CN203625377U - Epidermal cell enrichment instrument - Google Patents
Epidermal cell enrichment instrument Download PDFInfo
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- CN203625377U CN203625377U CN201320763903.6U CN201320763903U CN203625377U CN 203625377 U CN203625377 U CN 203625377U CN 201320763903 U CN201320763903 U CN 201320763903U CN 203625377 U CN203625377 U CN 203625377U
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Abstract
The utility model discloses an epidermal cell enrichment instrument, belonging to the field of medical treatment. The epidermal cell enrichment instrument comprises a base, and a control panel, a digestion container, a filtering container, a mixing container, a control unit, a heater, a vibrator, a sensor, a power supply and the like which are arranged on the base, wherein the control panel comprises a display screen and keys, the vibrator is arranged at the bottom of the digestion container, the heater is arranged on the outer surface of the digestion container, and a temperature sensor is arranged on the digestion container; the heater and the vibrator are connected with the output end of the control unit respectively, and the control panel and the sensor are connected with the input end of the control unit respectively; the power supply supplies power to the control panel, the control unit, the heater and the vibrator respectively. The epidermal cell enrichment instrument disclosed by the utility model can rapidly disperse epidermis to obtain a high-density epidermal cell suspension.
Description
Technical field
The utility model relates to a kind of cell enrichment instrument, particularly relates to a kind of epidermic cell enriching instrument.
Background technology
In recent years, the sickness rate of China burn is total population 2 ‰～10 ‰, and nearly ten million people of burns victims occurs every year, need reaching more than hundreds of thousands of them of hospital care, burn is often left over a large amount of scars after curing, the appearance to patient, build, and function, psychological aspects cause negative impact.
At present, utilize limited skin source to treat larger area burn wound, mainly contain two kinds:
1, microparticle skin technology: use physical method to be dispersed into the little particulate of trying one's best in limited autologous skin source, then Hui Zhi is to patient's the surface of a wound, this technology can expand skin graft 10～20 times, but use this physical method to be difficult on the one hand skin graft further to expand, epidermis attaching rate is low on the other hand, is difficult to guarantee curative effect;
2, RECELL technology: because closing of the surface of a wound is that propagation by epidermal stem cells completes, RECELL technology is that stratum basale epidermic cell is carried out to static state digestion, then utilize spray method that epidermic cell is sprayed on to the surface of a wound, use in this technical know-how and skin graft can be expanded to 40～80 times, epidermal stem cells is mainly present in stratum basale, therefore, this technology is mainly to utilize epidermal stem cells to carry out wound repairing.Adopt static digestion mainly to have following defect: between digestion enzyme-to-substrate, mix not good enoughly, digestion time is long, and digests inhomogeneous.
In view of this, the inventor studies this, develops specially a kind of epidermic cell enriching instrument, and this case produces thus.
Utility model content
The purpose of this utility model is to provide a kind of epidermic cell enriching instrument, adopt vibrator to realize dynamically digestion, and then obtain high-density, great-hearted epidermic cell, and whole enriching instrument is simple in structure, easy to operate.
To achieve these goals, solution of the present utility model is:
A kind of epidermic cell enriching instrument, comprise base, and be arranged on control panel, digestion vessel, filtration vessel, mixing vessel, control unit, well heater, vibrator, sensor and the power supply etc. on base, wherein, control panel comprises display screen and button, vibrator is arranged on the bottom of digestion vessel, makes digestion vessel produce the vibration of certain frequency; Well heater is arranged on the outside surface of digestion vessel, for the liquid in hot digestion container, temperature sensor is arranged on digestion vessel, for monitoring the Heating temperature of digestion vessel, above-mentioned well heater, vibrator are connected with the output terminal of control unit respectively, control panel is connected with the input terminus of control unit respectively with sensor device, and power supply is respectively control panel, control unit, well heater and vibrator power supply.
The sidewall of above-mentioned digestion vessel is provided with a through hole, for mounting temperature sensor.
The upper end of above-mentioned filtration vessel is provided with filter screen, and for filtering dynamic postdigestive solution, the order number of filter screen is 20-60.
The inlet portion of above-mentioned digestion vessel, filtration vessel and mixing vessel and control panel are arranged on respectively on the upper surface of base.
On above-mentioned base upper surface, be further provided with an operator's console, for pre-treatment such as the shearings before epidermis digestion.
Described vibrator adopts vibrating motor, and speed range is 1100-2500rpm.
Control unit, power supply and vibrator are arranged on respectively on the lower surface of base, and the top of vibrator and the bottom connection of digestion vessel touch.
