CN203949922U - Detect the enzyme-linked immunologic detecting kit of non-food coloring adjuvant tonyred content - Google Patents
Detect the enzyme-linked immunologic detecting kit of non-food coloring adjuvant tonyred content Download PDFInfo
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- CN203949922U CN203949922U CN201320848185.2U CN201320848185U CN203949922U CN 203949922 U CN203949922 U CN 203949922U CN 201320848185 U CN201320848185 U CN 201320848185U CN 203949922 U CN203949922 U CN 203949922U
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- reagent bottle
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- groove
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- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 title claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 19
- 230000001900 immune effect Effects 0.000 title claims abstract description 13
- 239000002671 adjuvant Substances 0.000 title claims abstract description 12
- 239000000576 food coloring agent Substances 0.000 title claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 58
- 238000002965 ELISA Methods 0.000 claims abstract description 17
- 239000012530 fluid Substances 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 239000000758 substrate Substances 0.000 claims abstract description 15
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical compound [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000012086 standard solution Substances 0.000 claims abstract description 7
- 238000002372 labelling Methods 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- 239000004033 plastic Substances 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims description 5
- 239000000427 antigen Substances 0.000 claims description 4
- 102000036639 antigens Human genes 0.000 claims description 4
- 108091007433 antigens Proteins 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 description 4
- 240000004160 Capsicum annuum Species 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 241000238370 Sepia Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- VQLYBLABXAHUDN-UHFFFAOYSA-N bis(4-fluorophenyl)-methyl-(1,2,4-triazol-1-ylmethyl)silane;methyl n-(1h-benzimidazol-2-yl)carbamate Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1.C=1C=C(F)C=CC=1[Si](C=1C=CC(F)=CC=1)(C)CN1C=NC=N1 VQLYBLABXAHUDN-UHFFFAOYSA-N 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 230000000215 hyperchromic effect Effects 0.000 description 1
- 239000004434 industrial solvent Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000010721 machine oil Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model relates to a kind of enzyme-linked immunologic detecting kit that detects non-food coloring adjuvant tonyred content, comprises box body and base, and base is plastic feet, and base top surface is provided with rectangular parallelepiped groove, is wherein placed with ELISA Plate; Be provided with region one, region two, region three and region four on the right side of rectangular parallelepiped groove, in region one, be provided with 6 identical right cylinder grooves one, wherein be placed with standard solution reagent bottle, in region two, be provided with 5 tetragonal body grooves, be placed with respectively antibody working fluid, substrate A liquid reagent bottle, substrate B liquid reagent bottle, stop buffer reagent bottle and enzyme labeling thing reagent bottle, in region three, be provided with 1 right cylinder groove three, wherein be placed with antibody working fluid reagent bottle, in region four, be provided with 1 right cylinder groove four, be wherein placed with concentrated cleaning solution reagent bottle; The bottom of all grooves is all in same level; Between base top surface and box body, be placed with instructions.This kit has the advantages such as easy and simple to handle, expense is cheap, sensitivity is high.
Description
Technical field
The utility model relates to food inspection field, is specifically related to a kind of enzyme-linked immunologic detecting kit that detects non-food coloring adjuvant tonyred content.
Background technology
Tonyred is a kind of chemical staining agent, not food additives.In its chemical analysis, contain a kind of compound that is naphthalene, this material has azo structure, has carcinogenicity because the character of this chemical constitution has determined it, and the liver kidney organ of human body is had to obvious toxic action.Tonyred belongs to chemical industry coloring agent, is mainly used in oil, machine oil and other some industrial solvents, and object is to make it hyperchromic, also adding lustre to for footwear, floor etc.
At present, on market, there are some producers tonyred to be added in the food such as thick chilli sauce, chilli oil, chilli powder, catsup, tomato dip, therefore, a kind of can fast detecting tonyred and the enzyme-linked immunologic detecting kit of content very necessary to people's health.
Utility model content
The purpose of this utility model is to provide a kind of enzyme-linked immunologic detecting kit that detects non-food coloring adjuvant tonyred content, it has the advantages such as easy and simple to handle, expense is cheap, sensitivity is high, can carry out field quick detection, be applicable to great amount of samples rapid screening etc.
