CN203535048U - Western blot chemical light emitting detection kit of allergen specific antibody IgE - Google Patents
Western blot chemical light emitting detection kit of allergen specific antibody IgE Download PDFInfo
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- CN203535048U CN203535048U CN201320566845.8U CN201320566845U CN203535048U CN 203535048 U CN203535048 U CN 203535048U CN 201320566845 U CN201320566845 U CN 201320566845U CN 203535048 U CN203535048 U CN 203535048U
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Abstract
The utility model discloses a western blot chemical light emitting detection kit of an allergen specific antibody IgE. The kit comprises a reaction slot, wherein a nylon transfer printing film bar is arranged at the bottom of the reaction tank and is wrapped with more than one allergen protein and more than one human IgE antibody standard product. The kit has the characteristics of high sensitivity, convenience in use and safety, and is high in accuracy.
Description
Technical field
The utility model belongs to immunology detection field, is specially a kind of Western blotting chemiluminescence detection kit of allergen specificity antibody IgE.
Background technology
Anaphylactia is modal a kind of disease anaphylactogen in modern society, and this is that the speed that mediated by allergen specificity antibody IgE is sent out parasexuality and reacted.Its ultimate principle is that body is subject to antigenic substance (also claiming allergen or anaphylactogen), and after the stimulations such as dust, pollen, food, parasite, the tissue damage causing or physiological function are disorderly.The Fc section of IgE molecule is with basophilic granulocyte and mast cell surface Fc receptors bind, make body in sensitization, when body is stimulated by similar anaphylactogen again, anaphylactogen makes IgE molecule crosslinked, cause the bioactivators such as basophilic granulocyte and mast cell degranulation release group glue, leukotriene, cell factor, allergic symptoms such as making body produce allergic reaction, cause body telangiectasis, oedema, smooth muscle spasm, endocrine are hyperfunction, as: pollinosis, hay fever (allergic rhinitis), allergic asthma, anaphylactic shock.
Allergic reaction clinically can be by Skin-test and various external detection method, as radioimmunoassay (RIA) method, Western blotting (WB), immunoprecipitation (IP) and Enzyme Linked Immunoadsorbent Assay (ELISA) method etc., in conjunction with disease symptom etc., determine anaphylactogen (the Sampson HA of sensitization, 1999, Food allergy, part2, J Al lergy Clin Immunol, 103:981-989).
Skin-test comprises intracutaneous test, pricking method test, scratch test, patch test, the provocative test of eye conjunctiva, schneiderian membrance provocative test and BPT etc.Intracutaneous test and pricking method test are the most frequently used, and the extract that contains a small amount of anaphylactogen is injected to intracutaneous, as the wheal that occurs similar bite by mosquitos is for irritated, generally can obtain testing result half an hour, has advantages of easy, quick, inexpensive.But false positive is high and be subject to the impact of medicine, as tested, in first 24 hours, taking Claritin can affect measurement result, has the patient of severe allergic reaction as made a skin test, and also has certain life danger.
Various external detection methods, by extracting a small amount of serum, detect the content of the specific IgE in sample, some anaphylactogen being produced, and complete the detection of anaphylactogen screening and specific allergen.Be particularly useful for cutaneous lesions and cannot do Skin-test person; Skin-test has certain dangerous person; Take the inaccurate person of Skin-test result after antiallergic.In the diagnosis of anaphylactogen disease, has promotional value.
Due to the pettiness extremely of the IgE antibody content in current blood of human body, there is under-sensitive weakness in the methods such as Western blotting (WB), immunoprecipitation (IP) and Enzyme Linked Immunoadsorbent Assay (ELISA) of generally using at present, and radioimmunology needs to be equipped with radioactive source, and there is certain risk.
Utility model content
The technical matters that the utility model solves is to provide a kind of Western blotting chemiluminescence detection kit of allergen specificity antibody IgE, to solve the problem proposing in above-mentioned background technology.
The technical matters that the utility model solves realizes by the following technical solutions:
A Western blotting chemiluminescence detection kit of allergen specificity antibody I gE, comprises reactive tank, and reaction tank bottom is provided with nylon transfer film bar; Described nylon transfer film bar is coated with more than one allergen proteins and more than one people IgE antibody standard substance.
Further, described reactive tank material is inertia PVC plastics.
Further, described coated allergen protein section is surveyed area.
Further, described coated people IgE antibody standard substance section is standard items region.
Beneficial effect
The utlity model has highly sensitive, easy feature fast and accurately, with traditional colorimetric Western blot comparison, sensitivity can improve an order of magnitude, can be effectively, examination anaphylactogen rapidly and sensitively.
Accompanying drawing explanation
Fig. 1 is the utility model structural representation.
Embodiment
In order to make of the present utility modelly to realize technological means, creation characteristic, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
As shown in Figure 1, a kind of Western blotting chemiluminescence detection kit of allergen specificity antibody IgE, comprises reactive tank 100, and reactive tank 100 bottoms are provided with nylon transfer film bar 200; Described nylon transfer film bar 200 is coated with more than one allergen proteins 211 and more than one people IgE antibody standard substance 221.
Further, described reactive tank 100 materials are inertia PVC plastics.
Further, 211 sections of described coated allergen proteins are surveyed area 210.
Further, 221 sections of described coated people IgE antibody standard substance are standard items region 220.
More than show and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that in above-described embodiment and instructions, describes just illustrates principle of the present utility model; do not departing under the prerequisite of the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.Claimed scope of the present utility model is defined by appending claims and equivalent thereof.
Claims (4)
1. a Western blotting chemiluminescence detection kit for allergen specificity antibody IgE, is characterized in that, comprises reactive tank, and reaction tank bottom is provided with nylon transfer film bar; Described nylon transfer film bar is coated with more than one allergen proteins and more than one people IgE antibody standard substance.
2. the Western blotting chemiluminescence detection kit of a kind of allergen specificity antibody IgE according to claim 1, is characterized in that, described reactive tank material is inertia PVC plastics.
3. the Western blotting chemiluminescence detection kit of a kind of allergen specificity antibody IgE according to claim 1, is characterized in that, described coated allergen protein section is surveyed area.
4. the Western blotting chemiluminescence detection kit of a kind of allergen specificity antibody IgE according to claim 1, is characterized in that, described coated people IgE antibody standard substance section is standard items region.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320566845.8U CN203535048U (en) | 2013-09-13 | 2013-09-13 | Western blot chemical light emitting detection kit of allergen specific antibody IgE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320566845.8U CN203535048U (en) | 2013-09-13 | 2013-09-13 | Western blot chemical light emitting detection kit of allergen specific antibody IgE |
Publications (1)
| Publication Number | Publication Date |
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| CN203535048U true CN203535048U (en) | 2014-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201320566845.8U Expired - Fee Related CN203535048U (en) | 2013-09-13 | 2013-09-13 | Western blot chemical light emitting detection kit of allergen specific antibody IgE |
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| Country | Link |
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| CN (1) | CN203535048U (en) |
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2013
- 2013-09-13 CN CN201320566845.8U patent/CN203535048U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140409 Termination date: 20150913 |
|
| EXPY | Termination of patent right or utility model |