CN203658263U - Mitochondrion membrane potential detection kit - Google Patents
Mitochondrion membrane potential detection kit Download PDFInfo
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- CN203658263U CN203658263U CN201320893255.6U CN201320893255U CN203658263U CN 203658263 U CN203658263 U CN 203658263U CN 201320893255 U CN201320893255 U CN 201320893255U CN 203658263 U CN203658263 U CN 203658263U
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- groove
- membrane potential
- reagent
- detection kit
- potential detection
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Abstract
The utility model discloses a mitochondrion membrane potential detection kit which comprises a kit body, a kit cover, a reagent liner, a JC-1 fluorescent probe, ultrapure water, a JC-1 dye buffer solution, and a CCCP (Carbonyl cyanide 3-chlorophenylhydrazone) inhibitor, wherein the kit body is made of hard plastics, the reagent liner made of hard plastic foam is arranged in the kit body, round grooves A, B and C are respectively formed in the reagent liner, the groove A is used for storing the JC-1 fluorescent probe, the groove B is used for containing ultrapure water and the JC-1 dye buffer solution, and the groove C is used for containing the CCCP inhibitor, and the kit cover is arranged at the top of the kit body. The mitochondrion membrane potential detection kit has the beneficial effects of being fast and convenient to use, small in volume, and easy to carry.
Description
Technical field
The utility model relates to biological technical field, is specially a kind of mitochondrial membrane potential detection kit.
Background technology
In the Apoptosis test experience to early stage, most benchmark that are changed to detection line mitochondrial membrane potential, but in the experiment of detection line mitochondrial membrane potential, change when too small in mitochondrial film potential, there will be experimental result to examine indeterminable situation, therefore, need to search out a kind of new material and carry out labeled mitochondria, but JC-1 is the desirable fluorescence probe that a kind of mitochondrial membrane potential that detects rapidly and sensitively cell, tissue or purifying changes, can detect for early stage Apoptosis, can detect the mitochondrial membrane potential of cell, tissue or purifying.In the time that mitochondrial membrane potential is higher, JC-1 is gathered in mitochondrial matrix, forms polymkeric substance, can produce red fluorescence; In the time that mitochondrial membrane potential is lower, JC-1 can not be gathered in mitochondrial matrix, and now JC-1 is monomer, can produce green fluorescence.So just can carry out easily the variation of detection line mitochondrial membrane potential by the transformation of fluorescence color.The relative scale of conventional red green fluorescence is weighed the ratio of mitochondrial depolarization.The decline of mitochondrial membrane potential is an early stage significant event of Apoptosis.Transformation by JC-1 from red fluorescence to green fluorescence can detect the decline of cell membrane potential easily, simultaneously also can be with the transformation of JC-1 from red fluorescence to green fluorescence as an early stage detection index of Apoptosis.
Utility model content
The purpose of this utility model is to provide the high access times of a kind of extracting concentration mitochondrial membrane potential detection kit of a specified duration, to solve the problem proposing in above-mentioned background technology.
For achieving the above object, the utility model provides following technical scheme: a kind of mitochondrial membrane potential detection kit, comprise box body, lid, reagent liner, JC-1 fluorescence probe, ultrapure water, the JC-1 damping fluid that dyes, CCCP inhibitor, described box body adopts rigid plastic to make, in box body, lay the reagent liner that rigid plastic foam is made, on reagent liner, have circular groove A, groove B, groove C, groove A is used for depositing JC-1 fluorescence probe, groove B is placed with respectively ultrapure water, the JC-1 damping fluid that dyes, in groove C for placing CCCP inhibitor, described lid is arranged on box body top.
In the utility model, described box body is cylindrical structural.
In the utility model, described JC-1 fluorescence probe reagent has five pipes.
In the utility model, the reagent bottle of described JC-1 fluorescence probe reagent is Brown Glass Brown glass bottles and jars only.
