CN203316140U - Microfluidic chip grafted with polyelectrolyte brush on surface of micro-channel to inhibit electroosmotic flow - Google Patents
Microfluidic chip grafted with polyelectrolyte brush on surface of micro-channel to inhibit electroosmotic flow Download PDFInfo
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- CN203316140U CN203316140U CN2013202725317U CN201320272531U CN203316140U CN 203316140 U CN203316140 U CN 203316140U CN 2013202725317 U CN2013202725317 U CN 2013202725317U CN 201320272531 U CN201320272531 U CN 201320272531U CN 203316140 U CN203316140 U CN 203316140U
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- polyelectrolyte brush
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Abstract
The utility model relates to a microfluidic chip grafted with a polyelectrolyte brush on the surface of a micro-channel to inhibit an electroosmotic flow, and belongs to the microfluidic field. The microfluidic chip comprises a sample inlet pool, an inlet runner, a slit runner, an outlet runner and a liquid waste pool sequentially communicated. A polyelectrolyte brush with positive ions is grafted on the runner surface in the slit runner, and a uniform electric field is applied to the direction parallel to the runner to drive the fluid to flow. Under the effect of the uniform electric field, the polyelectrolyte brush grafted on the surface of the micro-channel extends along the direction of the electric field, and the fluid in the micro-channel is acted by viscous friction and electrostatic attraction of the polyelectrolyte brush, so that surface charges in the runner are balanced, reduced or inhibited. With attraction between solvent particles and the polyelectrolyte brush, the solvent particles permeate to the polyelectrolyte brush layer, so that the purpose of inhibiting the electroosmotic flow is realized. According to the microfluidic chip provided by the utility model, the separation accuracy of proteins and DNA (Deoxyribonucleic Acid) molecules can be improved, and the microfluidic chip has the advantages of wide applicability, strong controllability and the like.
Description
Technical field
The utility model relates to micro-fluidic field, the particularly micro-fluidic chip of EOF in a kind of effective inhibition fluid channel, espespecially a kind of micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush.Thereby but balance, minimizing or suppress the EOF of generation in fluid channel material surface institute electronegative inhibition fluid channel can be applicable to the fields such as protein analysis separation, DNA separation.
Background technology
EOF is because the fluid channel material surface is electronegative, and when electric field is added on fluid, electrostatic charge is driven by Coulomb force and the fluid that causes flows.In micro-fluidic chip, suppress EOF and can improve efficiency, reappearance and the separating degree of separation.And, in applications such as electroosmotic pumps, increase EOF and can improve pump performance, contribute to electrophoretic separation, chromatography.
In recent years, the micro-fluidic chip development rapidly, is used widely in fields such as medical treatment, national defence, pharmacy, biochemical analysis.Study the fluid channel surface that shows the polyelectrolyte brush modification and can realize the EOF regulation and control.Research is found, under the effect of vertical electric field and transverse electric field, different grafting densities, different polymerization degree and different electric-field intensity, can cause the variation of polyelectrolyte brush conformation, can reach the purpose of regulation and control EOF (referring to Qianqian Cao, ChunchengZuo, Lujuan Li, Yinhe Zhang, Modulation of electroosmotic flow by electric field-responsive polyelectrolyte brushes:a molecular dynamic study, MicrofluidNanofluid, 2012, 12, 649-655, Qianqian Cao, ChunchengZuo, LUjuan Li, Yinhe Zhang, Guang Yan, Electro-osmotic Flow in Nanochannels with Voltage-Controlled Polyelectrolyte Brushes:Dependence on Grafting Density and Normal Electric Field, Polymer Physics, 2012,50,805-811, Zhao Zhang, ChunchengZuo, Qianqian Cao, Yanhong Ma, Shuqing Chen, Modulation of Electroosmotic Flow Using Polyelectrolyte Brushes:A Molecular Dynamics Study, Macromolecular, 2012,21,145-152).In addition; neutral polymer brush under the different solvents condition also can be to fluid channel in EOF influential (referring to Qianqian Cao; ChunchengZuo, Lujuan Li, Yanhong Ma; Nanli; Electroosmotic flow in a nanofluidic channel coated with neutral polymers, MicrofluidNanofluid, 2010; 9,1051-1062; Qianqian Cao, ChunchengZuo, Lujuan Li, Yang Yang, Nan Li, Controlling electroosmotic flow by polymer coating:a dissipative particle dynamics study, MicrofluidNanofluid, 2011,10,977-990).Above research shows that fluid channel surface grafting polymerization thing brush can increase EOF, yet there are no the report that suppresses EOF.
