CN203337597U - Sensing chip used for detecting mercury ions and based on L-cysteine - Google Patents
Sensing chip used for detecting mercury ions and based on L-cysteine Download PDFInfo
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- CN203337597U CN203337597U CN2013204164482U CN201320416448U CN203337597U CN 203337597 U CN203337597 U CN 203337597U CN 2013204164482 U CN2013204164482 U CN 2013204164482U CN 201320416448 U CN201320416448 U CN 201320416448U CN 203337597 U CN203337597 U CN 203337597U
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- sensing chip
- rete
- cys
- film layer
- cysteine
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Abstract
The utility model discloses a sensing chip used for detecting mercury ions and based on L-cysteine. The sensing chip comprises a gold film substrate (1) modified by an L-cysteine film layer (16), one corner of a gold layer of the gold film substrate (1) is connected with a metal wire (2) wrapped by a plastic insulating layer (3), and the connected position is wrapped and sealed by epoxy resin adhesive (4); a SiO2 film layer (12), a metal chromium film layer (13), a copper-nickel alloy film layer (14) and a gold film layer (15) are precipitated on a polished silicon nitride substrate (11) in a sputtering mode step by step to form the gold film substrate (1), the modifying L-cysteine film layer (16) is assembled on the surface of the gold film layer (15) after the gold film layer (15) is chemically cleaned and processed, and therefore the sensing chip used for detecting mercury ions and based on L-cysteine is formed. The sensing chip used for detecting mercury ions and based on L-cysteine is easy to manufacture, low in cost, convenient to use and capable of being used for fast detecting the micro amount of mercury ions in the environment.
Description
Technical field
The utility model relates to a kind of sensing chip that detects mercury ion based on Cys, belongs to the chemical/biological field of sensing technologies, is applicable to Environmental security and detects.
Background technology
Mercury pollution is a global problem, and due to its severe toxicity and bioconcentration, it has received increasing concern.Dimercurion (Hg
2+) be the most stable inorganic mercury, be present in natural water body Hg
2+even micro-concentrations also can produce toxicity, it can be accumulated in vivo, by food chain transport in human body, and in human body, the Trace Mercury of accumulation can't be drained by self metabolism, to directly cause heart, liver, thyroid disease, cause nerve problems, chronic mercury poisoning, even cause the formation of malignant tumour.Secondly, the ecological methylate methyl mercury of the microorganism of mercury ion, be also a kind of strong toxin, can be delivered in the tissue of fish and marine mammal by food chain, is converted into hypertoxic organic mercury.Therefore, can quick and precisely analyze the content of beary metal in the medium such as water body in living environment, be of great significance for the life quality, the environment tool of preserving our planet that improve the mankind.
The method that detects metal mercury ions has a lot, as atomic spectroscopy, inductively coupled plasma atomic emission spectrum (ICP-AES method), spectrophotometric method, high performance liquid chromatography, inductively coupled plasma mass spectrometry (ICPMS), chemoluminescence method and fluorometry etc.Yet these methods often need expensive exact instrument, complicated sample preparation flow process and skilled operating personnel, can not or inconvenience carry out out of doors fast detecting.For this reason, the utility model is modified golden film substrate surface by the unimolecule self-assembling method by the Cys assembling, forms a kind of sensing chip that detects mercury ion based on Cys.This sensing chip making is simple, with low cost, easy to use, sensitivity is very high, be better than traditional instrument analytical method, the fast detecting that can be used for Trace Mercury ion in environment has very important application prospect in the fields such as environmental monitoring, agricultural food product and biomedicine.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of sensing chip based on Cys detection mercury ion simple, that cost is low of making, and can be used for Environmental security and detects.
In order to achieve the above object, the technical solution adopted in the utility model is: a kind of sensing chip that detects mercury ion based on Cys, it is characterized in that: this sensing chip comprises the golden film substrate that a Cys rete is modified, golden layer one jiao of this gold film substrate is connected with the tinsel wire that is surrounded by plastic insulating layer outward, junction epoxide-resin glue environmental sealing; Described golden film substrate is sputtering sedimentation SiO progressively on the silicon nitride board of polishing
2rete, crome metal rete, cupronickel rete, golden membranous layer, golden membranous layer is assembled in its surface and is modified the Cys rete again after chemical cleaning is processed, thereby forms the sensing chip that detects mercury ion based on Cys.Wherein, described golden film substrate be shaped as square, its length of side is 6 ~ 12mm, the material of tinsel wire is copper wire, aluminium wire or filamentary silver.And, the SiO of sputtering sedimentation progressively on the silicon nitride board of polishing
2the thickness of rete is 80 ~ 380nm, and the thickness of crome metal rete is 20 ~ 60nm, and the thickness of cupronickel rete is 80 ~ 380nm.Especially, the cupronickel rete is after polishing, and the thickness of institute's deposited gold rete is 120 ~ 400nm in its surface; Golden membranous layer is after chemical cleaning is processed, and assembling in its surface the amount of modifying the Cys rete is 5 ~ 60ug.