Compared with prior art, the beneficial effects of the utility model are: this mitochondrial membrane potential detection kit use quick and convenient, volume is small and exquisite, be easy to carry about with one, reagent liner adopts ramp designs, can not block the word on reagent bottle, greatly shorten the time of selecting reagent, reagent bottle is fallen into into box body inside simultaneously, has effectively solved the problem that fluorescence probe reagent must keep in Dark Place.
Accompanying drawing explanation
Fig. 1 is the utility model one-piece construction schematic diagram.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, rather than whole embodiment.Based on the embodiment in the utility model, those of ordinary skills are not making the every other embodiment obtaining under creative work prerequisite, all belong to the scope of the utility model protection.
Refer to Fig. 1, in the utility model embodiment, a kind of mitochondrial membrane potential detection kit, comprise box body 1, lid 2, reagent liner 3, JC-1 fluorescence probe 4, ultrapure water 5, the JC-1 damping fluid 6 that dyes, CCCP inhibitor 7, described box body 1 adopts rigid plastic to make, in box body 1, lay the reagent liner 3 that rigid plastic foam is made, on reagent liner 3, have circular groove A, groove B, groove C, groove A is used for depositing JC-1 fluorescence probe 4, groove B is placed with respectively ultrapure water 5, the JC-1 damping fluid 6 that dyes, in groove C for placing CCCP inhibitor 7, described lid 2 is arranged on box body 1 top.
In the utility model, described box body 1 is cylindrical structural.
In the utility model, described JC-1 fluorescence probe 4 reagent have five pipes.
In the utility model, the reagent bottle of described JC-1 fluorescence probe 4 reagent is Brown Glass Brown glass bottles and jars only.
To those skilled in the art, obviously the utility model is not limited to the details of above-mentioned one exemplary embodiment, and in the situation that not deviating from spirit of the present utility model or essential characteristic, can realize the utility model with other concrete form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present utility model is limited by claims rather than above-mentioned explanation, is therefore intended to all changes that drop in the implication and the scope that are equal to important document of claim to include in the utility model.Any Reference numeral in claim should be considered as limiting related claim.
Claims (4)
1. a mitochondrial membrane potential detection kit, it is characterized in that: comprise box body, lid, reagent liner, JC-1 fluorescence probe, ultrapure water, JC-1 dyeing damping fluid, CCCP inhibitor, described box body adopts rigid plastic to make, in box body, lay the reagent liner that rigid plastic foam is made, on reagent liner, have circular groove A, groove B, groove C, groove A is used for depositing JC-1 fluorescence probe, groove B is placed with respectively ultrapure water, the JC-1 damping fluid that dyes, in groove C, for placing CCCP inhibitor, described lid is arranged on box body top.
2. a kind of mitochondrial membrane potential detection kit according to claim 1, is characterized in that: described box body is cylindrical structural.
3. a kind of mitochondrial membrane potential detection kit according to claim 1, is characterized in that: described JC-1 fluorescence probe reagent has five pipes.
4. a kind of mitochondrial membrane potential detection kit according to claim 1, is characterized in that: the reagent bottle of described JC-1 fluorescence probe reagent is Brown Glass Brown glass bottles and jars only.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320893255.6U CN203658263U (en) | 2013-12-31 | 2013-12-31 | Mitochondrion membrane potential detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320893255.6U CN203658263U (en) | 2013-12-31 | 2013-12-31 | Mitochondrion membrane potential detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203658263U true CN203658263U (en) | 2014-06-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320893255.6U Expired - Fee Related CN203658263U (en) | 2013-12-31 | 2013-12-31 | Mitochondrion membrane potential detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203658263U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106885794A (en) * | 2017-03-06 | 2017-06-23 | 浙江星博生物科技股份有限公司 | A kind of human sperm's mitochondrial function detection method |
-
2013
- 2013-12-31 CN CN201320893255.6U patent/CN203658263U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106885794A (en) * | 2017-03-06 | 2017-06-23 | 浙江星博生物科技股份有限公司 | A kind of human sperm's mitochondrial function detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140618 Termination date: 20141231 |
|
| EXPY | Termination of patent right or utility model |