Compared with prior art, above-mentioned epidermic cell enriching instrument adopts vibrator to carry out dynamic digestion method, can fast discrete epidermis, obtain highdensity epidermal cell suspension, and whole enriching instrument is simple in structure, easy to operate.
Below in conjunction with drawings and the specific embodiments, the utility model is described in further detail.
Accompanying drawing explanation
Fig. 1 is the epidermic cell enriching instrument structural representation of the present embodiment;
Fig. 2 is the epidermic cell enriching instrument structural representation of the present embodiment;
Fig. 3 is the epidermic cell enriching instrument control module figure of the present embodiment;
Label declaration:
Filtration vessel 4;
Embodiment
As Figure 1-3, a kind of epidermic cell enriching instrument, comprise base 1, and be arranged on control panel 2, digestion vessel 3, filtration vessel 4, mixing vessel 5, control unit 6, well heater 7, vibrator 8, sensor 9 and the power supply 10 etc. on base 1, wherein, control panel 2 comprises display screen 21 and button 22, in the present embodiment, above-mentioned display screen 21 adopts charactron to show, be mainly used in the parameters such as displays temperature, vibrational frequency, cycling time, button 22 comprises power initiation button and vibration unlatching button.Vibrator 8 is arranged on the bottom of digestion vessel 3, makes digestion vessel 3 produce the vibration of certain frequency; In the present embodiment, described vibrator 8 adopts vibrating motor, and the speed range of vibrating motor is 1100-2500rpm.Vibrator 8 also can adopt other likes to substitute, as long as can be that solution in digestion vessel 3 produces the vibrations of certain frequency or rocks, as adopted shaking table to substitute vibrator 8, shaking speed general control be at 60-300rpm.Well heater 7 is arranged on the outside surface of digestion vessel 3, for hot digestion container 3, solution in digestion vessel 3 is remained between 36.8-37.5 ℃, sensor 9 is arranged on the through hole in the middle of digestion vessel 3 sidewalls, the probe of temperature sensor stretches in solution, the Heating temperature of Real-Time Monitoring digestion vessel 3, temperature sensor 9 can adopt the temperature sensor of Multiple Type, and object is accurately to sense the temperature of digestion vessel 3 interior solution.
Above-mentioned well heater 7, vibrator 8 are connected with the output terminal of control unit 6 respectively, and control panel 2 is connected with the input terminus of control unit 6 respectively with sensor 9, and power supply 10 is respectively control panel 2, control unit 6, well heater 7 and vibrator 8 and powers.In the present embodiment, above-mentioned control unit 6 adopts microprocessor, can select the microprocessor chip of multiple models, as long as can realize above-mentioned control function.
The upper end of filtration vessel 4 is provided with 50 object filtering nets.
The inlet portion of above-mentioned digestion vessel 3, filtration vessel 5 and mixing vessel 6 and control panel 2 are arranged on respectively on the upper surface 11 of base 1.The capacity of digestion vessel 3, filtration vessel 5 and mixing vessel 6 is generally 10-20ml.
On above-mentioned base upper surface 11, be further provided with an operator's console 13, before epidermis digestion, the pre-treatment such as shearing.
The dynamic enriching method that adopts above-mentioned epidermic cell enriching instrument, comprises pre-treatment, digestion, filtration and dosing, and concrete steps are:
(1) pre-treatment: get epidermis, make some epidermis sheets on the operator's console 13 of epidermic cell enriching instrument; The epidermis of getting is split-thickness skin graft, the intermediate split thickness skin graft that the thickness of split-thickness skin graft is 0.5mm, and the particle diameter of every of making epidermis sheet is at 3mm;
(2) digestion: start epidermic cell enriching instrument power supply 10, get step (1) gained epidermis sheet and be placed in digestion vessel 3, add digestive ferment, control vibrator 8 and well heater 7 by control unit 6, dynamically digest, obtain cell suspension; Digestive ferment is formed by pancreatin and EDTA configuration, and wherein, the concentration that the concentration of pancreatin is 0.4%, EDTA is 0.03%; Dynamically before digestion, to first leave standstill 6 minutes (also can be described as static digestion), and then Vibration on Start-up device 8, carry out dynamically digestion after 5 minutes, then leave standstill 6 minutes, restart under 8,37 ℃ of constant temperature of vibrator and circulate 4 times, after loop ends, carry out cell and wash away; The reagent that cell washes away employing is standard physiological saline, till going out digestion vessel completely with cell;
(3) filter: step (2) gained cell suspension is splashed into filtration vessel 4 and filter, remove epidermis residue, the enchylema of lower floor is for dosing; In the present embodiment, described filtering net is 50 orders;
(4) dosing: filtration gained enchylema is splashed into mixing vessel 5, mix with protein gelatin, mixed dosing.In the present embodiment, in lower floor's enchylema, add inhibitor and form cell suspension, inhibitor soybean trypsin, soybean trypsin adds with equimolar amount.Protein gelatin is Fibrin Glue, and mixing process is: respectively cell suspension is mixed with setting accelerator, Fibrin Glue.Preferably, setting accelerator and Fibrin Glue first carry out pre-mixing, again cell suspension and setting accelerator/Fibrin Glue are poured in the different cylinders of dual barrel syringe, under same pressure-acting, cell suspension and setting accelerator/Fibrin Glue are extruded simultaneously, mix dosing, the total amount of Fibrin Glue and setting accelerator and cell suspension equivalent.
