CN203508047U - Paper-based microfluid device capable of automatically switching channels and biochemical diction device made by paper-based microfluid device - Google Patents
Paper-based microfluid device capable of automatically switching channels and biochemical diction device made by paper-based microfluid device Download PDFInfo
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- CN203508047U CN203508047U CN201320518179.0U CN201320518179U CN203508047U CN 203508047 U CN203508047 U CN 203508047U CN 201320518179 U CN201320518179 U CN 201320518179U CN 203508047 U CN203508047 U CN 203508047U
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Abstract
The utility model discloses a paper-based microfluid device capable of automatically switching channels and a biochemical diction device made by the paper-based microfluid device. The paper-based microfluid device comprises a plurality of overlapped paper-based microfluid monolayers which respectively contain a hydrophobic isolation area and a hydrophilic microfluidic channel to form microfluidic channels, and microfluidic switches are arranged on the microfluidic channels; water-absorbent resins are arranged between the microfluidic channels and the microfluidic switches, after the fluid flows into the water-absorbent resins, the water-absorbent resins expand to push the microfluidic switches and reagent carrying liners arranged under the microfluidic switches, and after the reagent carrying liners move to be contacted with the microfluidic channels arranged under the reagent carrying liners, the other microfluidic channel is opened, so that the channels can be automatically switched. After the device is adopted, the function that the paper-based microfluid automatically switches the channels can be realized, various biochemical reagents are introduced by automatic switching of the channels to carry out the multi-step reaction in the case that any auxiliary equipment and professionals are not needed, the device is taken as a part of the biochemical diction device, and the biochemical diction device is microminiaturized, so that the paper-based microfluid device has the function of amplifying informational molecules to be detected.
Description
Technical field
The utility model relates to a kind of paper substrate microfluidic device, is specifically related to a kind of paper substrate microfluidic device of the passage that automatically switches and the biochemical detection device of being made by it.
Background technology
Volume is little, amount of samples is few because having for micro-fluidic chip, operation is relatively simple and analytic function is powerful, become the study hotspot of biochemical analysis field, in the various fields such as DNA sequencing, crystallization of protein, single cell analysis, single molecule analysis, medical diagnosis on disease and food security, applied.And paper substrate micro-fluidic chip is as the microfluidic device of the brief version of ＂ ＂, compare with micro-fluidic chip, because of its have with low cost, be easy to carry, simple to operate, without remarkable advantages such as any attachment device and professionals, caused widely and paid attention to.
Paper substrate microfluidic device is (as sprayed the various ways such as wax or other hydrophobic substances on paper) after paper is processed, form hydrophilic microchannel and hydrophobic isolated area, hydrophobic isolated area is as passage barrier, hydrophilic microchannel utilizes the capillary force of paper fibre (element) to realize transporting of miniflow, without extraneous driving force.
Up to the present, the flow-guiding channel in existing paper substrate microfluidic device is all fixed, therefore in the situation that there is no auxiliary equipment, be difficult to the trend of microfluid in control channel.When existing paper substrate microfluidic device is used for biochemical analysis field, use existing paper substrate microfluidic device to carry out biochemistry detection and can only complete simple biochemical reaction step, function singleness, the biochemical reaction that but cannot complete multi-step further detects.
And most biochemical reaction is the continuous process of multi-step, multi-layer, differential responses time, and the detection sensitivity that the biochemical reaction of multi-step can reach exceeds much than single biochemical reaction.For example, comparatively the sensitivity of the effluent test strips colloid gold label one-step method of extensive use is approximately every milliliter 10 at present
-9gram (ng/ml), and adopt plasma EUSA (Plasmonic ELISA) double antibody sandwich method of multi-step, hypersensitization can reach every milliliter 10 to the sensitivity of antigen
-18gram (ag/ml).In the situation that specificity is close, sensitivity obviously improves.Therefore while carrying out biochemical detection with existing paper substrate microfluidic device, because it cannot complete multi-step biochemical reaction, make its detection sensitivity limited, be subject to certain limitation in application.
