CN203759021U - Norovirus detection kit - Google Patents
Norovirus detection kit Download PDFInfo
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- CN203759021U CN203759021U CN201320705300.0U CN201320705300U CN203759021U CN 203759021 U CN203759021 U CN 203759021U CN 201320705300 U CN201320705300 U CN 201320705300U CN 203759021 U CN203759021 U CN 203759021U
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- Prior art keywords
- pad
- norovirus
- sample
- nitrocellulose filter
- filter
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- 241001263478 Norovirus Species 0.000 title claims abstract description 60
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 33
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 33
- 238000006243 chemical reaction Methods 0.000 claims abstract description 19
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002250 absorbent Substances 0.000 claims description 18
- 230000002745 absorbent Effects 0.000 claims description 18
- 238000012360 testing method Methods 0.000 claims description 17
- 108090000565 Capsid Proteins Proteins 0.000 claims description 13
- 102100023321 Ceruloplasmin Human genes 0.000 claims description 13
- 238000003908 quality control method Methods 0.000 claims description 12
- 241001494479 Pecora Species 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 5
- 239000010931 gold Substances 0.000 claims description 5
- 229910052737 gold Inorganic materials 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 238000012549 training Methods 0.000 abstract description 3
- 239000012528 membrane Substances 0.000 abstract 4
- 238000010521 absorption reaction Methods 0.000 abstract 3
- 238000012544 monitoring process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 239000002689 soil Substances 0.000 description 7
- 241000700605 Viruses Species 0.000 description 6
- 206010012735 Diarrhoea Diseases 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 206010012742 Diarrhoea infectious Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 208000001848 dysentery Diseases 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 241000714209 Norwalk virus Species 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 206010004016 Bacterial diarrhoea Diseases 0.000 description 2
- 208000005577 Gastroenteritis Diseases 0.000 description 2
- 241001466963 Hawaii calicivirus Species 0.000 description 2
- 241000369757 Sapovirus Species 0.000 description 2
- 241001502514 Small round structured virus Species 0.000 description 2
- 241000509413 Snow Mountain virus Species 0.000 description 2
- 241000714208 Southampton virus Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 241000714198 Caliciviridae Species 0.000 description 1
- 240000004859 Gamochaeta purpurea Species 0.000 description 1
- 241001617329 Norovirus isolates Species 0.000 description 1
- 241000369774 Norwalk-like virus Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 201000009840 acute diarrhea Diseases 0.000 description 1
- 208000012873 acute gastroenteritis Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The utility model provides a norovirus detection kit. The kit comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, water absorption filter paper and a reaction supporter, wherein the sample pad is arranged at the bottom end of the reaction supporter; the top end of the sample pad covers the bottom end of the colloidal gold pad and is bonded with the bottom end of the colloidal gold pad; the top end of the colloidal gold pad covers the bottom end of the nitrocellulose membrane and is bonded with the bottom end of the nitrocellulose membrane; the top end of the nitrocellulose membrane is covered by the bottom end of the water absorption filter paper and bonded with the bottom end of the water absorption filter paper. The kit provided by the utility model is capable of simplifying the detection flow for norovirus in an excrement sample or a vomitus sample. The detection on the norovirus by using the detection kit is free from dependence on any experimental instrument, environments or operating workers, easy to operate, convenient, short in detection period, and easy to interpret; in addition, special instrument equipment and special training are not needed, and the kit is high in adaptability and can be used for conveniently monitoring and inspecting any time anywhere.
Description
Technical field
The utility model relates to field of biological detection, is specifically related to a kind of norovirus detection kit.
Background technology
Norwalk virus (Norwalk Viruses, NV) is the prototype representative strains that in mankind's Caliciviridae (Human Calicivirus, HuCV), norovirus (Norovirus, NV) belongs to.The virion that NV is one group of plesiomorphism, antigenicity is slightly different.Norwalk virus is the cause of disease separating patient's ight soil of an acute diarrhea breaking out in Cécile Nowak city of the U.S. from nineteen sixty-eight the earliest.After this, in gastroenteritis patient ight soil, isolate variform similarly successively all over the world but the slightly different virus-like particle of antigenicity, all name with location, as: Hawaii Virus (HV), Snow Mountain Virus (SMV), Mexico Virus (MxV) Southampton Virus (SOV) etc., be called before this small round structured virus (Small Round StructuralVirus, SRSV), after be called norwalk group viruses (Norwalk-like virus, NLV).Until August in 2002, the 8th ICNV's approved name was called norovirus (Norovirus, NV).Norovirus and the Sapporo sample virus (Sapporo-like Virus, SLV) of finding in Japan, present formal name is called letter as virus (Sapovirus, SV), is collectively referred to as mankind's calicivirus.