Above-described embodiment and graphic and non-limiting product form of the present utility model and style, suitable variation or modification that any person of an ordinary skill in the technical field does it, all should be considered as not departing from patent category of the present utility model.
Claims (7)
1. an epidermic cell enriching instrument, it is characterized in that: comprise base, and be arranged on control panel, digestion vessel, filtration vessel, mixing vessel, control unit, well heater, vibrator, sensor and the power supply on base, wherein, control panel comprises display screen and button, vibrator is arranged on the bottom of digestion vessel, makes digestion vessel produce the vibration of certain frequency; Well heater is arranged on the outside surface of digestion vessel, for the liquid in hot digestion container, temperature sensor is arranged on digestion vessel, for monitoring the Heating temperature of digestion vessel, above-mentioned well heater, vibrator are connected with the output terminal of control unit respectively, control panel is connected with the input terminus of control unit respectively with sensor device, and power supply is respectively control panel, control unit, well heater and vibrator power supply.
2. a kind of epidermic cell enriching instrument as claimed in claim 1, is characterized in that: the sidewall of above-mentioned digestion vessel is provided with one for temperature sensor mounted through hole.
3. a kind of epidermic cell enriching instrument as claimed in claim 1, is characterized in that: the upper end of above-mentioned filtration vessel is provided with filter screen, and the order number of filter screen is 20-60.
4. a kind of epidermic cell enriching instrument as claimed in claim 1, is characterized in that: the inlet portion of above-mentioned digestion vessel, filtration vessel and mixing vessel and control panel are arranged on respectively on the upper surface of base.
5. a kind of epidermic cell enriching instrument as claimed in claim 1, is characterized in that: on above-mentioned base upper surface, be further provided with one for pretreated operator's console before epidermis digestion.
6. a kind of epidermic cell enriching instrument as claimed in claim 1, is characterized in that: control unit, power supply and vibrator are arranged on respectively on the lower surface of base, and the top of vibrator and the bottom connection of digestion vessel touch.
7. a kind of epidermic cell enriching instrument as claimed in claim 6, is characterized in that: described vibrator adopts vibrating motor, and speed range is 1100-2500rpm.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320763903.6U CN203625377U (en) | 2013-11-27 | 2013-11-27 | Epidermal cell enrichment instrument |
| PCT/CN2014/076037 WO2015078137A1 (en) | 2013-11-27 | 2014-04-23 | Epidermal cell collecting instrument and dynamic collecting method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320763903.6U CN203625377U (en) | 2013-11-27 | 2013-11-27 | Epidermal cell enrichment instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203625377U true CN203625377U (en) | 2014-06-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320763903.6U Expired - Fee Related CN203625377U (en) | 2013-11-27 | 2013-11-27 | Epidermal cell enrichment instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203625377U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110968033A (en) * | 2019-12-23 | 2020-04-07 | 深圳赛动生物自动化有限公司 | Stem cell liquid distribution bin control system based on programmable controller and control method thereof |
-
2013
- 2013-11-27 CN CN201320763903.6U patent/CN203625377U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110968033A (en) * | 2019-12-23 | 2020-04-07 | 深圳赛动生物自动化有限公司 | Stem cell liquid distribution bin control system based on programmable controller and control method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP03 | Change of name, title or address |
Address after: 312035 Zhejiang province Shaoxing city Gao Gao Bu Town North Industrial Zone Patentee after: ZHENDE MEDICAL Co.,Ltd. Address before: 312000 Zhejiang province Shaoxing city Gao Gao Bu Town North Industrial Zone Patentee before: SHAOXING ZHENDE SURGICAL DRESSING Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140604 Termination date: 20211127 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |