CN203821054U - Production equipment for electrostatic spinning fibrous membrane - Google Patents
Production equipment for electrostatic spinning fibrous membrane Download PDFInfo
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- CN203821054U CN203821054U CN201420143200.8U CN201420143200U CN203821054U CN 203821054 U CN203821054 U CN 203821054U CN 201420143200 U CN201420143200 U CN 201420143200U CN 203821054 U CN203821054 U CN 203821054U
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- Prior art keywords
- spray
- syringe
- production equipment
- spray nozzle
- electrostatic spinning
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Landscapes
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
Abstract
The utility model relates to the technical field of electrostatic spinning, in particular to production equipment for an electrostatic spinning fibrous membrane as well as a method utilizing the equipment to prepare the electrostatic spinning fibrous membrane. The production equipment for the electrostatic spinning fibrous membrane also comprises a nozzle device capable of spraying a solution containing reinforcing materials or a solution containing growth factors. The production equipment is simple in structure, and the nozzle device is additionally arranged for combining electrostatic spinning with spraying, so that a nano fibrous membrane with high strength can be obtained by preparation, or a nano fibrous membrane with a better medicine carrying effect can be obtained by preparation. After the production equipment disclosed by the utility model is adopted for producing the fibrous membrane, the problem that the existing nano fibrous membrane is not high in strength or the loaded active factors are easily inactivated can be solved, and the production equipment has good application prospect.
Description
Technical field
The electrostatic spinning technique field that the utility model relates to, more specifically, relates to a kind of production equipment of electrospun fibers film.
Background technology
Electrostatic spinning is that a kind of charged Polymer Solution or melt of utilizing flows and the spining technology of distortion preparation nanometer to micron-scale polymer fiber in electrostatic field, the polymer fiber of preparing with respect to conventional method, the advantage such as the standby fiber of electro-spinning has that diameter is little, porosity is high, porosity communication, specific area are large, and its equipment easily assemble, easy to operate.Meanwhile, electrostatic spinning nano fiber support can be conducive to adhesion and the propagation of cell in form and the anthropomorphic body n cell epimatrix (ECM) of structure patrix, and favourable condition is created in the regeneration that can be histoorgan.Just because of above-mentioned advantage, electrospun fibers film is had broad application prospects at medical fields such as bionical, organizational projects.But the nanofiber film dynamic performance obtaining by Static Spinning is poor, having limited it needs the application in the regeneration field of more powerful support in tendon, ligament, muscle, hernia etc.
The method that can improve electrostatic spinning fiber film-strength has a variety of, and they have advantage separately, also have the limitation of its application.As electrostatic spinning process carried out to many-sided improvement: a, designing different collection methods, as drum-type, liquid collection, dull and stereotyped collection, exchange electrode etc.; B, spinning process is improved, as used additional electric field, design multi-end spinning etc.; C, optimization spinning technique, comprise regulation voltage, receiving range, concentration of dope, spinning solution flow velocity etc.But above-mentioned method is to improving the mechanical property DeGrain of nano fibrous membrane.
Also have in addition physical thermal to process.As mentioned in patent ZL 200710056878.7, after being dissolved, PVDF carries out electrospinning, and obtain superfine fibre film, and the superfine fibre film of two-layer or multilayer continuous heat seal on heat seal device is processed, obtain the PVDF tunica fibrosa of good mechanical properties; In addition inorganic material and polymer dissolution are formed to blend solution in same solvent in addition, after the nano fibrous membrane that electrostatic spinning obtains, carry out high-temperature calcination, obtain having high-intensity nano fibrous membrane.Although the heat treated mode of physics, can greatly improve the mechanical property of nano fibrous membrane, the heat resistance of basic polymer is had relatively high expectations, and the nano fibrous membrane after Overheating Treatment is generally partially hard, porosity and specific area can reduce accordingly.Also can adopt physical blending as cospinning or blending, but its spinning condition is harsher, can not be suitable for all polymer.And if take electrostatic spinning nano fiber film and other film direct combinations, under stressing conditions, easily there is lamination, described in CN 101787651 A.
