CN203960223U - Tissue Culture Plate - Google Patents

Tissue Culture Plate Download PDF

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Publication number
CN203960223U
CN203960223U CN201420342664.1U CN201420342664U CN203960223U CN 203960223 U CN203960223 U CN 203960223U CN 201420342664 U CN201420342664 U CN 201420342664U CN 203960223 U CN203960223 U CN 203960223U
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CN
China
Prior art keywords
ware
base
culture plate
tissue culture
slide block
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Expired - Fee Related
Application number
CN201420342664.1U
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Chinese (zh)
Inventor
叶菲
王彦
朱庆
彭云东
刘玲燕
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Sichuan Agricultural University
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Sichuan Agricultural University
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Priority to CN201420342664.1U priority Critical patent/CN203960223U/en
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Publication of CN203960223U publication Critical patent/CN203960223U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model relates to a kind of Tissue Culture Plate, and it comprises culture dish and ware lid; Culture dish comprises base, core body and ware body; Base is provided with at least one pair of chute; Core body has at least two groups; The bottom of core body is embedded at the bottom land of chute, and stage casing passes outside base one sidewall to a side horizontal-extending and extending part, and top passes and passes position to the top face of base and is provided with fixture block; From end face, the inside to base is evenly provided with cell cultures hole to ware body; One group of opposing sidewalls of ware lid is provided with groove; Groove one end is opening-like, and the relative the other end is closed, and bottom side cell wall is also provided with slip ditch; The top of core body is through slip ditch and fixture block coupling is embedded in groove, to realize ware lid and ware body assembly connection.The utility model structure design is simple, rationally, cost of manufacture is low, does not change the ventilation property of Tissue Culture Plate, facilitate again Tissue Culture Plate taking or mobile, also can reduce in culture plate cell contaminated.

