CN203530312U - Detection diagnosis device for tumor cells or other pathological cells - Google Patents
Detection diagnosis device for tumor cells or other pathological cells Download PDFInfo
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- CN203530312U CN203530312U CN201320391678.8U CN201320391678U CN203530312U CN 203530312 U CN203530312 U CN 203530312U CN 201320391678 U CN201320391678 U CN 201320391678U CN 203530312 U CN203530312 U CN 203530312U
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Abstract
The utility model discloses a detection diagnosis device for tumor cells or other pathological cells. The detection diagnosis device comprises a laser source system for emitting laser with different wavelengths, a beam shaping system for shaping beam of the laser emitted from the laser source system, a laser confocal system for focusing focus points of shaped laser to the same horizontal plane, a light splitting system for the passing of the laser focused by the laser confocal system and the reflection of fluorescent light generated after laser irradiation of dyed cells, a microscope system for the passing of the laser emitted from the light splitting system and the amplification of the fluorescent light generated after the laser irradiation of the dyed cells, a cell flow system or a cell collection system for providing the dyed cells, a fluorescent light focusing system for receiving the reflected fluorescent light of the light splitting system, and a cell analysis system for analyzing and sorting the dyed cells to obtain a cell sorting result. The detection diagnosis device is high in detection speed and high in precision, and can be applied to early diagnosis, early treatment and prognostic judgment of the tumor cells and the other various pathological cells.
Description
Technical field
The utility model relates to diagnostic instrments, is specifically related to a kind of tumour cell or other pathological cells and detects diagnostic device.
Background technology
Capture cancer is the key subjects that medical circle is needed solution badly always, according to the WHO of World Health Organization, reports that in the recent period the global number of dying from every year cancer exceedes " 1,000 ten thousand is many ".Although people more and more pay close attention to the early diagnosis of tumour, 5 years survival rates of tumour patient are not still very desirable.By current medical level, to the early-stage cancer patient who does not occur to shift, approximately there is 80%-90% can cure above; But for the cancer patient in late period, the people after treatment more than energy 5-year Survival is just fewer.Its basic reason is exactly the means that lack really even super early discovery tumor tissues in early days and treat.Cancer patient, if discovery in time treatment in early days not only can improve survival rate, also can improve patient's life quality, so want early discovery, early diagnosis early treatment for cancer simultaneously.
Current cancer diagnosis and the method for research have: the methods such as the gene diagnosis of tumour, radio-immuno-image, molecular nuclear medicine technology, Diffusion-weighted imaging.
The gene diagnosis of tumour: the formation of tumour is the interactional result of inherited genetic factors and environmental factors, along with molecular biological, develop rapidly, people have developed into gene level to the understanding of tumour, many tumor-related genes have been found, and from gene level, cancer is diagnosed, also can judge histological good grade malignancy by detecting the gene marker relevant with canceration, or detect the progress of cancer.At present conventional gene diagnosis method can be summarized as three classes: nucleic acid gel electrophoresis, nucleic acid hybridization and polymerase chain reaction (PCR) technology and directly measure Disease-causing gene sequence, though gene diagnosis method may be found all genetic flaws or the corresponding disease that wherein exist, but because workload is excessive, cost is high, therefore be subject to certain limitation for clinical diagnosis at present.
Radio-immuno-image method: radio-immuno-image is the technology that a kind of antibody of antitumor and related antigen of take radioisotope labeling is developer positioning tumor.Primary process is: traget antibody directionally combines with tumour cell after vein or other approach are injected in vivo; In in due course, by radionuclide, make whole body or regional imaging identify the residing position of tumour and size.Yet for parenchyma, after radionuclide arrival tumor locus plays a role, tend to make healthy tissues to bear too much radiolesion.
