CN203269923U - PCR (polymerase chain reaction) workstation - Google Patents
PCR (polymerase chain reaction) workstation Download PDFInfo
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- CN203269923U CN203269923U CN 201320117255 CN201320117255U CN203269923U CN 203269923 U CN203269923 U CN 203269923U CN 201320117255 CN201320117255 CN 201320117255 CN 201320117255 U CN201320117255 U CN 201320117255U CN 203269923 U CN203269923 U CN 203269923U
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Abstract
The utility model discloses a PCR (polymerase chain reaction) workstation which comprises a poly-carbon transparent side wall (1), a working table (40), a filtering component (3) and a control component (11), wherein the side surfaces of the poly-carbon transparent side wall (1) are in radian crossing; the working table (40) is jointless whole stainless steel; the filtering component (3) is used for filtering the airflow entering the reaction workstation; and the control component (11) is used for receiving and controlling a sensor signal installed in the filtering component (3), carries out corresponding control action through a single chip and displays the result on a display (10). The PCR workstation disclosed by the utility model has the functions of ultraviolet irradiation, HEPA (high efficiency particulate air) filtration, ozone decomposition and ozone generation, and has the advantages of easiness in cleaning, real-time monitoring, safety and the like.
Description
Technical field
The utility model relates to the PCR workstation (platform) of a kind of PCR reaction experiment behind PCR reaction (polymerase chain reaction), particularly sample DNA template extraction in biomedicine and genetic field.
Background technology
PCR is called for short in polymerase chain reaction (Polymerase Chain Reaction), is a kind of Protocols in Molecular Biology, is used for amplifying specific DNA fragmentation.PCR has highly sensitive, and the miniprep dna function can increase.Yet if do not take correct safeguard procedures, its highly sensitive can make troubles equally.There is the investigator to think, not rigorous due to laboratory operation, the data in many gene order-checkings may be subject to the pollution of other DNA.In each laboratory, even significant care ground processing enters the sample in laboratory, amplification procedure all can produce a large amount of DNA.Reaction mixture an experiment has a copy DNA or other foreign DNAs, can be amplified, and causes reporting by mistake or wrong result.The DNA of this pollutent enters into reaction mixture by following approach usually:
1. contaminated test tube, feed head, pipettor;
2. when adding the DNA sample, bring into from air;
3. the partial reaction fluid component pollutes.
According to Ministry of Health of the People's Republic of China's " Clinic Gene Amplified check laboratory room managing tentative method " requirement, Clinic Gene Amplified checks the laboratory to be divided in principle four independent work areas: reagent is stored and area in preparation; Sample preparation district; Amplification reaction mixture preparation and amplification region; The amplified production analysis area.Generally all adopt the method for cleaning project in actual mechanical process, original laboratory is transformed.
Obviously, prior art pilot scale developing agent storage and area in preparation; Sample preparation district; Amplification reaction mixture preparation and amplification region; The amplified production analysis area; Change one's clothes, wind beautiful jade etc. all needs independently space.Therefore there are the inconveniences such as floor space is large, investment is high, use cost is high.
The utility model content
The utility model provides a kind of new polymerase chain reaction workstation for above-mentioned inconvenience.This workstation can prevent to greatest extent that PCR from polluting, reducing the infringement to operator.Adopt automatic control technology, detect in real time the parameters such as uitraviolet intensity, guarantee the workstation operational excellence.
A kind of polymerase chain reaction workstation is provided, comprises: the poly-transparent sidewall of carbon (1), intersection, side are that radian intersects;
Work top (40) is jointless monoblock stainless steel; Filter assemblies (3) is used for filtering the air that enters the polymerase chain reaction workstation; And control unit (11), be used for receiving the multiple sensors that controlled filter assembly (3) is installed, carry out data processing by the treater in control unit, result is presented on indicating meter (10), simultaneously the assembly that connects is controlled.
Further, in the workstation of described polymerase chain reaction, the transparent sidewall of described poly-carbon (1) is the poly-carbon solid slab of 3-10 mm thick.
Further, in the workstation of described polymerase chain reaction, described filter assemblies (3) comprises prefilter (31) in turn according to the direction of air flowing, blower fan (32), high efficiency filter (33); Prefilter (31) adopts filter core, is used for filtering the pollution substance of larger particles; Blower fan (32) drives the distinguished and admirable filtration unit that enters; High efficiency filter (33) is used for filtering more short grained pollution substance.
Further, in the workstation of described polymerase chain reaction, described prefilter adopts as lower filter or device, perhaps their combination:
Active carbon filter core, the airborne contaminant particles of filtering and unpleasant odor;
The ozone catalytic filter core, the ozone that the ozonizer in the filtering workstation produces and other unpleasant odor;
The hydroxide ion generating unit with the air ionization that enters in workstation, makes the better DNA deactivation of its performance.
Further, in the workstation of described polymerase chain reaction, fluorescent lamp (4) is installed in the transparent sidewall of described poly-carbon (1), is used for illumination; Filter assemblies (3) includes the dust particle that prefilter (31) be used for to filter 5 microns and above particle diameter, and high efficiency filter (33), filters the particulate matter that particle diameter equals 0.3 micron and above size.
Further, in the workstation of described polymerase chain reaction, described ultraviolet lamp (5) is UV C-band fluorescent tube, and this fluorescent tube emitting ultraviolet light is used for eliminating DNA and pollutes.
Further, in the workstation of described polymerase chain reaction, the described assembly that connects is controlled and comprised, cut-off ultraviolet power supply, drive warning howler, hummer, switch ozonizer, switch hydroxide ion producer and activated ozone catalytic specie.
By above-mentioned technique means, the utility model possesses following advantage:
1. the workstation vertical laminar flow air-flow that provides by prefilter and high efficiency filter (HEPA) in polymerase chain reaction provides the protection of ISO level Four neatness standard (be equivalent to u.s. federal standard 209E regulation 10 grades) for product.Use the HEPA high efficiency filter, for 0.3 μ m particle system up to 99.99% the efficient of damming;
2. workstation assembling once shaped, with the transparent sidewall of poly-carbon of radian, reduce to greatest extent the sterilization dead angle.Simultaneously, the ultraviolet ability of poly-carbon material tolerance is strong, can guarantee that outward appearance is constant.The transparent side window of the transparent poly-carbon of three sides increases lighting performance;
3. the safe front window of double-layer folding formula and ultraviolet circuit locking system have been guaranteed operator's safety;
4. operating table surface is durable and easy to clean monoblock stainless steel, without seam;
5. workstation assembles monolithic processor control device, UV sensor, differential pressure pickup, ozone sensor and negative ion sensor are installed, respectively the parameters such as negative ion that produce of the ozone concn that produces of the testing station UV-lamp of installing, HEPA two ends pressure reduction, ozonizer and photocatalysis apparatus.According to predefined each parameter index, analyze in real time judgement, respectively by station situations of reporting such as indicating meter, hummer and printers, remind operator to take corresponding measure, control simultaneously hydroxide ion producer, ozonizer and activated ozone catalyzer etc., also can store, record correlation parameter, in order to transfer later on use;
6. the pre-filtering layer of workstation is installed layer of active carbon filter core, the airborne contaminant particles of adsorbable, filtering and unpleasant odor;
7. workstation installation ultraviolet ray is shortwave (254nm) fluorescent tube, and light reflecting material is installed simultaneously, strengthens the ultraviolet function of killing, thereby eliminates the material that causes that easily DNA pollutes;
8.3-10 the transparent poly-carbon plate of mm thick is a kind of effective β radiation shielding, stops the β ray of P-32 isotropic substance emission, also plays a respiratory protection effect simultaneously, guarantees staff's safety.Preferably, can use the transparent poly-carbon plate of 5 mm thick.
Description of drawings
Accompanying drawing 1 is the side-view of polymerase chain reaction of the present utility model workstation.
Accompanying drawing 2 is the front view of polymerase chain reaction of the present utility model workstation.
Accompanying drawing 3 is the block diagram of the filter assemblies of polymerase chain reaction of the present utility model workstation.
Embodiment
Polymerase chain reaction of the present utility model workstation is intended to eliminate three main pollution channels.In " totally ", sealing, carry out response preparation and storage apparatus/consumptive material in relatively static wind box, thereby reduce air and source of supply contact scar.
Fig. 1 is the side-view of polymerase chain reaction of the present utility model workstation.This workstation mainly comprises: the poly-transparent sidewall 1 of carbon, work top 40, filter assemblies 3, and control unit 11.
Wherein poly-transparent sidewall 1 disposal molding of carbon, do not use traditional right angle intersection in the side, and adopt radian to intersect, and reduces to greatest extent the dead angle of sterilizing.Take the transparent side window of transparent poly-carbon at pros and cons and the left and right sides, increase lighting performance.But smear the antimicrobial coating anti-bacteria grows on the cabinet surface in the transparent sidewall of poly-carbon 1 inboard.Because the ultraviolet ability of poly-carbon material tolerance is strong, can guarantee that outward appearance is constant.The transparent poly-carbon plate of 3-10 mm thick is a kind of effective β radiation shielding, stops the β ray of P-32 isotropic substance emission, also plays a respiratory protection effect simultaneously, guarantees staff's safety.Preferably, can use the transparent poly-carbon plate of 5 mm thick.
The poly-transparent sidewall of carbon 1 front is equipped with safe front window and the ultraviolet interlocking system of double-layer folding formula, and the front apron hinge 12 as shown in Figure 1, and front apron ultraviolet ray switch 13 has been guaranteed operator's safety.
As shown in Figure 1, 2, in the poly-transparent sidewall 1 of carbon, fluorescent lamp 4 is installed, is used for illumination; Ultraviolet lamp 5 is installed, is used for sterilization.In the response preparation process, the reaction mixture of part is exposed under UV light, thereby purifies the contaminated reactive component of any possibility.Due to this rayed whole space be placed on wherein article, they can be at one time by " cleaning ".By keeping the Qianmen at blocking, enclosed chamber and articles for use are in the state of being ready to, in order to using next time.
Described ultraviolet lamp 5 is UV C-band fluorescent tube, be the 254nm fluorescent tube, this fluorescent tube emitting ultraviolet light contiguous thymus pyrimidine (Thymine) base in the microbial DNA chain mutually combines it, cause its breeding of can not regenerating, thereby play the effect that DNA pollutes of eliminating.
According to being equipped with in field condition filter assemblies 3 as one of lower device or their combination (not shown): UV sensor, differential pressure pickup, ozone sensor and negative ion sensor, ultraviolet power supply, warning howler, hummer, ozonizer, hydroxide ion producer and ozone catalytic material.Said apparatus is connected to control unit, and by its control.
Active carbon filter core, the airborne contaminant particles of filtering and unpleasant odor;
Ozone catalytic filter filter core, the ozone that the ozonizer in the filtering workstation produces and other unpleasant odor.Have heating unit in this ozone catalytic filter core, can on-line heating to 350 degree centigrade and more than so that periodic deactivated catalyst and remove ozone.Described ozone catalytic filter material is metal oxide containing precious metals or its mixture.
The hydroxide ion generating unit is with the air ionization that enters in workstation, in order to bring into play the effects such as important oxidation sterilizing, sterilization.
With reference to Fig. 4, control unit 11 mainly comprises the micro-chip element, control UV sensor, differential pressure pickup, ozone sensor and the negative ion sensor install, respectively the parameters such as negative ion that produce of the ozone concn that produces of UV-lamp, filter assemblies 3 two ends differential pressure of atmospheric pressure, the ozonizer installed of testing station and photocatalysis apparatus.According to predefined each parameter index, analyze in real time judgement, respectively by station situations of reporting such as indicating meter 10, hummer and printers, remind operator to take corresponding measure.Simultaneously, also can store, record correlation parameter, in order to transfer later on use.The basic function of this micro-chip as shown in Figure 4.
Polymerase chain reaction provided by the utility model workstation is not limited to PCR experiment, also can be used between clean work station, Biohazard Safety Equipment, isolation, aseptic workplace, bottling department, cleaning project and healthy relevant industries fields etc. need to need to control and detect Anywhere air and the index such as aseptic.
Claims (7)
1. polymerase chain reaction workstation comprises:
The poly-transparent sidewall of carbon (1), intersection, side are that radian intersects;
Work top (40) is jointless monoblock stainless steel;
Filter assemblies (3) is used for filtering the air that enters the polymerase chain reaction workstation;
And control unit (11), be used for receiving the multiple sensors that controlled filter assembly (3) is installed, carry out data processing by the treater in control unit, result is presented on indicating meter (10), simultaneously the assembly that connects is controlled.
