CN203732498U - Electrochemical immunosensor for quickly detecting naphthalene content in water - Google Patents
Electrochemical immunosensor for quickly detecting naphthalene content in water Download PDFInfo
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- CN203732498U CN203732498U CN201420041334.9U CN201420041334U CN203732498U CN 203732498 U CN203732498 U CN 203732498U CN 201420041334 U CN201420041334 U CN 201420041334U CN 203732498 U CN203732498 U CN 203732498U
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Abstract
The utility model provides an electrochemical immunosensor for quickly detecting naphthalene content in water, which is composed of a tri-electrode electrochemical system, a conversion circuit, a singlechip and a display unit, wherein a naphthalene antibody is cured on a work electrode of the tri-electrode electrochemical system. The conversion circuit comprises a constant potential circuit, an amplification circuit, a digital-analog conversion circuit, an I/V conversion circuit, a low-pass filter circuit and an a analog-to-digital conversion circuit; the constant potential circuit is connected between a reference electrode and the work electrode of the tri-electrode electrochemical system; and the constant potential circuit is connected with the singlechip through the amplification circuit and digital-analog conversion circuit. An auxiliary electrode is connected with the singlechip through the I/V conversion circuit, low-pass filter circuit and analog-to-digital conversion circuit. Due to the adoption of the technical scheme, the electrochemical immunosensor implements quick detection of naphthalene content in water, has the advantages of favorable selectivity, high sensitivity, short response time and simple apparatuses, and is convenient for operation and popularization.
Description
Technical field
The utility model belongs to electrochemical immunosensor technical field, especially relates to a kind of electrochemical immunosensor for fast detecting water naphthalene content.
Background technology
Along with the aggravation of environmental pollution, the content of carcinogen palycyclic aromatic constantly increases, and becomes one of healthy main matter of harm people.The kind of palycyclic aromatic is a lot, and wherein naphthalene content maximum, is generally present in water sweetening of the soil.
In existing technology, the detection method of the content of palycyclic aromatic all adopts chemical method, and chemical method detection speed is slow, and the data that obtain have one-sidedness and inaccuracy, now need to provide a kind of fast for detection of the device of naphthalene concentration content in water.
Summary of the invention
Problem to be solved in the utility model is to provide a kind of electrochemical immunosensor for fast detecting water naphthalene content, is especially suitable for detecting naphthalene concentration content in water, provides foundation for administering to pollute.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is:
For the electrochemical immunosensor of fast detecting water naphthalene content, it is characterized in that: formed by three-electrode electro Chemical system, change-over circuit, single-chip microcomputer and display unit; Described three-electrode electro Chemical system is communicated and is connected with single-chip microcomputer by change-over circuit, and described display unit connects single-chip microcomputer; On the working electrode of described three-electrode electro Chemical system, solidify naphthalene antibody.
Further, described change-over circuit comprises potentiostatic circuit, amplifying circuit, D/A converting circuit, I/V change-over circuit, low-pass filter circuit and analog to digital conversion circuit,
Between the contrast electrode of described three-electrode electro Chemical system and working electrode, connect potentiostatic circuit, potentiostatic circuit connects single-chip microcomputer by amplifying circuit, D/A converting circuit; Auxiliary electrode connects single-chip microcomputer by I/V change-over circuit, low-pass filter circuit, analog to digital conversion circuit.
Further, the working electrode of described three-electrode electro Chemical system is that quartz crystal gold electrode, contrast electrode are that mercurous chloride electrode, auxiliary electrode are platinum electrode, and electrolytic solution is the potpourri of the potassium ferricyanide, potassium ferrocyanide, KCL and PBS.
Further, described three-electrode electro Chemical system is current mode three-electrode electro Chemical system.
Advantage and the good effect that the utlity model has are:
Owing to adopting technique scheme, realize the fast detecting of naphthalene content in water, selectivity is good, highly sensitive, the response time is short; And instrument is simple, easy to operate, be convenient to promote the use of.
