CN203768359U - Minimally invasive smearing type crop seedling inoculating machine - Google Patents

Minimally invasive smearing type crop seedling inoculating machine Download PDF

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Publication number
CN203768359U
CN203768359U CN201420187454.XU CN201420187454U CN203768359U CN 203768359 U CN203768359 U CN 203768359U CN 201420187454 U CN201420187454 U CN 201420187454U CN 203768359 U CN203768359 U CN 203768359U
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China
Prior art keywords
wicresoft
cylinder
inoculating
liquid
fixed
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CN201420187454.XU
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Chinese (zh)
Inventor
苑进
刘雪美
胡敏
李现道
苏建东
杨举田
谭效磊
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Shandong Agricultural University
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Shandong Agricultural University
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Abstract

The utility model relates to a minimally invasive smearing type crop seedling inoculating machine. The technical scheme adopted by the utility model is as follows: the machine comprises a mechanical bracket, an inoculating device, a traveling device and an air flow assistant device, wherein the mechanical bracket is used for fixing and supporting other devices and adjusting the height of the inoculating device, the inoculating device is used for inoculating antiviral vaccines of crop seedlings in an inoculating area, the traveling device is used for driving the mechanical bracket to travel on the crop inoculating area at a certain speed, and the air flow assistant device is used for generating an assistant air flow in the inoculating process to blow the crop seedling to the inoculating device. Aiming at the problem of intensive vaccine inoculation of the crop seedlings, the inoculating machine provided by the utility model blows the crop seedlings to a minimally invasive roller by adopting the assistant air flow, rotates the minimally invasive crop spires through the roller with waterproof sand paper and smear an inoculating liquid medicine through thin sponge, so that accurate inoculation is realized and the inoculating rate is improved. The inoculating machine can be suitable for inoculating crop seedlings in different growths to realize intensive inoculation of greenhouse crop seedlings.

Description

A kind of Wicresoft painting type crop seedling inoculation device
Technical field
The utility model relates to equipment for plant protection technical field, is a kind of Wicresoft's painting type crop seedling inoculation device for nursery stage crop, relates in particular to the inoculation of greenhouse seedling being carried out to low virus vaccine.
Technical background
The viroses of plant are that current proportion of crop planting district distributes extensively, occurs a comparatively general large class disease, and it is one of important restriction factor of farm crop production that virus disease has become, and huge financial loss is caused in plantation family.Excessive use agricultural chemicals can not effectively be controlled the generation of this type of disease of crop, increases on the contrary the pesticide residue of crop.Virus inoculation vaccine is considered to effective substitute of agrochemical agricultural chemicals from now on.
Crop virus vaccines inoculation at present adopts rubbing manipulation inoculation, but owing to lacking simple inoculating mechanism efficiently, adopts artificial inoculation operation more, and the heavy poor efficiency of workload, cannot realize the intensive development of crop seedling inoculation mass-producing.Therefore in the urgent need to inventing a kind of high-efficiency crop virus vaccines inoculating mechanism, carry out mechanize inoculation, for guaranteeing crop yield, reduce the quality of using, lowering farm crop pesticide residue, improve crop of chemical agent most important.
The manual work poor efficiency, unstable, the domestic inoculating mechanism of growing seedlings of rate of vaccination that for farm crop inoculation, exist are still located blank, cannot realize the problems such as mechanize work of virus vaccines inoculation, invent the crop inoculation device of a kind of self-propelled, high-level efficiency and high rate of vaccination, to complete the high-efficiency inoculating work of crop seedling phase.Under the requirement that is meeting crop seedling high-efficiency low-damage inoculation, realize the development of crop inoculation mass-producing, for the development of plant protection mechanism, there is pushing effect.
Retrieval through prior art document is found, there is no the relevant report of crop inoculating mechanism, relates in particular to a kind of Wicresoft painting type crop seedling inoculating mechanism and belongs to pioneering.
Summary of the invention
A kind of Wicresoft painting type crop seedling inoculation device that the utility model is invented in order to solve the intensive vaccine inoculation problem of crop seedling, realizes the work of greenhouse seedling mass-producing preventing disease and pest.