Description

Tissue Culture Plate
Technical field
The utility model relates to a kind of cell cultures utensil, relates in particular to a kind of Tissue Culture Plate.
Background technology
Tissue Culture Plate is a kind of cell cultures vessel of commonly using in cell experiment, its material is generally plastics, mostly be disposable use, but not link closely between now commercially available cell cultures plate structure middle cover and ware body, take or the process of migratory cell culture plate in lid be easy to be opened, cause extraneous bacterium etc. to enter cell cultures hole and cause cell contamination.
Given this, in prior art, mainly adopted the Tissue Culture Plate of following four kinds of structure designs:
The first Tissue Culture Plate is comprised of lid and ware body two portions, and wherein lid and identical can the fastening with it of ware phosphor bodies part size, be buckled on base; Ware body inside is provided with cell cultures hole (being generally: 6 holes, 12 holes, 24 holes, or 96 holes) and runs through ware body, is cell cultures region.The culture hole edge of some Tissue Culture Plates also protrudes a ware body part, and is provided with the fastening indenture identical with culture hole aperture at the internal surface of lid top cover, can be chimeric with the edge that culture hole is protruded after fastening lid, but not fastening.Gap and the external world that the cell of ware body inside can fasten by lid and ware body carry out gaseous interchange, due to lid back-off, " L " type edge in lid and the formation of ware body produces air-negative-pressure, can stop the air-flow that carries dust to pass through edge, because the microorganisms such as bacterium are generally attached on dust in air, therefore prevented that the pollutents such as bacterium from entering cell cultures region.Yet, it is that lid can not link closely with ware body that the first Tissue Culture Plate exists a major defect, can cause like this in the process of mobile or the Tissue Culture Plate of taking, lid is easy to be raised, cause the outside air that carries the pollutents such as bacterium to enter in culture hole and cause cell contamination, increased the risk of cell cultures failure.
The second Tissue Culture Plate is comprised of ware body and lid, in ware body, be provided with the cell cultures hole for cell cultures, ware body two sides periphery is provided with the handle of conveniently taking, and two positive peripheries are respectively arranged with a snap close I, this snap close I consists of lockhole and magnet, and magnet is arranged in lockhole; On the lid two medial surfaces position corresponding with snap close I, be provided with snap close II and be similarly a magnet.When fastening lid, the snap close II of lid medial surface is relative with the snap close I position in external week of ware, thereby the magnet that it is inner and the magnet on lid suck the object that reaches fastening lid mutually, in ware body two sides, be provided with two handles and facilitate taking of Tissue Culture Plate simultaneously.Yet, fastening Tissue Culture Plate in the second, lid is to realize by the magnetism between two magnets, although can reach the object of fastening lid when the magnetism between magnet is enough powerful, but in Tissue Culture Dish inside, also can produce steady magnetic field, and steady magnetic field all can produce very strong detrimental effect to many cells as the growth of cranial nerve cell; On this medical disposable material, magnet is installed, has also increased the cost that Tissue Culture Plate is made simultaneously.
The third cell cultures plate structure is comprised of cover and base plate, and wherein cover and base plate link together by coupling shaft and torsion spring, and torsion spring set is on coupling shaft; On cover, be also provided with hasp simultaneously, on base plate, be provided with the snap fit part coordinating with it; While fastening lid, hasp and hasp accessory fasten, thereby realize fixing to cover, and during uncap, torsion spring is controlled cover and is opened into certain angle, thereby can change to the cell in base plate liquid or processing.Yet, the third Tissue Culture Plate, although facilitated fastening and the operation of lid, lid is fixed on ware body and reduces the handiness that the lid of knowing clearly is placed, and while uncapping, cover inner surface can only be exposed in air, and can not detain on the table, if there is like this liquid splash in operating process, cover inner surface will very easily be sticked to, while again fastening lid, may, not only by polluting but also bringing cell cultures district into, improve the contaminated risk of cell; Meanwhile, torsion spring being installed also can make the cost of manufacture of Tissue Culture Plate increase.
The 4th kind of Tissue Culture Plate is comprised of upper cover and base plate, and wherein, upper cover is a platy structure, and its two ends, left and right have respectively two buckles that can bend downwards, two protruding fastenings that can arrange with base plate two ends; Upper cover is slidably arranged in the sliding mouthful groove of both sides, base plate upper end; Base plate interior is provided with cell cultures hole for cell cultures; Wherein a sliding mouthful groove is comprised of two material laths identical with base plate, in specific implementation process, right/left pull upper cover can progressively expose cell cultures hole, cell is changed to liquid, process etc., after end of operation, upper cover is pushed back to zero position, and the buckle of detaining two ends fastens the projection of itself and base plate both sides, thereby reach the object of fastening lid.Yet, the 4th kind of Tissue Culture Plate, taked a kind of structure of pull upper cover really to facilitate to a certain extent operation, and be provided with buckle at lid two ends, also played the object of fixed cover, but the buckle arranging on lid is a kind of external structure, when lid fastens, buckle surfaces externally and internally all contacts with extraneous, easily adsorb various bacterium dusts etc., can be with the top, pull process cell cultures district of lid when opening the buckle of buckle pulling cover period of the day from 11 p.m. to 1 a.m one end, now just very easily bacterium dust of its absorption etc. is brought in culture hole, caused cell contamination.