Molecular nuclear medicine technology: in tumour generating process, due to sudden change and the amplification of gene, some acceptor on tumor cell membrane is overexpression usually.Utilize part and receptors bind to there is the feature of high specific, highly selective and high-affinity, adopt the part of radioisotope labeling as tracer agent, make the corresponding receptors bind of High Cell Density And High Expression on itself and tumour cell, thereby the technology that makes tumour be able to video picture is referred to as molecular core technology tumor imaging.The tracer technique of application nuclear medicine, disclose the variation of pathological tissues cell receptor, the unconventionality expression of gene, the abnormal change of biochemistry, metabolism and cell signalling etc., for the diagnosis of tumour, good pernicious discriminating, clinical stages, curative effect evaluation and prognosis detection etc. provide information, for tumor research, provide the foundation of molecular level simultaneously.Molecular core diagnosis of technique tumour is maximum technology of using at present, but molecular core technology is not very desirable to the early diagnosis effect of tumour cell, acts on limited and repeatedly carry out nuclear inspection patient's health is also had to certain infringement on early diagnosis of tumor.
Diffusion-weighted imaging method: Diffusion-weighted imaging is to utilize the special sequence of nuclear magnetic resonance (MRI) to observe a kind of formation method of the microcosmic disperse campaign of water molecules in biological tissue, it is a kind of imaging technique to water molecules disperse motion sensitive, with routine MRI T1 weighting picture in the past, T2 weighting picture is different, proton imaging (DWI) makes MRI be deep into more microscopic scale to the research of human body, can reflect the functional status of water molecules exchange between the spatial composing information of tissue and pathological and physiological condition Xia Ge morphological element.Follow the technical progress of mr software, the clinical application of DWI is also further extensive, from the central nervous system at initial stage, expands to each internal organs in body, and for tumour differential diagnosis provides new information, its effect has caused extensive concern.Yet most application, still in inquiring into conceptual phase, especially need further to further investigate the basis of disperse mechanism under condition of living body at present, and its clinical value also needs further excavation, be widely used in the clinical more experience of accumulation of still needing before.
Although the diagnosing tumor methods such as gene diagnosis, radio-immuno-image, molecular nuclear medicine technology, Diffusion-weighted imaging are all development to some extent at present, but these methods are mainly used in static qualitative analysis, can not at variation and the tumour cell of therapeutic process, shift by dynamic monitoring tumour cell, limited its fundamental research and clinical in application, especially in the needed accurate quantitative analysis analysis to cell number in diagnosis and therapeutic process of the major diseases such as malignant tumour.Therefore be necessary to develop new instrument and solve this problem, to meet the demand of cell number quantitative analysis in vivo.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, the early diagnosis of a kind of energy Real-Time Monitoring tumour cell variation and tumour cell transfer case at therapeutic process are provided, can carry out accurate quantitative analysis analysis to major disease needed cell number in diagnosis and therapeutic process, the cell gathering is carried out to tumour cell or other pathological cells detection diagnostic device that gene type, active somatic cell cultivation and drug susceptibility are identified.
To achieve these goals, the technical solution adopted in the utility model is:
The utility model provides a kind of tumour cell or other pathological cells to detect diagnostic device, utilize laser to irradiate tumour cell or other various pathological cells of dyeing, the fluorescent reflection by tumour cell or other various pathological cells judges that tumour or other various pathological cells send out metastatic cancer cell or the various pathological cells in circulation of blood; Simultaneously by cell collection to tumour cell carry out early diagnosis, personalized diagnosis, tumour carries out personalized treatment and prognosis judgement, tumour medicine sensitivity Detection, genetic screening and diagnosis, Diagnosis of Infectious Diseases, Immunological diseases are diagnosed, and expensive medication is treated the projects such as monitoring.
This tumour cell or other pathological cells detect diagnostic device, by the laser source system that sends different wave length laser, the laser that laser source system is sent carries out the beam shaping system of beam shaping, laser spot after shaping is converged to conplane laser co-focusing system, the laser converging for laser co-focusing system by and the beam splitting system of the fluorescence that produces after laser radiation of reflection staining cell, for the laser that sends from beam splitting system by and amplify the microscopic system of the fluorescence that staining cell produces after laser radiation, stream of cells system or the cell collection system of staining cell are provided, after receiving the fluorescence focusing system of beam splitting system reflected fluorescent light and analyzing staining cell, carry out cell sorting and show that the cell analysis system of cell sorting result forms.
