CN203487155U - Cell separating and collecting machine - Google Patents
Cell separating and collecting machine Download PDFInfo
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- CN203487155U CN203487155U CN201320359976.9U CN201320359976U CN203487155U CN 203487155 U CN203487155 U CN 203487155U CN 201320359976 U CN201320359976 U CN 201320359976U CN 203487155 U CN203487155 U CN 203487155U
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- porous plate
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Abstract
The utility model relates to a cell separating and collecting machine. The cell separating and collecting machine comprises a storage pool, a guide tube system, a video camera, a valve system, a collector or a porous plate and a computer, wherein liquid containing cells to be separated flows through an input tube from the storage pool; liquid penetrating through the input tube is viewed by the video camera at a transparent visual part; the computer is used for acquiring video images from the video camera and analyzing the video images to detect the cells in the flowing liquid; the valve system is controlled by the computer and is opened when the cells to be separated in the liquid approach a valve, so that the liquid containing the cells to be separated enters an output tube, the cells arrive at the collector or the porous plate, and the liquid, which is not collected, enters an outlet tube; a video image system is required to control the valve system during separating. Optimally, the cell separating and collecting machine is used for separating Langerhans in diabetes study, and is also applicable to other types of cells, such as leukocytes and stem cells.
Description
Technical field
The utility model relates to a kind of cellular segregation and collector.
Background technology
Medically, especially at laboratory medicine, usually need isolated cell or cell cluster.Cell or cell cluster can be used for various objects, for example, test the drug effect of new drug.The utility model is interested is especially separated Langerhans (Langerhans) pancreas islet that contains the β cell that produces Regular Insulin from pancreas.These cells are many universities and the research topic to the interested pharmaceutical companies of exploitation diabetes medicament.The cell type of other separation interested and collection has white corpuscle, the stem cell of blood or bone marrow aspiration, tumour cell, ovum etc.
Summary of the invention
Nowadays isolated pancreatic islet is dependent on the collagenase of Isolation of pancreatic is processed, then be dependent on following methods: use microscope manually separated, the method based on gradient, Ricordi method, Quixell method, COBE cell separator or flow cytometer/magnetic cell sorting/laser.Its shortcoming is consuming time, damages the risk of tissue, and the quality of sampling pancreas islet is uneven.In addition, if the method for flow cytometer is based on cell marking, immunostaining technology for example.Therefore need automatization and sharp separation, reduce costs, reduce primary cellular defect and better paper trail.
There is system to process body fluid or the cell from people or animal.US5, has described blood draw treater in 006,107, by a strainer and tangent line flow separation machine, removes fluid and the small-particle in blood.US5,100,564 have described blood collection and treatment system, with porous-film, in conjunction with separating centrifuge system, less cell are separated from larger cell.CN101607232B has also described the separating centrifuge system with film, is mainly used to separated large and little cell, or by blood rotating centrifugal system, has had the cell of Different Weight.The utility model and existing US Patent No. 5,006,107, US5,100,564 also have the main significantly difference of Chinese patent CN101607232B to be, the large material of separated relatively small amount, can in liquid, by image method, identify, rather than need mechanical force or filter and carry out separation.Also have, the description of above three patents of invention is to be dependent on filtering technique and fluid separator.The utility model is completely different from it, because the utility model is not used strainer or tangential flow flow separator, but control those by image identification system, needs segregate research material.The utility model also has main not being both of another one, can allow object in these liquid, comprise those interested objects, for example Langerhans (Langerhans) pancreas islet flows out by a narrow pipe, interested like this object just can partly be separated, thereby by image analysis system, identified, image analysis system is controlled the final separation to interested object.Adopt the Different Light that strengthens pancreas islet and background contrasts, Langerhans (Langerhans) pancreas islet can more easily be identified.