Aspect the Design & preparation of sensing chip, different from other method is, the utility model proposes a kind of assembling method of modifying of simple Cys rete, first the golden membranous layer surface of sensing chip gold film substrate is cleaned with redistilled water, successively at redistilled water, in absolute ethyl alcohol, ultrasonic cleaning is each 1 ~ 10 minute, then take out in the Cys/ethanolic solution that immerses 1.0 ~ 100mmol/L, in 4 ℃ of refrigerators, self assembly is 2 ~ 48 hours, take out dry, obtain the sensing chip that the Cys rete is modified, the used time is not placed in deionized water in 4 ° of C refrigerator sealing preservations.
The beneficial effects of the utility model are, this Nanometer scale Au film electrode is made simple, with low cost, easy to use, and golden film flatness is high, easily carry out finishing, sensitivity is very high, be better than traditional instrument analytical method, the fast detecting that can be used for Trace Mercury ion in environment has very important application prospect in the fields such as environmental monitoring, agricultural food product and biomedicine.
The accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is further illustrated.
Fig. 1 is based on the planar structure schematic diagram that Cys detects the sensing chip of mercury ion.
Fig. 2 is based on the cross-sectional view of golden film substrate that Cys detects the sensing chip of mercury ion.
In Fig. 1, the golden film substrate that 1. the Cys rete is modified, 2. tinsel wire, 3. plastic insulating layer, 4. epoxide-resin glue.
In Fig. 2,11. silicon nitride boards, 12. SiO
2rete, 13. crome metal retes, 14. cupronickel retes, 15. golden membranous layers, 16. Cys retes, 17. Cys molecules.
Embodiment
embodiment 1
The assembling preparation of sensing chip:
As shown in Figure 1, detect the sensing chip of mercury ion based on Cys, this sensing chip comprises the golden film substrate 1 that a Cys rete 16 is modified, golden layer one jiao of this gold film substrate 1 is connected as copper wire wire 2 with the tinsel that is surrounded by plastic insulating layer 3 outward, epoxide-resin glue 4 environmental sealings for junction.Epoxide-resin glue 4 used is connected one jiao, the gold layer of golden film substrate 1 with copper wire wire 2 sealed envelope, to guarantee good conduction, conduction.
As shown in Figure 2, for golden film substrate 1, it is shaped as square, and the selection length of side is 6mm, is the SiO of sputtering sedimentation 300nm progressively on the silicon nitride board 11 of polishing
2the crome metal rete 13 of rete 12,50nm, the cupronickel rete 14 of 180nm; Cupronickel rete 14 golden membranous layer 15 of sputtering sedimentation 150nm in its surface again after polishing; Golden membranous layer 15 is after chemical cleaning is processed, and Cys rete 16 is modified in assembling in its surface, and its quality is 27ug.
It should be noted that the assembling method of modifying of Cys rete 16, first golden membranous layer 15 surfaces of sensing chip gold film substrate 1 are cleaned with redistilled water, successively in redistilled water, absolute ethyl alcohol ultrasonic cleaning each 3 minutes, then take out in the Cys/ethanolic solution that immerses 20mmol/L, in 4 ℃ of refrigerators, self assembly is 24 hours, take out drying, obtain the sensing chip that Cys rete 16 is modified, the used time is not placed in deionized water in 4 ° of C refrigerators sealing preservations.
embodiment 2
The detection of mercury ion:
Adopt conventional electrochemical primary cells test macro: the sensing chip that the Cys rete 16 that the said method assembling is modified is modified is working electrode, saturated calomel electrode is as contrast electrode, carry out the electrochemical potential test, the potential response value of this sensing chip electrode and the concentration of mercury ion are 1.0 * 10
-9~ 1.0 * 10
-5linear in the mol/L scope, the detection lower limit of mercury ion is reached to 3.4 * 10
-10mol/L.
embodiment 3
The reappearance of sensing chip:
The sensing chip that 10 Cys retes 16 that assembling is modified are as stated above modified is working electrode, and saturated calomel electrode is as contrast electrode, respectively to 2.0 * 10
-6mol/L and 1.0 * 10
-5the mercury ion solution example of mol/L carries out the electrochemical potential test, can find out, after carrying out continuously measuring for 10 times, relative standard deviation is respectively 0.54% and 0.51%, shows the favorable reproducibility of this sensing chip detection method.