Described laser source system can send one or more wavelength lasers in 405nm, 488nm, 514nm, 543nm, 561nm and 640nm, and the output rating of laser is greater than 5 milliwatts.
Described beam shaping system consists of plano-concave, plano-convex and average lens gummed.
Described laser co-focusing system consists of the achromatic lens gummed of different curvature, and achromatic lens is that achromatic lens is a kind of in plano-concave lens, plano-convex lens, average lens, plano-convex-plano-concave lens and plano-concave-plano-convex-average lens.
Described beam splitting system consists of half-reflection and half-transmission set of lenses gummed.
Described microscopic system is two lens object lens.
Described cell collection system comprises the blood flow pipeline that flows through of individual cells for dyeing, cell collection system acquisition cell and to the cell gathering dye, gene type, active somatic cell is cultivated and drug susceptibility is identified.
Described fluorescence focusing system consists of achromatic lens gummed, and achromatic lens is a kind of in plano-concave lens, plano-convex lens, average lens, plano-convex-plano-concave lens and plano-concave-plano-convex-average lens.
Described cell collection system comprises the blood flow pipeline flowing through for the individual cells dyeing.Described signal acquiring system is photoelectric commutator, and described Controlling System consists of controller and the display screen that is connected with controller.
The utility model has the advantage of: this tumour cell or other pathological cells detect diagnostic device, be the physics of cell (or particulate), physiology, pathology, biochemistry, immunity, heredity, molecular biology shape and functional status etc. to be carried out to a kind of modern cell analysis technology of qualitative and quantitative detection, there is following advantage:
1, can up to ten thousand cells of high speed analysis, and can from a cell, record a plurality of parameters simultaneously, compare with traditional fluoroscopy, there is the advantages such as speed is fast, precision is high, accuracy is good, can be applied in the early detection diagnosis of tumour cell and other various pathological cells.
2, can Real-Time Monitoring the existence of certain or some targeted cell population in body (animalcule or human body) circulation of blood, as the metastatic cancer cell in circulation of blood after solid tumor blood dissemination, and carry out quantitative analysis.
3, can accomplish that Chang Shi Cheng ﹑ monitors the labeled cell in the same live body recycle system continuously.
4, can dye to tumour cell, gene type, supravital cultivate and tumour medicine susceptibility is identified, and result for the treatment of and the prognosis of judgement tumour.
5, this equipment is of many uses: can be widely used in clinical medicine and the basic medical research fields such as immunology, hematology, oncology, cytobiology, cytogenetics, biological chemistry.
6, simple, the handled easily of this device structure, without wound, safe.
7, adopt liquid-crystal display, there is the advantages such as high, the digital interface of display quality, volume are little, lightweight and low in energy consumption.
8, realize instrument fully automated, need not be manual, there is very strong data processing and analysis ability, realize in real time and showing.
9, this equipment volume little, be easy to carry, disposable input life-time service, expense be low.
Accompanying drawing explanation
The content of below each width accompanying drawing of the utility model specification sheets being expressed and the mark in figure are briefly described:
Fig. 1 is the structural representation that the utility model tumour cell or other pathological cells detect diagnostic device;
Fig. 2 is the structural representation that Fig. 1 tumour cell or other pathological cells detect the beam shaping system of diagnostic device;
Fig. 3 is the structural representation of the unicellular blood flow pipeline of Fig. 1 tumour cell or other pathological cells cell collection system that detects diagnostic device;
Mark in above-mentioned figure is:
A, laser source system
B, laser beam shaping system
C, laser co-focusing system
D, beam splitting system
E, microscopic system
F, stream of cells system or cell collection system
G, fluorescence focusing system
H, signal acquiring system
L, cell sorting system
M, data presentation system
N, blood flow pipe
O, unicellular arrangement funnel
Embodiment
Contrast accompanying drawing below, by the description to optimum embodiment, embodiment of the present utility model is described in further detail.
