CN203561638U - Hole-shaped agar gel culture apparatus for immunodiffusion test - Google Patents
Hole-shaped agar gel culture apparatus for immunodiffusion test Download PDFInfo
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- CN203561638U CN203561638U CN201320727641.8U CN201320727641U CN203561638U CN 203561638 U CN203561638 U CN 203561638U CN 201320727641 U CN201320727641 U CN 201320727641U CN 203561638 U CN203561638 U CN 203561638U
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- agar gel
- culture apparatus
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Abstract
The utility model relates to the field of testing vessels, and particularly relates to a hole-shaped agar gel culture apparatus for an immunodiffusion test. The hole-shaped agar gel culture apparatus comprises a vessel body, a vessel cover and a punching pile which is fixedly connected to the bottom surface of the vessel cover, wherein the vessel cover and the vessel body are buckled; an interval is arranged between the bottom surface of the punching pile and the bottom surface of the vessel body, the punching pile is stretched into a melted agar solution in the vessel body, the agar solution is naturally cooled, the punching pile is taken out, a structured hole is formed in the agar gel, and the shape of the structured hole is same with that of the punching pile. The hole-shaped agar gel culture apparatus provided by the utility model has the advantages that the operation is simple in the process, and the agar gel is not easy to be polluted.
Description
Technical field
The utility model relates to tester field, relates in particular to a kind of poroid agar gel culture apparatus for immunodiffusion.
Background technology
SRID refers to and makes antigen and antibody free diffusing and meeting in medium under certain condition, thereby form the process of antigen antibody complex, in this process, this composite molecular weight increases and produces gathering, forms macroscopic band shape or wire sealed Belt when no longer continuing to spread.Along with scientific and technical progress, SRID is combined many new technology that produced with other technologies, as immunoelectrophoresis, immunorheophoresis electrophoresis, enzyme immunity diffusion etc., makes it to be more widely used in fields such as biology and medical science.
In immunodiffusion, need suitable medium to make dispersive medium, the advantage such as agar gel has that chemical stability is good, water cut is large, transparency good, convenient sources, processing are easy, and its inside is holey, aperture is very large, can allow macromolecular substances (molecular weight from tens0000 to more than millions of) freely to pass through (molecular weight of most of antigen and antibody is all more than 200,000), therefore agar gel is optimal dispersive medium in immunity diffusion detection technique.But SRID need to have poroid agar gel, in the method for existing preparation with poroid agar gel, operation steps is more, consuming time longer, and punching is cracking easily, finally makes test findings inaccurate, causes the waste of time and efforts.
At present, in preparation, be with in poroid agar gel process, the problem that we often run into has: 1, after agar solidifies, during with punching stake punching, because punching quantity is more, easy of forgetting calcination punching stake sterilizing on spirit lamp flame in operating process, make agar contaminated, make result unreliable; 2, when the agar with in syringe needle picking hole, syringe needle is easily drawn ceasma wall, makes hole cracking, and the liquid in hole spills, and affects the judgement of result; 3, when blind hole bottom, the time of toasting on flame is difficult for grasping, and paint baking over easily makes the bore deformation of having accomplished fluently, thus the result of impact experiment.
Application number is that 201010127238.2 Chinese patent discloses a kind of plum blossom type agar immunodiffusion test method and perforating device, and it comprises perforated pipe and a central punch pipe of blossom type; Perforated pipe and central punch pipe have respectively the negative pressure cavity for absorbing agar, are fixed with spiral needle in negative pressure cavity; The lower end of negative pressure cavity has opening, and the sidewall of the sidewall of perforated pipe and central punch pipe has hypotenuse blade at opening part; Each perforated pipe is fixedly connected with central punch pipe by the transverse tube of hollow, in central punch pipe upper end, is provided with rubber negative pressure sac, and the negative pressure cavity of each perforated pipe is connected with rubber negative pressure sac.This scheme can realize on agar gel once property and get the combination bore with blossom type, improved to a certain extent the efficiency of preparing agar gel with holes, but still there is following deficiency in it: if when negative pressure is not enough, can not be disposable by the agar sucking-off in hole, need to again with syringe needle, choose, very easily easily pollute; And be by the slowly heating on flame of flat double dish, to make a little fusing of agar of hole feather edge, at the bottom of seal bore, complicated operation, is used inconvenience.
