control by gauge tap and reagent reservoir be connected; Micro quantitative determination instrument also comprises computer control system, and computer control system is all connected with gauge tap, support 5, is also connected with temperature-sensing element 3 by signal processing circuit.Nozzle 4 and temperature-sensing element 3 all can move on support.Computer control system connects a display screen; Separation container can be selected as required.In biosynthesis technology, conventional container has the container (the synthetic post or the synthesis chip that use such as, gene automatic synthesizer) of Tissue Culture Plate, centrifuge tube and similar micro liquid.Shape, the volume of these containers are not identical, and therefore every kind of container all needs Resurvey data.
In the present embodiment, temperature-sensing element 3 is PTC thermistor.
In operating temperature range, the resistance-temperature characteristics of PTC thermistor can be similar to and represent with empirical formula:
R in formula
t,
expression temperature is T, T
0time resistance value, B
pfor the material constant of this kind of material.
The course of work of the present embodiment is:
1. pre-service: before work, need to carry out pre-service.
First, measure room temperature T, and determine based on this resistance of PTC thermistor in the time of temperature T ± Δ, be set as threshold value.
PTC thermistor is placed in to initial position, to the liquid of separation container injection standard volume V, then adjust the z direction position of PTC thermistor, until the resistance of PTC thermistor exceeds threshold range, now just corresponding orthobaric volume V of the z direction position of PTC thermistor.
2. select parameter: hydrojet order, reagent spouting liquid.According to course of reaction, the injection order of selective reagent and the spouting liquid of reagent.
3. hydrojet observing and controlling
The thermostatic equipment of reagent reservoir that can temperature control is opened, and ensures that solution and room temperature have certain temperature difference, are greater than Δ.Nozzle 4 is run to directly over separation container 2, according to selected parameter, set point value is adjusted to in the z direction position of PTC thermistor.Sprayed solution, liquid level rise, the resistance value information of simultaneous computer control system Real-time Collection PTC thermistor, and with threshold value comparison.If in threshold range, continue to keep current state.Rise and touch PTC thermistor once liquid, liquid raises the temperature of PTC thermistor, and the resistance value instantaneous variation of PTC thermistor, exceeds threshold value, and computer control system is just sent shutdown signal to gauge tap, stops nozzle 4 hydrojets.So far complete a hydrojet circulation.
As shown in Figure 4, in the present embodiment, PTC thermistor is converted to the variation of temperature the variation of resistance value, is transferred to signal processing circuit, through processing, the variation of resistance is converted to the variation of voltage, more further the magnitude of voltage of simulation is converted to digital signal.Digital signal is sent in computer control system and threshold value comparison, judges.If need shut-off nozzle 4, computer control system is sent instruction to gauge tap, shut-off nozzle 4.
Embodiment 2:
As shown in Figure 3, a kind of high precision micro quantitative instrument, comprise stationary platform 1, in stationary platform 1, be fixed with separation container 2, directly over separation container 2, be provided with support 5, on support 5, be fixed with nozzle 4 and temperature-sensing element 3, nozzle 4 and temperature-sensing element 3 all can move along x, y, z all directions, and arrange perpendicular to separation container 2; Nozzle 4 can temperature control by gauge tap and reagent reservoir be connected; Micro quantitative determination instrument also comprises computer control system, and computer control system is all connected with gauge tap, support 5, is also connected with temperature-sensing element 3 by signal processing circuit.Nozzle 4 and temperature-sensing element 3 all can move on support.Computer control system connects a display screen; Separation container can be selected as required.In biosynthesis technology, conventional container has the container (the synthetic post or the synthesis chip that use such as, gene automatic synthesizer) of Tissue Culture Plate, centrifuge tube and similar micro liquid.Shape, the volume of these containers are not identical, and therefore every kind of container all needs Resurvey data.
In the present embodiment, temperature-sensing element 3 is NTC thermistor.