As shown in Figure 1, this tumour cell or other pathological cells detect diagnostic device, by laser source system A(exciting light), laser beam shaping system B, laser co-focusing system C, beam splitting system D, microscopic system E, stream of cells system or cell collection system F, fluorescence focusing system G and signal acquiring system H, cell sorting system L and data presentation system M etc. partly form; Wherein signal acquiring system H, cell sorting system L and data presentation system M form cell analysis system; Signal acquiring system H is for can be converted to optical signal the photoelectric commutator of electrical signal, and cell sorting system L and data presentation system M form Controlling System, signal acquiring system H connection control system; The software that cell sorting system L adds Matlab compiling by controller forms, and data presentation system M is for connecting LCD or the light-emitting diode display of controller.
The laser of the different wave length being sent by lasing fluorescence origin system A carries out beam shaping through beam shaping system B, and circular light spot is shaped to strip hot spot, and the laser of different wave length enters laser co-focusing system C after shaping.Laser co-focusing system C converges to the laser spot of different wave length on same plane, then through beam splitting system D, enters microscopic system E.Laser is irradiated on flow at high speed in stream of cells system or cell collection system F or fixing staining cell after microscopic system E, and laser radiation produces fluorescence after on staining cell.Fluorescence enters beam splitting system D after microscopic system E, after beam splitting system D reflection, enters fluorescence focusing system G, after fluorescence focusing system G focuses on, enters signal acquiring system H.After signal acquiring system H gathers fluorescent signal, convert optical signal to electrical signal and enter cell sorting system L, cell sorting system L carries out sorting to cell, then separation results is presented to data presentation system M upper, according to separation results, diagnoses.
The laser of laser source system A can be: the different wavelength such as 405nm, 488nm, 514nm, 543nm, 561nm, 640nm, laser is sent by laser apparatus, the type of laser apparatus can be the types such as solid statelaser, semiconductor laser, gas laser, optical fiber laser and dye laser, and the power of Laser output is greater than 5 milliwatts.
The Laser Transmission of laser source system A is in beam shaping system B, and beam shaping system B carries out beam shaping to laser, and circular light spot is shaped to long strip shape hot spot.Beam shaping system B consists of plano-concave, plano-convex and average lens gummed.
The lens that preferably form beam shaping system B are five groups.
As shown in Figure 2, as preferred implementation 1, beam shaping system B is arranged in order rear gummed by plano-convex, plano-concave, average, plano-convex and five groups of lens of plano-concave and forms.
As preferred implementation 2, beam shaping system B is arranged in order rear gummed by average, plano-convex, plano-concave, plano-convex and five groups of lens of plano-concave and forms.
As preferred implementation 3, beam shaping system B is arranged in order rear gummed by plano-convex, plano-concave, plano-convex, plano-concave and average five groups of lens and forms.
Laser after beam shaping system B shaping enters laser co-focusing system C, laser co-focusing system C by the laser focusing of different wave length to same plane.Laser co-focusing system C is formed by the achromatic lens gummed of different curvature, achromatic lens is a kind of in two lens gummeds, three lens gummeds, four lens gummeds and five lens gummed, and concave surface and the convex surface of two adjacent groups plano-concave balsaming lens and plano-convex balsaming lens cooperatively interact.
The curvature value of two lens gummeds, three lens gummeds, four lens gummeds and five lens gummed can from 15 to 50, and the concave surface of adjacent balsaming lens and convex curvature value add up to 0, i.e. the positive and negative correspondence of the concave surface of balsaming lens and convex surface.
Preferably light beam orthopedic systems B consists of five groups of achromatic lens gummeds.
As preferred embodiment 1, laser co-focusing system C is arranged in order gummed by plano-concave lens, plano-convex lens, average, plano-convex-plano-concave and plano-concave-plano-convex-average lens and forms.
As preferred embodiment 2, laser co-focusing system C is arranged in order gummed by plano-convex lens, plano-concave lens, average, plano-convex-plano-concave and plano-concave-plano-convex-average lens and forms.
As preferred embodiment 3, laser co-focusing system C is arranged in order gummed and is formed by plano-concave-plano-convex-average lens, plano-concave lens, plano-convex lens, average, plano-convex-plano-concave.