Utility model content
The problem existing for prior art, the utility model provides a kind of paper substrate microfluidic device of the passage that automatically switches.Simultaneously, the utility model also further provides the biochemical detection device of this paper substrate microfluidic device of application, by this device, without any need for auxiliary equipment and professional in the situation that, can complete multi-step, multi-layer, the biochemical reaction of differential responses time and carry out sample detection.
For achieving the above object, the paper substrate microfluidic device of the utility model automatic switchover passage, comprise some stacked paper substrate microfluid individual layers that contain hydrophobic isolated area and hydrophilic microchannel that add, form some biochemical reaction microchannels and some resins are subject to water microchannel, microfluid bottom is provided with miniflow output; Microchannel on microfluid individual layer is provided with flap valve that three disconnections, one side are connected with hydrophobic isolated area as microfluidic switch together with the hydrophobic isolated area of microchannel correspondence position on its upper and lower two layers of paper based microfluid individual layer, in microfluidic switch, being provided with reagent carrying liner resin is subject to be provided with water-absorbing resin between water microchannel and microfluidic switch, water-absorbing resin is provided with corresponding water-absorbing resin expansion space, and microfluidic switch is provided with corresponding microfluidic switch activity space; Liquid is subject to water microchannel to flow into water-absorbing resin through resin, resin imbibition promotes microfluidic switch and reagent carrying liner downwards, reagent carrying liner move down with biochemical reaction microchannel be thereunder set contact after, open another microchannel and complete passage and automatically switch.
Further, be provided with described water-absorbing resin expansion space between described water-absorbing resin and described microfluidic switch, expanding in the absorb water described water-absorbing resin expansion space of backward below of described water-absorbing resin, promotes described microfluidic switch and open; Described microfluidic switch below is provided with described microfluidic switch activity space, and when described microfluidic switch starts, the described reagent carrying liner being arranged in described microfluidic switch is moved downward to described microfluidic switch activity space.
Further, described microfluidic switch controls separately biochemical reaction microchannel or resin is subject to the break-make of water microchannel, or controls biochemical reaction microchannel simultaneously and provide the resin of water to be subject to the break-make of water microchannel for the water-absorbing resin of described microfluidic switch top.
Further, described device is subject to the width of water microchannel and length to control the described microfluidic switch opening time by controlling described resin.
Further, when described reagent carrying liner is when arranging that thereunder described biochemical reaction microchannel contacts, described reagent carrying liner is tandem with two biochemical reaction microchannels below it and is connected.
Further, described miniflow output is positioned at top layer or the bottom of described paper substrate microfluidic device.
Further, described water-absorbing resin be dome-type ball piece, rectangle, square or other there is the special-shaped water-absorbing resin that is subject to horizontal plane.
Further, described microfluid individual layer consists of filter paper, is provided with wax layer and forms described hydrophobic isolated area on filter paper.
A biochemical detection device that utilizes the paper substrate microfluidic device of above-mentioned automatic switchover passage to detect, comprises matrix, and top, matrix one end is provided with paper substrate microfluidic device as buffer solution applied area; The middle part of matrix is provided with some parallel effluent test strips, and the miniflow output of paper substrate microfluidic device extends the port separating with effluent test strips equal number, and with the corresponding connection in one end of each effluent test strips; The miniflow output extending is as testing sample applied area, and it is arranged with crosslinked liner; Matrix other end top is provided with water absorption cushion, partly overlaps with effluent test strips; Wherein, the crosslinked liner under reagent in paper substrate microfluidic device carrying liner and testing sample applied area, and be arranged on detection line in effluent test strips and control line and contain as required different biochemical reagents, for biochemical reaction, detect and analysis.
Further, described water absorption cushion consists of water-absorption fiber, and the part overlapping with described effluent test strips is arranged on described effluent test strips top; Described effluent test strips material is nitrocellulose membrane, PVDF membrane (PVDF membrane) or nylon membrane (nylon membrane).