The research that capillary electrophoresis separation commonly used suppresses electric osmose mainly contains: the people such as Milos V.Novotny by contain on the capillary wall of silicon grafting neutral polymer coating suppress EOF (referring to Novotny et al. Suppression of Electroosmosis with Hydrolytically Stable Coatings:United States, 5074982. [P]. 1991-12-24).2010, in " Suppression of Electro-Osmotic Flow by Surface Roughness " that the people such as R.J.Messinger deliver in " Physical Review Letters ", the method of the roughness by changing the fluid channel surface, reached the effect that suppresses EOF.Dolnlk etc. adopt similar method, the boric acid of different polymerization degree is adsorbed onto to water passage surface, can effectively suppress the Zeta electromotive force, thereby reach the purpose that suppresses EOF (referring to Dolnik et al. Separation Medium for Capillary Electrophoresis Suppressing Electroosmotic Flow in Bare Capillaries and Channels:United States, 2013/0001084 A1. [P]. 2013-1-3).Said method can suppress EOF really to a certain extent, but material surface modifying in fluid channel and electric pulse field parameter are controlled to comparatively complexity, can't automatically regulate EOF and suppress degree when extra electric field changes.So, need at present a kind of micro-fluidic chip of effective inhibition fluid channel electroendosmosis stream that can the self adaptation extra electric field badly.If can effective inhibition EOF, will be to Separation of Proteins, analysis, the applications such as DNA separation play important impetus.
Summary of the invention
The purpose of this utility model is to provide a kind of micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush, solved the EOF method of inhibitioning operating difficulties, accuracy that prior art exists low, the aspects such as electric-field intensity are required to the problem such as harshness.The utility model is a kind of micro-fluidic chip of effective inhibition EOF of self adaptation extra electric field, by fluid channel inner surface grafting polyelectrolyte brush, suppressing EOF, has that precision is high, controllability strong and advantages of simple structure and simple.
Above-mentioned purpose of the present utility model is achieved through the following technical solutions:
The micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush, comprise that the sample body enters unit, slit conduit unit, sample body deliverying unit and drives electric field, described sample body enters unit and comprises sample inlet pool 1 and entrance channel 2, and described entrance channel 2 two ends are connected with slit conduit 3 with sample inlet pool 1 respectively; Described slit conduit unit comprises slit conduit 3 and polyelectrolyte brush 4, described slit conduit 3 two ends are connected with outlet flow 5 with entrance channel 2 respectively, the water passage surface of described slit conduit 3 is with functional groups, can grafting polyelectrolyte brush 4, described polyelectrolyte brush 4 is with cation, and can be grafted on by physisorphtion or chemical bonding the water passage surface of slit conduit 3; Described sample body deliverying unit comprises outlet flow 5 and waste liquid pool 6, and described outlet flow 5 is connected with waste liquid pool 6 with slit conduit 3 respectively; Described driving electric field is the uniform electric field 7 applied being parallel to slit conduit 3 directions.
The length of described entrance channel 2
, width
.
The length of described slit conduit 3
, width
.
The length of described outlet flow 5
, the width of outlet flow 5
.
Described slit conduit 3 inner surface material are Graphene, silicon wafer, quartz, aluminium, gold, glass, natural rubber, carbon black, epoxy resin or dimethyl silicone polymer.
Described for the polyelectrolyte brush 4 of modifying slit conduit 3 surfaces for cationic polyelectrolyte brush, be specially poly 2 vinyl pyridine, butyl rubber, polyvinyl alcohol, polyvinyl acetate, crystal methamphetamine, acrylic resin, the polymethoxy ether, PEO, poly-sulfuration cyclopropane, poly-4-methoxyl group storax is rare, poly-2-methyl-1-propylene, poly-1, 3 dioxolanes, polyisobutene, Poly alpha Olefins (PAO), poly-alkyl vinyl ether, poly-trityl carbonium ion, polyacrylamide, PMAm, polyvinyl acetate, poly N-vinyl, poly-methyl oxygen base ethylacrylic acid or poly-hexadecyldimethyl benzyl ammonium ammonium chloride.