In sum, be necessary prior art further perfect.
Summary of the invention
In order to overcome the above problems, the utility model provides a kind of structure design simple, reasonable, and cost of manufacture is low, does not change the ventilation property of Tissue Culture Plate, facilitate again taking or moving of Tissue Culture Plate, also can reduce the Tissue Culture Plate of the contaminated risk of cell in culture plate.
The utility model is achieved through the following technical solutions:
Above-mentioned Tissue Culture Plate, comprises that culture dish and coupling are installed in the ware lid on described culture dish; Described culture dish comprises base, core body and ware body; One group of relative side of described base is arranged with at least one pair of chute; Described core body is that coupling and symmetry are installed at least two groups in described pair of sliding groove; Described core body can slide in described chute, its bottom coupling is embedded at the bottom land of described chute, stage casing passes outside described base one sidewall to a side horizontal-extending and extending part, and top passes and passes position to the top face of described base and is provided with fixture block; Described ware body is located at the end face of described base and is connected to one with described base, and from end face, the inside to described base is evenly provided with cell cultures hole to described ware body; Described ware lid coupling covers on described ware body, in one group opposing sidewalls, is arranged with groove; Described groove one end is opening-like, and the relative the other end is closed, and bottom side cell wall is also provided with slip ditch; The top coupling of described core body is through described slip ditch and described fixture block coupling is embedded in described groove, to realize described ware lid and ware body assembly connection.
Described Tissue Culture Plate, wherein: the outside surface of described core body is coated with frosted layer; The cell body internal surface of described groove is also coated with frosted layer; The frosted layer contact of the frosted layer of described core outer surface and the cell body internal surface of described groove is connected, so that described ware covers and ware body chimeric connection tightly.
Described Tissue Culture Plate, wherein: described chute be cross section be " on " cell body of character form structure, by the inner side, bottom near described base and the first cell body arranging in horizontal direction, on described the first cell body, middle side part longitudinally arranges and connects the second cell body of described base top surface extremely and the 3rd cell body to the outer side wall lateral direction penetrating setting of described base forms from described the second cell body one middle side part.
Described Tissue Culture Plate, wherein: described core body be " on " character form structure, by the first horizontally disposed slide block, form from make progress straight the second slide block extending to form and horizontal-extending forms outside described the second slide block middle part one side direction the 3rd slide block of described the first slider top; Described the first slide block coupling is embedded in described the first chute; Described the second slide block coupling is embedded in described the second cell body and top passes to the top outer of described base, and described fixture block is fixedly arranged on one end that described the second slide block passes to described base top outside; Described the 3rd slide block coupling is embedded in described the 3rd cell body and passes outside described base one sidewall.
Described Tissue Culture Plate, wherein: one end that described the 3rd slide block passes outside described base one sidewall can be used as push-and-pull button and uses, and to control described core body, in described chute, moves.
Described Tissue Culture Plate, wherein: the top of described the second slide block can be mated through described slip ditch.
Described Tissue Culture Plate, wherein: the rectangular plate body structure of described base; Described ware body integral body is rectangular structure.
Beneficial effect:
The utility model Tissue Culture Plate structure design is simple, compact, use simple, convenient, cost of manufacture is low, wherein the bottom of core body coupling is embedded at the bottom land of chute, can realize the assembly connection of core body and base, the stage casing of core body passes outside base one sidewall to a side horizontal-extending and extending part, one end that core body passes outside base one sidewall can be used as push-and-pull button and uses, by the controlled coremaking body of push-and-pull button, in chute, move, by push-and-pull button, make core body and ware cover corresponding groove tightly chimeric or depart from, thereby realize the fastening of ware lid or open, meanwhile, the top of core body passes and passes position to the top face of base and is provided with the fixture block matching with groove, by being connected of fixture block and groove, can realize ware lid and ware body assembly connection, moreover the cell body internal surface of the outside surface of core body and groove is coated with frosted layer, the contact of the frosted layer of the frosted layer of core outer surface and the cell body internal surface of groove is connected, can make ware cover and ware body chimeric connection tightly, therefore, the structure design of whole culture plate, does not change the ventilation property of Tissue Culture Plate, facilitate again Tissue Culture Plate taking or mobile, also can reduce in culture plate cell contaminated, be suitable for propagation and employment.
Accompanying drawing explanation
Fig. 1 is the sectional view of the utility model Tissue Culture Plate;
Fig. 2 is the enlarged view of a-quadrant in Fig. 1 of the utility model Tissue Culture Plate;
