CN203960183U - A kind of darkroom connecting joint for CCD camera - Google Patents
A kind of darkroom connecting joint for CCD camera Download PDFInfo
- Publication number
- CN203960183U CN203960183U CN201420245214.0U CN201420245214U CN203960183U CN 203960183 U CN203960183 U CN 203960183U CN 201420245214 U CN201420245214 U CN 201420245214U CN 203960183 U CN203960183 U CN 203960183U
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- China
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- darkroom
- ccd camera
- connecting joint
- flange plug
- closed level
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a kind of darkroom connecting joint for CCD camera, and the darkroom connecting joint of CCD camera comprises flange plug and closed level; Flange plug is cast by nonmagnetic metal, and closed level is arranged on the upper surface of flange plug, and closed level is step-like flange plug surface the outwardly convex of being arranged on, and is provided with the fixed head matching with CCD camera head on flange plug; The utility model is by the setting of sealing-ring on the connecting joint of darkroom and set ring, make to be easy to install between the mount pad of sequence testing chip of CCD camera and reaction warehouse and mate formation darkroom, step-like sealing-ring has strengthened obturation effect simultaneously, stopping property excellence, make darkroom environment more airtight, effectively prevent external light pollution, reduce ground unrest, and seal life is long.
Description
Technical field
The utility model relates to DNA sequencing technologies field, is specifically related to a kind of darkroom connecting joint for CCD camera.
Background technology
Tetra-sodium sequencing technologies is by the enzyme cascade chemiluminescence reaction in 4 kinds of enzymatic same reaction systems.The principle of tetra-sodium sequencing technologies is: after primer and template DNA annealing, under the synergy of archaeal dna polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and four kinds of enzymes of apyrase (Apyrase), the release coupling of the polymerization of each dNTP on primer and an optical signal is got up, by detecting release and the intensity of light, reach the object of the real time measure DNA sequence dna.The reaction system of tetra-sodium sequencing technologies is made up of reaction substrate, strand to be measured, sequencing primer and four kinds of enzymes.Reaction substrate is 5 '-phosphinylidyne sulfuric acid (adenosine-5 '-phosphosulfate, APS) and fluorescein (luciferin).
Take turns in sequencing reaction at each, in reaction system, only add a kind of deoxynucleotide triphosphoric acid (dNTP), if it
Can just with the next base pairing of DNA profiling, can be under the effect of archaeal dna polymerase, add 3 ' end of sequencing primer to, discharge the tetra-sodium (PPi) of a molecule simultaneously.Under the effect of ATP sulfurylase, the PPi of generation can be with APS in conjunction with forming ATP, and under the catalysis of luciferase, the ATP of generation can, with fluorescein in conjunction with forming oxyluciferin, produce visible ray again simultaneously.Can obtain a special detected peaks by Weak light detection device and process software, the height of peak value is directly proportional with the base number matching.If the dNTP adding can not with the next base pairing of DNA template, above-mentionedly instead would not occur, just there is no detected peaks yet.In reaction system, remaining dNTP and residual a small amount of ATP degrade under the effect of Apyrase.After last round of reaction completes, add another kind of dNTP, above-mentioned reaction is repeated, can read DNA sequence information accurately according to the peak value figure obtaining.
Integrated operation flow process is described below: DNA sample by fragmentation after, application is built storehouse reagent and is added joint, strand is caught, be bonded to microballoon, microemulsion PCR amplification, breakdown of emulsion liquid, acquisition is based upon the DNA library on microballoon, application add model by laies such as the enzymes of library and sequencing reaction needs to the sequence testing chip with micro reaction pool, sequence testing chip and sequencing reagent are mounted on main frame, start order-checking program by controlling computer according to module number and position, sequencing reaction is carried out in automatization, the data transmission producing is to data analysis computer, after completing order-checking, computation analysis software carries out image processing, sequence is read, mass analysis, the work such as sequence assembly, finally obtain the sequence information of DNA sample.
In order-checking process, on sequence testing chip, there is chemical reaction, produce visible ray, by CCD(Charge Couple
Device) camera catches the optical signal that sequencing reaction produces, and can obtain needed order-checking information.Because order-checking signal is the optical signal of extremely faint visible light wave range, therefore need to ensure enough dark environment of between CCD camera and the mount pad of reaction warehouse formation, not contaminated with the signal that ensures to check order, can intactly be received by CCD camera.