Cellular segregation and a collector, comprising:
A storage pool, stores the liquid that contains cell to be separated;
Conduit system, comprises the input tube that contains transparent visual part, the outlet pipe of the liquid not being collected, and the output tube of collecting cell;
Video camera;
Valve system;
Collector or porous plate;
Computer;
The liquid that contains cell to be separated is from storage pool by input tube, and the liquid that passes input tube is observed by video camera in transparent visual part;
This computer obtains the video image from video camera, and video image is analyzed, to detect the cell in the liquid passing through;
Valve system is by computer control, and the cell to be separated in liquid rotates while approaching valve, allows the liquid that contains cell to be separated enter output tube, thereby cell arrives collector or porous plate; The liquid not being collected enters outlet pipe.
Cell is cell strain and the cell mass being dissolved in aaerosol solution, or unicellular.
Cell strain and cell mass are Langerhans pancreas islet, and unicellular is bone marrow stem cell, white corpuscle, and adipocyte, medullary cell, these cytolysises are in liquid.
Cell in suspension carries out chemistry in advance, and immunology or mechanicalness are processed, and to guarantee that object is easily identified, and pass through input tube together with liquid.
Carry out in advance chemistry, immunology or mechanicalness are treated to use tinting material, and histolysis agent or strainer carry out.
Video camera has high spatial resolution and temporal resolution, and its function completes by zoom lens or microscope.
By computer control pump to apply suction function in output tube.
With pre-set flow pump, liquid is sucked out from storage pool.
When the cell distributing enters in the single chamber of porous plate, an other imaging system is used to the cell in institute's extracting liq accurately to detect, to guarantee high precision.
By the movement of computer control porous plate, so that the cell of separating enters different holes.
Accompanying drawing explanation
Fig. 1. the schematic diagram of the whole system of preferred embodiment, it contains lumen, pick up camera, valve, output system and porous collector or porous plate.
The schematic diagram of the embodiment of the valve for separating of cell or cell cluster that Fig. 2-1 to Fig. 2-5 are selected.
Embodiment
Below preferred embodiment of the present utility model is described, should be appreciated that preferred embodiment described herein is only for description and interpretation the utility model, and be not used in restriction the utility model.
In Fig. 1, contain lumen, pick up camera, valve, output system and porous collector.Wherein, 1 is camera, and 2 is with the software of control pump and valve and the PC that carries out the software of data analysis, 3 is collector or porous plate, 4 for containing the storage pool that dissolves pancreatic tissue suspension, and 5 is surveyed area, and 6 is conduit, 7 is transparent tube, 8 is pump and the storage pool that contains cleaning liq, and 9 for filling the unnecessary liquid that is not collected and the chamber of object, and 10 for to be connected to pump by computer, the wire of photographic camera and porous plate shifter, 12 is piston
The utility model is included as a system of separated and collection of material, the separation of preferred cell or cell mass.A kind of preferred application example is to use a full automatic system (Fig. 1) from the separated Langerhans pancreas islet of pancreas.In its preferred embodiment, the utility model comprises the chamber of fluid of the collagenase of the pancreatic tissue that contains dissolving.In a preferred embodiment, this chamber comprises a filtering system, and it can filter out object very little in fluid or or than normal pancreas islet large object (conventionally in the scope of 75-300 micron) also.The content of this chamber is transported to a conduit system, and it is transparent in to allow the visual inside of video camera to observe the content that comes from chamber in this system, having a part at least.Pick up camera obtains the required resolving power to the interested object of observation with zoom lens or with microscope.In a preferred embodiment, with pre-set flow pump, liquid is sucked out from chamber.Also available other devices replace pump to produce by the liquid of a conduit.Conduit can be made by hard or soft material, and it can be circular, rectangle or other shapes.
