CN203382763U - CIK (Cytokine-Induced Killer) cell activation kit - Google Patents
CIK (Cytokine-Induced Killer) cell activation kit Download PDFInfo
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- CN203382763U CN203382763U CN201320400012.4U CN201320400012U CN203382763U CN 203382763 U CN203382763 U CN 203382763U CN 201320400012 U CN201320400012 U CN 201320400012U CN 203382763 U CN203382763 U CN 203382763U
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- cell activation
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Abstract
The utility model relates to a CIK (Cytokine-Induced Killer) cell activation kit comprising a kit body, a kit cover and a reagent rack, wherein the kit body is hinged with the kit cover; the reagent rack is fixedly arranged in the kit body and provided with five insertion holes in which reagent bottle fixing sleeves are arranged; each reagent bottle fixing sleeve comprises a cylinder and an annular sponge cushion, wherein the annular sponge cushion is fixedly connected to the inner wall of the cylinder, the upper edge of the cylinder is provided with an annular bulge, and an annular buckle is arranged below the annular bulge; the reagent bottle fixing sleeves and the reagent rack are buckled through the annular buckle. The CIK cell activation kit provided by the utility model is filled with lymphocyte separating medium, serum-free medium, recombinant human IFN (Interferon)-gamma, recombinant human IL (Interleukin)-2 and CD3 monoclonal antibodies, so that the CIK cell cultivation time can be effectively shortened; reagent bottles in the kit are peripherally and axially fixed through the reagent bottle fixing sleeves, so that the safety of the kit bottles in a transportation process is greatly improved.
Description
Technical field
The utility model relates to the test kit technical field, especially a kind of CIK cell activation test kit.
Background technology
In prior art, the normal employing used lymphocyte separation medium, serum free medium, recombinant human il-2, CD3 monoclonal antibody, recombinanthumanifn-γ and recombinant human IL-1 α to be induced activation to the CIK cell, and lymphocyte separation medium is the peripheral blood mononuclear cell from collecting in patient body for separating of purifying; Serum free medium is cultivated the required nutrient of CIK cell for providing; The recombinant human il-2 plays an important role to the cytotoxic activity that maintains the CIK cell, and can stimulate growth and the propagation of CIK cell; The CD3 monoclonal antibody can analogue antigen and identification and the activation process of TCR/CD3 mixture, thereby causes propagation and the activation of T cell, is stimulus indispensable in the CIK cell cultures; The recombinanthumanifn-γ has the rise peripheral blood lymphocyte and shows the effect that IL-2R expresses, and can strengthen susceptibility and the intensity of T cell to the short proliferative response of IL-2, in the CIK cell cultivation process, adds the recombinanthumanifn-γ can reduce the consumption of IL-2.
In research process, find, the effect of recombinant human IL-1 α is identical with the recombinant human il-2, for growth and the propagation that stimulates the CIK cell, is not to add, and does not affect growth and the propagation of CIK cell after removing, can be cost-saving.
Along with going deep into the CIK cell research, the inducing and cultivating also along with stdn gradually of CIK cell, be necessary to be positioned in the same reagent box according to the standard proportioning cultivating required lymphocyte separation medium, serum free medium, recombinanthumanifn-γ, recombinant human il-2 and the CD3 monoclonal antibody of CIK cell, without configuring separately again mentioned reagent, greatly reduce the risk that in the responsible degree of operation and culturing process, cell is polluted when carrying out the CIK cell cultures.
Simultaneously, the test kit used in prior art can not be well fixing by the axial and circumferential of reagent bottle, in the long-distance transport process, due to its buffering poor seismic behavior, easily causes being scattered of reagent, thereby pollute whole test kit, causes a large amount of wastes.
The utility model content
The technical problems to be solved in the utility model is: in order to overcome the problems of the prior art, provide a kind of simple in structure, easy to use and CIK cell activation test kit that Transport Safety is high.
The utility model solves the technical scheme that its technical problem adopts: a kind of CIK cell activation test kit, comprise box body, lid and reagent rack, described box body and lid are hinged, described reagent rack is fixedly installed in box body, offer five jacks on described reagent rack, be provided with the reagent bottle permanent sleeve in described jack, described reagent bottle permanent sleeve comprises cylindrical shell and annular sponge pad, described annular sponge pad is fixedly connected on cylinder inboard wall, described cylindrical shell upper limb is provided with annular protrusion, described annular protrusion below is provided with annular buckle, described permanent sleeve and reagent rack are by annular buckle clamping.
Particularly, described reagent rack is the plastics reagent rack, can give the toughness that reagent rack is stronger; When running into violent vibrations or rocking, can better reduce the reagent shaking degree in test kit simultaneously.
Particularly, described jack upside is provided with label, on described label, indicates respectively: lymphocyte separation medium, serum free medium, recombinanthumanifn-γ, recombinant human il-2 and CD3 monoclonal antibody.Lymphocyte separation medium and serum free medium are arranged in two macropores of reagent rack, and the consumption of recombinanthumanifn-γ, recombinant human il-2 and CD3 monoclonal antibody is little with respect to lymphocyte separation medium and serum free medium consumption, therefore be arranged in three little jacks.
Particularly, described reagent rack is cavity body, in described reagent rack cavity body, is provided with the buffering cavernous body, can further strengthen circumferential the fixing of reagent bottle permanent sleeve.
Particularly, described box body, lid and reagent rack end face are square or circle.
The beneficial effects of the utility model are: a kind of CIK cell activation test kit of the present utility model, be mounted with lymphocyte separation medium, serum free medium, recombinanthumanifn-γ, recombinant human il-2 and CD3 monoclonal antibody in test kit, can effectively shorten the time of cultivating the CIK cell, the circumferential and axial that test kit completes reagent bottle by the reagent bottle permanent sleeve are fixed, and have greatly improved reagent bottle security in transportation.
The accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is further illustrated.
Fig. 1 is the stereographic map of a kind of CIK cell activation test kit of the present utility model;
Fig. 2 is the stereographic map of a kind of CIK cell activation test kit reagent rack of the present utility model;
Fig. 3 is the sectional view of a kind of CIK cell activation test kit reagent bottle permanent sleeve of the present utility model.
In figure: 1. box body, 2. lid, 3. reagent rack, 31. jacks, 32. labels, 4. reagent bottle permanent sleeve, 41. cylindrical shells, 42. annular sponge pads, 43. annular protrusions, 44. annular buckles.
Embodiment
By reference to the accompanying drawings the utility model is described in further detail now.These accompanying drawings are the schematic diagram of simplification, basic structure of the present utility model only is described in a schematic way, so it only show the formation relevant with the utility model.
Embodiment 1
As shown in Figure 1 to Figure 3, a kind of CIK cell activation test kit, comprise box body 1, lid 2 and reagent rack 3, box body 1, lid 2 and reagent rack 3 end faces are square, box body 1 and lid 2 are hinged, and reagent rack 3 is fixedly installed in box body 1, and reagent rack 3 is the plastics reagent rack, reagent rack 3 is cavity body, in reagent rack 3 cavity body, is provided with the buffering cavernous body; Offer five jacks 31 on reagent rack 3, jack 31 upsides are provided with label 32, on label, indicate respectively: lymphocyte separation medium, serum free medium, recombinanthumanifn-γ, recombinant human il-2 and CD3 monoclonal antibody; Be provided with reagent bottle permanent sleeve 4 in jack 31, reagent bottle permanent sleeve 4 comprises cylindrical shell 41 and annular sponge pad 42, annular sponge pad 42 is fixedly connected on cylindrical shell 41 inwalls, cylindrical shell 41 upper limbs are provided with annular protrusion 43, annular protrusion 43 belows are provided with annular buckle 44, and reagent bottle permanent sleeve 4 and reagent rack 3 are by annular buckle 44 clampings.
The above-mentioned foundation desirable embodiment of the present utility model of take is enlightenment, and by above-mentioned description, the relevant staff can, in the scope that does not depart from this utility model technological thought, carry out various change and modification fully.The technical scope of this utility model is not limited to the content on specification sheets, must determine its technical scope according to the claim scope.
Claims (5)
1. a CIK cell activation test kit, comprise box body (1), lid (2) and reagent rack (3), described box body (1) and lid (2) are hinged, described reagent rack (3) is fixedly installed in box body (1), it is characterized in that: offer five jacks (31) on described reagent rack (3), be provided with reagent bottle permanent sleeve (4) in described jack (31), described reagent bottle permanent sleeve (4) comprises cylindrical shell (41) and annular sponge pad (42), described annular sponge pad (42) is fixedly connected on cylindrical shell (41) inwall, described cylindrical shell (41) upper limb is provided with annular protrusion (43), described annular protrusion (43) below is provided with annular buckle (44), described reagent bottle permanent sleeve (4) and reagent rack (3) are by annular buckle (44) clamping.
2. CIK cell activation test kit as claimed in claim 1, it is characterized in that: described reagent rack (3) is the plastics reagent rack.
3. CIK cell activation test kit as claimed in claim 1, it is characterized in that: described jack (31) upside is provided with label (32).
4. CIK cell activation test kit as claimed in claim 1, it is characterized in that: described reagent rack (3) is cavity body, in described reagent rack (3) cavity body, is provided with the buffering cavernous body.
5. as the described CIK cell activation of claim 1-4 any one test kit, it is characterized in that: described box body (1), lid (2) and reagent rack (3) end face are square or circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320400012.4U CN203382763U (en) | 2013-07-05 | 2013-07-05 | CIK (Cytokine-Induced Killer) cell activation kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320400012.4U CN203382763U (en) | 2013-07-05 | 2013-07-05 | CIK (Cytokine-Induced Killer) cell activation kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203382763U true CN203382763U (en) | 2014-01-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320400012.4U Expired - Lifetime CN203382763U (en) | 2013-07-05 | 2013-07-05 | CIK (Cytokine-Induced Killer) cell activation kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203382763U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104698162A (en) * | 2015-03-30 | 2015-06-10 | 哈尔滨工业大学（威海） | Combined kit |
| WO2016088062A1 (en) * | 2014-12-02 | 2016-06-09 | 陈剑 | An electric shock pipe and cell electroporator having electric shock pipe |
| WO2016088061A1 (en) * | 2014-12-02 | 2016-06-09 | 陈剑 | Cell electroporator |
-
2013
- 2013-07-05 CN CN201320400012.4U patent/CN203382763U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016088062A1 (en) * | 2014-12-02 | 2016-06-09 | 陈剑 | An electric shock pipe and cell electroporator having electric shock pipe |
| WO2016088061A1 (en) * | 2014-12-02 | 2016-06-09 | 陈剑 | Cell electroporator |
| US10472601B2 (en) | 2014-12-02 | 2019-11-12 | Jian Chen | Shock tube and cell electroporation device with the shock tube |
| US11965149B2 (en) | 2014-12-02 | 2024-04-23 | Jian Chen | Shock tube and cell electroporation device with the shock tube |
| CN104698162A (en) * | 2015-03-30 | 2015-06-10 | 哈尔滨工业大学（威海） | Combined kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140108 |
|
| CX01 | Expiry of patent term |