NTC temperature-sensitive semiconductor is the oxide ceramics of spinel structure or other structures mostly, has negative temperature coefficient, and as shown in Figure 1, the relation of resistance value and temperature can approximate representation be:
R in formula
t,
expression temperature is T, T
0time resistance value, B
nfor material constant.
The course of work of the present embodiment is:
1. pre-service: before work, need to carry out pre-service.
First, measure room temperature T, and determine based on this resistance of NTC thermistor in the time of temperature T ± Δ, be set as threshold value.
NTC thermistor is placed in to initial position, to the liquid of separation container injection standard volume V, then adjust the z direction position of NTC thermistor, until the resistance of NTC thermistor exceeds threshold range, now just corresponding orthobaric volume V of the z direction position of NTC thermistor.
2. select parameter: hydrojet order, reagent spouting liquid.According to course of reaction, the injection order of selective reagent and the spouting liquid of reagent.
3. hydrojet observing and controlling
The thermostatic equipment of reagent reservoir that can temperature control is opened, and ensures that solution and room temperature have certain temperature difference, are greater than Δ.Nozzle 4 is run to directly over separation container, according to selected parameter, set point value is adjusted to in the z direction position of NTC thermistor.Sprayed solution, liquid level rise, the resistance value information of simultaneous computer control system Real-time Collection NTC thermistor, and with threshold value comparison.If in threshold range, continue to keep current state.Rise and touch NTC thermistor once liquid, liquid raises the temperature of NTC thermistor, and the resistance value instantaneous variation of NTC thermistor, exceeds threshold value, and computer control system is just sent shutdown signal to gauge tap, stops nozzle 4 hydrojets.So far complete a hydrojet circulation.
As shown in Figure 4, in the present embodiment, NTC thermistor is converted to the variation of temperature the variation of resistance value, is transferred to signal processing circuit, through processing, the variation of resistance is converted to the variation of voltage, more further the magnitude of voltage of simulation is converted to digital signal.Digital signal is sent in computer control system and threshold value comparison, judges.If need shut-off nozzle 4, computer control system is sent instruction to gauge tap, shut-off nozzle 4.
Embodiment 3:
As shown in Figure 3, a kind of high precision micro quantitative instrument, comprise stationary platform 1, in stationary platform 1, be fixed with separation container 2, directly over separation container 2, be provided with support 5, on support 5, be fixed with nozzle 4 and temperature-sensing element 3, nozzle 4 and temperature-sensing element 3 all can move along x, y, z all directions, and arrange perpendicular to separation container 2; Nozzle 4 can temperature control by gauge tap and reagent reservoir be connected; Micro quantitative determination instrument also comprises computer control system, and computer control system is all connected with gauge tap, support 5, is also connected with temperature-sensing element 3 by signal processing circuit.Nozzle 4 and temperature-sensing element 3 all can move on support.Computer control system connects a display screen; Separation container can be selected as required.In biosynthesis technology, conventional container has the container (the synthetic post or the synthesis chip that use such as, gene automatic synthesizer) of Tissue Culture Plate, centrifuge tube and similar micro liquid.Shape, the volume of these containers are not identical, and therefore every kind of container all needs Resurvey data.
In the present embodiment, temperature-sensing element 3 is pt100 platinum resistance thermometer sensor.
As shown in Figure 2, its resistance-temperature relationship can represent by following approximation relation formula,
In formula, R
t,
expression temperature is T, T
0time resistance value, α is temperature coefficient.
The course of work of the present embodiment is:
1. pre-service: before work, need to carry out pre-service.
First, measure room temperature T, and determine based on this resistance of pt100 platinum resistance thermometer sensor, in the time of temperature T ± Δ, be set as threshold value.