Described physisorphtion refers to by polyelectrolyte brush terminal functional groups and substrate surface absorption.
Described chemical bonding comprises and is grafted to surface (graft to) and from surface grafting (graft from), being grafted to the fluid channel surface that surface refers to the responding property after will be the synthetic polyelectrolyte brush with functional group is with modification reacts, from surface grafting, refer to prepare activity functional groups on the fluid channel surface, form the initator monolayer by condensation reaction, and, under the effect of initator, monomer forms polymer on the fluid channel surface.
Described polyelectrolyte brush 4 grafts on the grafting density on slit conduit 3 surfaces
.
The intensity of described uniform electric field 7
.
In the utility model, fluid enters micro-fluidic chip from sample inlet pool 1, through entrance channel 2, arrives slit conduit 3.In slit conduit 3, by near the reciprocation of the charged ion additional uniform electric field power and slit conduit 3 inner surfaces, on the fluid channel surface, form electric double layer, produce driving force, drive the fluid in slit conduit 3 to flow.Due to polyelectrolyte brush 4, with cation, slit conduit 3 surface charges are balanced, reduce or suppress.Under uniform electric field 7 effects, the polyelectrolyte brush 4 on slit conduit 3 surfaces has stretching, extension to a certain degree along direction of an electric field, and along with electric-field intensity increase gradually, polyelectrolyte brush 4 extension degrees increase, and plays the obstruction fluid and passes through, and suppresses the effect of EOF.Therefore, by controlling electric-field intensity, can effectively control the inclination angle that polyelectrolyte brush 4 height and polyelectrolyte brush 4 and water passage surface form, thus the flow of fluid in quantitative control runner.Then, fluid enters outlet flow 5, finally flows into waste liquid pool 6.
The beneficial effects of the utility model are: can greatly improve the control accuracy of fluid channel inner fluid flow, the situation of the different electric-field intensity of self adaptation, different grafting density, different fluid channel size condition Fluid field; Reduce the complexity that current EOF suppresses structure, the difficulty of operation, and there is the advantages such as simple in structure, that precision is high and controllability is strong.
The accompanying drawing explanation
Accompanying drawing described herein is used to provide further understanding of the present utility model, forms the application's a part, and illustrative example of the present utility model and explanation thereof, for explaining the utility model, do not form improper restriction of the present utility model.
Fig. 1 is basic structure schematic diagram of the present utility model.
Fig. 2 is the concrete structure schematic diagram of the utility model slit conduit.
Fig. 3 is each component particle design sketch in the utility model slit conduit.
Fig. 4 is in the utility model slit conduit in different polyelectrolyte brush grafting density situations, runner fluid velocity inside curve map.
Fig. 5 is in the utility model slit conduit in different polyelectrolyte brush grafting density situations, polyelectrolyte brush monomer densogram in runner.
In figure: 1, sample inlet pool; 2, entrance channel; 3, slit conduit; 4, polyelectrolyte brush; 5, outlet flow; 6, waste liquid pool; 7, uniform electric field; 8, fluid particles.
The specific embodiment
Further illustrate detailed content of the present utility model and the specific embodiment thereof below in conjunction with accompanying drawing.
Shown in Figure 1, the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush of the present utility model, comprise the sample inlet pool 1, entrance channel 2, slit conduit 3, outlet flow 5, the waste liquid pool 6 that are communicated with successively, in slit conduit 3, water passage surface is by the method for physical absorption or chemical bonding, grafting is with cationic polyelectrolyte brush 4, applies uniform electric field 7 and flows with drive fluid being parallel to the runner direction.
The length of described entrance channel 2
, width
.
The length of described slit conduit 3
, width
.
The length of described outlet flow 5
, width
.
Described slit conduit 3 surfaces, can the grafting polyelectrolyte brush with the surface-functional group.The fluid channel inner surface material is Graphene, silicon wafer, quartz, aluminium, gold, glass, natural rubber, carbon black, epoxy resin or dimethyl silicone polymer.
