CN203455314U - Micro-fluidic chip analysis platform based on sonic sensor - Google Patents
Micro-fluidic chip analysis platform based on sonic sensor Download PDFInfo
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- CN203455314U CN203455314U CN201320526572.4U CN201320526572U CN203455314U CN 203455314 U CN203455314 U CN 203455314U CN 201320526572 U CN201320526572 U CN 201320526572U CN 203455314 U CN203455314 U CN 203455314U
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Abstract
The utility model discloses a micro-fluidic chip analysis platform based on a sonic sensor. The platform comprises five parts, namely a sonic sensor, a sample injection chip and a temperature control unit thereof, a periphery sample injection system, a sensor signal collecting and processing system and a platform supporting assembly, wherein the multichannel sonic sensor and the polymer sample injection chip are bonded through a solvent to satisfy a high-throughput test of micron-scale or millimeter-scale liquid samples. The reaction condition of a fluid is controlled by the integrated or independent temperature control unit, and the periphery sample injection system is used for automatically realizes functions of driving samples and a buffer liquid in a chip, quantitively transferring liquid of the sample, cleaning a sampling needle and the like. The platform disclosed by the utility model prevents signal distortion caused by interference of optical factors in conventional detection methods by combining technologies such as sonic sensing, magnetic bead immunization and micro-fluidic chip, and meanwhile satisfies the requirements of quickly and accurately measuring complex samples by combining immune magnetic beads.
Description
Technical field
The utility model belongs to microfluidic analysis technical field, and what be specifically related to is a kind of microfluidic chip analysis platform based on sonic sensor.
Background technology
At present life science and diagnostic application field, especially quantification of protein aspect, proposed new requirement to detecting sensor and system thereof, comprises higher measurement sensitivity, response speed and the many aspects such as microminiaturized faster.Traditional optical test method is widely used as ELISA, Western Blot and Luminex, but above-mentioned method of testing is difficult to avoid occurring because of the interference of optical considerations the possibility of distorted signals, cause obtaining pg magnitude hypersensitivity in complex sample test process and become difficult, above-mentioned method of testing is very consuming time simultaneously, needs multistep manual operations could obtain test result.
Micro-fluidic chip technology is in addition integrated by basic operation units such as sample preparation related in the fields such as biological, chemistry and medical analysis, reaction, separation, detections, reach the object of high flux, express-analysis sample, started to play an increasingly important role in fields such as bioanalysis, medical science detection, food and drug safety and environmental pollutions.Mostly the research of the micro-fluidic chip based on sonic sensor is both at home and abroad to concentrate on detecting sensor sensitivity raising, sample preparation and hybrid reaction at present, sample is controlled one or several directions such as driving, chip manufacture material and means, and relevant technology is integrated and propose seldom to relate to by commercial analysis platform.
Utility model content
The purpose of this utility model is to overcome the above problem that prior art exists, and a kind of microfluidic chip analysis platform based on sonic sensor is provided, by relevant Technology Integration, and commercialization.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the utility model is achieved through the following technical solutions:
A microfluidic chip analysis platform based on sonic sensor, comprises peripheral sampling system, sonic sensor, collecting sensor signal and disposal system, platform supporting component and sample introduction chip.
Described sample introduction chip comprises base plate, from left to right sets gradually sample chamber, micro-threshold switch, liquid storage tank on described base plate, and the edge of described base plate arranges the gentle liquid pipeline that rushes of waste liquid pipeline; Described sample introduction chip adopts polymeric material, microchannel width and height dimension scope are 10 μ m-5mm, described sample chamber, described liquid storage tank and microchannel are integrated in cover plate 5-1, and described cover plate 5-1 and described substrate 5-2 adopt the mode bonding of bonding, thermocompression bonding, ultrasonic bond or laser bonding; Described sample introduction chip adopts integral type or free-standing temperature conditioning unit, and wherein the fluid temperature (F.T.) of sensor, sample chamber and microchannel is controlled in integrative temp .-controlled unit simultaneously, and temperature range is 25-37 ℃; In stand alone type temperature conditioning unit, sensor and sample chamber arrange respectively attemperating unit, temperature range 25-37 ℃ wherein, and for the thermostatic control of reacting, sample chamber temperature range 65-90 ℃, reacts for PCR.
