CN203337512U - Single-light-source multi-channel optical system applied to flow type analyzer - Google Patents
Single-light-source multi-channel optical system applied to flow type analyzer Download PDFInfo
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- CN203337512U CN203337512U CN2013203723978U CN201320372397U CN203337512U CN 203337512 U CN203337512 U CN 203337512U CN 2013203723978 U CN2013203723978 U CN 2013203723978U CN 201320372397 U CN201320372397 U CN 201320372397U CN 203337512 U CN203337512 U CN 203337512U
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Abstract
The utility model discloses a single-light-source multi-channel optical system applied to a flow type analyzer. The single-light-source multi-channel optical system comprises a multi-wavelength LED (Light-Emitting Diode) light source, wherein the multi-wavelength light emitted by the multi-wavelength LED light source passes through a light equalizing module, and penetrates through a coupling lens to enter optical waveguide; the optical waveguide is used for transmitting the light to a cylindrical lens; the cylindrical lens is used for transmitting the light to a flow chamber. According to the single-light-source multi-channel optical system applied to the flow type analyzer disclosed by the utility model, the light emitting function formed by the combination of multiple laser devices is realized by using a single LED light source; the rectangular shape of a light source luminous surface is ensured by an unique standard LED single-chip technology; rectangular light spots with uniform illumination are formed in the flow chamber, so that the problems that the detection missing of particles is formed by oval focusing light spots in flow cytometry adopting the laser device and the longitudinal dimensions of the light spots are extremely large are radically solved; detection signal fluctuation caused by Gaussian distribution of the light intensity of conventional laser light spots is effectively reduced; the detection precision is improved.
Description
Technical field
The utility model relates to a kind of optical system, and what be specifically related to is a kind of single light source Multi-channel optical system that is applied to flow type analyzer.
Background technology
The LASER Light Source adopted in flow cytometry at present, its emission light beam, by dichroscope, optical filter, catoptron, focus lamp etc., is directed into many optical detection zones, realizes the irradiation of particle to be measured.Spectral characteristic due to the uniqueness of the many fluorescent dyes that use in flow cytometer, and for the specific parameter analysis of particle to be measured, usually must adopt more than one excitation wavelength, classic method is to adopt a plurality of different wave length laser instruments, be equipped with the optical systems such as independent dichroscope, optical filter, catoptron for each laser instrument, and be equipped with separately independently free-space optical systems for each laser, perhaps optical waveguide, the perhaps combination of free space and optical waveguide, in order to be sent to the sample measurement zone by light.The utilization of a plurality of LASER Light Source, on the one hand greatly system cost, LASER Light Source and supporting optical system have increased system bulk greatly on the other hand.
The utility model content
The purpose of this utility model is to overcome the above problem that prior art exists, a kind of single light source Multi-channel optical system that is applied to flow type analyzer is provided, adopt the multi-wavelength LED light source, and by optics mixed light focus module, can obtain the function of a plurality of laser instrument combinations with a LED light source, realize the multi-wavelength excitation of single light source, overcome the shortcoming that the laser emitting wavelength is limited, volume is large, cost is high.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the utility model is achieved through the following technical solutions:
A kind of single light source Multi-channel optical system that is applied to flow type analyzer, comprise the multi-wavelength LED light source, the light that described multi-wavelength LED light source sends the multi-wavelength enters optical waveguide through even optical module by coupled lens, described optical waveguide transmits light to lens pillar, and described lens pillar transmits light to flow chamber.
Further, described even optical module, coupled lens, optical waveguide, lens pillar is on the same level axle.
Further, the light that described multi-wavelength LED light source sends is rectangular light.
The beneficial effects of the utility model are:
The utility model has been realized the emission function of a plurality of laser instrument combinations with single LED light source, unique accurate single-chip technology of LED, guaranteed the rectangular shape of light source luminescent face, can pass through optical system, form the rectangular light spot of uniform-illumination in flow chamber, fundamentally changed the undetected and hot spot longitudinal size excessive problem of particle that in the flow cytometry that adopts laser instrument, oval-shaped focal beam spot forms, effectively reduce the fluctuation that conventional laser hot spot light intensity is the detection signal that Gaussian distribution causes, improved accuracy of detection.
The accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the light distribution schematic diagram that conventional laser adopts the oval hot spot of cylindrical lens focus formation;
Fig. 3 is the light distribution schematic diagram of the rectangle focal beam spot of employing rectangle light-emitting area LED light source of the present invention;
The multi-wavelength emission LED light source schematic diagram that Fig. 4 is the single light source that adopts of the present invention.
Number in the figure explanation: 1, multi-wavelength LED light source, 2, even optical module, 3, coupled lens, 4, optical waveguide, 5, lens pillar, 6, flow chamber.
Embodiment
Below with reference to the accompanying drawings and in conjunction with the embodiments, describe the utility model in detail.
Shown in Fig. 1, a kind of single light source Multi-channel optical system that is applied to flow type analyzer, comprise multi-wavelength LED light source 1, the light that described multi-wavelength LED light source 1 sends the multi-wavelength enters optical waveguide 4 through even optical module 2 by coupled lens 3, described optical waveguide 4 transmits light to lens pillar 5, and described lens pillar 5 transmits light to flow chamber 6.