Therefore,, for above deficiency, the utility model provides a kind of poroid agar gel culture apparatus for immunodiffusion.
Utility model content
(1) technical matters that will solve
The purpose of this utility model is to provide a kind of poroid agar gel culture apparatus for immunodiffusion and makes the complicated operation existing, the problem that agar easily pollutes to solve poroid agar gel.
(2) technical scheme
In order to solve the problems of the technologies described above, the utility model provides a kind of poroid agar gel culture apparatus for immunodiffusion, the punching stake that it comprises ware body, ware lid and is fixedly connected on ware lid bottom surface, ware lid and ware body are interlocked, and the bottom surface of punching stake and the bottom surface of ware body have spacing.
Wherein, described punching stake is prism or is right cylinder.
Wherein, the xsect of described ware body and ware lid is circle or rectangle.
Wherein, multiple punching stakes are fixed on ware lid bottom surface and form plum blossom-shaped.
Wherein, the number of described punching stake is five or seven, and one of them punching stake is positioned at Min Gai center, and the punching stake at remaining punching Zhuan Raomingai center is evenly arranged.
Wherein, the sidewall of described ware lid is positioned at the sidewall outside of described ware body.
Wherein, the material of described ware body, ware lid and punching stake is glass.
Wherein, the spacing between every two punching stakes is 3mm.
(3) beneficial effect
Technique scheme tool of the present utility model has the following advantages: the poroid agar gel culture apparatus for immunodiffusion that the utility model provides is fixedly connected with punching stake in ware lid bottom surface, when ware lid and ware body closure, the bottom surface of punching stake and the bottom surface of ware body have spacing, the agar solution melting in ware body is stretched in punching stake after agar is naturally cooling, take out punching stake, in agar gel, formed the regular hole consistent with punching stake profile, this process operation is simple, and agar gel is also difficult for polluting.
Accompanying drawing explanation
Fig. 1 is the structural representation (wiring layout) of the poroid agar gel culture apparatus of the utility model embodiment;
Fig. 2 is the structural representation (upward view) of ware lid in the utility model embodiment;
Fig. 3 is the poroid agar gel culture apparatus of the utility model embodiment structural representation (cut-open view).
In figure, 1: ware lid; 2: ware body; 3: punching stake.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used for illustrating the utility model, but are not used for limiting scope of the present utility model.
As shown in figures 1 and 3, the poroid agar gel culture apparatus for immunodiffusion that the utility model provides, the punching stake 3 that it comprises ware body 2, ware lid 1 and is fixedly connected on ware lid 1 bottom surface, ware body 2 and ware lid 1 can snap together, when ware lid 1 and ware body 2 fasten, the bottom surface of punching stake 3 and the bottom surface of ware body 2 have spacing with the hole at the bottom of guaranteeing to form blind hole when making agar gel, and this spacing is preferably 1mm, and punching stake 3 is airtight cavity or is solid.Wherein, the xsect of ware body 2 and ware lid 1 is circular, and ware body 2 is flat, and punching stake 3 is right cylinder, can be hollow, can be also solid.
Certainly, the structure of ware body 2 and ware lid 1 can be also prism or other version; Punching stake 3 also can be prism structure; The material of ware body 2, ware lid 1 and punching stake 3 is preferably glass, can be also plastics; The height of ware lid 1 is 10mm.
Further, as shown in Figure 2, multiple punching stakes 3 are fixed on ware lid 1 bottom surface and form plum blossom-shaped; The number of punching stake 3 is preferably five or seven, and one of them punching stake 3 is positioned at the center of ware lid 1, and remaining is evenly arranged around the punching stake 3 at ware lid 1 center; The diameter of ware lid 1 is greater than the diameter of ware body 2, is also the sidewall outside that the sidewall of ware lid is positioned at ware body; Preferably, the spacing between every two punching stakes is 3mm.