The utility model relates to the technical scheme that a kind of Wicresoft painting type crop seedling inoculation device adopts: comprise machinery mount, classification inoculation apparatus, running gear, air-flow supplementary unit.Described machinery mount is for height fixing and that support other devices and adjust classification inoculation apparatus; Described classification inoculation apparatus is for inoculating the inoculation of the antiviral vaccine of district's crop seedling; Described running gear is walked with certain speed for driving device support in crop inoculation district; Described air-flow supplementary unit produces substreams for seeded process, and crop seedling is blowed to classification inoculation apparatus.
Described machinery mount comprises frame and height control frame.Described frame is the framework that is connected and is formed by rod member.Described height control frame comprises set screw, fixed outer cylinder and elevating bracket; Described fixed outer cylinder is fastened on the symmetric position of frame lower both sides, and elevating bracket is mobilizable to be socketed in fixed outer cylinder, and elevating bracket can fixedly move up and down in inner core, and the screw that is locked is for the fixing proper height of elevating bracket.
Described classification inoculation apparatus comprises mechanism of Wicresoft and liquid transfer mechanism.Described mechanism of Wicresoft comprises Wicresoft's cylinder and driving mechanism.Mechanism of Wicresoft is fixed on elevating bracket, changes the height that the relative position of elevating bracket in fixed outer cylinder can regulate mechanism of Wicresoft.By one section of two ends, the straight tube with rotating shaft forms with one deck water-proof abrasive paper that covers thin sponge outside surface with the thin sponge of one deck that covers straight tube surface described Wicresoft's cylinder; The staggered hollow hole that is provided with of water-proof abrasive paper.The thin spongy layer that openwork hole exposes and water-proof abrasive paper form and are interspersed at Wicresoft's cylinder surface.Described driving mechanism is comprised of buncher, U-shaped support, bearing support, driving gear, revolving gear.The two ends rotating shaft of Wicresoft's cylinder is fixed on elevating bracket by bearing support, and revolving gear is connected in the rotating shaft of Wicresoft's cylinder; Described buncher is fixed on elevating bracket by U-shaped support, and driving gear is fixed on buncher output shaft by key, driving gear and revolving gear engagement, and buncher drives Wicresoft's cylinder to rotate by geartransmission.
Described liquid transfer mechanism comprises virus vaccines fluid storage tank, top liquid pipe, pipeline connection, pressure pump, flowrate control valve, bottom liquid pipe and virus vaccines distribution pipe.Described virus vaccines fluid storage tank is fixed on frame top, and virus vaccines fluid storage tank is used for preserving inoculation liquid; Described top liquid pipe is communicated with virus vaccines fluid storage tank and pressure pump; Described pressure pump is fixed on frame top, is connected with flowrate control valve on pressure pump, and pressure pump is for being transported to flowrate control valve by the liquid of fluid storage tank; Described bottom liquid pipe is communicated with flowrate control valve and virus vaccines distribution pipe, for liquid is further delivered to virus vaccines distribution pipe from flowrate control valve; Described virus vaccines distribution pipe is comprised of liquid distributor, cloth liquid sponge and circular arc fixed plate.Described liquid distributor is one section of hollow straight tube, and hollow straight tube top is fixed with reclinate circular arc fixed plate; Below circular arc fixed plate and liquid distributor, be provided with cloth liquid sponge; Liquid distributor be positioned at Wicresoft's cylinder directly over Qie Yu Wicresoft cylinder be arranged in parallel; The diameter of circular arc fixed plate is greater than the diameter of liquid distributor and Wicresoft's cylinder; Liquid distributor lower surface has row's roundlet type through hole along Wicresoft's drum length direction directly over Wicresoft's cylinder, be positioned at spacing maximum between two symmetrical circular holes of liquid distributor center, spacing diminishes to two ends gradually, virus vaccines distribution pipe is identical with Wicresoft drum length, and liquid distributor two ends are fixed on U-shaped support top; Described cloth liquid sponge is one deck and liquid distributor sponge equal in length, and cloth liquid sponge is connected with Wicresoft cylinder contact, and Bing Yu Wicresoft cylinder forms circular arc type contact surface.When Wicresoft's cylinder rotates, virus vaccines distribution pipe is uniformly distributed inoculation liquid by cloth liquid sponge, spread upon on the thin sponge and water-proof abrasive paper of Wicresoft's cylinder surface.