2. polymerase chain reaction as claimed in claim 1 workstation, it is characterized in that: the transparent sidewall of described poly-carbon (1) is the poly-carbon solid slab of 3-10 mm thick.
3. polymerase chain reaction as claimed in claim 1 workstation, it is characterized in that: described filter assemblies (3) comprises prefilter (31) in turn according to the direction of air flowing, blower fan (32), high efficiency filter (33); Prefilter (31) adopts filter core, is used for filtering the pollution substance of larger particles; Blower fan (32) drives the distinguished and admirable filtration unit that enters; High efficiency filter (33) is used for filtering more short grained pollution substance.
4. polymerase chain reaction as claimed in claim 3 workstation, it is characterized in that: described prefilter (31) adopts as lower filter or device, perhaps their combination:
Active carbon filter core, the airborne contaminant particles of filtering and unpleasant odor;
The ozone catalytic filter core, the ozone that the ultraviolet ray in the filtering workstation produces;
The hydroxide ion generating unit with the air ionization that enters in workstation, makes the better DNA deactivation of its performance.
5. polymerase chain reaction as claimed in claim 1 workstation, is characterized in that: fluorescent lamp (4) is installed in the transparent sidewall of described poly-carbon (1), is used for illumination; Filter assemblies (3) includes the dust particle that prefilter (31) be used for to filter 5 microns and above particle diameter, and high efficiency filter (33), filters the particulate matter that particle diameter equals 0.3 micron and above size.
6. polymerase chain reaction as claimed in claim 5 workstation, it is characterized in that: it is UVC wave band fluorescent tube that ultraviolet lamp (5) is installed in the transparent sidewall of described poly-carbon (1), this fluorescent tube emitting ultraviolet light is used for eliminating DNA and pollutes.
7. polymerase chain reaction as claimed in claim 1 workstation, it is characterized in that: the described assembly that connects is controlled comprised, cut-off ultraviolet power supply, drive warning howler, hummer, switch ozonizer, switch photocatalysis apparatus and activated ozone catalytic specie.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320117255 CN203269923U (en) | 2013-03-15 | 2013-03-15 | PCR (polymerase chain reaction) workstation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320117255 CN203269923U (en) | 2013-03-15 | 2013-03-15 | PCR (polymerase chain reaction) workstation |
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| CN203269923U true CN203269923U (en) | 2013-11-06 |
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| CN 201320117255 Expired - Lifetime CN203269923U (en) | 2013-03-15 | 2013-03-15 | PCR (polymerase chain reaction) workstation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695309A (en) * | 2013-12-19 | 2014-04-02 | 江苏金太生命科技有限公司 | Novel PCR (Polymerase Chain Reaction) instrument |
| CN104893951A (en) * | 2014-03-03 | 2015-09-09 | 北京双诚联盈净化工程技术有限公司 | Combination of polymerase chain reaction workstation |
| CN105772135A (en) * | 2016-04-13 | 2016-07-20 | 合肥九源环境科技有限公司 | Clean bench |
| CN108603155A (en) * | 2016-09-23 | 2018-09-28 | 深圳华大智造科技有限公司 | A kind of system being used to prepare gene sequencing sample and its application |
-
2013
- 2013-03-15 CN CN 201320117255 patent/CN203269923U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695309A (en) * | 2013-12-19 | 2014-04-02 | 江苏金太生命科技有限公司 | Novel PCR (Polymerase Chain Reaction) instrument |
| CN104893951A (en) * | 2014-03-03 | 2015-09-09 | 北京双诚联盈净化工程技术有限公司 | Combination of polymerase chain reaction workstation |
| CN104893951B (en) * | 2014-03-03 | 2019-03-05 | 北京双诚联盈净化工程技术有限公司 | The combination of polymerase chain reaction work station |
| CN105772135A (en) * | 2016-04-13 | 2016-07-20 | 合肥九源环境科技有限公司 | Clean bench |
| CN108603155A (en) * | 2016-09-23 | 2018-09-28 | 深圳华大智造科技有限公司 | A kind of system being used to prepare gene sequencing sample and its application |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131106 |
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| CX01 | Expiry of patent term |