NV has many common traits: diameter is about 26～35nm, without coating, and rough surface, spherical, be icosahedron symmetry; From acute gastroenteritis patient's ight soil, separate, can not in cell or tissue, cultivate, also there is no suitable animal model; Genome is sub-thread positive chain RNA; Buoyant density in cesium chloride density gradient is 1.36～1.41g/cm
3; Under Electronic Speculum, lack significant morphological feature, the demonstration of negative staining electromicroscopic photograph, NV has typical pinniform outer rim, surperficial pitted roundlet shape structure virus.
Norovirus infectious diarrhea is to belong to by norovirus the diarrhoea that virus causes, has the features such as morbidity is anxious, velocity of propagation is fast, coverage is wide, is the Etiological that causes that Non-bacterial diarrhea breaks out.Norovirus is infectious strong, taking intestinal transmitted as main, can be by propagation such as the water source of pollution, food, article, air, and the community that is everlasting, school, restaurant, hospital, nursery, old folks' home and army etc. locate to cause that collective breaks out.
Norovirus heredity highly makes a variation, in the same period with may to have the strain that hereditary capacity is different in same community popular.Norovirus antibody does not have significant protective effect, especially there is no permanent immunity protective effect, very easily causes repeated infection.
Norovirus infectious diarrhea all has popular in worldwide, and all can infect the whole year, and infecting object is mainly adult and school-ager, and present cold season occurred frequently.The U.S. is every year in all Non-bacterial diarrheas break out, and 60-90% is caused by norovirus.Also there is similar results in the developed countries such as Holland, Britain, Japan, Australia.In developing country, norovirus infectious diarrhea ubiquity, also often causes outbreak of epidemic.In China, below 5 years old in diarrhea children, norovirus recall rate is 15% left and right, and antibody level of serum investigation shows that the infection of norovirus in population of China is also very general.Nineteen ninety-five, China has reported that the first norovirus infects, and a lot of norovirus infectious diarrhea epidemic outbreaks occur the area priority such as Shanxi, Beijing, Anhui, Foochow, Wuhan, Guangzhou afterwards.
At present, the detection method of the main application of this project detection is: the methods such as direct electron microscopy (EM), immunoelectron microscopic method (IEM), radioimmunology (RIA), biotin-avidin immunization (Biotin-Avidin Immunoassy) and euzymelinked immunosorbent assay (ELISA) (ELISA), hybridization technique and RT-polymerase chain reaction (RT-PCR) detect.But these detection methods all need to rely on corresponding instrument and equipment, and need to use liquid reagent etc., be not easy to detect on the spot, and the preservation of reagent and using is also restricted, be also not easy to the fast and convenient experimental result that obtains.
Thereby, need at present a kind of method that can diagnose early stage, quick, easy, reliably norovirus.
Utility model content
For solving the problems of the technologies described above, the utility model provides a kind of norovirus detection kit.
The technical solution adopted in the utility model is as follows:
The utility model provides a kind of norovirus detection kit, comprises sample pad, collaurum pad, nitrocellulose filter, absorbent filter and reaction holder; Wherein, described sample pad, described collaurum pad, described nitrocellulose filter, described absorbent filter are successively set on described reaction holder; Described sample pad is arranged on the bottom of described reaction holder, the top of described sample pad is pushed down the bottom of described collaurum pad and is pasted with it, the top of described collaurum pad is pushed down the bottom of described nitrocellulose filter and is pasted with it, and the top of described nitrocellulose filter is pushed down by the bottom of described absorbent filter and pasted with it.
Preferably, described absorbent filter length is 33.0mm, and the overlapping overlap length being pasted together of described absorbent filter and described nitrocellulose filter is 1.0mm.
Preferably, the length of described collaurum pad is 6.0mm, and the overlapping overlap length being pasted together of described collaurum pad and described nitrocellulose filter is 1.0mm.
Preferably, the length of described sample pad is 25.0mm, and described sample pad and the overlapping overlap length being pasted together of described collaurum pad are 2.0mm.
Preferably, described collaurum pad is the collaurum pad of the norovirus capsid protein monoclonal antibody that contains colloid gold label.
Preferably, described nitrocellulose filter is provided with test strip and Quality Control band, described test strip is arranged near a side of described collaurum pad and contains norovirus capsid protein monoclonal antibody, and described Quality Control band is arranged at away from a side of described collaurum pad and contains sheep anti-mouse igg polyclonal antibody.