And take chemical crosslinking, curing mode to improve intensity, be also more common.As provided a kind of method by polycondensation to obtain high-intensity nano fibrous membrane in CN101139746A, polyamic acid Static Spinning obtains PAA nonwoven fabric, and high-temperature polycondensation becomes polyimide nanofiber membrane.In addition, can adopt directly initator or crosslinking agent and mixed with polymers are dissolved and carried out electrospinning, or form the solution that dipping contains initator or crosslinking agent after electrospun fiber membrane, then crosslinked, solidify and obtain having high-intensity nano fibrous membrane.On the one hand, the aspect of chemical modification needs polymer to have corresponding functional group, as ester group, carboxyl, hydroxyl, amino etc., can not carry out reinforcement for all polymer nanofibre films, porosity, the specific area of the nano fibrous membrane after being cross-linked on the other hand, solidifying can reduce accordingly, and nano fibrous membrane has the possibility of hardening simultaneously.
No matter above-mentioned which kind of method, is all to adopt original electrospinning device, increases that follow-up processing step realizes after preparing static spinning membrane, its complicated operation, and technique is difficult to control.Design a kind of can simplifying the operation, obtain the production equipment of the electrospun fiber membrane that intensity is high simultaneously, there is practical significance.
Summary of the invention
The utility model is at least one defect overcoming described in above-mentioned prior art, a kind of production equipment of electrospun fibers film is provided, it is simple in structure, by the spray nozzle device of setting up, make Static Spinning and spraying combination, reinforcing material and/or active factors are dispersed in to electrospinning membrane fiber internal stent, obtain the nano fibrous membrane that there is high strength or contain highly active active factors; Further, the method of utilizing this equipment to prepare electrospun fibers film is provided, it is compound that the method can have some material and the nano fibrous membrane of excellent mechanical performances, for nano fibrous membrane provides mechanics, supports, and makes to obtain having high porosity, the nano fibrous membrane of high-specific surface area; Possess higher mechanical strength simultaneously, can be widely used in hernia, shoulder sleeve, tendon, the reparation of ligament equivalent damage.Meanwhile, the solution that the method can also contain active factors by some is combined better with nano fibrous membrane, reduces the inactivation risk of growth factor.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is: a kind of production equipment of electrospun fibers film, comprise electrospinning liquid liquid feed device, the electrospinning liquid spinning appts being connected with electrospinning liquid liquid feed device, electrospinning liquid spinning appts is provided with spray silk syringe needle; Also comprise receiving system and the first HV generator, the second HV generator, receiving system is provided with the reception roller of rotatable reception spray silk syringe needle ejection fiber, the first HV generator is electrically connected to spray silk syringe needle, and the second HV generator is electrically connected to reception roller;
Also comprise spray nozzle device, described spray nozzle device is provided with spray nozzle, spray nozzle and the angled setting of spray silk syringe needle.
In this programme, spray nozzle device can arrange a plurality of, so spray nozzle also can be for a plurality of, like this can be so that composition abundant and regulation and control spraying ingredient.
Spray nozzle is with the distance that receives roller for being less than or equal to 100mm, and the proterties of the characteristic of spray area and solution, pressure, distance, nozzle etc. are closely related, and distance is shorter, spray area is less, owing to receiving roller, be cambered surface, receiving area is little, and excessive distance is unfavorable for receiving.So need command range lower than 100mm.
Preferably, spray nozzle is 2 ~ 100mm with the distance that receives roller.
Under the effect of the first HV generator and the second HV generator, because spray silk syringe needle is with high pressure, and receive roller strap, connect opposite polarity high pressure or ground connection, therefore between spray silk syringe needle and reception roller, there is pressure reduction, drop is subject to the comprehensive function of surface tension, gravity and electric field force at syringe needle place, after leaving syringe needle, split into fiber, received roller receives.Regulation voltage level changes electrostatic spinning spray silk syringe needle and the Electric Field Distribution that receives roller, to reach the object that regulates properties of product.And by the spray nozzle device of setting up, make Static Spinning and spraying combination, and reinforcing material or active factors are dispersed in to electrospinning membrane fiber internal stent by spraying coating process, obtain the nano fibrous membrane of modification.
Further, described spray nozzle is all located at and is received roller periphery with spray silk syringe needle, and the angle between spray nozzle and spray silk syringe needle is 90 °～180 °.
This equipment is to reequip by existing electrospinning device, directly over its syringe needle of general electrospinning device is placed as, equipment after repacking, the position of syringe needle can solderless wrapped connection to receive roll shaft in rotary moving, it can be placed on receiving system directly over, can also solderless wrapped connection receive roller rotation arbitrarily angled, the angle preferably forming between spray nozzle and electrostatic spinning spray silk syringe needle is 90 ~ 180 to spend.