Further, described sonic sensor is a kind of in flexible board wave device, surface acoustic wave device, thin film bulk acoustic resonator part, the microchannel of described sonic sensor adopts electromagnet or permanent magnet, described permanent magnet comprises permanent magnet, permanent magnet bracket and permanent magnet bracket guide rail, described flexible board wave device and described sample introduction chip adopt solvent bonding, and described flexible board wave device comprises ground electrode, interdigital electrode and external electrode.
Further, described sample introduction chip and described peripheral sampling system can be selected single channel or hyperchannel, and hyperchannel is one of binary channels, four-way, six passages, eight passages, ten passages and 16 passages.
Further, realize by probe support accurate location when many group probe multi-electrode signal parallels gather, except probe tip is with the contact conducting of sensor respective electrode place, other positions of probe and needle guard apply insulating material, insulating material is selected Parylene Parylene, and model is one of tri-kinds of Parylene C, Parylene N and Parylene D.
Further, sample introduction chip 1-5 support to adopt the disturbance of vibration proof platform signal when avoiding sample analysis, and analysis platform is done the anti-stop signal of shielding processing and disturbed simultaneously, and wherein screening type is one of electric shield, magnetic shielding and electromagnetic screen.
The beneficial effects of the utility model are:
The utility model combines sound wave sensing, the technology such as magnetic bead immunity and micro-fluidic chip, wherein sonic sensor utilizes the variation of vibration of thin membrane frequency can obtain the content of the testing sample of adsorption, avoided traditional detection method as ELISA, the distorted signals that Western Blot etc. bring because of optical considerations interference, while binding immunoassay magnetic bead meets complex sample and measures fast and accurately requirement, the utility model adopts modular design, be applicable to the analysis of multiple yardstick single channel or Multi-channel liquid sample, will be in bioanalysis, medical science detects, the field such as food and drug safety and environmental pollution plays an increasingly important role.
Accompanying drawing explanation
Fig. 1 is analysis platform one-piece construction schematic diagram;
Fig. 2 is analysis platform working state schematic representation;
Fig. 3 is a kind of ten passage sample introduction chip schematic diagram based on acoustic wave device;
Fig. 4 is a kind of front elevation of ten passage flexible board wave devices;
Fig. 5 is a kind of rear view of ten passage flexible board wave devices;
Fig. 6 is a kind of above-mentioned flexible board wave device and sample introduction chip connection diagram;
Fig. 7 is a kind of sample introduction chip integrative temp .-controlled cellular construction schematic diagram;
Fig. 8 is a kind of front elevation of sample introduction chip stand alone type temperature conditioning unit structure;
Fig. 9 is a kind of rear view of sample introduction chip stand alone type temperature conditioning unit structure;
Figure 10 is sensor multi-electrode signal parallel acquisition probe device schematic diagram;
Figure 11 is that magnetic field, sensor microchannel adopts electromagnet structure schematic diagram;
Figure 12 is that magnetic field, sensor microchannel adopts permanent magnet structural representation.
Number in the figure explanation: 1-1, peripheral sampling system, 1-2, sonic sensor, 1-3, collecting sensor signal and disposal system, 1-4, platform supporting component, 1-5, sample introduction chip, 3-1, sample chamber, 3-2, liquid storage tank, 3-3, micro-threshold switch, 3-4, waste liquid pipeline, 3-5, damping fluid pipeline, 4-1, ground electrode, 4-2, interdigital electrode, 4-3, external electrode, 4-4, sensor fluid channel, 5-1, cover plate, 5-2, substrate, 6-1, integrative temp .-controlled unit, 7-1, sensor temperature conditioning unit, 7-2, sample chamber temperature conditioning unit, V1/V2, T-valve, V3/V4, micro-threshold switch, V5/V6, micro-threshold switch, 10-1, probe support, 10-2, probe needle guard, 10-3, probe tip, 10-4, register pin, 10-5, probe support supported hole, 11-1, electromagnet core, 12-1, permanent magnet, 12-2, permanent magnet bracket, 12-3, permanent magnet bracket guide rail.
Embodiment
Below with reference to the accompanying drawings and in conjunction with the embodiments, describe the utility model in detail.
Shown in Fig. 1, a kind of microfluidic chip analysis platform based on sonic sensor, comprises peripheral sampling system 1-1, sonic sensor 1-2, collecting sensor signal and disposal system 1-3, platform supporting component 1-4 and sample introduction chip 1-5.