Described running gear is comprised of travel wheel, drive-motor, sprocket wheel, chain travel wheel support and horizontal rail.Travel wheel is fixed on frame bottom by travel wheel support, and travel wheel is placed in horizontal rail.Described drive-motor is fixed on the side of frame, and drive-motor and travel wheel adopt chain drive, and drive chassis is walked along horizontal rail.Described horizontal rail fixes on the ground, and its length should equal to inoculate the length in district.
Described air-flow supplementary unit is comprised of blower fan, fan housing, flow-guiding mouth.Described blower fan is fixed in frame, and fan outlet, fan housing and flow-guiding mouth are connected successively.Described fan housing selects the soft materials such as waterproof cloth to make.Described flow-guiding mouth and Wicresoft's cylinder horizontal alignment, Bing Yu Wicresoft cylinder keeps certain spacing, and preferably, flow-guiding mouth and Wicresoft's cylinder keep the spacing of 15cm~25cm; Flow-guiding mouth is fixed on elevating bracket by the support at its two ends.The air-flow that blower fan blows out, successively by fan housing and flow-guiding mouth, blows to mechanism of Wicresoft by crop seedling.
An inoculation method for Wicresoft's painting type crop seedling inoculation device, comprises the steps:
1) prepare inoculation liquid: vaccine inoculation liquid and water are sneaked into according to the specified ratio to virus vaccines fluid storage tank and stirred, adjust the pressure of pressure pump, adjustment flow valve on-off control medicinal liquid flow is carried out inoculation and prepared.
2) adjust classification inoculation apparatus height: for the growing way of growing seedlings in inoculation region, regulate the height of mechanism of Wicresoft, make Wicresoft's cylinder can fully touch crop spire.
3) adjust inoculation device gait of march: the gait of march that inoculation device needs is set, inoculation device is reset to horizontal rail starting point, by running gear control section, adjust test set along the gait of march in horizontal rail, assurance inoculation device is realized and at the uniform velocity being advanced in inoculation region.
4) inoculation: enter before inoculation region, trigger pressure pump, the thin sponge of Wicresoft's cylinder is full of liquid, and Wicresoft's cylinder reaches stable rotation, now makes inoculation at the uniform velocity by inoculation region.Complete after a line, next inoculation district's operation is carried out in line feed.
The utlity model has following advantage:
1) the utility model adopts the cylinder with water-proof abrasive paper running to carry out Wicresoft to crop spire, and substreams part in addition obtains simultaneously not damaging seedling, improves rate of vaccination.
2) height of the utility model capable of regulating classification inoculation apparatus, can adapt to the crop seedling inoculation of different growing ways, realizes chamber crop seedling and can intensively inoculate.
3) after the utility model adopts cylinder Wicresoft, thin sponge is smeared spire inoculation liquid, and precisely inoculation, reduces liquor residue.
4) the utility model is simple to operate, cost is low, efficiency is high, is adapted at field large-scale inoculation virus, can be used to inoculate various low virus vaccines, to protect plant to avoid strong virus, infects.
Accompanying drawing explanation
Fig. 1 is the structural representation of virus vaccines painting type crop seedling inoculation device of the present utility model;
Fig. 2 is inoculation device front view;
Fig. 3 is the left view of inoculation device;
Fig. 4 is the Local map of inoculation device inside;
Mechanism of Tu5Shi Wicresoft and virus vaccines liquid distributor structure iron;
Fig. 6 is the sectional view of the A-A of Fig. 5;
Fig. 7 is the sectional view of the B-B of Fig. 5;
The layout of Tu8Shi Wicresoft cylinder surface sponge and sand paper;
In figure: 1, horizontal rail 2, travel wheel 3, frame 4, pressure pump 5, virus vaccines fluid storage tank 6, gear hurdle 7, fan housing 8, drive-motor, 9, blower fan 10, flowrate control valve 11, top liquid pipe, 12, travel wheel support 13, flow-guiding mouth 14, bottom liquid pipe 15, fan fixed mount 16, chain 17, sprocket wheel 18, set screw 19, buncher 20, circular arc fixed plate 21, U-shaped support 22, elevating bracket 23, fixed outer cylinder 24, driving gear 25, liquid distributor 26, revolving gear 27, bearing support, 28, water-proof abrasive paper 29, thin sponge 30, straight tube 31, cloth liquid sponge
Embodiment:
Below in conjunction with accompanying drawing, the utility model patent is described further.A kind of Wicresoft described in the utility model painting type crop seedling inoculation device, as shown in Fig. 1,2,3,4,5, described machinery mount comprises frame (3), height control frame.Described frame (3) is the framework that is connected and is formed by rod member.Described height control frame is comprised of set screw (18), fixed outer cylinder (23) and elevating bracket (22); Described fixed outer cylinder (23) is fastened on the symmetrical position of frame (3) bottom, and elevating bracket (22) is socketed in fixed outer cylinder (23), and the screw that is locked (18) is for the fixing height of elevating bracket (22).