The curvature value of balsaming lens can be one or more in 15 to 50, and for example the concave curvature of plano-concave lens is R=-25, and planar curvature is 0; Convex curvature in plano-convex lens is R=25, and planar curvature is 0; Plano-convex-plano-concave and plano-concave-plano-convex-average lens convex curvature is R=30, and planar curvature is 0, and concave curvature is R=-30.
The staining cell that the laser of exporting from laser co-focusing system C enters stream of cells system (or cell collection system) F flow through beam splitting system D, microscopic system E irradiates.
Beam splitting system D is arranged in order gummed by half-reflection and half-transmission lens to form, and as preferred embodiment, beam splitting system D is arranged in order rear gummed by three groups of half-reflection and half-transmission lens and forms.Beam splitting system D is full impregnated to laser, and laser can not have lossy craspedodrome to pass through, and the fluorescence sending from staining cell reflexes in fluorescence focusing system G while passing through beam splitting system D.
Microscopic system E is two lens object lens, and it is to not effect of laser, and laser can directly pass through, and it can amplify the fluorescence reflecting from staining cell, is convenient to follow-up collection.
Stream of cells system F can adopt artery or the vein blood vessel of animalcule, human body, also can adopt cell collection system to substitute, and cell collection system acquisition human body or animalcule venous blood, then obtain the blood flow pipeline that individual cells flows through.As shown in Figure 3, blood flow pipeline consists of blood flow pipe N and the unicellular arrangement funnel O that is sleeved on outside blood flow pipe N; The blood that human body or animalcule vein are got is put into blood flow pipe, then through unicellular arrangement funnel O, forms unicellular blood flow pipeline.Blood flow pipeline can adopt transparent, the stable material such as opticglass, quartz to make.
Stream of cells system F or cell collection system, can dye cell, and to staining cell, produce afterwards fluorescence by laser radiation; Meanwhile, the cell of collection also can by dyeing, gene type, active somatic cell cultivate and cell to drug susceptibility evaluation etc.
The fluorescence that enters fluorescence focusing system G focuses in system, and it focuses on focus on the focal plane of signal acquiring system H, and the two forms conjugation.Fluorescence focusing system F consists of the achromatic lens gummed of different curvature equally, and the achromatic lens of different curvature can adopt respectively identical or different material to make, and all adopts bonding technique.In the achromatic lens of different curvature, every group of achromatic lens is a kind of in plano-concave lens, plano-convex lens, average lens, plano-convex-plano-concave lens and plano-concave-plano-convex-average lens, and concave surface and the convex surface of two adjacent groups plano-concave lens and plano-convex lens cooperatively interact.
The curvature value of plano-concave lens, plano-convex lens, average lens, plano-convex-plano-concave lens and plano-concave-plano-convex-average lens can from 15 to 50, and the concave surface of adjacent balsaming lens and convex curvature value add up to 0, i.e. the positive and negative correspondence of the concave surface of balsaming lens and convex surface.
Fluorescence focusing system G is identical with laser co-focusing system C set of lenses structure, but the making material of set of lenses is different, due to fluorescence focusing system G light strength ratio a little less than, need to select the few material of fluorescent absorption is made as far as possible, preferably fluorescence focusing system G adopts and melts quartz material.And laser co-focusing system material can be selected common K9 glass, also can select and melt quartz.
Preferably fluorescence focusing system G consists of five groups of achromatic lens gummeds.
As preferred embodiment one, fluorescence focusing system G is arranged in order gummed by plano-concave lens, plano-convex lens, average, plano-convex-plano-concave and plano-concave-plano-convex-average lens and forms.
As preferred embodiment 2, fluorescence focusing system G is arranged in order gummed by plano-convex lens, plano-concave lens, average, plano-convex-plano-concave and plano-concave-plano-convex-average lens and forms.
As preferred embodiment 3, fluorescence focusing system G is arranged in order gummed by plano-concave-plano-convex-average lens, plano-concave lens, plano-convex lens, average and plano-convex-plano-concave and forms.