Peripheral sampling system 1-1 is used syringe pump to drive, adopt T-valve or be integrated in the break-make that micro-valve on sample introduction chip 1-5 is controlled fluid, wherein separate type sampling system adopts a syringe pump to realize the cleaning that quantitatively moves liquid and sample sampling probe of sample, a syringe pump is realized sample and the driving of damping fluid in sample introduction chip, and integral type sampling system is only used a syringe pump sequentially to realize above-mentioned functions.
MEMS sonic sensor is the core devices of system, acoustic wave device type for liquid testing comprises that flexible board wave device, surface acoustic wave device and thin film bulk acoustic resonator part are several, wherein Fig. 4, Fig. 5 are a kind of ten passage flexible board wave device typical structures, 4-1 is ground electrode, for the grounding of multi-group electrode, conventionally select Mo metal.External electrode 4-3 introduces electric signal through too much organizing interdigital electrode 4-2, and by inverse piezoelectric effect, device film produces vibration.Testing sample or damping fluid are through the sensor fluid channel 4-4 of sonic sensor 1-2, the variation of membrane resonant frequency makes opposite side interdigital electrode obtain corresponding electric signal by piezoelectric effect, and drawing by external electrode 4-3 respective electrode, thereby reaction or determine the variation of adhering to quality of film surface.While adopting many group probes to carry out the collection of multi-electrode signal parallel to sensor, because MEMS sensor physical dimension is limited, need to consider the accurate location of each probe.As shown in figure 10, by probe support 10-1, realize, the position of opening of probe support itself has determined the lateral attitude that probe is final, except probe tip 10-3 is with the contact conducting of sensor respective electrode place, other positions of probe and probe needle guard 10-2 apply insulating material, insulating material is selected Parylene Parylene, and model is one of tri-kinds of Parylene C, Parylene N and Parylene D, and coating thickness scope is 0.5-10 μ m.Four probe support supported hole 10-5 coordinate after straight pins the coarse positioning as probe support, the home position contacting between the straight pin capable of regulating probe tip of different length and sensor electrode, shown a pair of register pin 10-4 is for fine positioning, realizes the accurate cooperation of probe support and micro-fluidic chip the corresponding interface.
A magnetic field is all contained in each microchannel of MEMS sonic sensor, is used for catching the magnetic bead of adsorption target testing sample.Then close magnetic field, under the mobilization of sample, the magnetic bead that is loaded with target testing sample is combined and continues to stay sensor surface with sensor surface generation biologic specificity, and other magnetic bead can be by fluid removal.Wherein to apply form be one of permanent magnet or electromagnet in magnetic field, and it is upper that electromagnet core 11-1 winding around is also fixed on probe support 10-1, and as shown in figure 11, wherein electric signal break-make controlling magnetic field puts on and removes; Described permanent magnet comprises permanent magnet 12-1, permanent magnet bracket 12-2 and permanent magnet bracket guide rail 12-3, and testing sample magnetic bead possesses enough absorption affinities when guaranteeing magnetic field to reaction, conventionally selects permanent magnet mode to apply magnetic field.As shown in figure 12, be different from electromagnet, permanent magnet 12-1 is by approaching or coming controlling magnetic field to apply and remove away from MEMS sensor surface.
Collecting sensor signal and disposal system 1-3 test and analyze for the amplitude versus frequency characte of multichannel microchannel sample test.
Platform supporting component 1-4 adopts the disturbance of vibration proof platform signal when avoiding sample analysis, and analysis platform is done the anti-stop signal of shielding processing and disturbed simultaneously, and wherein screening type is one of electric shield, magnetic shielding and electromagnetic screen.