Fig. 3 is the structural representation of the culture dish of the utility model Tissue Culture Plate;
Fig. 4 is the structural representation of the ware lid of the utility model Tissue Culture Plate;
Fig. 5 is that the culture dish of the utility model Tissue Culture Plate removes the sectional view after core body;
Fig. 6 is the enlarged view in B region in Fig. 5 of the utility model Tissue Culture Plate.
Embodiment
As shown in Figures 1 to 6, the utility model Tissue Culture Plate, comprises culture dish 1 and ware lid 2.
This culture dish 1 integral body is rectangular structure, and it comprises base 11, core body 12 and ware body 13.
Wherein, the rectangular plate body structure of this base 11, one group relative side is arranged with pair of sliding groove 14, this pair of sliding groove 14 be all along the width setting of ware body 13 and be cross section be all " on " cell body of character form structure, each chute 14 is by the first cell body 141, the second cell body 142 and the 3rd cell body 143 form, wherein, this first cell body 141 is near this base 11 inner sides, bottom and setting in horizontal direction, this second cell body 142 is that middle side part longitudinally arranges and connects to the end face of base 11 always on the first cell body 141, the 3rd cell body 143 is the outer side wall lateral direction penetrating settings to base 11 from the second cell body 142 1 middle side parts.
These core body 12 couplings are installed in the chute 14 of base 11, and outside surface scribbles frosted layer; This core body 12 has two groups, and this two core assemblies body 12 is in the symmetrical chute 14 that is installed in 11 1 groups of relative dual-sides of base and can be along the interior slip of chute 14, and in the present embodiment, every core assembly body 12 all has a pair of; Each core body 12 be all " on " character form structure, by the first horizontally disposed slide block 121, form from make progress extending vertically the second slide block 122 forming and horizontal-extending forms outside the second slide block 122 middle part one side direction the 3rd slide block 123 of the first slide block 121 center of top; These the first slide block 121 couplings are embedded in the first chute 141 of chute 14; These the second slide block 122 couplings are embedded in the second cell body 142 and pass to base 11 top outer, wherein, the fixture block 124 that one end that this second slide block 122 passes to base 11 top outer is also installed with, in the present embodiment, this fixture block 124 is rectangular structure; The 3rd slide block 123 couplings are embedded in the 3rd cell body 143 and outside the sidewall of base 11 and pass, wherein, one end that the 3rd slide block 123 passes to the sidewall outside of base 11 can be used as push-and-pull button and uses, by the controlled coremaking body 12 of push-and-pull button in the interior movement of chute 14.
These ware body 13 integral body are rectangular parallelepiped platy structure, and itself and base 11 are connected to one, and are fixedly arranged on the end face of base 11; Wherein, this ware body 13 is provided with cell cultures hole 131, and this cell cultures hole 131 is equally distributed a plurality of along these ware body 13 end faces, and each cell cultures hole 131 is all from the end face of ware body 13, to extend to the inside of base 11; In the present embodiment, this cell cultures hole 131 is along ware body 13 end faces equally distributed six.
This ware covers 2 integral body and is rectangular parallelepiped lid, and its coupling covers on the ware body 13 of this culture dish 1, wherein, is arranged with the two group grooves 21 corresponding with chute 14 couplings of base 11 in one group of opposing sidewalls of this ware lid 2,
It is a pair of that every group of groove 21 all has, and the cell body internal surface of each groove 21 is coated with frosted layer, and in the present embodiment, the equal integral body of each groove 21 is rectangular structure; Simultaneously, each equal one end of groove 21 is opening-like, relative the other end closed, bottom side cell wall is equipped with slip ditch 211, the fixture block 124 of the cell body of this groove 21 and core body 12 matches, this slip ditch 211 matches with the second slide block 122 of core body 12, this ware lid 2 is connected and ware body 13 assembly connections by groove 21 and the fixture block 124 of core body 12, wherein, the frosted layer contact of the frosted layer of groove 21 cell body internal surfaces and core body 12 outside surfaces is connected so that ware cover 2 with ware body 13 chimeric connection tightly.
Principle of work of the present utility model:
During use, ware lid 2 is fastened on ware body 13, groove 21 positions that ware covers in 2 two lateral walls are corresponding with chute 14, after fastening, groove 21 is positioned at the second cell body 142 tops of chute 14, by promoting the 3rd slide block 123 of each core body 12, drive core body 12 in the interior movement of chute 14, make the second slide block 122 through the slip ditch 211 on groove 21, thereby drive the fixture block 124 of outside surface frosted at the second slide block 122 tops tightly chimeric with the groove 21 that ware covers 2 upper inner surface frosteds, thereby realize fastening to ware lid 2; While uncapping, promote the 3rd slide block 123 and drive core body 12 to move, make the fixture block 124 at the second slide block 122 tops depart from the groove 21 on ware lid 2, can open ware lid 2.
The utility model structure design is simple, rationally, it is simple, convenient to use, cost of manufacture is low, does not change the ventilation property of Tissue Culture Plate, facilitate again Tissue Culture Plate taking or mobile, also can reduce in culture plate cell contaminated, be suitable for propagation and employment.
The above; it is only preferred embodiment of the present utility model; be not the utility model to be done to the restriction of any other form, and according to any modification or equivalent variations that technical spirit of the present utility model is done, still belong to the utility model scope required for protection.

Claims (7)

1. a Tissue Culture Plate, comprises that culture dish and coupling are installed in the ware lid on described culture dish; It is characterized in that: described culture dish comprises base, core body and ware body;
One group of relative side of described base is arranged with at least one pair of chute;
Described core body is that coupling and symmetry are installed at least two groups in described pair of sliding groove; Described core body can slide in described chute, its bottom coupling is embedded at the bottom land of described chute, stage casing passes outside described base one sidewall to a side horizontal-extending and extending part, and top passes and passes position to the top face of described base and is provided with fixture block;
Described ware body is located at the end face of described base and is connected to one with described base, and from end face, the inside to described base is evenly provided with cell cultures hole to described ware body;
Described ware lid coupling covers on described ware body, in one group opposing sidewalls, is arranged with groove; Described groove one end is opening-like, and the relative the other end is closed, and bottom side cell wall is also provided with slip ditch;
The top coupling of described core body is through described slip ditch and described fixture block coupling is embedded in described groove, to realize described ware lid and ware body assembly connection.
2. Tissue Culture Plate as claimed in claim 1, is characterized in that: the outside surface of described core body is coated with frosted layer; The cell body internal surface of described groove is also coated with frosted layer; The frosted layer contact of the frosted layer of described core outer surface and the cell body internal surface of described groove is connected, so that described ware covers and ware body chimeric connection tightly.
3. Tissue Culture Plate as claimed in claim 1, it is characterized in that: described chute be cross section be " on " cell body of character form structure, by the inner side, bottom near described base and the first cell body arranging in horizontal direction, on described the first cell body, middle side part longitudinally arranges and connects the second cell body of described base top surface extremely and the 3rd cell body to the outer side wall lateral direction penetrating setting of described base forms from described the second cell body one middle side part.
4. Tissue Culture Plate as claimed in claim 3, it is characterized in that: described core body be " on " character form structure, by the first horizontally disposed slide block, form from make progress straight the second slide block extending to form and horizontal-extending forms outside described the second slide block middle part one side direction the 3rd slide block of described the first slider top;
Described the first slide block coupling is embedded in described the first chute;
Described the second slide block coupling is embedded in described the second cell body and top passes to the top outer of described base, and described fixture block is fixedly arranged on one end that described the second slide block passes to described base top outside;
Described the 3rd slide block coupling is embedded in described the 3rd cell body and passes outside described base one sidewall.
5. Tissue Culture Plate as claimed in claim 4, is characterized in that: one end that described the 3rd slide block passes outside described base one sidewall can be used as push-and-pull button and uses, and to control described core body, in described chute, moves.
6. Tissue Culture Plate as claimed in claim 4, is characterized in that: the top of described the second slide block can be mated through described slip ditch.
7. Tissue Culture Plate as claimed in claim 1, is characterized in that: the rectangular plate body structure of described base; Described ware body integral body is rectangular structure.
CN201420342664.1U 2014-06-24 2014-06-24 Tissue Culture Plate Expired - Fee Related CN203960223U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420342664.1U CN203960223U (en) 2014-06-24 2014-06-24 Tissue Culture Plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420342664.1U CN203960223U (en) 2014-06-24 2014-06-24 Tissue Culture Plate

Publications (1)

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CN203960223U true CN203960223U (en) 2014-11-26

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434312A (en) * 2016-09-22 2017-02-22 东莞市联洲知识产权运营管理有限公司 Microorganism culture dish
CN107236663A (en) * 2016-03-28 2017-10-10 杭州高正医学科技有限公司 Culture medium storing unit
WO2022205820A1 (en) * 2021-03-30 2022-10-06 上海睿钰生物科技有限公司 Culture device and culture method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107236663A (en) * 2016-03-28 2017-10-10 杭州高正医学科技有限公司 Culture medium storing unit
CN106434312A (en) * 2016-09-22 2017-02-22 东莞市联洲知识产权运营管理有限公司 Microorganism culture dish
WO2022205820A1 (en) * 2021-03-30 2022-10-06 上海睿钰生物科技有限公司 Culture device and culture method

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20141126

Termination date: 20150624

EXPY Termination of patent right or utility model