Further, described electrospinning liquid liquid feed device comprises the front pipe of micro pump, syringe, syringe pump, and before micro pump, pipe one end connects a spray syringe needle, and the other end connects syringe, and syringe connects syringe pump.By connecting the syringe of pipe before micro pump, be arranged on syringe pump, and the speed advancing is set by syringe pump.Start syringe pump, manage before feed liquid is squeezed into micro pump from syringe, by pipe before micro pump, arrive a spray silk syringe needle, due to the effect of surface tension, electric field force, gravity etc., at syringe needle place, form taylor cone.
Further, described electrospinning liquid spinning appts comprises support, for driving the reciprocating drive unit of support, and a spray silk syringe needle is installed on support, and support is connected with drive unit.This electrospinning liquid spinning appts can be placed on receiving system directly over, also can be placed on the both sides of receiving system.And this spinning appts can solderless wrapped connection receiving apparatus Rotate 180 degree, can be from rotation directly over receiving system to both sides.
Further, described spray nozzle device also connects spray pressure generation device.Described spray nozzle device also comprises spraying syringe, control valve, the first flexible pipe, the second flexible pipe, spray nozzle is connected with control valve, control valve is connected with spraying syringe by the first flexible pipe, in spraying syringe, there is the space of loading spray coating liquor, second flexible pipe one end connects spraying syringe, and the other end connects spray pressure generation device.By containing reinforcing material or packing in sealable spraying syringe containing the solution of active factors, with flexible pipe, connect syringe and control valve, on control valve, there is flow regulator, can regulate the flow of spraying.The other end sealing of spraying syringe is also connected with Pressure generator.Pressure generator utilizes the spraying syringe that plunger displacement pump, membrane pump etc. give to connect to increase pressure, to can be good at atomization during solution ejection.
Further, described receiving system also comprises rotary electric machine, and rotary electric machine is connected with reception roller.Receiving system is to form by receiving roller, bracing frame, shaft coupling and rotary electric machine.Reception roller is fixed on bracing frame by bearing.Connect shaft coupling and be connected and fixed with rotary electric machine, by driven by motor, being rotated.
A preparation method for electrospun fibers film, the production equipment that application is described, comprises the following steps:
S1. prepare electrospinning solution: preparation is for the solution of electrostatic spinning;
S2. the solution of step S1 is packed in syringe;
S3. prepare spray coating liquor: described spray coating liquor is the solution/emulsion that contains reinforcing material or the solution that contains growth factor, and described spray coating liquor is transferred in spray equipment;
S4. carry out electrostatic spinning and spraying operation, the startup of spraying operation, for start with electrostatic spinning or restart after electrostatic spinning starts simultaneously, terminates as and stops with electrostatic spinning simultaneously or stop before electrostatic spinning stops in spraying operation;
S5. post processing: the sample obtaining after step S4 is completed is immersed in suitable solution, is dried after removing residual solvent;
S6. cutting, packing, sterilizing.
Particularly, step S1. is according to the inherent viscosity of different polymer, molecular weight and molecular weight distribution, and preparation is applicable to the toughness solution of electrostatic spinning;
In step S1, the mass concentration of polymer in solution is 5 ~ 25%.
In step S2, electrostatic spinning parameter area is: spinning voltage: 10kv ~ 30kv; Spinning solution fltting speed: 2.5ml/h ~ 10ml/h; Receiving range: 10cm ~ 20cm; Control temperature between 20 ℃ ~ 35 ℃, humidity is between 35% ~ 70%.
Step S3 is according to the performance of material, is dissolved in and in suitable solvent, forms solution or add dispersant to make it be uniformly dispersed into emulsion, or required growth factor is dissolved in suitable solvent.In step S3, the mass concentration of reinforcing material in solution or emulsion is 5 ~ 35%; Growth factor is 0.1 ~ 15% in the mass concentration of solution.
Preferably, in step S5, the sample obtaining after first adopting heat treatment mode to complete S4 carries out preliminary treatment, after remove portion residual solvent, then is dipped in suitable solution.Residual solvent can be more effectively removed in processing like this, improves the quality of tunica fibrosa.
Preferably, heat treated temperature is 35 ℃ ~ 100 ℃, and heat treatment time is 1 ~ 5 day;
In step S6, described baking temperature is 35 ℃ ~ 60 ℃, and the processing time is 1 ~ 3 day.