Further, described even optical module 2, coupled lens 3, optical waveguide 4, lens pillar 5 is on the same level axle.
Shown in Fig. 4, the light that described multi-wavelength LED light source sends is rectangular light, the surface of light source type is arranged as rectangle, and wherein λ 1, and λ 2 ... λ 7 represents respectively the luminescence chip of different centre wavelengths, each luminescence chip is shaped as also rectangle, and it,, by common condenser lens, just can form the uniform rectangular light spot of light distribution, effectively avoid conventional laser hot spot light intensity to be the fluctuation of the detection signal that Gaussian distribution causes, improved accuracy of detection.All chip common anode utmost points of LED light source connect, the other end is provided with separately independently control circuit and controls separately (n1, n2 ... n7), can be by connect one or several LED chip sometime, realize multi-wavelength emission, and realize the adjusting of light source center wavelength is controlled by control circuit.The group number of LED chip can be 2 groups, 3 groups, 4 groups, or other any amount, is illustrated as the schematic diagram of 7 groups, by integrated not several LED chip on the same group, can realize the different progression of adjusting, and obtains multiple emission wavelength.
In the multi-wavelength emission LED light source of single light source, the LED chip of different emission wavelengths, can connect and cut off by high speed, realizes the high speed switching of different wave length light beam.Because response time of LED chip is nanosecond order, therefore by the high speed commutation circuit, can realize the even light switching of the different wave length of higher frequency of 100k-1MHz.This embodiment, being much better than current flow cytometer adopts a plurality of LASER Light Source to carry out the structure of different wave length light beam switching, for adopting different excitation wavelengths to carry out the system of high speed multiparameter detection to the same fluorescence probe, there is exercisable practical significance.
Embodiment 1
As shown in Figure 2, wherein the particle in flow chamber is perpendicular to transverse, propagate along the ellipse short shaft direction, light intensity becomes in the elliptical shaped laser spot of Gaussian distribution, when same particle along the minor axis landing, during perhaps along the edge landing, different light signals, the accuracy of severe jamming measurement result will be produced.In general flow type analyzer, the ellipse light spot major diameter is about 60-100 μ m, and minor axis diameter 10-20 μ m, compress circular laser facula by cylindrical lens and obtain, and is guaranteeing require major axis long as far as possible, and minor axis to be short as far as possible under power demand density prerequisite.The laser power generally adopted at present generally only has tens milliwatts, and the long power density that will affect focal beam spot of major axis, and minor axis is excessive likely has over a particle in the same time and enters the hot spot focal zone, interferometry.
As shown in Figure 3, focal beam spot is the uniform rectangular light spot of light distribution.LED light source power of the present invention reaches several watts, is far longer than the power of laser instrument milliwatt magnitude, has solved the inadequate problem of focal beam spot place power density.And its rectangle light-emitting area has the inherent advantage than circular laser facula, more easily focus on to form the uniform rectangular light spot of light distribution, fundamentally solved in the flow cytometry of current employing LASER Light Source oval Gauss's hot spot to detecting impact.
Claims (3)
1. a single light source Multi-channel optical system that is applied to flow type analyzer, it is characterized in that: comprise multi-wavelength LED light source (1), the light that described multi-wavelength LED light source (1) sends the multi-wavelength enters optical waveguide (4) through even optical module (2) by coupled lens (3), described optical waveguide (4) transmits light to lens pillar (5), and described lens pillar (5) transmits light to flow chamber (6).
2. the single light source Multi-channel optical system that is applied to flow type analyzer according to claim 1, it is characterized in that: described even optical module (2), coupled lens (3), optical waveguide (4), lens pillar (5) is on the same level axle.
3. the single light source Multi-channel optical system that is applied to flow type analyzer according to claim 1, it is characterized in that: the light that described multi-wavelength LED light source (1) sends is rectangular light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203723978U CN203337512U (en) | 2013-06-27 | 2013-06-27 | Single-light-source multi-channel optical system applied to flow type analyzer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013203723978U CN203337512U (en) | 2013-06-27 | 2013-06-27 | Single-light-source multi-channel optical system applied to flow type analyzer |
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| CN203337512U true CN203337512U (en) | 2013-12-11 |
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| CN2013203723978U Expired - Lifetime CN203337512U (en) | 2013-06-27 | 2013-06-27 | Single-light-source multi-channel optical system applied to flow type analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323395A (en) * | 2013-06-27 | 2013-09-25 | 中国科学院苏州生物医学工程技术研究所 | Single-light-source multi-channel optical system applied to flow analyzer |
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2013
- 2013-06-27 CN CN2013203723978U patent/CN203337512U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323395A (en) * | 2013-06-27 | 2013-09-25 | 中国科学院苏州生物医学工程技术研究所 | Single-light-source multi-channel optical system applied to flow analyzer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131211 |
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| CU01 | Correction of utility model | ||
| CU01 | Correction of utility model |
Correction item: Termination upon expiration of patent Correct: Revocation of Patent Expiration and Termination False: On July 14, 2023, the expiration and termination of 39 volumes and 2802 issues of the patent Number: 28-02 Volume: 39 |