As shown in Fig. 1,2,5,6,7,8, described classification inoculation apparatus comprises mechanism of Wicresoft, liquid transfer mechanism.Described mechanism of Wicresoft comprises by Wicresoft's cylinder and driving mechanism.It is upper that mechanism of Wicresoft is fixed in elevating bracket (22), changes elevating bracket and can regulate the height of mechanism of Wicresoft at the relative position of fixed outer cylinder (23).By one section of two ends, the straight tube with rotating shaft forms with one deck water-proof abrasive paper that covers thin sponge outside surface with the thin sponge of one deck that covers straight tube surface described Wicresoft's cylinder; The staggered hollow hole that is provided with of water-proof abrasive paper (28), the thin spongy layer that hollow part exposes and water-proof abrasive paper form and are interspersed at Wicresoft's cylinder surface.For example, preferably, the water-proof abrasive paper covering is provided with the rectangular opening (as shown in Figure 8) of the 3cm * 3cm of a hollow out at interval of the rectangle of 3cm * 3cm, in drum rotating Wicresoft of Wicresoft inoculation seedling process, form the alternating action that water-proof abrasive paper Wicresoft seedling and thin sponge are smeared inoculation liquid.Described driving mechanism comprises buncher (19), U-shaped support (21), bearing support (27), driving gear (24) and revolving gear (26).The two ends rotating shaft of Wicresoft's cylinder is fixed on elevating bracket by bearing support (27), and revolving gear (26) is connected in the rotating shaft of Wicresoft's cylinder; Described buncher (19) is fixed on elevating bracket (22) by U-shaped support (21), driving gear (24) is fixed on buncher output shaft by key, driving gear (24) and revolving gear (26) engagement, buncher (19) drives Wicresoft's cylinder to rotate by geartransmission.Described liquid transfer mechanism comprises virus vaccines fluid storage tank (5), top liquid pipe (11), pipeline connection, pressure pump (4), flowrate control valve (10), bottom liquid pipe (14) and virus vaccines distribution pipe.Described virus vaccines fluid storage tank (5) is fixed on frame top, for preserving inoculation liquid; Described top liquid pipe (11) is communicated with virus vaccines fluid storage tank (5) and pressure pump (4); Described pressure pump (4) and flowrate control valve (10) are fixed on frame (3) top, and pressure pump is connected with flowrate control valve (10), and pressure pump is for being transported to flowrate control valve (10) by the liquid of virus vaccines fluid storage tank (5); Described bottom liquid pipe (14) is communicated with flowrate control valve and virus vaccines distribution pipe, for liquid is further delivered to virus vaccines distribution pipe from flowrate control valve; Described virus vaccines distribution pipe is comprised of liquid distributor (25), cloth liquid sponge (31) and circular arc fixed plate (20).Described liquid distributor (25) is one section of hollow straight tube, and hollow straight tube top is fixed with reclinate circular arc fixed plate (20); Liquid distributor (25) be positioned at Wicresoft's cylinder directly over Qie Yu Wicresoft cylinder be arranged in parallel; Below circular arc fixed plate and liquid distributor, be provided with cloth liquid sponge; The diameter of circular arc fixed plate (20) is greater than the diameter of liquid distributor and Wicresoft's cylinder; Hollow straight tube has a row roundlet type through hole along Wicresoft's drum length direction at lower surface directly over Wicresoft's cylinder, be positioned at spacing maximum between two symmetrical circular holes of liquid distributor center, spacing diminishes to two ends gradually, virus vaccines distribution pipe is identical with Wicresoft drum length, and liquid distributor two ends are fixed on U-shaped support top; Described cloth liquid sponge (31) is one deck and liquid distributor sponge equal in length, is connected with Wicresoft cylinder contact, and Bing Yu Wicresoft cylinder forms circular arc type contact surface.When Wicresoft's cylinder rotates, virus vaccines distribution pipe is uniformly distributed inoculation liquid by cloth liquid sponge, spread upon on the thin sponge (29) and water-proof abrasive paper (28) of Wicresoft's cylinder surface.