Utilize process units described in the utility model, the polymer dissolution for electrostatic spinning, at suitable organic solvent, by the mode of electrostatic spinning, is formed to nano fibrous membrane; Simultaneously, to there is high-intensity material dissolves in suitable solvent, form the solution of homogeneous, or add dispersant to form emulsion, the solution that contains reinforcing material is packed in spraying syringe (having connected flush coater), and good seal, utilize the booster pump of the forms such as plunger displacement pump, membrane pump to the spraying syringe pressurization that installs solution, then through high-pressure hose, be transported to spray nozzle place, after the instantaneous atomization in spray nozzle place, spray to receiving system.Owing to receiving roller state in rotating always in the process receiving, when turning to electrostatic spinning spray silk syringe needle dead ahead, can be wound around nanofibers; When turn to spray nozzle over against time, the material after atomization can be deposited on nanofibers, nanofibers and supporting material are alternately piled up like this, form the nano fibrous membrane with higher-strength.
At present, there is a large amount of research to show, by the mode of electrostatic spinning, growth factor can be carried on nano-bracket, but because most polymer is when carrying out Static Spinning, need to be dissolved in organic solvent, and often organic solvent can destroy the activity of growth factor, this has brought certain difficulty and requirement also to the loading of growth factor.Utilize process units described in the utility model, by thering is the solution that contains active factors, pack in spraying syringe (having connected flush coater), and good seal, utilize the booster pump of the forms such as plunger displacement pump, membrane pump to the spraying syringe pressurization that installs solution, then through high-pressure hose, be transported to spray nozzle place, after the instantaneous atomization in spray nozzle place, spray to receiving system.Owing to receiving roller state in rotating always in the process receiving, when turning to electrostatic spinning spray silk syringe needle dead ahead, can be wound around nanofibers; When turn to spray nozzle over against time, material after atomization can be deposited on nanofibers, nanofibers and active factors are alternately piled up like this, active factors and nanofiber are combined effectively, due in preparation process, growth factor is what to separate with polymer, can avoid growth factor because be subject to the risk that the impact of polymer solvent causes inactivation, and this cohesive process does not have harsh requirement to growth factor solution condition, be almost that all growth factors all can load.
As a kind of preferred version, when needs preparation contains the tunica fibrosa of active factors, step S4. preferably, after the unlatching of electrostatic spinning and before finishing, sprays operation, can guarantee that like this active factors is wrapped in the inside of polymer nanofibre film support completely.
In the utility model, polymeric material as electrostatic spinning comprises: polylactide and its copolymer, pla-pcl and copolymer thereof, PGA and copolymer thereof, polyethylene glycol, polyethylene terephthalate, polytetrafluoroethylene (PTFE), polyvinylidene fluoride, polymethyl methacrylate, PHBV, poly butyric capronate, polyphosphate, polyurethane acid anhydride, polyesteramide, polyvinyl alcohol, polyoxy ethane, poly-to two evil ketone, polyurethanes, Merlon, collagen, gelatin, shitosan, modification of chitosan, starch, cellulose, modified cellulose, fibrin, silk-fibroin, the peptide polymer of elastin mimicry, alginic acid, chondroitin sulfate, heparin, agar, glucan, alginic acid.
Above-mentioned polymeric material is dissolved in suitable solvent, forms electrospinning liquid, just can obtain tunica fibrosa by electrostatic spinning technique.These solvents can be the mixture of formic acid, acetic acid, ethanol, acetone, dimethyl formamide, dimethylacetylamide, oxolane, dimethyl sulfoxide (DMSO), hexafluoroisopropanol, trifluoroethanol, carrene, chloroform, methyl alcohol, diox, HFC-143a, trifluoroacetic acid, water or their arbitrary proportions.
Described reinforcing material can comprise: the two oxazoles of carbon fiber, polyethylene fiber, polypropylene fibre, polyacrylonitrile fibre, polyester fiber, vinal, polyphtalamide fiber, wool, nylon, silk, artificial silk, polyparaphenylene's benzo, polyphenylene sulfide fibre, glass fibre, polyamide-based, polyimides, polymethyl methacrylate, polyvinyl acetate, polyvinyl chloride.Above-mentioned reinforcing material is dissolved in suitable solvent, forms solution, or add dispersant, form emulsion.These solvents can be the mixture of formic acid, acetic acid, ethanol, acetone, dimethyl formamide, dimethylacetylamide, oxolane, dimethyl sulfoxide (DMSO), hexafluoroisopropanol, trifluoroethanol, carrene, chloroform, methyl alcohol, diox, HFC-143a, trifluoroacetic acid, water or their arbitrary proportions.Described dispersant is: the natural polymers such as sodium orthophosphate, many sodium metaphosphates, gelatin, algin, albumen, the cellulose derivative such as methylcellulose, hydroxypropyl methylcellulose, the polyvinyl alcohol of partial alcoholysis, the sodium salt of the copolymer of maleic anhydride and styrene or vinyl acetate, the synthetic polymers such as polyacrylate or copolymer.One or several mixtures of polyvinylpyrrolidone, poly(ethylene oxide), polyvinyl alcohol, polymethyl methacrylate, polyisobutene, polyester, fatty acid etc.