The concrete implementation step of the poroid agar gel culture apparatus of the utility model is:
1, in the agar solution of thawing being poured in flat ware body 2, maintenance sample path length is 2.5mm～3mm;
2, then punching stake 3 is fixed on ware lid 1 bottom surface, covers on flat ware body 2, treat that agar is naturally cooling;
3, open ware lid 1, take off punching stake 3, form the poroid agar gel of Pass Test requirement.
In sum, the poroid agar gel culture apparatus for immunodiffusion that the utility model provides is fixedly connected with the punching stake 3 of airtight cavity or solid in ware lid 1 bottom surface, the bottom surface of punching stake 3 and the bottom surface of ware body 2 have spacing, like this when ware lid 1 and ware body 2 closure, the agar solution melting in ware body 2 is stretched in punching stake 3 after agar is naturally cooling, take out punching stake 3, in agar gel, formed the regular hole consistent with punching stake 3 profiles, this hole meets immunodiffusion requirement completely, this process operation is simple, agar gel is also difficult for polluting.
The above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; do not departing under the prerequisite of the utility model know-why; can also make some improvement and modification, these improve and modification also should be considered as protection domain of the present utility model.
Claims (8)
1. for a poroid agar gel culture apparatus for immunodiffusion, it is characterized in that: the punching stake that it comprises ware body, ware lid and is fixedly connected on ware lid bottom surface, ware lid and ware body are interlocked, and the bottom surface of punching stake and the bottom surface of ware body have spacing.
2. poroid agar gel culture apparatus according to claim 1, is characterized in that: described punching stake is prism or is right cylinder.
3. poroid agar gel culture apparatus according to claim 2, is characterized in that: the xsect of described ware body and ware lid is circle or rectangle.
4. according to the poroid agar gel culture apparatus described in claim 1-3 any one, it is characterized in that: multiple punching stakes are fixed on ware lid bottom surface and form plum blossom-shaped.
5. poroid agar gel culture apparatus according to claim 4, is characterized in that: the number of described punching stake is five or seven, and one of them punching stake is positioned at Min Gai center, and the punching stake at remaining punching Zhuan Raomingai center is evenly arranged.
6. poroid agar gel culture apparatus according to claim 3, is characterized in that: the sidewall of described ware lid is positioned at the sidewall outside of described ware body.
7. poroid agar gel culture apparatus according to claim 1, is characterized in that: the material of described ware body, ware lid and punching stake is glass.
8. poroid agar gel culture apparatus according to claim 5, is characterized in that: the spacing between every two punching stakes is 3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320727641.8U CN203561638U (en) | 2013-11-18 | 2013-11-18 | Hole-shaped agar gel culture apparatus for immunodiffusion test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320727641.8U CN203561638U (en) | 2013-11-18 | 2013-11-18 | Hole-shaped agar gel culture apparatus for immunodiffusion test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203561638U true CN203561638U (en) | 2014-04-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320727641.8U Expired - Fee Related CN203561638U (en) | 2013-11-18 | 2013-11-18 | Hole-shaped agar gel culture apparatus for immunodiffusion test |
Country Status (1)
| Country | Link |
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| CN (1) | CN203561638U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104816340A (en) * | 2015-05-08 | 2015-08-05 | 齐鲁工业大学 | Agar plate perforating device and application thereof |
| CN104819879A (en) * | 2015-04-30 | 2015-08-05 | 遵义医学院 | Multifunctional agar puncher |
| CN113303224A (en) * | 2021-06-24 | 2021-08-27 | 河北农业大学 | Experimental cotton seedling water culture device and method without damaging root system |
-
2013
- 2013-11-18 CN CN201320727641.8U patent/CN203561638U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104819879A (en) * | 2015-04-30 | 2015-08-05 | 遵义医学院 | Multifunctional agar puncher |
| CN104816340A (en) * | 2015-05-08 | 2015-08-05 | 齐鲁工业大学 | Agar plate perforating device and application thereof |
| CN104816340B (en) * | 2015-05-08 | 2017-04-26 | 齐鲁工业大学 | Agar plate perforating device and application thereof |
| CN113303224A (en) * | 2021-06-24 | 2021-08-27 | 河北农业大学 | Experimental cotton seedling water culture device and method without damaging root system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140423 Termination date: 20141118 |
|
| EXPY | Termination of patent right or utility model |