In prior art, as the patent application in the darkroom between the patent No. be 201210164839.X the mount pad for sequence testing chip and CCD camera, the front end of CCD camera is provided with annular recesses, directly be affixed to the airtight connection of flange collar on the mount pad of the sequence testing chip on reaction warehouse, make bonded to each other to obtain the optical signal of sequencing reaction by annular recesses and flange collar of the fibre faceplate of CCD camera and sequence testing chip, between CCD camera and the mount pad of reaction warehouse, form the darkroom of closure, the shortcoming of prior art is, annular recesses mates not easy to operate with flange collar, it is improper easily to cause mating, leave crack, make to be easily subject between the fibre faceplate of CCD camera and sequence testing chip the interference of visible ray, normally carrying out and efficiency of impact order-checking.
Utility model content
For the problems referred to above, the utility model provides a kind of darkroom connecting joint for CCD camera, by the setting of sealing-ring and set ring on the connecting joint of darkroom, make to be easy to install mate between the mount pad of sequence testing chip of CCD camera and reaction warehouse to form darkroom, the while, step-like sealing-ring strengthened obturation effect, stopping property excellence, make darkroom environment more airtight, effectively prevent external light pollution, reduce ground unrest, and seal life is long.
For addressing the above problem, the technical solution adopted in the utility model: the described darkroom connecting joint for CCD camera comprises the closed level on flange plug and flange plug surface; Flange plug is cast by nonmagnetic metal, and closed level is step-like flange plug surface the outwardly convex of being arranged on, and is provided with the camera gim peg matching with CCD camera head on flange plug; The darkroom connecting joint of described pedestal and CCD camera matches, on permanent seat, be provided with the annular recesses corresponding with closed level on flange plug surface, the wall of annular recesses is step shape, with be step-like closed level and mate, on permanent seat, be provided with the setting circle road of a circle projection, the sequence testing chip central position on the corresponding permanent seat in setting circle Dao center is corresponding.
As preferably, the flange plug surface of the described darkroom connecting joint for CCD camera is provided with a circle to recessed location circular groove, matches with the setting circle road on permanent seat.
As preferably, the closed level of the described darkroom connecting joint for CCD camera adopts quality of rubber materials to form.
As preferably, described closed level is step-like, and step number is 3-5 rank.
The beneficial effects of the utility model: the utility model provides a kind of darkroom connecting joint for CCD camera, by the setting of sealing-ring and set ring on the connecting joint of darkroom, make to be easy to install between the mount pad of sequence testing chip of CCD camera and reaction warehouse and mate formation darkroom, step-like sealing-ring has strengthened obturation effect simultaneously, stopping property excellence, makes darkroom environment more airtight, effectively prevents external light pollution, reduce ground unrest, and seal life is long.
brief description of the drawings:
Fig. 1 is the structure iron of the utility model connecting joint;
Fig. 2 is the structure iron of the utility model permanent seat;
In figure: 1-darkroom connecting joint, 2-flange plug, 3-closed level, 4-permanent seat, 5-camera gim peg, 6-location circular groove, 7-annular recesses, 8-setting circle road.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described further, to facilitate the technical staff to understand.
As shown in Figure 1 and Figure 2, the described darkroom connecting joint 1 for CCD camera comprises the closed level 3 on flange plug 2 and flange plug surface; Flange plug 2 is cast by nonmagnetic metal, and the employing of nonmagnetic metallic substance can effectively prevent the electromagnetic interference in use procedure, improves the precision of order-checking.
Closed level 3 is step-like flange plug 2 surface the outwardly convexs that are arranged on, and will on permanent seat 4, be provided with and the lip-deep closed level 3 of flange plug 2, on permanent seat 4, be also provided with corresponding annular recesses 7, the wall of annular recesses 7 is step shape, with be step-like closed level 3 and mate, can further increase like this tightness of combination between permanent seat 4 and darkroom connecting joint 1, further improve the light stopping property in darkroom, its sealing effectiveness excellence, make darkroom environment more airtight, effectively prevent external light pollution, when ensureing card sequence testing chip and being positioned at the fibre faceplate coupling of front end of CXD camera, not disturbed by extraneous light, thereby reduce ground unrest, ensure the obtaining of order-checking signal of high s/n ratio.
On flange plug 2, be provided with the camera gim peg 5 matching with CCD camera head, flange plug 2 is fixed on CCD camera, be easy to dismounting simultaneously, easy to use.
Simultaneously, due to the setting of the hierarchic structure of closed level 3 and annular recesses 7, increase the difficulty of matching of closed level 3 and annular recesses 7 in actual use procedure, annular recesses 7 is mated not easy to operate with closed level 3, therefore exist, on permanent seat 4, be provided with the setting circle road 8 of a circle projection, the central position of the sequence testing chip on the corresponding permanent seat 4 in center in setting circle road 8 is corresponding, be provided with a circle to recessed location circular groove 6 on flange plug 2 surfaces of darkroom connecting joint 1 again, match with the setting circle road 8 on permanent seat 4, as guiding, be convenient to make both accurately to connect, for attended operation is provided convenience.
The utility model provides a kind of darkroom connecting joint for CCD camera, by the setting of sealing-ring and set ring on the connecting joint of darkroom, make to be easy to install between the mount pad of sequence testing chip of CCD camera and reaction warehouse and mate formation darkroom, step-like sealing-ring has strengthened obturation effect simultaneously, stopping property excellence, makes darkroom environment more airtight, effectively prevents external light pollution, reduce ground unrest, and seal life is long.
The utility model describes by specific implementation process, in the situation that not departing from the utility model scope, can also carry out various conversion and be equal to replacement the utility model patent, therefore, the utility model patent is not limited to disclosed specific implementation process, and should comprise the whole embodiments that fall within the scope of the utility model Patent right requirement.
Claims (5)
1.
?for a darkroom connecting joint for CCD camera, it is characterized in that: the described darkroom connecting joint for CCD camera comprises flange plug and closed level; Flange plug is cast by nonmagnetic metal, and closed level is arranged on the upper surface of flange plug, and closed level is step-like flange plug surface the outwardly convex of being arranged on, and is provided with the camera gim peg matching with CCD camera head on flange plug.
2.
?a kind of darkroom, darkroom for CCD camera is connected and fixed pedestal, it is characterized in that: the darkroom connecting joint of described permanent seat and CCD camera matches, on permanent seat, be provided with the annular recesses corresponding with closed level on flange plug surface, the wall of annular recesses is step shape, with be step-like closed level and mate, on permanent seat, be provided with the setting circle road of a circle projection, the sequence testing chip central position on the corresponding permanent seat in setting circle Dao center is corresponding.
3.
?darkroom connecting joint for CCD camera according to claim 1, is characterized in that: the flange plug surface of the described darkroom connecting joint for CCD camera is provided with a circle to recessed location circular groove, matches with the setting circle road on permanent seat.
4.
?darkroom connecting joint for CCD camera according to claim 1, is characterized in that: the closed level of the described darkroom connecting joint for CCD camera adopts quality of rubber materials to form.
5.
?darkroom connecting joint for CCD camera according to claim 1, is characterized in that: described closed level is step-like, step number is 3-5 rank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420245214.0U CN203960183U (en) | 2014-05-14 | 2014-05-14 | A kind of darkroom connecting joint for CCD camera |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420245214.0U CN203960183U (en) | 2014-05-14 | 2014-05-14 | A kind of darkroom connecting joint for CCD camera |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203960183U true CN203960183U (en) | 2014-11-26 |
Family
ID=51920792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420245214.0U Expired - Fee Related CN203960183U (en) | 2014-05-14 | 2014-05-14 | A kind of darkroom connecting joint for CCD camera |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203960183U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017005075A1 (en) * | 2015-07-08 | 2017-01-12 | 上海清流生物医药科技有限公司 | Method and apparatus for collecting signal, and method and apparatus for tracking cell by using light sensitive chip |
| CN106434342A (en) * | 2016-09-24 | 2017-02-22 | 成都测迪森生物科技有限公司 | Cell culture dish |
| CN106497785A (en) * | 2016-09-24 | 2017-03-15 | 成都测迪森生物科技有限公司 | A kind of Universal Cell culture dish |
-
2014
- 2014-05-14 CN CN201420245214.0U patent/CN203960183U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017005075A1 (en) * | 2015-07-08 | 2017-01-12 | 上海清流生物医药科技有限公司 | Method and apparatus for collecting signal, and method and apparatus for tracking cell by using light sensitive chip |
| CN106434342A (en) * | 2016-09-24 | 2017-02-22 | 成都测迪森生物科技有限公司 | Cell culture dish |
| CN106497785A (en) * | 2016-09-24 | 2017-03-15 | 成都测迪森生物科技有限公司 | A kind of Universal Cell culture dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141126 Termination date: 20150514 |
|
| EXPY | Termination of patent right or utility model |