Brief description of the drawings
Fig. 1 is circuit module block diagram of the present utility model;
Fig. 2 is the schematic diagram of three-electrode electro Chemical system of the present utility model;
Fig. 3 is potentiostatic circuit figure of the present utility model and amplification circuit diagram;
Fig. 4 is I/V change-over circuit figure of the present utility model;
Fig. 5 is low-pass filter circuit figure of the present utility model;
Fig. 6 is D/A converting circuit figure of the present utility model.
Embodiment
Electrochemical immunosensor for fast detecting water naphthalene content of the present utility model, is made up of three-electrode electro Chemical system, change-over circuit, single-chip microcomputer and display unit;
Single-chip microcomputer adopts IAP15F2K61S2 chip, is the core of controlling, and realizes collection, processing, storage and the demonstration of data, simultaneously provide-0.8-0.8V analog voltage signal;
As shown in Figure 2; the preferred current mode three-electrode electro Chemical of described three-electrode electro Chemical system system; its working electrode WE is that quartz crystal gold electrode, contrast electrode RE are that mercurous chloride electrode, auxiliary electrode AE are platinum electrode; electrolytic solution is the potpourri of the potassium ferricyanide, potassium ferrocyanide, KCL and PBS, the upper naphthalene antibody that solidifies of working electrode WE;
As shown in Figure 1, described change-over circuit comprises D/A converting circuit, amplifying circuit, potentiostatic circuit, I/V change-over circuit, low-pass filter circuit and analog to digital conversion circuit;
Between the contrast electrode RE of described three-electrode electro Chemical system and working electrode WE, connect potentiostatic circuit, single-chip microcomputer puts on three-electrode electro Chemical sensor by D/A converting circuit, amplifying circuit and potentiostatic circuit by external drive signal successively exactly; Auxiliary electrode AE gathers current signal, inputs single-chip microcomputer and carry out, after analyzing and processing, finally by display unit, data being shown after IV change-over circuit, low-pass filter circuit, D/A converting circuit;
It is constant that described potentiostatic circuit is used between maintenance work electrode WE and contrast electrode RE potential difference (PD), as shown in Figure 3, potentiostatic circuit comprises control amplifier, contrast electrode RE current potential is added to the end of oppisite phase of control amplifier, control amplifier in-phase input end ground connection as reference potential, the output termination auxiliary electrode AE of control amplifier forms close loop negative feedback regulating system, in electrode when curent change, contrast electrode RE is with respect to any subtle change of working electrode WE current potential, all the voltage negative feedback by circuit is corrected, thereby reach the object of automatic constant current potential,
Single-chip microcomputer is converted to simulating signal by D/A converting circuit by digital signal by pumping signal, and after amplifying by amplifying circuit by potentiostatic circuit, pumping signal is added on and on three-electrode electro Chemical sensor, drives sample solution generation electrochemical reaction the most at last; Wherein D/A converting circuit as shown in Figure 6, is to adopt TLV5638 chip, is 12 D/A converters of TI company, have two output channels, data transmission interface is the serial line interface of 3 lines, transmits data at every turn and forms a frame by the data of 16, wherein 4 control command words, 12 output data;
After potentiostatic circuit makes the voltage maintenance between working electrode and contrast electrode constant, gather current signal by auxiliary electrode AE, after IV change-over circuit, low-pass filter circuit, D/A converting circuit, input single-chip microcomputer IAP15F2K61S2 and carry out computing, and demonstrate result by display unit;
Concrete circuit embodiment is as follows:
Described I/V change-over circuit as shown in Figure 4, is made up of LM324 operational amplifier, and Main Function is that the current input signal that auxiliary electrode AE is gathered converts Voltage-output signal to.Because the input impedance of operational amplifier is much larger than output impedance, thereby play and the effect of single main circuit isolation, avoided the excessive chip that burns out single main circuit of electric current because of input.Voltage swing that can regulation output by resistance R 1, the voltage that prevents conversion is excessive and exceed the voltage that AD transformation energy bears;
As shown in Figure 5, its effect is only to allow the voltage signal after IV conversion to pass through to low-pass filter circuit, and other undesired signal is filtered out.Second-order low-pass filter circuit, by increasing RC link, narrows the transitional zone of wave filter, and attenuation slope value strengthens, and has better reduced input voltage.