Described growth factor can comprise:
Stem cell factor, vascular endothelial growth factor (VEGF), blood platelet class growth factor (PDGF, ODGF), EGF, EGF, TGF, one or several in (TGF α and TGF β), fibroblast growth factor (α FGF, β FGF), insulin-like growth factor (IGF-I, IGF-II), nerve growth factor (NGF), interleukins class growth factor (IL-1, IL-1, IL-3 etc.), erythropoietin (EPO), colony stimulating factor (CSF) etc.
Compared with prior art, the utlity model has following beneficial effect:
The utility model discloses a kind of new electrospun fibers film process units, utilizes the spray equipment of this device, can prepare more easily the electrospun fibers film of modification; Particularly, utilize spray equipment can make reinforcing material be combined better with static spinning membrane, obtain the high nano fibrous membrane of mechanical strength, utilize spray equipment can make active factors and matrix nano tunica fibrosa separate configuration, sequential fuel injection, avoids the risk of active factors inactivation; Meanwhile, because spraying coating process is to carry out in the process of electrostatic spinning, spray coating liquor can be combined closely with nanofibers, can be because of tensile deformation difference, and produce tear, the phenomenon such as layering.
Accompanying drawing explanation
Fig. 1 is embodiment 1 overall structure schematic diagram of the present utility model.
Fig. 2 is embodiment 2 overall structure schematic diagrames of the present utility model.
The specific embodiment
Accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent; For better explanation the present embodiment, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product; To those skilled in the art, in accompanying drawing some known configurations and explanation thereof may to omit be understandable.In accompanying drawing, describe position relationship only for exemplary illustration, can not be interpreted as the restriction to this patent.
Embodiment 1
As shown in Figure 1, a kind of equipment of electrospun fibers film, comprises electrospinning liquid liquid feed device 1, the electrospinning liquid spinning appts 2 being connected with electrospinning liquid liquid feed device 1, and electrospinning liquid spinning appts 2 is provided with spray silk syringe needle 21; Also comprise receiving system 5 and the first HV generator 6, the second HV generator 7, receiving system 5 is provided with the reception roller 51 of rotatable reception spray silk syringe needle 21 ejection fibers, the first HV generator 6 is electrically connected to spray silk syringe needle 21, and the second HV generator 7 is electrically connected to reception roller 51;
Wherein, the spray nozzle device 3 that also comprises the solution that can spray the solution that contains reinforcing material or contain growth factor.
In the present embodiment, under the effect of the first HV generator 6 and the second HV generator 7, because spray silk syringe needle 21 is with high pressure, and receive roller 51 with high pressure or the ground connection of opposite polarity, therefore there is pressure reduction in spray silk syringe needle 21 and 51, roller of reception, and drop is subject to the comprehensive function of surface tension, gravity and electric field force at syringe needle place, after leaving syringe needle, split into fiber, received roller 51 receives.Regulation voltage level changes electrostatic spinning spray silk syringe needle 21 and the Electric Field Distribution that receives roller 51, to reach the object that regulates properties of product.And by the spray nozzle device 3 of setting up, make Static Spinning and spraying combination, obtain the nano fibrous membrane of modification.
In Fig. 1, spray nozzle device 3 is provided with spray nozzle 31, and spray nozzle 31 is all located at and is received roller 51 periphery with spray silk syringe needle 21, and the angle between spray nozzle 31 and spray silk syringe needle 21 is 90 °～180 °.
In the present embodiment, in Fig. 1, spray nozzle device 3 quantity are one, and spray nozzle 31 quantity are also one, still, a plurality of spray nozzle devices 3 can be set, and are convenient to composition abundant and regulation and control spraying ingredient.
