CN203788985U - Liquid nitrogen frozen device for body tissue - Google Patents

Liquid nitrogen frozen device for body tissue Download PDF

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Publication number
CN203788985U
CN203788985U CN201420062080.9U CN201420062080U CN203788985U CN 203788985 U CN203788985 U CN 203788985U CN 201420062080 U CN201420062080 U CN 201420062080U CN 203788985 U CN203788985 U CN 203788985U
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CN
China
Prior art keywords
fan
shaped
column
cover
handle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201420062080.9U
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Chinese (zh)
Inventor
张立德
苗嘉芮
曲怡
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Liaoning University of Traditional Chinese Medicine
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Liaoning University of Traditional Chinese Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Liaoning University of Traditional Chinese Medicine filed Critical Liaoning University of Traditional Chinese Medicine
Priority to CN201420062080.9U priority Critical patent/CN203788985U/en
Application granted granted Critical
Publication of CN203788985U publication Critical patent/CN203788985U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses a liquid nitrogen frozen device for a body tissue, which comprises a round external cylinder, an upper cover of the round external cylinder and a round internal cylinder, wherein the upper cover of the round external cylinder is buckled on the upper end opening of the round external cylinder; the side wall of the round external cylinder is fixedly provided with a handle; the round internal cylinder is spliced by six fan-shaped columns with the same cross sections; three side walls of each column are grid-shaped walls; the upper end opening of each column is hinged with a fan-shaped upper cover which is fixedly provided with an upper grip and an upper handle and can tightly seal the upper end opening of the column; the upper grip can lift the fan-shaped upper cover to a 90-degree position to be opened; the lower end opening of each column is hinged with a fan-shaped lower cover which is fixedly provided with a lower grip and can tightly seal the lower end opening of the column; the lower grip can lift the fan-shaped lower cover to a 90-degree position to be opened. The liquid nitrogen frozen device disclosed by the utility model is convenient to use, the body tissue for en experiment is good in freezing effect and liquid nitrogen is saved.

Description

A kind of body tissue liquid nitrogen frozen device
Technical field
The utility model relates to a kind of laboratory instant freezer soma apparatus, specifically a kind of by each tissue typing of body, and carries out freezing liquid nitrogen frozen device.
Background technology
Under atmospheric pressure, liquid nitrogen is converted into gaseous state by liquid state, and the evaporation latent heat that average per kilogram need to absorb is 199.29 kilojoules, then the heat absorbing while rising to 273K by 77.35K is 202 kilojoules.It is exactly the principle of utilizing ultralow temperature heat absorption that liquid nitrogen makes tissue or albumen quick-frozen, when draw materials in laboratory, the EP pipe that biomaterial is housed is directly immersed in liquid nitrogen, carry out strong heat exchange, instantaneous the freezing of 20min left and right biomaterial, generally just can complete freeze all process, fast 30 times than common freezing.Because the time of freezing is short, the ice crystal of generation is just very tiny, and freezing speed is greater than the inside and outside steam seepage velocity of cell, produces ice crystal inside and outside cell simultaneously, and to cell, without destruction, therefore, the artifact material that thaws still can normally be used.
Although liquid nitrogen is good compared with refrigerating effect, easily evaporation, existing ice bag, is placed on the tissue in EP pipe on ice bag during use, but this method refrigerating effect is poor, easily makes the degraded of tissue or albumen corrupt.
Utility model content
The purpose of this utility model, is to provide a kind of body tissue liquid nitrogen frozen device for the problems referred to above, solves the current ice bag freezing tissue of using, the problem of the bad and trouble of refrigerating effect.
The technical scheme adopting is:
A body tissue liquid nitrogen frozen device, comprises outer ring cylindrical shell, outer ring cylinder top cover and interior circular cylinder body, and outer ring cylinder top cover be located in the upper port place of outer ring cylindrical shell, on the sidewall of outer ring cylindrical shell, is fixed with handle, it is characterized in that:
Interior circular cylinder body is that fan-shaped column amalgamation forms by six identical cross sections of structure.
Three sidewalls of column are mesh walls, and the upper port place of column is hinged with fan-shaped upper cover, cover on fan-shaped and are fixed with respectively handle and upper handle, and fan-shaped upper cover can tightly seal the upper port of column.Upper handle can upwards be mentioned fan-shaped upper cover to 90 degree and be opened.The lower port place of column is hinged with fan-shaped lower cover, covers under fan-shaped and is fixed with lower handle, and fan-shaped lower cover can tightly seal the lower port of column, and lower handle can lift fan-shaped lower cover downwards and become 90 degree to open.
During use, experimenter surpasses 2/3 of cylinder by putting into liquid nitrogen in outer ring cylindrical shell, in experiment, open fan-shaped upper cover, put one into containing covering fan-shaped upper cover after tissue or protein EP-2 pipe at once, operation so repeatedly while drawing materials, fan-shaped upper cover seals up the upper port of column, prevent liquid nitrogen volatilization, after experiment finishes, respectively each column is proposed by upper handle, by lower handle, open fan-shaped lower cover, the EP pipe that tissue or albumen are housed is poured in the bag of having classified, then by upper handle, liquid nitrogen is refunded, economize on resources and avoid waste, finally buckling outer ring cylinder top cover prevents from polluting.
Adopt the good effect of technique scheme: take the fan-shaped column as cross section for 6 and open handled fan-shaped upper cover when experiment, cover upper cover after putting a tissue or albumen into, can reduce the evaporation of liquid nitrogen at once; Cylinder is divided into 6 lattice to tissue is classified; When the net wall of column side can guarantee tissue or albumen to mention, without liquid nitrogen, and experiment is while finishing pouring liquid, and liquid nitrogen becomes a post, rather than from each lattice, flows out respectively, and this scheme makes the refrigerating effect of drawing materials better, and method is more convenient.Covering outer ring cylinder top cover can prevent from polluting.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is that cross section is fan-shaped column structural representation.
Embodiment
A body tissue liquid nitrogen frozen device, comprises outer ring cylindrical shell 1, outer ring cylinder top cover 2 and interior circular cylinder body, and outer ring cylinder top cover 2 be located in the upper port place of outer ring cylindrical shell 1, is fixed with handle 4 on the sidewall of outer ring cylindrical shell 1, it is characterized in that:
Interior circular cylinder body is that fan-shaped column 5 amalgamations form by six identical cross sections of structure.
Three sidewalls of column 5 are mesh walls 10, and the upper port place of column 5 is hinged with fan-shaped upper cover 6, are fixed with respectively handle 7 and upper handle 3 on fan-shaped upper cover 6, and fan-shaped upper cover 6 can tightly seal the upper port 8 of column 5.Upper handle 7 can upwards be mentioned fan-shaped upper cover 6 to 90 degree and be opened.The lower port place of column 5 is hinged with fan-shaped lower cover 9, is fixed with lower handle 11 on fan-shaped lower cover 9, and fan-shaped lower cover 9 can tightly seal the lower port of column 5, and lower handle 11 can lift 9 one-tenth 90 degree of fan-shaped lower cover downwards and open.