Preferably, described reaction holder is plastics.
The utility model provides a kind of method of applying norovirus detection kit and carrying out norovirus detection, comprises the following steps:
(1) by sample to be checked dilution processing; Wherein, described sample to be checked is ight soil or vomitus;
(2) sample drop of having diluted is added in the sample pad of norovirus detection kit; Sample will move to the direction of absorbent filter along each attachment reacting on holder successively;
(3), if contain norovirus in described sample, the norovirus capsid protein in norovirus, through in the process of described collaurum pad, is combined with the norovirus capsid protein monoclonal antibody specificity of described collaurum pad mark and is formed compound; Described compound is through in the process of described nitrocellulose filter, successively with respect to described compound norovirus capsid protein monoclonal antibody in shortage and sheep anti-mouse igg polyclonal antibody specific binding; There is redness or pink in test strip place, and sample is positive;
If do not contain norovirus in described sample, there is not redness or pink in test strip place, and sample is negative;
(4) red or pink if Quality Control band place occurs, show that experimental result is effective; If there is not redness or pink in Quality Control band place, show that collaurum lost efficacy or experimental implementation is wrong, experimental result is invalid.
Preferably, in step (1), the 0.02M phosphate buffer of the NP40 of use 2% and 0.5% neopelex, as sample diluting liquid, dilutes sample to be checked.
The beneficial effects of the utility model are as follows:
The norovirus detection kit that the utility model provides, can simplify the testing process of norovirus in ight soil or vomitus sample.Utilize this kind of detection kit to detect norovirus, any experimental apparatus, environment or operating personnel are not had to dependence, simple, convenient, sense cycle is short, be easy to interpretation; In addition, do not need special instrument and equipment, do not need professional training, strong adaptability, is convenient to monitor anywhere or anytime and check.
Detection method that the utility model provides is simple to operation, do not rely on higher laboratory condition, for fast detecting norovirus, and then realize the cause of disease of the relevant diseases such as fast and convenient auxiliary diagnosis diarrhoea.
Brief description of the drawings
Fig. 1 is the front schematic view of a detection kit of the present utility model;
Fig. 2 is the side schematic view of a detection kit of the present utility model;
Fig. 3 is the positive schematic diagram of a detection kit testing result of the present utility model;
Fig. 4 is the negative schematic diagram of a detection kit testing result of the present utility model;
Fig. 5 is an invalid schematic diagram of detection kit result of the present utility model;
Fig. 6 is an invalid schematic diagram of detection kit result of the present utility model.
Wherein: 1, absorbent filter; 2, nitrocellulose filter; 3, collaurum pad; 4, sample pad; 5, reaction holder; T, test strip; C, Quality Control band.
Embodiment
Below in conjunction with accompanying drawing, the utility model is elaborated:
The utility model embodiment is the concrete detection mode for GII.4 norovirus, and other hypotypes or genotypic detection method all can obtain detection method according to following thinking making after suitable monoclonal antibody.
The norovirus detection kit that the utility model provides is made up of absorbent filter 1, nitrocellulose filter 2, collaurum pad 3, sample pad 4 and reaction holder 5.Wherein, reaction holder can be plastics.Absorbent filter can prevent that sample is in one end of nitrocellulose filter accumulation, thereby causes follow-up sample cannot continue to move ahead, and then can react fully.Nitrocellulose filter 2 comprises the test strip T that contains norovirus capsid protein monoclonal antibody (immunogene is VLP GII.4, and antibody is numbered 17E11) and the Quality Control band C that contains sheep anti-mouse igg polyclonal antibody.The norovirus capsid protein monoclonal antibody (immunogene is VLP GII.4, and antibody is numbered 10C07) that collaurum pad 3 contains colloid gold label.The nitrocellulose filter 2 of coated test strip T and Quality Control band C sticks on reaction holder 5.
Concrete, sample pad is arranged on the bottom of reaction holder, and the top of sample pad is pushed down the bottom of collaurum pad and is pasted with it, and the length of sample pad is 25.0mm, and described sample pad and the overlapping overlap length being pasted together of described collaurum pad are 2.0mm.
The top of collaurum pad is pushed down the bottom of nitrocellulose filter and is pasted with it, and the length of collaurum pad is 6.0mm, and the overlapping overlap length being pasted together of described collaurum pad and described nitrocellulose filter is 1.0mm.
The top of nitrocellulose filter is pushed down by the bottom of absorbent filter and is pasted with it, and absorbent filter length is 33.0mm, and the overlapping overlap length being pasted together of absorbent filter and nitrocellulose filter is 1.0mm.
The Edge Distance of nitrocellulose filter reaction holder edge 28.0mm in the direction of test strip T, the edge 32.0mm of the Edge Distance of nitrocellulose filter reaction holder in the direction of Quality Control band C.
In order to reach the object that detects norovirus capsid protein, need to select suitable dilution formula, thereby the capsid protein of norovirus virus coat can be separated, what in this example, use is that the 0.02M phosphate buffer of the neopelex that contains 2% NP40 and 0.5% is as sample preparation liquid.
The sample preparation liquid to be checked of 50～80 μ l is directly dropped to detection kit sample pad, and now sample, by moving to the direction of absorbent filter along each attachment reacting on holder 5 successively, reads experimental result in 10 minutes.
If when the norovirus containing in sample to be checked (ight soil or vomitus), the norovirus capsid protein monoclonal antibody specificity of the colloid gold label in detection kit is combined and is formed compound, then continue to move ahead, with another norovirus capsid protein monoclonal antibody generation specific binding being coated on nitrocellulose filter 2, occur redness or pink at test strip place, i.e. detection line, shows to contain norovirus in sample to be checked, positive, with reference to Fig. 3.
If what contain in sample to be checked does not contain norovirus, just can not form enough compounds, it is red or pink, negative that test strip place also just can not outlet, with reference to Fig. 4.
No matter in sample, whether contain norovirus, the monoclonal antibody of above-mentioned colloid gold label all can continue to proceed to Quality Control band and be combined with coated herein sheep anti-mouse igg polyclonal antibody, forms red or pink band, is nature controlling line.If this band does not occur in detection, prove that collaurum lost efficacy or operation makes mistakes, result is invalid, need to again detect, with reference to Fig. 5 and Fig. 6.
The norovirus detection kit that the utility model provides, can simplify the testing process of norovirus in ight soil or vomitus sample.Utilize this kind of detection kit to detect norovirus, any experimental apparatus, environment or operating personnel are not had to dependence, simple, convenient, sense cycle is short, be easy to interpretation; In addition, do not need special instrument and equipment, do not need professional training, strong adaptability, is convenient to monitor anywhere or anytime and check.
Detection method that the utility model provides is simple to operation, do not rely on higher laboratory condition, for fast detecting norovirus, and then realize the cause of disease of the relevant diseases such as fast and convenient auxiliary diagnosis diarrhoea.
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be looked protection domain of the present utility model.
Claims (1)
1. a norovirus detection kit, is characterized in that, comprises sample pad, collaurum pad, nitrocellulose filter, absorbent filter and reaction holder; Wherein, described sample pad, described collaurum pad, described nitrocellulose filter, described absorbent filter are successively set on described reaction holder; Described sample pad is arranged on the bottom of described reaction holder, the top of described sample pad is pushed down the bottom of described collaurum pad and is pasted with it, the top of described collaurum pad is pushed down the bottom of described nitrocellulose filter and is pasted with it, and the top of described nitrocellulose filter is pushed down by the bottom of described absorbent filter and pasted with it;
Wherein, described absorbent filter length is 33.0mm, and the overlapping overlap length being pasted together of described absorbent filter and described nitrocellulose filter is 1.0mm;
Wherein, the length of described collaurum pad is 6.0mm, and the overlapping overlap length being pasted together of described collaurum pad and described nitrocellulose filter is 1.0mm;
Wherein, the length of described sample pad is 25.0mm, and described sample pad and the overlapping overlap length being pasted together of described collaurum pad are 2.0mm;
Wherein, described collaurum pad is the collaurum pad of the norovirus capsid protein monoclonal antibody that contains colloid gold label;
Wherein, described nitrocellulose filter is provided with test strip and Quality Control band, described test strip is arranged near a side of described collaurum pad and contains norovirus capsid protein monoclonal antibody, and described Quality Control band is arranged at away from a side of described collaurum pad and contains sheep anti-mouse igg polyclonal antibody;
Wherein, the Edge Distance of nitrocellulose filter reaction holder edge 28.0mm in the direction of test strip T, the edge 32.0mm of the Edge Distance of nitrocellulose filter reaction holder in the direction of Quality Control band C;
Wherein, described reaction holder is plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320705300.0U CN203759021U (en) | 2013-11-08 | 2013-11-08 | Norovirus detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320705300.0U CN203759021U (en) | 2013-11-08 | 2013-11-08 | Norovirus detection kit |
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| Publication Number | Publication Date |
|---|---|
| CN203759021U true CN203759021U (en) | 2014-08-06 |
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| CN201320705300.0U Expired - Lifetime CN203759021U (en) | 2013-11-08 | 2013-11-08 | Norovirus detection kit |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016082162A1 (en) * | 2014-11-27 | 2016-06-02 | Kimberly-Clark Worldwide, Inc. | Devices and methods for detecting norovirus on surfaces |
| CN105717294A (en) * | 2014-12-16 | 2016-06-29 | 生物梅里埃公司 | Method and device for determining the presence of a micro-organism in stools with activated carbon pretreatment |
| CN109957014A (en) * | 2017-12-25 | 2019-07-02 | 中国科学院上海巴斯德研究所 | The preparation and application of anti-norovirus GII.3 source of mouse monoclonal antibody |
| CN110456041A (en) * | 2019-08-06 | 2019-11-15 | 珠海市医友生物科技有限公司 | A kind of norovirus GI type GII type combined detection reagent and preparation method thereof |
| CN110726839A (en) * | 2019-11-18 | 2020-01-24 | 江苏纳迪芯生命科技研究院有限公司 | Mumps virus IgM antibody gold-labeled detection kit and detection method |
-
2013
- 2013-11-08 CN CN201320705300.0U patent/CN203759021U/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016082162A1 (en) * | 2014-11-27 | 2016-06-02 | Kimberly-Clark Worldwide, Inc. | Devices and methods for detecting norovirus on surfaces |
| KR20170086060A (en) * | 2014-11-27 | 2017-07-25 | 킴벌리-클라크 월드와이드, 인크. | Devices and methods for detecting norovirus on surfaces |
| GB2547600A (en) * | 2014-11-27 | 2017-08-23 | Kimberly Clark Co | Devices and methods for detecting norovirus on surfaces |
| US10209256B2 (en) | 2014-11-27 | 2019-02-19 | Kimberly-Clark Worldwide, Inc. | Devices and methods for detecting norovirus on surfaces |
| GB2547600B (en) * | 2014-11-27 | 2021-02-10 | Kimberly Clark Co | Devices and methods for detecting norovirus on surfaces |
| KR102268785B1 (en) * | 2014-11-27 | 2021-06-25 | 킴벌리-클라크 월드와이드, 인크. | Devices and methods for detecting norovirus on surfaces |
| CN105717294A (en) * | 2014-12-16 | 2016-06-29 | 生物梅里埃公司 | Method and device for determining the presence of a micro-organism in stools with activated carbon pretreatment |
| CN109957014A (en) * | 2017-12-25 | 2019-07-02 | 中国科学院上海巴斯德研究所 | The preparation and application of anti-norovirus GII.3 source of mouse monoclonal antibody |
| CN109957014B (en) * | 2017-12-25 | 2022-03-29 | 中国科学院上海巴斯德研究所 | Preparation and application of anti-norovirus GII.3 murine monoclonal antibody |
| CN110456041A (en) * | 2019-08-06 | 2019-11-15 | 珠海市医友生物科技有限公司 | A kind of norovirus GI type GII type combined detection reagent and preparation method thereof |
| CN110726839A (en) * | 2019-11-18 | 2020-01-24 | 江苏纳迪芯生命科技研究院有限公司 | Mumps virus IgM antibody gold-labeled detection kit and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: JIANG BINGZE Effective date: 20150601 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150601 Address after: 102629 Beijing City, Daxing District science and Technology Park of Zhongguancun Daxing biomedical industry base of Yongxing Road No. 25 Patentee after: BEIJING EASYSWEET BIOMEDICINE SCITECH Co.,Ltd. Address before: 102629 Beijing City, Daxing District science and Technology Park of Zhongguancun Daxing biomedical industry base of Yongxing Road No. 25 Patentee before: BEIJING EASYSWEET BIOMEDICINE SCITECH Co.,Ltd. Patentee before: Jiang Bingze |
|
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 102629 Beijing City, Daxing District science and Technology Park of Zhongguancun Daxing biomedical industry base of Yongxing Road No. 25 Patentee after: Beijing Easysweet Biotechnology Co.,Ltd. Address before: 102629 Beijing City, Daxing District science and Technology Park of Zhongguancun Daxing biomedical industry base of Yongxing Road No. 25 Patentee before: BEIJING EASYSWEET BIOMEDICINE SCITECH Co.,Ltd. |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140806 |