The paper substrate microfluidic device of automatic switchover passage of the present utility model has been realized the function of automatic switchover passage, can introduce multiple biochemical reagents by the automatic switchover of passage, carry out multi-step biochemical reaction, make paper substrate microfluidic device possess the function of amplifying detected informational molecule.It can be used as the part in biochemical detection device for biochemistry detection analysis field, designs the biochemical detection device with the paper substrate microfluid of automatic switchover passage, has the following advantages.
This biochemical detection device have cheapness, portable, quick, sensitive, special, micro-, simple to operate, need not any auxiliary equipment and the advantage such as professional, be the biochemical detection device that a kind of cost performance is high.
Biochemical detection device microminiaturization of the present utility model carry out the checkout gear of biochemical reaction, without any need for auxiliary equipment and professional in the situation that, can carry out multi-step biochemical reaction, reduced manual operation error, convenient enforcement detected in real time, is particularly suitable for the occasion at resource-constraineds such as the family that does not possess auxiliary equipment and professional, rural area, pasture, field and disasters.
3. biochemical detection device of the present utility model is used full paper substrate structure, with low cost, after use, can destroy by fire, prevents harmful sample contaminated environment, energy-conserving and environment-protective.
4. biochemical detection device of the present utility model adopts modular combination, can select corresponding paper substrate microfluidic device, crosslinked liner and effluent test strips to combine according to different testing goals.
Accompanying drawing explanation
Fig. 1 is the layering schematic diagram of the paper substrate microfluidic device of embodiment;
Fig. 2 is the front view of the utility model biochemical detection device;
Fig. 3 is the upward view of paper substrate microfluidic device bottom and bottom extension area in embodiment;
Fig. 4 is the operational flowchart of embodiment mesophytization checkout gear;
Wherein, 1 is buffer solution applied area, and 2 is testing sample applied area, and 3 is matrix, and 4 is effluent test strips, and 5 is detection line, and 6 is control line, and 7 is water absorption cushion, and J is paper substrate microfluidic device bottom.
The specific embodiment
Below, with reference to accompanying drawing 1-4, the utility model is more fully illustrated, shown in the drawings of exemplary embodiment of the present utility model.Yet the utility model can be presented as multiple multi-form, and should not be construed as the exemplary embodiment that is confined to narrate here.But, these embodiment are provided, thereby make the utility model comprehensively with complete, and scope of the present utility model is fully conveyed to those of ordinary skill in the art.
For ease of explanation, here can use such as " on ", the space relative terms such as D score " left side " " right side ", the relation for element shown in key diagram or feature with respect to another element or feature.It should be understood that except the orientation shown in figure, spatial terminology is intended to comprise the different azimuth of device in using or operating.For example, if the device in figure is squeezed, be stated as the element that is positioned at other elements or feature D score will be positioned at other elements or feature " on ".Therefore, exemplary term D score can comprise upper and lower orientation both.Device can otherwise be located (90-degree rotation or be positioned at other orientation), and the relative explanation in space used here can correspondingly be explained.
Embodiment
Use plasma EUSA (Plasmonic ELISA) double antibody sandwich method to carry out sample detection, the paper substrate microfluidic device of preparation automatic switchover passage and corresponding biochemical detection device.The paper substrate microfluidic device hierarchical diagram of automatic switchover passage as shown in Figure 1.
The paper substrate microfluidic device of the automatic switchover passage of the present embodiment is comprised of microfluid individual layer A layer, microfluid individual layer B layer, microfluid individual layer C layer, microfluid individual layer D layer, microfluid individual layer E layer, microfluid individual layer F layer, microfluid individual layer G layer, microfluid individual layer H layer, microfluid individual layer I layer, ten layers of individual layer stack of microfluid individual layer J layer, and every layer of individual layer prepared hydrophobic isolated area and hydrophilic microchannel by spray wax on filter paper.After stack, form biochemical reaction microchannel and resin and be subject to water microchannel, as follows:
Resin is subject to water microchannel A:A1-B1-C1-D1-E1-F1-F2-E2-D2-C2;
Resin is subject to water microchannel B:A1-B1-C1-D1-E1-F1-F3-E3-D3-C3;
Biochemical reaction microchannel A:A4-B4-C4-D4-E4-F4-G4-H4-I4-J4;
Biochemical reaction microchannel B:A5-B5-C5-D5-E5-F5-G5-H5-I5-J5;
Biochemical reaction microchannel C:A1-B1-C1-D1-E1-F1-G1-H1-I1-J1.
Microchannel J4 in miniflow bottom J layer finally flows out the passage of paper substrate microfluid as miniflow, be provided with J8 miniflow output.
Resin on microfluid individual layer F layer is subject on water microchannel A and biochemical reaction microchannel A, together with the hydrophobic isolated area of its upper and lower corresponding position on the upper strata microfluid individual layer G of the microfluid individual layer E Ceng He lower floor layer of microfluid individual layer F layer, be provided with flap valve that three disconnections, one side are connected with isolated area as microfluidic switch A, be that equal-sized flap valve E6, flap valve F6, flap valve G6 form microfluidic switch A jointly, and control the break-make that resin is subject to water microchannel A and biochemical reaction microchannel A simultaneously.Equally, microfluidic switch B consists of jointly equal-sized flap valve E7, flap valve F7, flap valve G7, controls the break-make that resin is subject to water microchannel B and biochemical reaction microchannel B simultaneously.
At resin, be subject to be provided with dome-type water-absorbing resin piece C6 between the part microchannel C2 of water microchannel A and microfluidic switch A, its corresponding below is provided with imbibition space D6.Equally, at resin, be subject to be provided with dome-type water-absorbing resin piece C7 between the part microchannel C3 of water microchannel B and microfluidic switch B, its corresponding below is provided with imbibition space D7.
On microfluid individual layer H layer, microfluidic switch A below is provided with reagent carrying liner H6, and reagent carrying liner H6 below is provided with the microfluidic switch activity space I6 of microfluidic switch A.Equally, microfluidic switch B below is provided with reagent carrying liner H7, and reagent carrying liner H7 below is provided with the microfluidic switch activity space I7 of microfluidic switch B.
The present embodiment is subject to the width of water microchannel and length to control the microfluidic switch opening time by controlling resin.When the buffer solution to paper substrate microfluidic device applies the A8 of place dropping buffer solution, buffer solution flows and starts to be subject to water microchannel A and resin flowed by water microchannel B to biochemical reaction microchannel A, biochemical reaction microchannel B, biochemical reaction microchannel C, resin along hydrophilic microchannel simultaneously.First through biochemical reaction microchannel A by miniflow output J8 output microfluid, and biochemical reaction microchannel B and biochemical reaction microchannel C stop after flowing to respectively the microchannel J1 of microfluid bottom J layer and microchannel J5.
Until buffer solution, through biochemical reaction microchannel A, flow out microfluid after a period of time, the buffer solution resin of flowing through is subject to arrive microchannel C2 and flow into water-absorbing resin C6 after water microchannel A, water-absorbing resin C6 water suction is expanded and is promoted microfluidic switch A to D6 place, imbibition space, and first microfluidic switch A opens.Along with opening, microfluidic switch A moves down, microchannel A disconnects, and reagent carrying liner H6 moves down the microfluidic switch activity space I6 place of the microfluidic switch A that arrives microfluid individual layer I layer thereupon, reagent carrying liner H6 two ends contact with microchannel J4 and microchannel J5 respectively, microchannel J4 and J5 are connected to the new biochemical reaction microchannel B ' of formation (A5-B5-C5-D5-E5-F5-G5-H5-I5-J5-H6-J4), now buffer solution flows out microfluid through biochemical reaction microchannel B ', and microfluid completes passage first and automatically switches.
Until buffer solution, through biochemical reaction microchannel B ', flow out microfluid after a period of time, the buffer solution resin of flowing through is subject to arrive microchannel C3 and flow into water-absorbing resin C7 after water microchannel B, water-absorbing resin C7 water suction is expanded to D7 place, imbibition space and is promoted microfluidic switch B, and microfluidic switch B opens.Along with opening, microfluidic switch B moves down, microchannel B ' disconnects, and reagent carrying liner H7 moves down the microfluidic switch activity space I7 place of the microfluidic switch B that arrives microfluid individual layer I layer thereupon, reagent carrying liner H7 two ends contact with microchannel J4 and microchannel J1 respectively, microchannel J4 and J1 are connected to the new biochemical reaction microchannel C ' of formation (A1-B1-C1-D1-E1-F1-G1-H1-I1-J1-H7-J4), now buffer solution flows out microfluid through biochemical reaction microchannel C ', and microfluid completes passage for the second time and automatically switches.
Use the paper substrate microfluid of said structure as buffer solution applied area, form the biochemical detection device for plasma EUSA double antibody sandwich method analyzing and testing method, device front view is as shown in 2, and Fig. 3 is the upward view of paper substrate microfluidic device bottom and bottom extension area.This biochemical detection device comprises matrix 3, and the top, one end of matrix 3 is provided with paper substrate microfluidic device as buffer solution applied area 1.The middle part of matrix 3 is provided with two parallel effluent test strips 4, is respectively arranged with detection line 5 and control line 6 in effluent test strips 4.The miniflow output J8 of paper substrate microfluidic device bottom J layer extend and separate two miniflow output port J9 respectively with the corresponding connection in one end of two effluent test strips 4; The miniflow output extending is as testing sample applied area 2, and it is arranged with crosslinked liner (not shown).Matrix 3 other end tops are provided with water absorption cushion 7, partly overlap with effluent test strips 4.
Wherein, in reagent carrying liner H6 in paper substrate microfluidic device, contain enzyme mark SA, in reagent carrying liner H7, contain colour reagent, in crosslinked liner under testing sample applied area 2, contain specific antibody, in effluent test strips, contain the specific antibody being fixed on detection line 5 and the antibody that is fixed on the desmoenzyme mark SA on control line 6, control line 6 is used as quality testing.
When dripping the sample that contains antigen in testing sample applied area, plasma EUSA double antibody sandwich method detection method operating process as shown in Figure 4.First fluid sample is applied to testing sample applied area 2, and the antigen in testing sample combines with the antibody in the crosslinked liner below of testing sample applied area 2.Meanwhile, buffer solution is applied on buffer solution applied area 1, buffer solution flow to place, testing sample applied area along paper substrate microfluid mesophytization reaction microchannel A, and antigen and antibody conjugates flow to effluent test strips 4 under buffer solution flushing action; Along with buffer solution continuously outflows flushing, antigen and antibody conjugates arrive detection line 5 places of effluent test strips 4, be fixed on antibody capture antigen on detection line 5 and the bond of antibody, the bond of not captive antigen and antibody is cushioned liquid and rinses to water absorption cushion 7.
After the remnant of the antigen in effluent test strips 4 and antibody conjugates is rinsed well, paper substrate microfluid microfluidic switch A opens, biochemical reaction microchannel A disconnects, reagent carrying liner H6 on microfluidic switch A touches microchannel J4 and microchannel J5, biochemical reaction channel B ' open, and the enzyme mark SA that reagent is carried in liner H6 discharges, enzyme mark SA is along with buffer solution is by biochemical reaction channel B ' flow to effluent test strips 4, antibody on the already present bond of detection line 5 after a period of time in effluent test strips 4 is combined, antibody on control line 6 is combined.Not combined enzyme mark SA rinses to water absorption cushion 7 through buffer solution.
After the enzyme mark SA remnant in effluent test strips 4 is rinsed well, paper substrate microfluid microfluidic switch B opens, biochemical reaction microchannel B ' disconnects, reagent carrying liner H7 on microfluidic switch B touches microchannel J4 and microchannel J1, biochemical reaction channel C ' open, and the colour reagent that reagent is carried in liner H7 discharges, colour reagent is along with buffer solution is by biochemical reaction channel C ' flow to effluent test strips 4, after a period of time with effluent test strips 4 on detection line 5 and control line 6 on enzyme reaction colour developing on the enzyme mark SA that exists, show final detection result.
While not containing the antigen of being combined with specific antibody in sample, after operation, detection line 5 does not develop the color, and control line 6 colour developings do not detect antigen in sample.When detection line 5 and control line 6 all do not develop the color after operation, illustrate that this biochemical detection device lost efficacy, and cannot detect sample.
Result shows, uses the plasma EUSA double antibody sandwich method detectable antigens of hypersensitization through this device, and its sensitivity can reach every milliliter 10
-15gram (fg/ml).
The paper substrate microfluidic device of the automatic switchover passage of the present embodiment has been realized the function of paper substrate microfluid automatic switchover passage, without any need for auxiliary equipment and professional in the situation that, can introduce multiple biochemical reagents by the automatic switchover of passage, carry out multi-step biochemical reaction, reduced manual operation error, microminiaturized biochemical detection device, makes paper substrate microfluidic device possess the function of amplifying detected informational molecule.
The biochemical detection device of the present embodiment detects sample for the plasma EUSA double antibody sandwich method of hypersensitization, and it adopts modular combination to use.According to different testing goals and different detection methods, adjust or select corresponding paper substrate microfluidic device, crosslinked liner and effluent test strips, be further combined into corresponding biochemical detection device, therefore no longer enumerate corresponding embodiment at this.
Although above by general explanation and the specific embodiment, the present invention is described in detail, on basis of the present invention, can to some modifications of do and improvement, this will be apparent to those skilled in the art.Therefore, these modifications and the improvement made without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1.
a kind of paper substrate microfluidic device of the passage that automatically switches, it is characterized in that, described paper substrate microfluidic device comprises some stacked paper substrate microfluid individual layers that contain hydrophobic isolated area and hydrophilic microchannel that add, form some biochemical reaction microchannels and some resins are subject to water microchannel, microfluid bottom is provided with miniflow output; Microchannel on microfluid individual layer is provided with flap valve that three disconnections, one side are connected with hydrophobic isolated area as microfluidic switch together with the hydrophobic isolated area of microchannel correspondence position on its upper and lower two layers of paper based microfluid individual layer, in microfluidic switch, being provided with reagent carrying liner resin is subject to be provided with water-absorbing resin between water microchannel and microfluidic switch, water-absorbing resin is provided with corresponding water-absorbing resin expansion space, and microfluidic switch is provided with corresponding microfluidic switch activity space; Liquid is subject to water microchannel to flow into water-absorbing resin through resin, resin imbibition promotes microfluidic switch and reagent carrying liner downwards, reagent carrying liner move down with biochemical reaction microchannel be thereunder set contact after, open another microchannel and complete passage and automatically switch.
2.
paper substrate microfluidic device as claimed in claim 1, it is characterized in that, between described water-absorbing resin and described microfluidic switch, be provided with described water-absorbing resin expansion space, expanding in the absorb water described water-absorbing resin expansion space of backward below of described water-absorbing resin, promotes described microfluidic switch and open; Described microfluidic switch below is provided with described microfluidic switch activity space, and when described microfluidic switch starts, the described reagent carrying liner being arranged in described microfluidic switch is moved downward to described microfluidic switch activity space.
3.
paper substrate microfluidic device as claimed in claim 1, it is characterized in that, described microfluidic switch controls separately biochemical reaction microchannel or resin is subject to the break-make of water microchannel, or controls biochemical reaction microchannel simultaneously and provide the resin of water to be subject to the break-make of water microchannel for the water-absorbing resin of described microfluidic switch top.
4.
paper substrate microfluidic device as claimed in claim 1, is characterized in that, described device is subject to the width of water microchannel and length to control the described microfluidic switch opening time by controlling described resin.
5.
paper substrate microfluidic device as claimed in claim 1, it is characterized in that, when described reagent carrying liner is when arranging that thereunder biochemical reaction microchannel contacts, described reagent carrying liner is tandem with two biochemical reaction microchannels below it and is connected.
6.
paper substrate microfluidic device as claimed in claim 1, is characterized in that, described miniflow output is positioned at top layer or the bottom of described paper substrate microfluidic device.
7.
paper substrate microfluidic device as claimed in claim 1, is characterized in that, described water-absorbing resin is dome-type ball piece, rectangle, square or special-shaped water-absorbing resin.