Fig. 2-1 is to Fig. 2-5th, several examples of design and structure, but moreover.Cellular segregation and collector also can have other relevant design.It also has detected characteristics, according to geometric properties (size and shape), and contrast gradient, dyeing characteristic etc. is distinguished pancreas islet or other objects.6 is input pipe, and 11 is valve, and 12 is piston, make better liquid flow into outlet pipe 16,13 for to apply suction function in output tube, 14 is pancreas islet, 15 is slide block, 16 outlet pipes that are the liquid that is not collected, and 17 for collecting pancreas islet or other is collected the output tube of object.In figure, show, unidirectional arrow is shown flow direction, and four-headed arrow shows the direction of pump rotation, both can forward also can reverse rotation.
Wherein, Fig. 2 .1 shows three tunnel output systems.Valve can electronic or flexibly rotate, and fluid can the either direction in both direction flow into.Valve is rotating when pancreas islet approaches valve, allows pancreas islet enter output road and arrives collector or porous plate.Fig. 2 .2 shows that pancreas islet or other materials are separated from remaining liquid by sucking output tube 17.When pancreas islet approaches porous plate, from conduit, enter the suction startup of porous plate, make pancreas islet enter collector or porous plate by output tube 17.In Fig. 2 .3, single armed valve makes fluid enter one of two outflow pipelines, and valve rotates when pancreas islet approaches valve, makes pancreas islet enter collector or porous plate through output tube 17 wherein.Fig. 2 .4 is used the same system of fundamental sum Fig. 2 .1, has only used a two-way outflow system, is similar to threeway principle.In Fig. 2 .5, crack (slide block) system of flexible (movement), when pancreas islet is during close to crack area, slide block moves, and is equivalent to crack and moves, and makes pancreas islet enter the pipeline of collector or porous plate.Slide block also can be placed on other output channel, thereby liquid is passed through or do not pass through.
When video frequency pick-up head detects an interested target being collected for example during a pancreas islet, further lower during by a special region along pipe when pancreas islet, it can calculate accurately to it.This calculating is based on flow knowledge, by the computer of an energy control valve system and flow, is completed.Preferably this region approaches video camera very much.Valve system of this district inclusion, has drawn several embodiment in Fig. 2.The kind of valve is a lot, but preferentially the valve of two or three ports is used for sample.Object will be extracted or extract to by-pass valve control out from remaining liq, as interested pancreas islet or cell.Then cell or pancreas islet are flow to outlet system.Fig. 2 has represented several designs of valve system, and in all design and implementation examples, valve is according to the influx in pipe with visual identification goes out pancreas islet or other materials are controlled by computer.Valve can be made with any solid material, but preferably metal or transparent material.In Fig. 2 .1, drawn the design of three-way valve door.Valve can rotate with electronics or mechanical braking, thereby liquid is flowed into from one or two direction.When pancreas islet approaches valve, valve starts rotation.The rotation of valve makes pancreas islet enter collector or porous plate by pipeline. and in the design of Fig. 2 .2, pancreas islet or other materials are inhaled in second pipeline, from remaining liquid separation out.When pancreas islet approaches valve, suction function makes pancreas islet enter the pipeline of collector or porous plate.In Fig. 2 .3, a single armed valve makes fluid enter one of two outflow pipelines.Valve is opened when pancreas islet approaches valve, makes pancreas islet enter the pipeline of collector or porous plate.Fig. 2 .4 has shown the same system of fundamental sum Fig. 2 .1, has only used a two-way outflow system, is similar to threeway principle.Fig. 2 .5 has shown the crack (slide block) of flexible (movement), and when pancreas islet is during close to this region, move in crack, make pancreas islet enter the pipeline of collector or porous plate.Crack also can be placed on other output channel, thereby liquid is passed through or do not pass through.Equipment can be equipped with porous system, can provide the pancreas islet of predetermined number to porous plate or single microtubule.Motor of computer control can mobile porous system or outlet conduit and its relative position, and commander's pancreas islet enters specific hole.So just allow the pancreas islet of specific size and shape to be passed in specific hole, make the aftertreatment of pancreas islet more convenient.For example Controlling System can be configured in the following way, and 100 microns of following pancreas islet are sent to No. 1 hole on dish, and 101 to 150 microns enter hole No. 2, and 151 to 200 microns enter hole No. 3, by that analogy.So if pancreas islet is estimated as 170 microns by picture system, it will be sent to hole No. 3.Because pancreas islet is separated and classify by size, so reduced follow-up workload.Pancreas islet or cell also can be distinguished according to further feature, and for example cross-sectional geometry, dyes, or can carry out mark and identify according to mark with surface marker.Flow in outlet pipe can be determined, accurate in the position in single hole to guarantee object, or can improve system the observation of the object of outlet pipe by the second video camera.