The purpose of this utility model is to be achieved through the following technical solutions:
A kind of enzyme-linked immunologic detecting kit that detects non-food coloring adjuvant tonyred content, comprise box body and the base that is positioned at box body, described base is plastic feet, and the left side of base top surface is provided with rectangular parallelepiped groove, in described rectangular parallelepiped groove, is placed with ELISA Plate, right side at described rectangular parallelepiped groove is provided with region one, region two, region three and region four, in described region one, be provided with 6 identical right cylinder grooves one, in described right cylinder groove one, be placed with 6 bottles of standard solution reagent bottles, in described region two, be provided with 5 tetragonal body grooves, in described tetragonal body groove, place respectively 1 bottle of antibody working fluid, 1 bottle of substrate A liquid reagent bottle, 1 bottle of substrate B liquid reagent bottle, 1 bottle of stop buffer reagent and 1 bottle of enzyme labeling thing reagent bottle, in described region three, be provided with 1 right cylinder groove three, in described right cylinder groove three, be placed with 1 bottle of antibody working fluid reagent bottle, in described region four, be provided with 1 right cylinder groove four, in described right cylinder groove four, be placed with 1 bottle of concentrated cleaning solution reagent bottle, the bottom of all described grooves is all in same level, between described base top surface and box body, be placed with instructions.
Further, described ELISA Plate is 96 holes or 48 hole ELISA Plate, and pre-coated on the capillary strip of described ELISA Plate have a tonyred coupled antigen.
Further, in described antibody working fluid reagent bottle, contain tonyred antibody.
Further, described substrate A liquid reagent bottle and substrate B liquid reagent bottle are sepia reagent bottle; Described standard solution reagent bottle, stop buffer reagent bottle and antibody working fluid reagent bottle are white reagent bottle.
Further, the height of all described grooves is 1/3～1/2 of the ELISA Plate that will place and reagent bottle height.
Further, described base with box body for can separate type being connected.
The utility model provides a kind of enzyme-linked immunologic detecting kit that detects non-food coloring adjuvant tonyred content, and the beneficial effects of the utility model are mainly:
1. the structural design of this kit is more reasonable, reagent bottle is placed more steady, and reagent body separates by box, makes it safer, and stores and use more conveniently, and in addition, this kit rationally utilizes space, is designed beautifully, and has certain market using value;
2. this kit is easy and simple to handle, expense is cheap, highly sensitive, can carry out field quick detection, be applicable to great amount of samples rapid screening etc.
Brief description of the drawings
With reference to the accompanying drawings the utility model is described in further detail below.
Fig. 1 is the structural representation of base in the enzyme-linked immunologic detecting kit of the detection non-food coloring adjuvant tonyred content described in the utility model embodiment.
In figure:
2, base; 11, region one; 12, region two; 13, region three; 14, region four; 21, right cylinder groove one; 22, tetragonal body groove; 23, right cylinder groove three; 24, right cylinder groove four; 25, rectangular parallelepiped groove; 31, standard solution reagent bottle; 32, antibody working fluid; 33, substrate A liquid reagent bottle; 34, concentrated cleaning solution reagent bottle; 35, ELISA Plate; 36, substrate A liquid reagent bottle; 37, substrate B liquid reagent bottle; 38, stop buffer reagent bottle; 39, enzyme labeling thing reagent bottle.
Embodiment
As shown in Figure 1, a kind of enzyme-linked immunologic detecting kit that detects non-food coloring adjuvant tonyred content described in the utility model embodiment, comprise box body and the base 2 that is positioned at box body, described base 2 is plastic feet, the left side of 2 end faces is provided with rectangular parallelepiped groove 25, in described rectangular parallelepiped groove 25, is placed with ELISA Plate 35, right side at described rectangular parallelepiped groove 25 is provided with region 1, region 2 12, region 3 13 and region 4 14, in described region 1, be provided with 6 identical right cylinder grooves 1, in described right cylinder groove 1, be placed with 6 bottles of standard solution reagent bottles 31, in described region 2 12, be provided with 5 tetragonal body grooves 22, in described tetragonal body groove 22, place respectively 1 bottle of antibody working fluid 32, 1 bottle of substrate A liquid reagent bottle 36, 1 bottle of substrate B liquid reagent bottle 37, 38 and 1 bottle of enzyme labeling thing reagent bottle 39 of 1 bottle of stop buffer reagent bottle, in described region 3 13, be provided with 1 right cylinder groove 3 23, in described right cylinder groove 3 23, be placed with 1 bottle of antibody working fluid reagent bottle 33, in described region 4 14, be provided with 1 right cylinder groove 4 24, in described right cylinder groove 4 24, be placed with 1 bottle of concentrated cleaning solution reagent bottle 34, the reeded bottom protrusion of institute all, in same level, can steadily be placed, between described base 2 end faces and box body, be placed with instructions.