In the present embodiment, angle is 90 °, this equipment is to reequip by electrospinning device, directly over its syringe needle of general electrospinning device is placed as, equipment after repacking, the position of syringe needle can be in rotary moving, it can be placed on receiving system 5 directly over, can also solderless wrapped connection receive roller 51 90-degree rotations, be placed on the both sides that receive roller, in the present embodiment, spray silk syringe needle 21 be placed in receiving system 5 directly over, spray nozzle 31 is installed on the right side that receives roller 51, electrostatic spinning syringe needle and nozzle are all perpendicular to receiving roller, and the angle forming between spray nozzle 31 and electrostatic spinning spray silk syringe needle 21 is 90 degree.
Electrospinning liquid liquid feed device 1 comprises the front pipe 11 of micro pump, syringe 12, syringe pump 13, and before micro pump, silk syringe needles 21 are sprayed in pipe 11 one end connections, and the other end connects syringe 12, and syringe 12 connects syringe pumps 13.By connecting, manage 11 syringe 12 before micro pump and be arranged on syringe pump 13, and the speed advancing is set by syringe pump 13.Start syringe pump 13, manage 11 before feed liquid is squeezed into micro pump from syringe 12, by managing 11 arrival spray silk syringe needles 21 before micro pump, due to the effect of surface tension, electric field force, gravity etc., at syringe needle place, form taylor cone.
Electrospinning liquid spinning appts 2 comprises support 22, for driving the reciprocating drive unit 23 of support, a spray silk syringe needle 21 is installed on support, support 22 is connected with drive unit 23.Spray nozzle device also connects spray pressure generation device.Spray nozzle device 3 also comprises spraying syringe 32, control valve 33, the first flexible pipe 34, the second flexible pipe 35, spray nozzle 31 is connected with control valve 33, control valve 33 is connected with spraying syringe 32 by the first flexible pipe 34, in spraying syringe 32, be equipped with and be dissolved with the solution that contains reinforcing material, second flexible pipe 35 one end connect spraying syringe 32, and the other end connects spray pressure generation device 4.The solution of the solution/emulsion that contains supporting material or growth factor is packed in sealable spraying syringe 32, with the first flexible pipe 34, connect syringe 32 and control valve 33, on control valve 33, have flow regulator, can regulate the flow of spraying.32 other end sealings of spraying syringe are also connected with Pressure generator.Pressure generator utilizes the spraying syringe that the common pressurized equipments such as plunger displacement pump, membrane pump give to connect to increase pressure, to can be good at atomization during solution ejection.
Receiving system 5 also comprises rotary electric machine, and rotary electric machine is connected with reception roller.Receiving system 5 is to form by receiving roller 51, bracing frame, shaft coupling and rotary electric machine.Reception roller is fixed on bracing frame by bearing.Connect shaft coupling and be connected and fixed with rotary electric machine, by driven by motor, being rotated.
Embodiment 2
As shown in Figure 2, the difference of the present embodiment and embodiment 1 is: the angle between spray nozzle 31 and spray silk syringe needle 21 is 180 °.This electrospinning liquid spinning appts 2 can be placed on receiving system 5 directly over, also can be placed on the both sides of receiving system 5.The present embodiment, electrospinning liquid spinning appts 2 is positioned over the left side of receiving system 5, and this spinning appts 2 can solderless wrapped connection receiving apparatus 5 90-degree rotations, can, from rotation directly over receiving system 5 to left side, become as the state in Fig. 2.
In the present embodiment, in Fig. 2, spray nozzle device 3 quantity are one, and spray nozzle 31 quantity are also one, still, a plurality of spray nozzle devices 3 can be set, and are convenient to composition abundant and regulation and control spraying ingredient.
All the other structures and the operation principle of the present embodiment are identical with embodiment 1, at this, no longer narrate.
Embodiment 3
Adopt the equipment of embodiment 1, the concrete preparation method that its preparation strengthens electrospun fibers film is as follows.
(1) the electrostatic spinning liquid preparation of electrostatic spinning solution: the PLA stirring and dissolving that is 200,000 by molecular weight, in trifluoroethanol, forms 10%(mass volume ratio).
(2) by above-mentioned dissolving electrostatic spinning liquid completely, pack in syringe, and be arranged in the electrostatic spinning apparatus in embodiment 1.
(3) preparation of spray solution: by the polyvinylpyrrolidone stirring and dissolving of 30k in water, formation 5%(mass volume ratio) solution, in stirring and dissolving, adding fibre diameter is 20 ~ 50 microns, draw ratio is the polypropylene fibre of 1000:1, and the initial strength of this polypropylene fibre is 250 ~ 300MPa.The final mixed solution that forms homogeneous.