In a preferred embodiment, this equipment is a full automatic quick and effective tripping device, for separating of Langerhans pancreas islet, or other cell or cell cluster, combine with software and allow user to arrange and monitor procedure.For Cell protection better, this device can be equipped with optional separating device under cold condition.
Generally speaking, the utility model comprises a system, in this system, wisp floats on a liquid, by the region that can carry out pattern recognition of phoresying of tube chamber, based on identification accurately, from most liquid, separate, preferably, by autocontrol valve, collecting can be in the separation vessel of single hole or porous system.
Finally it should be noted that: the foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, although the utility model is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.All within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (8)
1. cellular segregation and a collector, is characterized in that comprising:
A storage pool, stores the liquid that contains cell to be separated;
Conduit system, comprises the input tube that contains transparent visual part, the outlet pipe of the liquid not being collected, and the output tube of collecting cell;
Video camera;
Valve system;
Collector or porous plate;
Computer;
The liquid that contains cell to be separated is from storage pool by input tube, and the liquid that passes input tube is observed by video camera in transparent visual part;
This computer obtains the video image from video camera, and video image is analyzed, to detect the cell in the liquid passing through;
Valve system is by computer control, and the cell to be separated in liquid rotates while approaching valve, allows the liquid that contains cell to be separated enter output tube, thereby cell arrives collector or porous plate; The liquid not being collected enters outlet pipe.
2. cellular segregation according to claim 1 and collector, is characterized in that: cell is cell strain and the cell mass being dissolved in aaerosol solution, or unicellular.
3. cellular segregation according to claim 2 and collector, is characterized in that: cell strain and cell mass are Langerhans pancreas islet, and unicellular is bone marrow stem cell, white corpuscle, and adipocyte, medullary cell, these cytolysises are in liquid.
4. according to cellular segregation and collector described in claim 1 or 2 or 3, it is characterized in that: video camera has high spatial resolution and temporal resolution, its function completes by zoom lens or microscope.
5. according to cellular segregation and collector described in claim 1 or 2 or 3, it is characterized in that: by computer control pump to apply suction function in output tube.
6. according to cellular segregation and collector described in claim 1 or 2 or 3, it is characterized in that: with pre-set flow pump, liquid is sucked out from storage pool.
7. according to cellular segregation and collector described in claim 1 or 2 or 3, it is characterized in that: when the cell distributing enters in the single chamber of porous plate, an other imaging system is used to the cell in institute's extracting liq accurately to detect, to guarantee high precision.
8. according to cellular segregation and collector described in claim 1 or 2 or 3, it is characterized in that: by the movement of computer control porous plate, so that the cell of separating enters different holes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320359976.9U CN203487155U (en) | 2013-06-21 | 2013-06-21 | Cell separating and collecting machine |
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| CN201320359976.9U CN203487155U (en) | 2013-06-21 | 2013-06-21 | Cell separating and collecting machine |
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| CN203487155U true CN203487155U (en) | 2014-03-19 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111868504A (en) * | 2018-03-20 | 2020-10-30 | 住友电气工业株式会社 | Fine particle measuring apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111868504A (en) * | 2018-03-20 | 2020-10-30 | 住友电气工业株式会社 | Fine particle measuring apparatus |
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Granted publication date: 20140319 Termination date: 20200621 |