The curvature value of balsaming lens can be one or more in 15 to 50, and for example the concave curvature of plano-concave lens is R=-25, and planar curvature is 0; Convex curvature in plano-convex lens is R=25, and planar curvature is 0; Plano-convex-plano-concave and plano-concave-plano-convex-average lens convex curvature is R=30, and planar curvature is 0, and concave curvature is R=-30.
Signal acquiring system H is photoelectric commutator, converts optical signal to electrical signal, and signal acquiring system H is preferably photomultiplier.
The signal collecting through signal acquiring system H is sent to cell sorting system L, cell sorting system L processes and sorting the cell signal collecting, finally obtain the quantity with the cell of fluorescent signal, and the data of analyzing are out sent on data presentation system M; Data presentation system M indicating system data, for science and clinical study, only have tumour cell or other pathological cells just to have fluorescent signal, with this, draw diagnostic result.
Utility model people has made a model machine, and this model machine Optical Maser System A adopts the semiconductor laser that wavelength is 488nm, and power is 50mw.Beam shaping system B is arranged in order rear gummed by five groups of plano-convexs, plano-concave, average, plano-convex and five groups of lens of plano-concave and forms.
Laser co-focusing system C and fluorescence focusing system G adopt respectively plano-concave lens, plano-convex lens, average, plano-convex-plano-concave and plano-concave-plano-convex-average lens to be arranged in order gummed and form.
Laser co-focusing system C selects K9 glass to make, and fluorescence focusing system G adopts and melts quartz material.
Microscopic system E adopts a two lens microcobjective, and stream of cells system F adopts mouselet ear vein.
Signal acquiring system H is photoelectric commutator, and signal acquiring system H connects computer, and cell sorting system L selects the software of Matlab compiling, and this software moves on computers, realizes the sorting of cell; Whole system by computer control unit control, data are presented on indicating meter in real time.
This tumour cell or other pathological cells detect diagnostic device, are the physics of cell (or particulate), physiology, pathology, biochemistry, immunity, heredity, molecular biology shape and functional status etc. to be carried out to a kind of modern cell analysis technology of qualitative and quantitative detection.It can up to ten thousand cells of high speed analysis, and can from a cell, record a plurality of parameters simultaneously, compare with traditional fluoroscopy, have the advantages such as speed is fast, precision is high, accuracy is good; Be contemporary state-of-the-art cell quantitative technology, can be used for leukemic somatotype, the chromosomal heteroploidy mensuration of tumour cell and immunology research, the counting of the identification of Bacteria Identification, virus infected cell and AIDS-infected T4, T8 cell.Can be widely used in clinical medicine and the basic medical research fields such as immunology, hematology, oncology, cytobiology, cytogenetics, biological chemistry.
In existence that can Real-Time Monitoring certain (some) targeted cell population in body (animalcule or human body) circulation of blood aspect lesion detection, as the metastatic cancer cell in circulation of blood after solid tumor blood dissemination, and carry out quantitative analysis; Can carry out automatic monitor for continuously to the wandering cells in (animalcule or human body) recycle system and be uploaded to the storage system of equipment.
This tumour cell or other pathological cells detect diagnostic device, with high energy laser, irradiate under high-speed motion state by the unicellular or particulate of fluorescent staining, measure the intensity of scattered light and the emitting fluorescence of its generation, thereby the physics of cell (or particulate), physiology, pathology, biochemistry, immunity, heredity, molecular biology shape and functional status etc. are carried out to qualitative and quantitative detection, its set electron technology, computer technology, laser technology, channel theory, in one, are a kind of FA detecting instruments.
Above the utility model is exemplarily described; obviously the utility model specific implementation is not subject to the restrictions described above; as long as adopted the improvement of the various unsubstantialities that method of the present utility model design and technical scheme carry out; or without improving, design of the present utility model and technical scheme are directly applied to other occasion, all within protection domain of the present utility model.