(4) above-mentioned mixed solution is packed in spraying syringe, and be arranged in the spray equipment in embodiment 1.
(5) electrostatic spinning parameter: fltting speed 4ml/h; Positive high voltage: 25kv, negative high voltage :-3kv; Receiving range is 21cm.
Spray parameters: feeding speed is 1ml/h, spray distance is 8cm.
(6) open electrostatic spinning apparatus and receiving system, after processing 4h, stop, forming simple acid fiber by polylactic film.
(7) open electrostatic spinning apparatus and receiving system, after processing 0.5h, then open spray equipment.After reprocessing 3.5h, stop.Form the acid fiber by polylactic film of compound PP fiber.
(8), by the tunica fibrosa obtaining in (6) and (7), in the air dry oven of 45 ℃, process 2 days.Then in 50% alcoholic solution, soak 12h, remove residual solvent, finally in the air dry oven of 40 ℃, dry one day.
(9) tunica fibrosa (8) being obtained is cut into the batten of 6cm*1cm, and carries out tensile test.
Pure acid fiber by polylactic film-strength: 4.0 ~ 6.0MPa
The acid fiber by polylactic film-strength of compound PP fiber: 50 ~ 60MPa.
Embodiment 4
Adopt the equipment of embodiment 2, the concrete preparation method that its preparation has enhancing electrospun fibers film is as follows.
(1) the electrostatic spinning liquid preparation of electrostatic spinning solution: the PLGA stirring and dissolving that is 400,000 by molecular weight, in hexafluoroisopropanol, forms 6%(mass volume ratio).
(2) by above-mentioned dissolving electrostatic spinning liquid completely, pack in syringe, and be arranged in the electrostatic spinning apparatus in embodiment 2.
(3) preparation of spray solution: the polyvinyl acetate that is 100,000 by molecular weight and sodium orthophosphate stirring and dissolving, in water, form the solution of the sodium orthophosphate (mass volume ratio) of 5% polyvinyl acetate and 1%, finally form the mixed solution of homogeneous.
(4) above-mentioned mixed solution is packed in spraying syringe, and be arranged in the spray equipment in embodiment 2.
(5) electrostatic spinning parameter: fltting speed 3.5ml/h; Positive high voltage: 28kv, negative high voltage: 0kv; Receiving range is 19cm.Spray parameters:
Feeding speed is 0.8ml/h, and spray distance is 4cm.
(6) open electrostatic spinning apparatus and receiving system, after processing 6h, stop, forming simple PLGA tunica fibrosa.
(7) open electrostatic spinning apparatus and receiving system, after processing 0.5h, then open spray equipment.After reprocessing 5h, stop.Form compound PLGA tunica fibrosa.
(8), by the tunica fibrosa obtaining in (6) and (7), in the air dry oven of 40 ℃, process 4 days.Then in 95% alcoholic solution, soak 8h, remove residual solvent, finally in the air dry oven of 35 ℃, dry two days.
(9) tunica fibrosa (8) being obtained is cut into the batten of 6cm*1cm, and carries out tensile test.
Pure acid fiber by polylactic film-strength: 3.5 ~ 5.0MPa
The acid fiber by polylactic film-strength of compound PP fiber: 15 ~ 20MPa.
Embodiment 5
Adopt the equipment of embodiment 1, its concrete preparation is as follows containing the preparation method of the electrospun fibers film of growth factor.
(1) the electrostatic spinning liquid preparation of electrostatic spinning solution: the PLA stirring and dissolving that is 200,000 by molecular weight, in hexafluoroisopropanol, forms 9%(mass volume ratio).
(2) by above-mentioned dissolving electrostatic spinning liquid completely, pack in syringe, and be arranged in the electrostatic spinning apparatus in embodiment 2.
(3) preparation of spray solution: stem cell factor powder mixing gelatin is dissolved in water, and the ratio of stem cell factor powder and gelatin is 5:2, forms the stem cell factor solution that concentration is 1.5% after dissolving.
(4) above-mentioned solution is packed in spraying syringe, and be arranged in the spray equipment in embodiment 2.
(5) electrostatic spinning parameter: fltting speed 4ml/h; Positive high voltage: 20kv, negative high voltage :-2kv; Receiving range is 21cm.
Spray parameters: feeding speed is 1.5 ml/h, spray distance is 2cm.