The course of work of this example:
The aquaculture water that contains a certain amount of naphthalene is joined in sample cell, three electrodes of three-electrode electro Chemical system are put into solution to be measured, by Single-chip Controlling, provide-0.8-0.8V of three electrodes analog voltage signal is provided, by potentiostatic circuit, external drive signal is put on three-electrode electro Chemical sensor exactly, drive sample solution generation electrochemical reaction, and the response signal producing is carried out being sent to single-chip microcomputer after IV conversion, amplification, filtering, AD conversion, find peak current through calculating, record cyclic voltammetry curve.According to the variation of cyclic voltammetry curve cathodic peak current, can measure the content of naphthalene in solution, and show in 12864 LCDs.
After each end of scan, the PBS buffer solution that the electrode of three-electrode electro Chemical system is placed in, upgrades electrode surface, makes electrode have good reappearance.
Above an embodiment of the present utility model is had been described in detail, but described content is only preferred embodiment of the present utility model, can not be considered to for limiting practical range of the present utility model.All equalization variation and improvement etc. of doing according to the utility model application range, within all should still belonging to patent covering scope of the present utility model.
Claims (4)
1. for the electrochemical immunosensor of fast detecting water naphthalene content, it is characterized in that: formed by three-electrode electro Chemical system, change-over circuit, single-chip microcomputer and display unit; Described three-electrode electro Chemical system is communicated and is connected with single-chip microcomputer by change-over circuit, and described display unit connects single-chip microcomputer; On the working electrode of described three-electrode electro Chemical system, solidify naphthalene antibody.
2. the electrochemical immunosensor for fast detecting water naphthalene content according to claim 1, it is characterized in that: described change-over circuit comprises potentiostatic circuit, amplifying circuit, D/A converting circuit, I/V change-over circuit, low-pass filter circuit and analog to digital conversion circuit
Between the contrast electrode of described three-electrode electro Chemical system and working electrode, connect potentiostatic circuit, potentiostatic circuit connects single-chip microcomputer by amplifying circuit, D/A converting circuit; Auxiliary electrode connects single-chip microcomputer by I/V change-over circuit, low-pass filter circuit, analog to digital conversion circuit.
3. the electrochemical immunosensor for fast detecting water naphthalene content according to claim 1 and 2, is characterized in that: the working electrode of described three-electrode electro Chemical system is that quartz crystal gold electrode, contrast electrode are that mercurous chloride electrode, auxiliary electrode are platinum electrode.
4. the electrochemical immunosensor for fast detecting water naphthalene content according to claim 1, is characterized in that: described three-electrode electro Chemical system is current mode three-electrode electro Chemical system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420041334.9U CN203732498U (en) | 2014-01-22 | 2014-01-22 | Electrochemical immunosensor for quickly detecting naphthalene content in water |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420041334.9U CN203732498U (en) | 2014-01-22 | 2014-01-22 | Electrochemical immunosensor for quickly detecting naphthalene content in water |
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| CN203732498U true CN203732498U (en) | 2014-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420041334.9U Expired - Fee Related CN203732498U (en) | 2014-01-22 | 2014-01-22 | Electrochemical immunosensor for quickly detecting naphthalene content in water |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106979969A (en) * | 2017-02-25 | 2017-07-25 | 深圳市赛亿科技开发有限公司 | The Intelligent bowl of concentration of heavy metal ion in a kind of detection food liquid |
-
2014
- 2014-01-22 CN CN201420041334.9U patent/CN203732498U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106979969A (en) * | 2017-02-25 | 2017-07-25 | 深圳市赛亿科技开发有限公司 | The Intelligent bowl of concentration of heavy metal ion in a kind of detection food liquid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140723 Termination date: 20150122 |
|
| EXPY | Termination of patent right or utility model |