Further, substrate A liquid reagent bottle 36 and substrate B liquid reagent bottle 37 are sepia reagent bottle; Described standard solution reagent bottle 31, stop buffer reagent bottle 38 and antibody working fluid reagent bottle 33 are white reagent bottle, in the antibody working fluid of antibody working fluid reagent bottle 33, contain tonyred specific antibody.The height of all described grooves is 1/3～1/2 of the ELISA Plate 35 that will place and reagent bottle height, and ELISA Plate 35 is 1 × 96 orifice plate (12 × 8 hole).Described base 2 with box body for can separate type being connected.
When concrete enforcement, as required region 1, region 2 12, region 3 13 and region 4 14 use colors are marked, easy to use, and be not easy to obscure reagent bottle; Reagent bottle cap and body also can be designed to different colors according to actual needs.
When concrete use, according to indirect competitive ELISA method, pre-coated coupled antigen on the capillary strip of ELISA Plate 35, coupled antigen pre-coated on residue tonyred in sample and capillary strip is competed its antibody, add after enzyme labeling thing, with tmb substrate colour developing, sample light absorption value becomes negative correlation with the content of its contained tonyred, relatively can draw the content of residue tonyred with typical curve.
The utility model is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under enlightenment of the present utility model; no matter but do any variation in its shape or structure; every have identical with a application or akin technical scheme, within all dropping on protection domain of the present utility model.
Claims (3)
1. one kind is detected the enzyme-linked immunologic detecting kit of non-food coloring adjuvant tonyred content, comprise box body and the base (2) that is positioned at box body, it is characterized in that: described base (2) with box body for can separate type being connected, described base (2) is plastic feet, the left side of base (2) end face is provided with rectangular parallelepiped groove (25), is placed with ELISA Plate (35) in described rectangular parallelepiped groove (25), right side at described rectangular parallelepiped groove (25) is provided with region one (11), region two (12), region three (13) and region four (14), in described region one (11), be provided with identical 6 right cylinder grooves one (21), in described right cylinder groove one (21), be placed with 6 bottles of standard solution formula agent bottles (31), in described region two (12), be provided with 5 tetragonal body grooves (22), in described tetragonal body groove (22), place respectively 1 bottle of antibody working fluid (32), 1 bottle of substrate A liquid reagent bottle (36), 1 bottle of substrate B liquid reagent bottle (37), 1 bottle of stop buffer reagent bottle (38) and 1 bottle of enzyme labeling thing reagent bottle (39), in described region three (13), be provided with 1 right cylinder groove three (23), in described right cylinder groove three (23), be placed with 1 bottle of antibody working fluid reagent bottle (33), in described region four (14), be provided with 1 right cylinder groove four (24), in described right cylinder groove four (24), be placed with 1 bottle of concentrated cleaning solution reagent bottle (34), the bottom of all described grooves is all in same level, the height of all described grooves is 1/3～1/2 of the ELISA Plate (35) that will place and reagent bottle height, between described base (2) end face and box body, be placed with instructions.
2. the enzyme-linked immunologic detecting kit of detection non-food coloring adjuvant tonyred content according to claim 1, it is characterized in that: described ELISA Plate (35) is 96 holes or 48 hole ELISA Plate, pre-coated on the capillary strip of described ELISA Plate have a tonyred coupled antigen.
3. the enzyme-linked immunologic detecting kit of detection non-food coloring adjuvant tonyred content according to claim 1, is characterized in that: in described antibody working fluid reagent bottle (33), contain tonyred antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320848185.2U CN203949922U (en) | 2013-12-17 | 2013-12-17 | Detect the enzyme-linked immunologic detecting kit of non-food coloring adjuvant tonyred content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320848185.2U CN203949922U (en) | 2013-12-17 | 2013-12-17 | Detect the enzyme-linked immunologic detecting kit of non-food coloring adjuvant tonyred content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203949922U true CN203949922U (en) | 2014-11-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320848185.2U Expired - Lifetime CN203949922U (en) | 2013-12-17 | 2013-12-17 | Detect the enzyme-linked immunologic detecting kit of non-food coloring adjuvant tonyred content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203949922U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114104482A (en) * | 2021-11-05 | 2022-03-01 | 西南大学 | Kit for separating and decomposing cellulose microorganisms for teaching and application thereof |
-
2013
- 2013-12-17 CN CN201320848185.2U patent/CN203949922U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114104482A (en) * | 2021-11-05 | 2022-03-01 | 西南大学 | Kit for separating and decomposing cellulose microorganisms for teaching and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: SHENZHEN BAOANKANG BIOTECHNOLOGY Co.,Ltd. Person in charge of patents Document name: payment instructions |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: SHENZHEN BAOANKANG BIOTECHNOLOGY Co.,Ltd. Person in charge of patents Document name: Notice of Termination of Patent Rights |
|
| DD01 | Delivery of document by public notice |