Fig. 3 is a kind of ten passage sample introduction chip schematic diagram based on acoustic wave device, sample introduction chip 1-5 adopts polymeric material, microchannel width and height dimension are not limited to micron dimension, range of size is 10 μ m-5mm, sample chamber 3-1, liquid storage tank 3-2 and microchannel are integrated in cover plate, and cover plate and substrate adopt the mode bonding of bonding, thermocompression bonding, ultrasonic bond or laser bonding.Testing sample splashes into from sample chamber 3-1, and each sample chamber design volume is 500 μ L.Damping fluid can be used in the sensor surface trim that dissociates, and through external container, from buffering liquid pipeline 3-5, introduces, and finally by micro-threshold switch 3-3 and testing sample, switches.Liquid storage tank 3-2 stores sample or damping fluid by the driving of outside sampling system, design Storage volume 1.2mL.After having reacted, liquid storage tank liquid is discharged and is reclaimed through waste liquid pipeline 3-4.Through the switching of micro-valve, sample introduction chip has been realized the auto-flushing of damping fluid, thereby reuses.Sample introduction chip 1-5 adopts integral type or free-standing temperature conditioning unit, and as shown in Figure 7, integrative temp .-controlled unit 6-1 controls the fluid temperature (F.T.) of sensor, sample chamber and microchannel simultaneously, and temperature range is 25-37 ℃.As shown in Figure 9, in stand alone type temperature conditioning unit, sensor and sample chamber arrange respectively attemperating unit, and the temperature range 25-37 ℃ of sensor temperature conditioning unit 7-1 place wherein, for the thermostatic control of reacting, the temperature range 65-90 ℃ of temperature conditioning unit 7-2 place, sample chamber, reacts for PCR.
Further, sample introduction chip and peripheral sampling system can be selected single channel or hyperchannel, and hyperchannel is one of binary channels, four-way, six passages, eight passages, ten passages and 16 passages.
As shown in Figure 2, analysis platform duty, sample introduction chip enters definite position along direction shown in horizontal arrow, collecting sensor signal and disposal system 1-3 place moving structure move down, guarantee accurately to coordinate with each interface of sample introduction chip, comprise that signal acquisition probe parts, magnetic field apply parts, and liquid storage tank and micro-threshold switch are all realized the corresponding one by one of position.
Fig. 6 is a kind of flexible board wave device and sample introduction chip connection diagram, the two adopts solvent bonding mode, wherein sonic sensor 1-2 and cover plate 5-1 are bonding by cementing agent, sample and damping fluid transmit along the direction of arrow in the figure, enter the sensor fluid channel 4-4 of sonic sensor 1-2 through cover plate 5-1 microchannel.
Claims (6)
1. the microfluidic chip analysis platform based on sonic sensor, it is characterized in that: comprise peripheral sampling system (1-1), sonic sensor (1-2), collecting sensor signal and disposal system (1-3), platform supporting component (1-4) and sample introduction chip (1-5), described sample introduction chip (1-5) comprises base plate, on described base plate, from left to right set gradually sample chamber (3-1), micro-threshold switch (3-3), liquid storage tank (3-2), the edge of described base plate arranges waste liquid pipeline (3-4) and buffering liquid pipeline (3-5).
2. the microfluidic chip analysis platform based on sonic sensor according to claim 1, it is characterized in that: described sample introduction chip (1-5) adopts polymeric material, microchannel width and height dimension scope are 10 μ m-5mm, described sample chamber (3-1), described liquid storage tank (3-2) and microchannel are integrated in cover plate (5-1), and described cover plate (5-1) and substrate (5-2) adopt the mode bonding of bonding, thermocompression bonding, ultrasonic bond or laser bonding; Described sample introduction chip (1-5) adopts integral type or free-standing temperature conditioning unit, and wherein the fluid temperature (F.T.) of sensor, sample chamber (3-1) and microchannel is controlled in integrative temp .-controlled unit simultaneously, and temperature range is 25-37 ℃; In stand alone type temperature conditioning unit, sensor and sample chamber (3-1) arrange respectively attemperating unit, temperature range 25-37 ℃ wherein, and for the thermostatic control of reacting, sample chamber temperature range 65-90 ℃, reacts for PCR.
3. the microfluidic chip analysis platform based on sonic sensor according to claim 1, it is characterized in that: described sonic sensor (1-2) is flexible board wave device, surface acoustic wave device, a kind of in thin film bulk acoustic resonator part, the microchannel of described sonic sensor (1-2) adopts electromagnet or permanent magnet, described permanent magnet comprises permanent magnet (12-1), permanent magnet bracket (12-2) and permanent magnet bracket guide rail (12-3), described flexible board wave device and described sample introduction chip (1-5) adopt solvent bonding, described flexible board wave device comprises ground electrode (4-1), interdigital electrode (4-2) and external electrode (4-3).