As shown in Figure 1,3, described running gear is comprised of travel wheel (2), drive-motor (8), sprocket wheel (17), chain (16), travel wheel support (12) and horizontal rail (1).Travel wheel (2) is fixed on frame bottom by travel wheel support (12), and travel wheel (2) is placed in horizontal rail (1).Described drive-motor (8) is fixed on the side of frame, and drive-motor and travel wheel adopt chain drive, and drive chassis is walked along horizontal rail.Described horizontal rail fixes on the ground, and its length should equal to inoculate the length in district;
Air-flow supplementary unit as described in Fig. 1,2,3,4 is comprised of blower fan (9), fan housing (7), flow-guiding mouth (13).The air outlet of described blower fan (9) is connected with flow-guiding mouth (13) with fan housing (7) successively.Described fan housing (7) selects the soft materials such as waterproof cloth to make.Described flow-guiding mouth (13) and mechanism of Wicresoft horizontal alignment, Bing Yu Wicresoft cylinder keeps certain spacing, and preferably, flow-guiding mouth and mechanism of Wicresoft keep the spacing of 15cm~25cm; Flow-guiding mouth (13) two ends are fixed on elevating bracket by the support at two ends.The air-flow that blower fan blows out, successively by fan housing and flow-guiding mouth, blows to mechanism of Wicresoft by crop seedling.
An inoculation method for Wicresoft's painting type crop seedling inoculation device, comprises the steps:
1) prepare inoculation liquid: vaccine inoculation liquid and water are sneaked into according to the specified ratio to virus vaccines fluid storage tank (5) and stirred, adjust the pressure of pressure pump (4), adjustment flowrate control valve (10) on-off control medicinal liquid flow is carried out inoculation and prepared.
2) adjust classification inoculation apparatus height: for the growing way of growing seedlings in inoculation region, regulate the height of mechanism of Wicresoft, make Wicresoft's cylinder can fully touch crop spire.
3) adjust inoculation device gait of march: the gait of march that inoculation device needs is set, inoculation device is reset to horizontal rail starting point, by running gear control section, adjust test set along the gait of march in horizontal rail, assurance inoculation device is realized and at the uniform velocity being advanced in inoculation region.
4) inoculation: enter before inoculation region, trigger pressure pump (4), the thin sponge of Wicresoft's cylinder is full of liquid, and Wicresoft's cylinder reaches stable rotation, now makes inoculation at the uniform velocity by inoculation region.Complete after a line, next inoculation district's operation is carried out in line feed.

Claims (1)

1.Yi Zhong Wicresoft painting type crop seedling inoculation device, is characterized in that comprising machinery mount, classification inoculation apparatus, running gear, air-flow supplementary unit;
Described machinery mount comprises frame and height control frame; Described frame is the framework that is connected and is formed by rod member; Described height control frame comprises set screw, fixed outer cylinder and elevating bracket; Described fixed outer cylinder is fastened on the symmetric position of frame lower both sides, and elevating bracket is mobilizable to be socketed in fixed outer cylinder, and elevating bracket fixedly can move up and down in inner core, and the screw that is locked is for the fixing height of elevating bracket;
Described classification inoculation apparatus comprises mechanism of Wicresoft and liquid transfer mechanism; Described mechanism of Wicresoft comprises Wicresoft's cylinder and driving mechanism; Mechanism of Wicresoft is fixed on elevating bracket, changes the height that the relative position of elevating bracket in fixed outer cylinder regulates mechanism of Wicresoft; By one section of two ends, the straight tube with rotating shaft forms with one deck water-proof abrasive paper that covers thin sponge outside surface with the thin sponge of one deck that covers straight tube surface described Wicresoft's cylinder; The staggered hollow hole that is provided with of water-proof abrasive paper; The thin spongy layer that openwork hole exposes and water-proof abrasive paper form and are interspersed at Wicresoft's cylinder surface; Described driving mechanism comprises buncher, U-shaped support, bearing support, driving gear and revolving gear; The two ends rotating shaft of Wicresoft's cylinder is fixed on elevating bracket by bearing support, and revolving gear is connected in the rotating shaft of Wicresoft's cylinder; Described buncher is fixed on elevating bracket by U-shaped support, and driving gear is fixed on buncher output shaft by key, driving gear and revolving gear engagement, and buncher drives Wicresoft's cylinder to rotate by geartransmission;