Pt100 platinum resistance thermometer sensor, is placed in to initial position, to the liquid of separation container injection standard volume V, then adjust the z direction position of pt100 platinum resistance thermometer sensor,, until the resistance of pt100 platinum resistance thermometer sensor, exceeds threshold range, now just corresponding orthobaric volume V of the z direction position of pt100 platinum resistance thermometer sensor.
2. select parameter: hydrojet order, reagent spouting liquid.According to course of reaction, the injection order of selective reagent and the spouting liquid of reagent.
3. hydrojet observing and controlling
The thermostatic equipment of reagent reservoir that can temperature control is opened, and ensures that solution and room temperature have certain temperature difference, are greater than Δ.Nozzle 4 is run to directly over separation container, according to selected parameter, set point value is adjusted to in the z direction position of pt100 platinum resistance thermometer sensor.Sprayed solution, liquid level rise, the resistance value information of simultaneous computer control system Real-time Collection pt100 platinum resistance thermometer sensor,, and with threshold value comparison.If in threshold range, continue to keep current state.Rise and touch pt100 platinum resistance thermometer sensor, once liquid, liquid raises the temperature of pt100 platinum resistance thermometer sensor,, and the resistance value instantaneous variation of pt100 platinum resistance thermometer sensor,, exceeds threshold value, and computer control system is just sent shutdown signal to gauge tap, stops nozzle 4 hydrojets.So far complete a hydrojet circulation.
As shown in Figure 4, in the present embodiment, pt100 platinum resistance thermometer sensor, is converted to the variation of temperature the variation of resistance value, be transferred to signal processing circuit, through processing, the variation of resistance is converted to the variation of voltage, more further the magnitude of voltage of simulation is converted to digital signal.Digital signal is sent in computer control system and threshold value comparison, judges.If need shut-off nozzle 4, computer control system is sent instruction to gauge tap, shut-off nozzle 4.
Above embodiment of the present utility model is had been described in detail, but described content is only preferred embodiment of the present utility model, can not be considered to for limiting practical range of the present invention.All equalization variation and improvement etc. of doing according to the utility model scope, within all should still belonging to this patent covering scope.
Claims (7)
1. a high precision micro quantitative instrument, it is characterized in that: comprise stationary platform, in described stationary platform, be fixed with separation container, directly over separation container, be provided with support, on described support, be fixed with nozzle and temperature-sensing element, nozzle and temperature-sensing element all can move along x, y, z all directions, and perpendicular to separation container setting; Described nozzle can temperature control by gauge tap and reagent reservoir be connected; Described micro quantitative determination instrument also comprises computer control system, and described computer control system is all connected with gauge tap, support, is also connected with temperature-sensing element by signal processing circuit.
2. micro quantitative determination instrument according to claim 1, is characterized in that: described temperature-sensing element is resistance thermometer clement or thermistor element.
3. micro quantitative determination instrument according to claim 2, is characterized in that: described thermistor element is PTC thermistor or NTC thermistor.
4. micro quantitative determination instrument according to claim 2, is characterized in that: described resistance thermometer clement is pt100 platinum resistance thermometer sensor.
5. micro quantitative determination instrument according to claim 1, is characterized in that: described nozzle and temperature-sensing element all can move on support.
6. micro quantitative determination instrument according to claim 1, is characterized in that: described computer control system connects a display screen.
7. micro quantitative determination instrument according to claim 1, is characterized in that: described separation container can be Tissue Culture Plate, centrifuge tube or micro liquid container.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104132697A (en) * | 2014-07-28 | 2014-11-05 | 中国科学院天津工业生物技术研究所 | High-accuracy micro-quantifying instrument and quantifying method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104132697A (en) * | 2014-07-28 | 2014-11-05 | 中国科学院天津工业生物技术研究所 | High-accuracy micro-quantifying instrument and quantifying method thereof |
| CN104132697B (en) * | 2014-07-28 | 2018-01-16 | 中国科学院天津工业生物技术研究所 | A kind of high precision micro quantitative instrument and its quantitative approach |
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