(6) open electrostatic spinning apparatus and receiving system, after processing 0.5h, then open spray equipment.After reprocessing 5h, stop spraying, after 0.5h, stop electrostatic spinning.The compound acid fiber by polylactic film that formation contains active stem cell factor.
(7) in 50% alcoholic solution, soak 8h, remove residual solvent, with water for injection, rinse, remove residual alcohol, be finally placed in superclean bench normal temperature air-dry.
(8) cutting, packing, sterilizing.
Obviously, above-described embodiment of the present utility model is only for the utility model example is clearly described, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without also giving all embodiments.All any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in the protection domain of the utility model claim.
Claims (9)
1. a production equipment for electrospun fibers film, comprises electrospinning liquid liquid feed device (1), the electrospinning liquid spinning appts (2) being connected with electrospinning liquid liquid feed device (1), and electrospinning liquid spinning appts (2) is provided with spray silk syringe needle (21); Also comprise receiving system (5) and the first HV generator (6), the second HV generator (7), receiving system (5) is provided with the reception roller (51) of rotatable reception spray silk syringe needle (21) ejection fiber, the first HV generator (6) is electrically connected to spray silk syringe needle (21), and the second HV generator (7) is electrically connected to reception roller (51);
It is characterized in that, also comprise spray nozzle device (3), described spray nozzle device (3) is provided with spray nozzle (31), spray nozzle (31) and the angled setting of spray silk syringe needle (21).
2. the production equipment of electrospun fibers film according to claim 1, it is characterized in that: described spray nozzle (31) is all located at and is received roller (51) periphery with spray silk syringe needle (21), the angle between spray nozzle (31) and spray silk syringe needle (21) is 90 °～180 °.
3. the production equipment of electrospun fibers film according to claim 1, it is characterized in that: described electrospinning liquid liquid feed device (1) is managed (11), syringe (12), syringe pump (13) before comprising micro pump, before micro pump, pipe (11) one end connects spray silk syringe needle (21), the other end connects syringe (12), and syringe (12) connects syringe pump (13).
4. the production equipment of electrospun fibers film according to claim 1, it is characterized in that: described electrospinning liquid spinning appts (2) comprises support (22), for driving the reciprocating drive unit of support (22) (23), it is upper that spray silk syringe needle (21) is installed on support (22), and support (22) is connected with drive unit (23).
5. the production equipment of electrospun fibers film according to claim 2, is characterized in that: described spray nozzle device (3) also connects spray pressure generation device (4).
6. the production equipment of electrospun fibers film according to claim 5, it is characterized in that: described spray nozzle device (3) also comprises spraying syringe (32), control valve (33), the first flexible pipe (34), the second flexible pipe (35), spray nozzle (31) is connected with control valve (33), control valve (33) is connected with spraying syringe (32) by the first flexible pipe (34), spraying syringe (32) has the space of loading spray coating liquor, the second flexible pipe (35) one end connects spraying syringe (32), and the other end connects spray pressure generation device (4).
7. according to the production equipment of the arbitrary described electrospun fibers film of claim 1 to 6, it is characterized in that: described receiving system (5) also comprises rotary electric machine, rotary electric machine is connected with reception roller (51).
8. according to the production equipment of the arbitrary described electrospun fibers film of claim 1 to 6, it is characterized in that: the distance of described spray nozzle (31) and reception roller (51) is for being less than or equal to 100mm.
9. the production equipment of electrospun fibers film according to claim 8, is characterized in that: described spray nozzle (31) is 2 ~ 100mm with the distance that receives roller (51).
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| CN201420143200.8U CN203821054U (en) | 2014-03-27 | 2014-03-27 | Production equipment for electrostatic spinning fibrous membrane |
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| CN103911767A (en) * | 2014-03-27 | 2014-07-09 | 广州迈普再生医学科技有限公司 | Electrospinning fibrous membrane production device and method of producing electrospinning fibrous membranes thereby |
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- 2014-03-27 CN CN201420143200.8U patent/CN203821054U/en not_active Expired - Lifetime
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103911767A (en) * | 2014-03-27 | 2014-07-09 | 广州迈普再生医学科技有限公司 | Electrospinning fibrous membrane production device and method of producing electrospinning fibrous membranes thereby |
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| CN106868712A (en) * | 2015-12-14 | 2017-06-20 | 中国科学院过程工程研究所 | A kind of Membrane Materials nano fibrous membrane and preparation method thereof |
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| CN113244448A (en) * | 2021-05-06 | 2021-08-13 | 东华大学 | Abdominal wall tissue repair patch and preparation method thereof |
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