CN203502270U - Letterpress type tissue chip preparation instrument - Google Patents

Abstract

The utility model relates to a letterpress type tissue chip preparation instrument. The preparation instrument is formed by connecting four parts, namely a cutting module, a miniature line cutting machine, a receptor wax mold and a feed workbench, wherein the cutting module is formed in the following way: the cutting module, a clamping screw and a hay cutter are mounted on a bottom plate, and a positioning hole and an unfilled corner are processed on the bottom plate; the receptor wax mold is formed in the following way: a plurality of empty holes are prefabricated in a whole wax block; the feed workbench comprises a transverse stepping motor, a longitudinal stepping motor, an encoder, a guide track and a ball screw; and a preparation method comprises the following steps: 1) preparing the receptor wax mold; 2) preparing a wax core; 3) placing the wax core into the receptor wax mold; 4) slicing. The letterpress type tissue chip preparation instrument provided by the utility model has the advantages of mechanical automatic operation, accurate positioning, time-saving and labor-saving properties, small errors, high efficiency, easiness in identification and small sample tissue damages.

Description

Letterpress type organization chip apparatus for preparation

Technical field

The utility model relates to a kind of important experimental technique and device in biological and medical field, specifically relates to a kind of letterpress type organization chip apparatus for preparation.

Background technology

The array of tissue also referred to as micro-array tissue (TMAS), is placed with a plurality of different tissue samples on a single acceptor piece { reception piece } or paraffin mass.With microtome, acceptor piece { reception piece } is cut into slices by usual method, obtain sample.

Micro-array tissue technology is a great experimental technique innovation, and CIin Path and biologist utilize the section of array biological tissue and Pathologic specimen to analyze, and obtain the key message of high flux biological tissue and Pathologic specimen situation and variation.

By utilizing organization chip, scientists study tissue or (with) cyto-architectural information, the problems such as the function of cell and phenotype, subcellular gene expression and protein localization and quantitative expression.

By micro-array tissue technology, a large amount of tissues and Pathologic specimen can be incorporated on a chip, thereby make laboratory possess scale histochemistry and standardization body's pathology detection method.This new technology is widely used in high-flux tissue Phenotypic examination, large-scale medicine screening, part and other biological respinse test etc.

The manufacture of tissue array is very consuming time, and this is owing to needing to use the sampling probe of hollow from various tissues or sample block in manufacturing process, and core is organized in punching press preparation, and finishing is organized after core one by one, then will organize core to be placed in same acceptor piece by both definite sequences.When sample organizes number more sometimes, this repetitive operation is wasted time and energy very much.

The manufacturing technology overwhelming majority of existing organization chip adopts sampling probe operation, and this method exists following not enough: sampling probe sampling obtains and organizes core mainly with the blind mode of wearing, and sampling spot location is inaccurate, exists representativeness of sample not high; Sampling needle samples to encircle the mode of turning and samples, although less on the impact of donor tissue piece or wax stone, is difficult to guarantee the integrality of organizing core of getting, and in the wax core of preparation, the problem such as tissue compression, distortion, fracture is serious; Sampling probe institute sampling depth is wayward in addition, although existing machinery sampler can accurately be controlled paracentesis depth, but while sampling for thinner sample, may cause wax core front end is blank wax core, therefore be prepared in organization chip and organize core not to be positioned at same plane, there is room point in the chip after section, has reduced chip preparation efficiency, is not easy to the displaying of the tissue samples such as thinner and epithelium and cyst wall.

Summary of the invention

Technical problem to be solved in the utility model is: solve the problem that above-mentioned prior art exists, and provide a kind of letterpress type organization chip apparatus for preparation, automatic and mechanical operation, accurate positioning, time saving and energy saving, error is little, and sample tissue utmost point tropism is easy to sign and identification, sample tissue damage is little, and one chip comprises multiple sample or sample tissue information.

The technical solution adopted in the utility model is:

A letterpress type organization chip apparatus for preparation, is connected to form by cutting module, micro wire cutting machine, acceptor wax-pattern, Feed table four parts; Described cutting module is that cutting board, clamping screw and chopper are installed on base plate, is also processed with pilot hole and unfilled corner on base plate; Described acceptor wax-pattern is arranged on pallet, is processed with dowel hole on pallet, and acceptor wax-pattern is on whole wax stone, to be prefabricated with a plurality of emptying apertures that are slightly larger than wax core, emptying aperture ordered arrangement; Described Feed table comprises horizontal stepper motor, longitudinal stepper motor, scrambler, guide rail and ball screw, stepper motor connects driving ball screw, ball screw connects driving guide rail again, and guide rail and pallet are connected and fixed, or the base plate of guide rail and cutting module is connected and fixed.

Principle of work:

The utility model will be placed in same acceptor wax-pattern containing a plurality of wax cores of different sample tissue, and in acceptor wax-pattern, each emptying aperture is implanted after wax core, by preheating with add wax liquid, and acceptor wax-pattern and organize core fully to merge, the wax stone of core is organized in formation more; Obtainedly contain the donor wax stone that multiple sample is organized wax core, through cooling, curing, section, film-making, obtain the high-throughout chip that contains multiple sample organizational information.

The wax core of preparing of the present utility model is to cut the unfilled corner part wire cutting donor wax stone along cutting board by line, thus the lower xsect wax core consistent with cutting board unfilled corner part size dimension of cutting, and wax core length is the thickness of donor wax stone.

In order to solve the damage of sampling probe to sample tissue, the utility model adopts the donor wax stone that the way cutting of the very little line cutting in range of influence contains sample tissue to obtain donor wax core, at finishing donor wax stone, do not contain the marginal portion of sample tissue, the cutting of employing chopper, improves the work efficiency of cutting greatly.

In order to solve a common difficult problem of preparing the direction indication difficulty of sample tissue in organization chip method, the utility model makes to start, to falling into its direction of the whole process of acceptor wax-pattern, any variation does not occur from cutting preparation containing sample tissue wax core, thereby before cutting, in donor wax stone, identified in advance the direction in inboard and the outside of sample tissue, consistent with in its maintenance direction of the wax core after this making and the sample tissue in acceptor wax block after fusion.

In order to solve wax core, to implant in this process of acceptor wax-pattern manual error large, the low difficult problem of efficiency, the utility model adopts Fully-mechanized operation, when wax core is after donor wax stone cuts down, dependence self gravitation is directly fallen into, or in the emptying aperture of the prior acceptor wax-pattern aliging with unfilled corner of operation implantation, each emptying aperture, only implant a wax core, thereby realize in acceptor wax-pattern, accurately implant the operation of sample.

When has implanted after wax core in one of them hole of acceptor wax-pattern, by the motor of operation Feed table, realize the follow-up adjacent emptying aperture of replacing and partly aim at cutting module unfilled corner, it is high that this mechanization feed motion has degree of accuracy, the advantage that efficiency is high.

advantageous effect:

The utility model does not contain the marginal portion of the donor wax stone of sample tissue in cutting, does not directly use line cutting, but first adopts chopper excision not contain the marginal portion of sample tissue, greatly increases work efficiency.

The utility model is prepared donor wax core in the way of cutting by line, and the method is because the range of influence of line cutting is less, very little to the loss of sample tissue; The wax core cutting down from donor wax stone starts to the process that falls into acceptor wax-pattern from cutting, and direction does not change, thereby wax core prepared by this device has good utmost point tropism, is convenient to identify inner side and the outside of sample tissue; Between cutting module part and acceptor wax-pattern, adopt register pin location, can quick and precisely locate; The relative motion of the relative cutting module of acceptor wax-pattern, realizes horizontal and vertical feed motion by the motor of operation Feed table, and the feed motion of this mechanization compares that manual mobile to have accuracy good, work efficiency advantages of higher.

Accompanying drawing explanation

Fig. 1 is the utility model acceptor wax-pattern mfg. moulding die structural representation;

Fig. 2 is the utility model acceptor wax-pattern structural representation;

Fig. 3 is the utility model cutting module structural representation;

Fig. 4 is the utility model acceptor wax-pattern and Feed table assembling schematic diagram;

Fig. 5 is the general assembly (GA) schematic diagram of the utility model letterpress type organization chip apparatus for preparation.

The explanation of accompanying drawing mark:

1-pallet, 2-horizontal stepper motor, 3-scrambler, 4-guide rail, 5-ball screw, 6-chopper, 7-clamping screw, 8-cutting board, 9-base plate, 10-tommy, 11-acceptor wax-pattern, 12-pilot hole, the longitudinal stepper motor of 13-, 14-counterdie, 15-unfilled corner, 16-waters wax mouth, 17-patrix, 18-cutting module pilot hole.

Embodiment

Referring to Fig. 1-Fig. 5, letterpress type organization chip apparatus for preparation of the present utility model, is connected to form by cutting module, micro wire cutting machine, acceptor wax-pattern, Feed table four parts; Described cutting module is that cutting board, clamping screw and chopper are installed on base plate, is also processed with pilot hole and unfilled corner on base plate; Described acceptor wax-pattern is arranged on pallet, is processed with dowel hole on pallet, and acceptor wax-pattern is on whole wax stone, to be prefabricated with a plurality of emptying apertures that are slightly larger than wax core, emptying aperture ordered arrangement; Described Feed table comprises horizontal stepper motor, longitudinal stepper motor, scrambler, guide rail and ball screw, stepper motor connects driving ball screw, ball screw connects driving guide rail again, and guide rail and pallet are connected and fixed, or the base plate of guide rail and cutting module is connected and fixed.

A letterpress type organization chip preparation method based on above-mentioned letterpress type organization chip apparatus for preparation, its concrete grammar is:

1) preparation of acceptor wax-pattern:

Patrix is formpiston, and counterdie is former, while manufacturing acceptor wax-pattern, first by Re Tai or constant temperature incubator, wax-pattern is preheated to 56 ℃.Upper and lower mould after preheating is closed, from the wax mouth that waters on both sides, inject fast the wax liquid melting again simultaneously, then the counterdie that slightly vibrates, makes wax liquid can fill with fully counterdie, and counterdie is filled with after wax water, upper and lower mould is at room temperature kept to static, after the abundant cooled and solidified of wax, extract patrix, thereby obtain acceptor wax-pattern, then by the order designing in advance, each emptying aperture of acceptor wax-pattern is carried out to two codings, and record;

2) preparation of wax core:

The first step is taken out donor wax stone and is positioned on cutting board from embedded box, and the both sides of donor wax stone are contacted with cutting board the right and front; Second step, screws off clamping screw, and under clamping screw, respectively puts a square plastic thin slice, is convenient to clamping screw donor wax stone is clamped; The 3rd step, because donor wax stone marginal portion is not containing sample tissue, so use thin and sharp chopper by its marginal portion excision, compares direct use line cutting and greatly increases work efficiency; The 4th step, after chopper margins of excision part, carries out the sign of sample tissue, and records to the core of containing sample tissue, facilitate follow-up identification; The 5th step; Use micro wire cutting machine along the unfilled corner wire on cutting module, thereby cut the xsect wax core consistent with unfilled corner part size dimension;

3) to acceptor wax-pattern, insert wax core:

The first step, puts into pallet by the acceptor wax-pattern preparing, and by pallet and Feed table assembly connection, or by cutting module and Feed table assembly connection; Second step, aims at the unfilled corner on cutting board by tommy with the emptying aperture that acceptor wax-pattern is positioned at first row, first row, accurately location; By parity of reasoning, after this extracts one of them pin, and mobile another pin, makes unfilled corner align one by one with another emptying aperture of first row or first row; The 3rd step, cuts wax core prepared by donor wax stone by wire cutting method, after cutting, rely on self gravitation vertically to fall, or operation is implanted in the emptying aperture of the acceptor wax-pattern aliging with unfilled corner in advance, and each specific acceptor wax block hole, only implants a wax core; The 4th step, when the emptying aperture aliging with unfilled corner on cutting board is implanted after wax core, operation stepper motor makes worktable laterally or length feed, equal in length between feeding distance and two centers, adjacent vacant hole, thus unfilled corner section aligned on the emptying aperture that realization more renews and cutting board; After finishing switching, will lock at once feeding operating grip, repeat aforesaid operations and make each emptying aperture of acceptor wax-pattern implant wax core, the wax core of implantation is all the sample tissue identifying in advance, after implanting corresponding emptying aperture, they are related to building database one to one, so that subsequent query;

4) section:

The first step, acceptor wax block and wax core tentatively merge: all emptying apertures in acceptor wax-pattern have all been implanted after wax core, and pallet is positioned in the hot platform of test or incubator, hatch fusion for 56-60 ℃, Real Time Observation, treats that wax stone bottom has wax hydrorrhea to go out, and goes to cold curing in time; Second step, wax liquid is prepared: preheating paraffin in embedding machine is melted, wax liquid temperature is adjusted to 68-72 ℃; The 3rd step, the embedding again of implanting wax stone after wax core: after preliminary fusion, can have cavity to form in wax stone, and wax stone the first half is irregular, is unfavorable for the wax stone of content of wax core to be fixed on and to complete section on microtome.Again during embedding, the preheating wax liquid in pathology embedding machine can be injected and plant in the acceptor wax-pattern of full wax core along tray wall, avoid being directly poured on wax-pattern.While injecting the wax liquid of preheating, the pallet that slightly vibrates, makes wax water fill with acceptor wax-pattern completely, avoids forming cavity; After wax liquid injects, by pallet keep static be placed under room temperature condition, be fully cooled to completely curing; The 4th step, film-making: the acceptor wax-pattern that contains wax core is taken out.After finishing mark, after merging, wax stone is fixed on section, film-making on histotome, obtains the chip of the multiple sample tissue containing in a section and the multiple sample organizational information having.

Above-mentioned micro wire cutting machine is ripe market product, and market is purchased.

Below in conjunction with accompanying drawing, the utility model is described in further detail:

As shown in the figure, the utility model is connected to form by cutting module part, micro wire cutting machine, acceptor wax-pattern, Feed table four parts; Described cutting module part is comprised of base plate, cutting board, chopper, clamping screw; On the base plate of cutting module, be equipped with in advance pilot hole, facilitate follow-up its unfilled corner part to aim at location with the emptying aperture of donor wax-pattern, reduce manual positioning time, work efficiency is provided; On cutting board, be beforehand with a unfilled corner, the size of unfilled corner is with preparing the in the same size of donor wax core; Meanwhile, this unfilled corner is partly line cutting wire route; In unfilled corner, partly contain organized donor wax stone, nexine and skin that before cutting, sign is organized in advance, after cutting on line, rely on self gravitation directly to fall in the acceptor wax-pattern of below containing the wax core of tissue, dropping process does not rotate, and wax core is square hole structure, after implantation, can not rotate displacement, thereby can not change the direction of sign, there is good utmost point tropism; Chopper Main Function in cutting module is that the edge of cutting donor wax stone does not contain the part of tissue, compares direct use line cutting work efficiency is provided greatly; The cutting of micro wire cutting machine is for cutting donor wax stone containing organized part, thereby obtains donor wax core, and to its numbering, record, uses line to cut and organize and greatly reduce the damage of cutting to tissue; Micro wire cutting machine is complete along the unfilled corner cutting of cutting die, and cut directly falls into dependence self gravitation in acceptor wax-pattern containing organized donor wax core, reduces the error of manual intervention; Feed table will make the acceptor wax-pattern can horizontal and vertical movement, and on the pallet on worktable, accomplish fluently in advance pilot hole, by register pin, cutting module and worktable are coupled together, the unfilled corner of cutting die and the hole of wax-pattern can quick and precisely be aimed at; Acceptor wax-pattern, is on monoblock wax stone, accomplishes fluently in advance the hole more bigger than wax core, make wax core can fall into smoothly in the hole of acceptor wax-pattern, each hole of wax-pattern is by the Position Number of arranging, and the wax core falling into is also numbered in advance, and correspondence establishment database is so that subsequent query; Emptying aperture in acceptor wax-pattern is piled after wax core, and wax stone is merged and embedding again, is stored up so that subsequent slice is used after cooling; During with the acceptor wax block film-making of content of wax core after merge solidifying, along the direction section perpendicular to pallet, the section obtaining like this can contain Various Tissues, thereby the section that obtains containing much information reduces time of repeatedly film-making, time saving and energy saving; Along different angles section, the chip making like this can be seen different sample tissues, the micromechanism of different angles xsect.

Claims (1)

1. a letterpress type organization chip apparatus for preparation, is characterized in that: by cutting module, micro wire cutting machine, acceptor wax-pattern, Feed table four parts, connected to form; Described cutting module is that cutting board, clamping screw and chopper are installed on base plate, is also processed with pilot hole and unfilled corner on base plate; Described acceptor wax-pattern is arranged on pallet, is processed with dowel hole on pallet, and acceptor wax-pattern is on whole wax stone, to be prefabricated with a plurality of emptying apertures that are slightly larger than wax core, emptying aperture ordered arrangement; Described Feed table comprises horizontal stepper motor, longitudinal stepper motor, scrambler, guide rail and ball screw, stepper motor connects driving ball screw, ball screw connects driving guide rail again, and guide rail and pallet are connected and fixed, or the base plate of guide rail and cutting module is connected and fixed.

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