embodiment 4
The selectivity of sensing chip:
The sensing chip that the Cys rete 16 that assembling is modified is as stated above modified is working electrode, and saturated calomel electrode, as contrast electrode, has been investigated respectively the impact that Common Cations is measured mercury ion, finds K
+, Ca
2+, Na
+, Mg
2+, Ba
2+, Cu
2+, Fe
2+, Ag
+, Zn
2+, Bi
3+, Co
2+, Sr
2+, Mn
2+, NH
4 +interference Deng 16 metal ion species is not obvious, shows that the selectivity of this sensing chip detection method is good.
embodiment 5
The mensuration of mercury ion in the environmental wastewater sample:
The sensing chip that the Cys rete 16 that assembling is modified is as stated above modified is working electrode, saturated calomel electrode is as contrast electrode, the mark-on of having investigated respectively mercury ion in eight water samples detects, and the recovery, between 97.9 ~ 104.8%, illustrates that the method recovery is good; And contrast with the standard dithizone spectrophotometry, the measurement result of sample is more consistent, meets the requirement of quantitative test, illustrates that this sensing chip can be applicable to the detection of mercury ion in real wastewater samples.
Claims (7)
1. a sensing chip that detects mercury ion based on Cys, it is characterized in that: this sensing chip comprises the golden film substrate (1) that a Cys rete (16) is modified, golden layer one jiao of this gold film substrate (1) is connected with the tinsel wire (2) that is surrounded by plastic insulating layer (3) outward, epoxide-resin glue for junction (4) environmental sealing.
2. sensing chip according to claim 1 is characterized in that: described golden film substrate (1) is sputtering sedimentation SiO progressively on the silicon nitride board (11) in polishing
2rete (12), crome metal rete (13), cupronickel rete (14), golden membranous layer (15), golden membranous layer (15) is assembled in its surface and is modified Cys rete (16) again after chemical cleaning is processed.
3. sensing chip according to claim 1 is characterized in that: described golden film substrate (1) be shaped as square, its length of side is 6 ~ 12mm.
4. sensing chip according to claim 1, it is characterized in that: the material of described tinsel wire (2) is copper wire, aluminium wire or filamentary silver.
5. sensing chip according to claim 2 is characterized in that: the SiO of sputtering sedimentation progressively on the silicon nitride board (11) of polishing
2the thickness of rete (12) is 80 ~ 380nm, and the thickness of crome metal rete (13) is 20-60nm, and the thickness of cupronickel rete (14) is 80 ~ 380nm.
6. sensing chip according to claim 2, it is characterized in that: cupronickel rete (14) is after polishing, and the thickness of institute's deposited gold rete (15) is 120 ~ 400nm in its surface.
7. sensing chip according to claim 2 is characterized in that: golden membranous layer (15) is after chemical cleaning is processed, and the amount of assembling in its surface modification Cys rete (16) is 5 ~ 60ug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013204164482U CN203337597U (en) | 2013-07-15 | 2013-07-15 | Sensing chip used for detecting mercury ions and based on L-cysteine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013204164482U CN203337597U (en) | 2013-07-15 | 2013-07-15 | Sensing chip used for detecting mercury ions and based on L-cysteine |
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| Publication Number | Publication Date |
|---|---|
| CN203337597U true CN203337597U (en) | 2013-12-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103336039A (en) * | 2013-07-15 | 2013-10-02 | 长沙理工大学 | Sensing chip method for detecting mercury ions based on L-cysteine |
| CN105675676A (en) * | 2016-01-22 | 2016-06-15 | 湖南大学 | Mercuric ion or cysteine detecting electrochemical DNA biosensor and manufacturing method and application thereof |
-
2013
- 2013-07-15 CN CN2013204164482U patent/CN203337597U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103336039A (en) * | 2013-07-15 | 2013-10-02 | 长沙理工大学 | Sensing chip method for detecting mercury ions based on L-cysteine |
| CN103336039B (en) * | 2013-07-15 | 2016-01-27 | 长沙理工大学 | A kind of sensing chip method detecting mercury ion based on Cys |
| CN105675676A (en) * | 2016-01-22 | 2016-06-15 | 湖南大学 | Mercuric ion or cysteine detecting electrochemical DNA biosensor and manufacturing method and application thereof |
| CN105675676B (en) * | 2016-01-22 | 2018-04-13 | 湖南大学 | Electrochemical DNA biosensor for detecting mercury ion or cysteine and its preparation method and application |
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|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131211 Termination date: 20140715 |
|
| EXPY | Termination of patent right or utility model |