8.
paper substrate microfluidic device as claimed in claim 1, is characterized in that, described microfluid individual layer consists of filter paper, is provided with wax layer and forms described hydrophobic isolated area on filter paper.
9.
the biochemical detection device that a kind of paper substrate microfluidic device that utilizes the arbitrary described automatic switchover passage of claim 1-8 detects, it is characterized in that, described biochemical detection device comprises matrix, and top, matrix one end is provided with paper substrate microfluidic device as buffer solution applied area; The middle part of matrix is provided with some parallel effluent test strips, and the miniflow output of paper substrate microfluidic device extends the port separating with effluent test strips equal number, and with the corresponding connection in one end of each effluent test strips; The miniflow output extending is as testing sample applied area, and it is arranged with crosslinked liner; Matrix other end top is provided with water absorption cushion, partly overlaps with effluent test strips; Wherein, the crosslinked liner under reagent in paper substrate microfluidic device carrying liner and testing sample applied area, and be arranged on detection line in effluent test strips and control line and contain as required different biochemical reagents.
10.
biochemical detection device as claimed in claim 9, is characterized in that, described water absorption cushion consists of water-absorption fiber, and the part overlapping with described effluent test strips is arranged on described effluent test strips top; Described effluent test strips material is nitrocellulose membrane, PVDF membrane or nylon membrane.
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| CN201320518179.0U CN203508047U (en) | 2013-08-23 | 2013-08-23 | Paper-based microfluid device capable of automatically switching channels and biochemical diction device made by paper-based microfluid device |
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|---|---|---|---|
| CN201320518179.0U CN203508047U (en) | 2013-08-23 | 2013-08-23 | Paper-based microfluid device capable of automatically switching channels and biochemical diction device made by paper-based microfluid device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103433085A (en) * | 2013-08-23 | 2013-12-11 | 许毅 | Paper-based microfluid device capable of automatically switching passages and application of paper-based microfluid device |
| CN105363503A (en) * | 2015-11-02 | 2016-03-02 | 华东理工大学 | Multicomponent micro droplet microfluidic chip and processing method thereof |
| CN109689214A (en) * | 2016-07-25 | 2019-04-26 | 拜昂德解决方案有限公司 | Utilize the stretchable microenvironment of multipurpose 3D of the organ-on-a-chip-device of standard si technology manufacture |
| CN112088308A (en) * | 2018-04-27 | 2020-12-15 | 株式会社优尼恩斯真 | Microchip having three-dimensional structure of paper base for detecting target antigen using immunochemical diagnostic method and target antigen detecting method using the same |
-
2013
- 2013-08-23 CN CN201320518179.0U patent/CN203508047U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103433085A (en) * | 2013-08-23 | 2013-12-11 | 许毅 | Paper-based microfluid device capable of automatically switching passages and application of paper-based microfluid device |
| CN103433085B (en) * | 2013-08-23 | 2015-04-22 | 许毅 | Paper-based microfluid device capable of automatically switching passages and application of paper-based microfluid device |
| CN105363503A (en) * | 2015-11-02 | 2016-03-02 | 华东理工大学 | Multicomponent micro droplet microfluidic chip and processing method thereof |
| CN105363503B (en) * | 2015-11-02 | 2017-10-03 | 华东理工大学 | Multicomponent microlayer model micro-fluidic chip and its processing method |
| CN109689214A (en) * | 2016-07-25 | 2019-04-26 | 拜昂德解决方案有限公司 | Utilize the stretchable microenvironment of multipurpose 3D of the organ-on-a-chip-device of standard si technology manufacture |
| CN109689214B (en) * | 2016-07-25 | 2022-12-23 | 拜昂德解决方案有限公司 | Multi-purpose 3D stretchable microenvironment for organ-on-a-chip devices fabricated using standard silicon technology |
| CN112088308A (en) * | 2018-04-27 | 2020-12-15 | 株式会社优尼恩斯真 | Microchip having three-dimensional structure of paper base for detecting target antigen using immunochemical diagnostic method and target antigen detecting method using the same |
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