Described liquid transfer mechanism comprises virus vaccines fluid storage tank, top liquid pipe, pipeline connection, pressure pump, flowrate control valve, bottom liquid pipe and virus vaccines distribution pipe; Described virus vaccines fluid storage tank is fixed on frame top, and virus vaccines fluid storage tank is used for preserving inoculation liquid; Described top liquid pipe is communicated with virus vaccines fluid storage tank and pressure pump; Described pressure pump is fixed on frame top, is connected with flowrate control valve on pressure pump; Described bottom liquid pipe is communicated with flowrate control valve and virus vaccines distribution pipe; Described virus vaccines distribution pipe is comprised of liquid distributor, cloth liquid sponge and circular arc fixed plate; Described liquid distributor is one section of hollow straight tube, and hollow straight tube top is fixed with reclinate circular arc fixed plate; Below circular arc fixed plate and liquid distributor, be provided with cloth liquid sponge; Liquid distributor be positioned at Wicresoft's cylinder directly over Qie Yu Wicresoft cylinder be arranged in parallel; The diameter of circular arc fixed plate is greater than the diameter of liquid distributor and Wicresoft's cylinder; Liquid distributor lower surface has row's roundlet type through hole along Wicresoft's drum length direction directly over Wicresoft's cylinder, be positioned at spacing maximum between two symmetrical circular holes of liquid distributor center, spacing diminishes to two ends gradually, virus vaccines distribution pipe is identical with Wicresoft drum length, and liquid distributor two ends are fixed on U-shaped support top; Described cloth liquid sponge is one deck and liquid distributor sponge equal in length, and cloth liquid sponge is connected with Wicresoft cylinder contact, and Bing Yu Wicresoft cylinder forms circular arc type contact surface; When Wicresoft's cylinder rotates, virus vaccines distribution pipe is uniformly distributed inoculation liquid and spreads upon by cloth liquid sponge on the thin sponge and water-proof abrasive paper of Wicresoft's cylinder surface;
Described running gear comprises travel wheel, drive-motor, sprocket wheel, chain and horizontal rail; Travel wheel is fixed on frame bottom by travel wheel support, and travel wheel is placed in horizontal rail; Described drive-motor is fixed on the side of frame, and drive-motor and travel wheel adopt chain drive, and drive chassis is walked along horizontal rail; Described horizontal rail fixes on the ground, and its length equals to inoculate the length in district;
Described air-flow supplementary unit comprises blower fan, fan housing and flow-guiding mouth; Described blower fan is fixed in frame, and fan outlet, fan housing and flow-guiding mouth are connected successively; Described flow-guiding mouth and Wicresoft's cylinder horizontal alignment, Bing Yu Wicresoft cylinder keeps the spacing of 15cm~25cm; Flow-guiding mouth is fixed on elevating bracket by the support at its two ends.
CN201420187454.XU 2014-04-17 2014-04-17 Minimally invasive smearing type crop seedling inoculating machine Withdrawn - After Issue CN203768359U (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103952297A (en) * 2014-04-17 2014-07-30 山东农业大学 Physical minimally-invasive crop seedling inoculator and inoculation method thereof
CN104686259A (en) * 2015-03-25 2015-06-10 山东烟草研究院有限公司 Needling type tobacco seedling inoculation machine and needling type tobacco seedling inoculation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103952297A (en) * 2014-04-17 2014-07-30 山东农业大学 Physical minimally-invasive crop seedling inoculator and inoculation method thereof
CN104686259A (en) * 2015-03-25 2015-06-10 山东烟草研究院有限公司 Needling type tobacco seedling inoculation machine and needling type tobacco seedling inoculation method

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Granted publication date: 20140813

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