4. microfluidic chip analysis platform according to claim 1, it is characterized in that: described sample introduction chip (1-5) can be selected single channel or hyperchannel with described peripheral sampling system (1-1), and hyperchannel is one of binary channels, four-way, six passages, eight passages, ten passages and 16 passages.
5. microfluidic chip analysis platform according to claim 1, it is characterized in that: realize by probe support more the accurate location while organizing the collection of probe multi-electrode signal parallel, except probe tip is with the contact conducting of sensor respective electrode place, other positions of probe and needle guard apply insulating material, insulating material is selected Parylene Parylene, and model is one of tri-kinds of Parylene C, Parylene N and Parylene D.
6. microfluidic chip analysis platform according to claim 1, it is characterized in that: sample introduction chip (1-5) support to adopt the disturbance of vibration proof platform signal when avoiding sample analysis, analysis platform is done the anti-stop signal interference of shielding processing simultaneously, and wherein screening type is one of electric shield, magnetic shielding and electromagnetic screen.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103454346A (en) * | 2013-08-27 | 2013-12-18 | 中国科学院苏州生物医学工程技术研究所 | Micro-fluidic chip analysis platform based on sonic sensor |
| CN104498353A (en) * | 2014-11-05 | 2015-04-08 | 中国科学院苏州生物医学工程技术研究所 | Isothermal-amplification piezoelectric nucleic acid detection system |
| CN104593254A (en) * | 2015-01-20 | 2015-05-06 | 天津农学院 | Integrated multi-parameter test platform for microbes |
| CN107979352A (en) * | 2016-10-24 | 2018-05-01 | 天津大学 | A kind of micro-fluidic mixing arrangement of film bulk acoustic |
| CN108023565A (en) * | 2016-10-31 | 2018-05-11 | 三星电机株式会社 | Wave filter including bulk acoustic wave resonator |
| CN109557330A (en) * | 2018-12-14 | 2019-04-02 | 贵州大学 | A kind of three axis mobile platforms for high-throughput droplet array microfluid point sample |
| CN111208119A (en) * | 2020-02-25 | 2020-05-29 | 北京京东方传感技术有限公司 | Digital microfluidic chemiluminescence detection chip, detection method and detection device |
| CN112840386A (en) * | 2018-10-18 | 2021-05-25 | 日东电工株式会社 | Data acquisition method and signal measurement system |
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2013
- 2013-08-27 CN CN201320526572.4U patent/CN203455314U/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103454346A (en) * | 2013-08-27 | 2013-12-18 | 中国科学院苏州生物医学工程技术研究所 | Micro-fluidic chip analysis platform based on sonic sensor |
| CN103454346B (en) * | 2013-08-27 | 2015-10-28 | 中国科学院苏州生物医学工程技术研究所 | A kind of microfluidic chip analysis platform based on sonic sensor |
| CN104498353A (en) * | 2014-11-05 | 2015-04-08 | 中国科学院苏州生物医学工程技术研究所 | Isothermal-amplification piezoelectric nucleic acid detection system |
| CN104593254A (en) * | 2015-01-20 | 2015-05-06 | 天津农学院 | Integrated multi-parameter test platform for microbes |
| CN104593254B (en) * | 2015-01-20 | 2016-06-22 | 天津农学院 | Microorganism multi-parameter comprehensive test platform |
| CN107979352A (en) * | 2016-10-24 | 2018-05-01 | 天津大学 | A kind of micro-fluidic mixing arrangement of film bulk acoustic |
| CN108023565A (en) * | 2016-10-31 | 2018-05-11 | 三星电机株式会社 | Wave filter including bulk acoustic wave resonator |
| CN108023565B (en) * | 2016-10-31 | 2021-07-13 | 三星电机株式会社 | Filter comprising a bulk acoustic wave resonator |
| CN112840386A (en) * | 2018-10-18 | 2021-05-25 | 日东电工株式会社 | Data acquisition method and signal measurement system |
| CN112840386B (en) * | 2018-10-18 | 2023-10-24 | 日东电工株式会社 | Data acquisition method and signal measurement system |
| CN109557330A (en) * | 2018-12-14 | 2019-04-02 | 贵州大学 | A kind of three axis mobile platforms for high-throughput droplet array microfluid point sample |
| CN111208119A (en) * | 2020-02-25 | 2020-05-29 | 北京京东方传感技术有限公司 | Digital microfluidic chemiluminescence detection chip, detection method and detection device |
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