Claims (1)

1. a body tissue liquid nitrogen frozen device, comprise outer ring cylindrical shell (1), outer ring cylinder top cover (2) and interior circular cylinder body, outer ring cylinder top cover (2) be located in the upper port place of outer ring cylindrical shell (1), on the sidewall of outer ring cylindrical shell (1), be fixed with handle (4), it is characterized in that:
Interior circular cylinder body is that fan-shaped column (5) amalgamation forms by six identical cross sections of structure;
Three sidewalls of column (5) are mesh walls (10), the upper port place of column (5) is hinged with fan-shaped upper cover (6), on fan-shaped upper cover (6), be fixed with respectively handle (7) and upper handle (3), fan-shaped upper cover (6) can tightly seal the upper port (8) of column (5); Upper handle (7) can upwards be mentioned fan-shaped upper cover (6) to 90 degree and be opened; The lower port place of column (5) is hinged with fan-shaped lower cover (9), on fan-shaped lower cover (9), be fixed with lower handle (11), fan-shaped lower cover (9) can tightly seal the lower port of column (5), and lower handle (11) can lift fan-shaped lower cover (9) downwards and become 90 degree to open.
CN201420062080.9U 2014-02-12 2014-02-12 Liquid nitrogen frozen device for body tissue Expired - Fee Related CN203788985U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420062080.9U CN203788985U (en) 2014-02-12 2014-02-12 Liquid nitrogen frozen device for body tissue

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420062080.9U CN203788985U (en) 2014-02-12 2014-02-12 Liquid nitrogen frozen device for body tissue

Publications (1)

Publication Number Publication Date
CN203788985U true CN203788985U (en) 2014-08-27

Family

ID=51371284

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201420062080.9U Expired - Fee Related CN203788985U (en) 2014-02-12 2014-02-12 Liquid nitrogen frozen device for body tissue

Country Status (1)

Country Link
CN (1) CN203788985U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108514067A (en) * 2018-03-29 2018-09-11 中国农业科学院蜜蜂研究所 A kind of cryopreservation device and its cryopreservation methods for royal jelly

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108514067A (en) * 2018-03-29 2018-09-11 中国农业科学院蜜蜂研究所 A kind of cryopreservation device and its cryopreservation methods for royal jelly

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Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140827

Termination date: 20170212

CF01 Termination of patent right due to non-payment of annual fee