Described for the polyelectrolyte brush 4 of modifying slit conduit 3 surfaces for cationic polyelectrolyte brush, be specially poly 2 vinyl pyridine, butyl rubber, polyvinyl alcohol, polyvinyl acetate, crystal methamphetamine, acrylic resin, the polymethoxy ether, PEO, poly-sulfuration cyclopropane, poly-4-methoxyl group storax is rare, poly-2-methyl-1-propylene, poly-1, 3 dioxolanes, polyisobutene, Poly alpha Olefins (PAO), poly-alkyl vinyl ether, poly-trityl carbonium ion, polyacrylamide, PMAm, polyvinyl acetate, poly N-vinyl, poly-methyl oxygen base ethylacrylic acid or poly-hexadecyldimethyl benzyl ammonium ammonium chloride.
Described polyelectrolyte brush 4, be grafted to slit conduit 3 surfaces by it, and the grafting method adopted comprises physisorphtion and chemical bonding.Wherein physisorphtion is adsorbed by physical action by polyelectrolyte brush terminal functional groups and fluid channel surface; Chemical bonding comprises and is grafted to surface (graft to) and from surface grafting (graft from), the former is that the fluid channel surface of will be the synthetic polyelectrolyte brush with functional group the responding property after with modification reacts, the latter prepares activity functional groups on the fluid channel surface, form the initator monolayer by condensation reaction, and, under the effect of initator, monomer forms polymer on the fluid channel surface.
Shown in Figure 2, described polyelectrolyte brush 4 grafts on slit conduit 3, should control its surface grafting density
, wherein
For grafting degree, i.e. the number of polyelectrolyte brush on unit are,
For LJ potential energy length parameter in theoretical research.Now, due to steric effect, between polyelectrolyte brush, meeting, can be by end to stretching with the runner vertical direction for fear of overlapping.
Institute's direction of an electric field that applies, for being parallel to slit conduit 3 directions, and is uniform electric field, and intensity is
, both guaranteed effectively drive fluid of extra electric field, avoid again extra electric field that the chemical bond connected between the polyelectrolyte brush monomer is broken.
Embodiment 1:
Shown in Figure 1, the utility model comprises sample inlet pool 1, entrance channel 2, slit conduit 3, polyelectrolyte brush 4, outlet flow 5, waste liquid pool 6, is parallel to the uniform electric field 7 of runner direction.Set each channel size as follows: the length of entrance channel 2
, the width of entrance channel 2
, the length of slit conduit 3
, the width of slit conduit 3
, the length of outlet flow 5
, the width of outlet flow 5
.
In the utility model, silicon crystal material is contained on slit conduit 3 surfaces.Take with the living radical legal system is standby the polyvinyl alcohol (PVA) that hydroxyl is terminal.The hydroxyl of polyelectrolyte brush 4 terminals and the silanol radical reaction on silicon wafer synthesize polyelectrolyte brush.Grafting density is got respectively
,
Representative does not have the polyelectrolyte brush effect.And applying uniform electric field 7 in the direction parallel with runner, electric-field intensity is
.Fluid particles 8 in slit conduit 3 comprises anion, cation and hydrone.Fig. 2 and Figure 3 shows that the schematic diagram of EOF each component in slit conduit 3 in micro-fluidic chip.
Figure 4 shows that grafting density is respectively
The time, slit conduit 3 fluid velocity curves in micro-fluidic chip, wherein electric-field intensity is
, speed unit is
,
For nominal time parameter in LJ unit; Figure 5 shows that grafting density is respectively
The time polyelectrolyte density curve, due to the flow passage structure symmetry, only show half runner height monomer density profile, wherein
For amount of monomer,
In system, the quantity of polymer monomer per unit volume, i.e. monomer density.
Known by the monomer density curve, polyelectrolyte brush 4, due to cation, applies direction along electric field and stretches gradually under electric field action.As seen in Figure 4, do not have in the slit conduit of grafting polyelectrolyte brush 4, fluid velocity obviously is greater than the speed of fluid in the slit conduit that is grafted with polyelectrolyte brush 4.Thereby proof, the water passage surface grafting is during with cationic polyelectrolyte brush 4, slit conduit 3 inner fluids will be subject to viscous friction effect and the electrostatic attraction effect of polyelectrolyte brush 4, and fluid velocity in slit conduit 3 is significantly reduced, and suppress the EOF effect obvious.As seen in Figure 5, under higher grafting density condition, polyelectrolyte brush 4, than lower grafting density, presents extended configuration.That is to say, along with the increase of grafting density, due to steric effect, stable flow velocity zone becomes relatively narrow, and the inhibitory action of EOF is strengthened gradually.
The foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, for a person skilled in the art, the utility model can have various modifications and variations.All any modifications that the utility model is done, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (8)
1. a micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush, it is characterized in that: comprise that the sample body enters unit, slit conduit unit, sample body deliverying unit and drives electric field, described sample body enters unit and comprises sample inlet pool (1) and entrance channel (2), and described entrance channel (2) two ends are connected with slit conduit (3) with sample inlet pool (1) respectively; Described slit conduit unit comprises slit conduit (3) and polyelectrolyte brush (4), described slit conduit (3) two ends are connected with outlet flow (5) with entrance channel (2) respectively, the water passage surface of described slit conduit (3) is with functional groups, can grafting polyelectrolyte brush (4), described polyelectrolyte brush (4) is with cation, and is grafted on the water passage surface of slit conduit (3) by physisorphtion or chemical bonding; Described sample body deliverying unit comprises outlet flow (5) and waste liquid pool (6), and described outlet flow (5) is connected with waste liquid pool (6) with slit conduit (3) respectively; Described driving electric field is the uniform electric field (7) applied being parallel to slit conduit (3) direction.
2. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, is characterized in that: the length of described entrance channel (2)
, width
.
3. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, is characterized in that: the length of described slit conduit (3)
, width
.
4. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, is characterized in that: the length of described outlet flow (5)
, the width of outlet flow (5)
.
5. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, it is characterized in that: described slit conduit (3) inner surface material is Graphene, silicon wafer, quartz, aluminium, gold, glass, natural rubber, carbon black, epoxy resin or dimethyl silicone polymer.
6. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, it is characterized in that: described polyelectrolyte brush (4) is with cationic polyelectrolyte brush, be specially poly 2 vinyl pyridine, butyl rubber, polyvinyl alcohol, polyvinyl acetate, crystal methamphetamine, acrylic resin, the polymethoxy ether, PEO, poly-sulfuration cyclopropane, poly-4-methoxyl group storax is rare, poly-2-methyl-1-propylene, poly-1, 3 dioxolanes, polyisobutene, Poly alpha Olefins (PAO), poly-alkyl vinyl ether, poly-trityl carbonium ion, polyacrylamide, PMAm, polyvinyl acetate, poly N-vinyl, poly-methyl oxygen base ethylacrylic acid or poly-hexadecyldimethyl benzyl ammonium ammonium chloride.
7. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, it is characterized in that: described polyelectrolyte brush (4) grafts on the grafting density on slit conduit (3) surface
.
8. the micro-fluidic chip that suppresses EOF at fluid channel surface grafting polyelectrolyte brush according to claim 1, is characterized in that: the intensity of described uniform electric field (7)
.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013202725317U CN203316140U (en) | 2013-05-20 | 2013-05-20 | Microfluidic chip grafted with polyelectrolyte brush on surface of micro-channel to inhibit electroosmotic flow |
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|---|---|---|---|
| CN2013202725317U CN203316140U (en) | 2013-05-20 | 2013-05-20 | Microfluidic chip grafted with polyelectrolyte brush on surface of micro-channel to inhibit electroosmotic flow |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103212457A (en) * | 2013-05-20 | 2013-07-24 | 吉林大学 | Micro-fluidic chip for inhibiting electroosmotic flows through grafting polyelectrolyte brush on surface of micro-channel |
| CN111936862A (en) * | 2019-03-11 | 2020-11-13 | 京东方科技集团股份有限公司 | Micro-channel and preparation method and operation method thereof |
-
2013
- 2013-05-20 CN CN2013202725317U patent/CN203316140U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103212457A (en) * | 2013-05-20 | 2013-07-24 | 吉林大学 | Micro-fluidic chip for inhibiting electroosmotic flows through grafting polyelectrolyte brush on surface of micro-channel |
| CN103212457B (en) * | 2013-05-20 | 2015-01-07 | 吉林大学 | Micro-fluidic chip for inhibiting electroosmotic flows through grafting polyelectrolyte brush on surface of micro-channel |
| CN111936862A (en) * | 2019-03-11 | 2020-11-13 | 京东方科技集团股份有限公司 | Micro-channel and preparation method and operation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20131204 Effective date of abandoning: 20150107 |
|
| RGAV | Abandon patent right to avoid regrant |