Claims (9)
1. tumour cell or other pathological cells detect diagnostic device, it is characterized in that: by the laser source system that sends different wave length laser, the laser that laser source system is sent carries out the beam shaping system of beam shaping, laser spot after shaping is converged to conplane laser co-focusing system, the laser converging for laser co-focusing system by and the beam splitting system of the fluorescence that produces after laser radiation of reflection staining cell, for the laser that sends from beam splitting system by and amplify the microscopic system of the fluorescence that staining cell produces after laser radiation, stream of cells system or the cell collection system of staining cell are provided, after receiving the fluorescence focusing system of beam splitting system reflected fluorescent light and analyzing staining cell, carry out cell sorting and show that the cell analysis system of cell sorting result forms.
2. tumour cell as claimed in claim 1 or other pathological cells detect diagnostic device, it is characterized in that: described beam shaping system consists of plano-concave, plano-convex and average lens gummed.
3. tumour cell as claimed in claim 1 or 2 or other pathological cells detect diagnostic device, it is characterized in that: described laser co-focusing system consists of the achromatic lens gummed of different curvature, achromatic lens is a kind of in plano-concave lens, plano-convex lens, average lens, plano-convex-plano-concave lens and plano-concave-plano-convex-average lens.
4. tumour cell as claimed in claim 3 or other pathological cells detect diagnostic device, it is characterized in that: described beam splitting system consists of half-reflection and half-transmission set of lenses gummed.
5. tumour cell as claimed in claim 4 or other pathological cells detect diagnostic device, it is characterized in that: described microscopic system is two lens object lens.
6. tumour cell as claimed in claim 5 or other pathological cells detect diagnostic device, it is characterized in that: described cell collection system comprises the blood flow pipeline flowing through for the individual cells dyeing.
7. tumour cell as claimed in claim 6 or other pathological cells detect diagnostic device, it is characterized in that: described fluorescence focusing system consists of achromatic lens gummed, achromatic lens is a kind of in plano-concave lens, plano-convex lens, average lens, plano-convex-plano-concave lens and plano-concave-plano-convex-average lens.
8. tumour cell as claimed in claim 7 or other pathological cells detect diagnostic device, it is characterized in that: described cell analysis system, the Controlling System of being carried out sorting and demonstration by the cell information that optical signal is converted to the signal acquiring system of electrical signal and signal acquiring system is collected forms.
9. tumour cell as claimed in claim 8 or other pathological cells detect diagnostic device, it is characterized in that: described signal acquiring system is photoelectric commutator, and described Controlling System consists of controller and the display screen that is connected with controller.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320391678.8U CN203530312U (en) | 2013-07-02 | 2013-07-02 | Detection diagnosis device for tumor cells or other pathological cells |
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| CN201320391678.8U CN203530312U (en) | 2013-07-02 | 2013-07-02 | Detection diagnosis device for tumor cells or other pathological cells |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105352875A (en) * | 2015-09-28 | 2016-02-24 | 周辉 | Unmarked tumor cell detecting and diagnosing device and detecting and diagnosing method thereof |
| CN107692968A (en) * | 2017-09-26 | 2018-02-16 | 李铭 | A kind of tumor detection devices |
| CN114216850A (en) * | 2021-11-04 | 2022-03-22 | 万贝医疗健康科技(上海)有限公司 | Lung cancer cell activity state assessment equipment after radiotherapy and chemotherapy |
| WO2022166563A1 (en) * | 2021-02-04 | 2022-08-11 | 上海交通大学 | Apparatus for monitoring 2-nbdg-marked in vivo circulating tumor cells |
-
2013
- 2013-07-02 CN CN201320391678.8U patent/CN203530312U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105352875A (en) * | 2015-09-28 | 2016-02-24 | 周辉 | Unmarked tumor cell detecting and diagnosing device and detecting and diagnosing method thereof |
| CN107692968A (en) * | 2017-09-26 | 2018-02-16 | 李铭 | A kind of tumor detection devices |
| WO2022166563A1 (en) * | 2021-02-04 | 2022-08-11 | 上海交通大学 | Apparatus for monitoring 2-nbdg-marked in vivo circulating tumor cells |
| CN114216850A (en) * | 2021-11-04 | 2022-03-22 | 万贝医疗健康科技(上海)有限公司 | Lung cancer cell activity state assessment equipment after radiotherapy and chemotherapy |
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