CN203249923U - Fluorescent quantitative detecting system for furaltadone metabolite - Google Patents
Fluorescent quantitative detecting system for furaltadone metabolite Download PDFInfo
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- CN203249923U CN203249923U CN 201220710349 CN201220710349U CN203249923U CN 203249923 U CN203249923 U CN 203249923U CN 201220710349 CN201220710349 CN 201220710349 CN 201220710349 U CN201220710349 U CN 201220710349U CN 203249923 U CN203249923 U CN 203249923U
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- YVQVOQKFMFRVGR-VGOFMYFVSA-N 5-(morpholin-4-ylmethyl)-3-[(e)-(5-nitrofuran-2-yl)methylideneamino]-1,3-oxazolidin-2-one Chemical class O1C([N+](=O)[O-])=CC=C1\C=N\N1C(=O)OC(CN2CCOCC2)C1 YVQVOQKFMFRVGR-VGOFMYFVSA-N 0.000 title abstract description 22
- TVHAMVOINIHMEX-UHFFFAOYSA-N 3-amino-5-morpholinomethyl-2-oxazolidinone Chemical compound O1C(=O)N(N)CC1CN1CCOCC1 TVHAMVOINIHMEX-UHFFFAOYSA-N 0.000 claims abstract description 78
- 238000001514 detection method Methods 0.000 claims abstract description 54
- 238000012360 testing method Methods 0.000 claims abstract description 52
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- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical class CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 1
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- NXFQHRVNIOXGAQ-YCRREMRBSA-N nitrofurantoin Chemical compound O1C([N+](=O)[O-])=CC=C1\C=N\N1C(=O)NC(=O)C1 NXFQHRVNIOXGAQ-YCRREMRBSA-N 0.000 description 1
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Images
Abstract
The utility model discloses a fluorescent quantitative detecting system for a furaltadone metabolite. The system comprises a furaltadone metabolite fluorescent quantitative detection immunochromatography kit and a fluorescent quantitative detector, wherein the kit comprises a test strip and a fluorescence labeling solution; the a detecting area of the test strip is coated with an AMOZ-BSA conjugate, and a quality control area is coated with an IgG antibody; and the fluorescence labeling solution contains a fluorescence labeling AMOZ antibody and a fluorescence labeling mouse IgG antibody. The system is matched with the fluorescent quantitative detector in the system and used for detecting the concentration of the furaltadone metabolite in a sample. The test strip in the detecting system is compared with an immuno gold staining labeling test strip, and the sensitivity is higher; compared with an enzyme-linked method, the operation is rapider, simpler and more convenient; and the system is matched with the fluorescent quantitative detector, and accurate quantitative of furaltadone can be realized.
Description
Technical field
The utility model belongs to field of medical examination, and specifically, the utility model relates to a kind of AMOZ fluorescent quantitation detection system, comprises that the AMOZ fluorescent quantitation detects immunochromatographytest test kit and fluorescent quantitative detector.
Background technology
Furaltadone belongs to a kind of in the itrofurans medicine, and the itrofurans medicine is the broad spectrum antibiotic of a class chemosynthesis, mainly comprises furaltadone, furazolidone, nitrofurazone and furantoin.Such medicine 5～10mg/kg can suppress multiple Gram-positive and negative bacterium, and 20～50mg/kg has bactericidal action, and some protozoon, fungi are also had certain effect.Main Function suppresses acetyl coenzyme A in the microbial enzyme system, disturbs the microorganism carbohydate metabolism, thereby plays effect antibacterial, sterilization.Its antimicrbial power is not subjected to blood, ight soil, pus and organizes decomposition product to affect, and external application is little to tissue irritation, and bacterium is to also less generation drug resistance of this class medicine.Thereby once be widely used in treating Animal diseases, and because it has somatotrophic function, also once used as feed addictive.Itrofurans medicine in animal body metabolism is rapid, drug half-life approximately 5 hours, AMOZ AMOZ, chemical name is 5-methyl morpholine-3-amino-2-oxazolidone, can combine closely with epicyte protein and steady in a long-term the existence, so that release rate is slack-off in vivo for medicine, and the food-processing method that daily life is common all is difficult to make the metabolin of protein combination attitude to be degraded in a large number such as microwave processing, the cooking etc.And after the food that contains metabolin was eaten by the mankind, these metabolins will be hydrolyzed from protein and out be absorbed by the body under the acid condition of gastric juice.Yet, studies show that, heavy dose of or use for a long time the itrofurans medicine and all can produce toxic action to livestock and poultry, and such medicine also has carcinogenic teratogenesis mutagenesis, and furaltadone is strong carcinogenicity medicine especially.European Union's regulations of promulgating nineteen ninety are classified itrofurans medicine and metabolin thereof as category-A forbidding veterinary drug, stipulate that its residue detection in animal derived food is limited to 1.0ug/kg, nineteen ninety-five rises and forbids in the consumption animal using such medicine, is defined in detecting in the animal derived food and must not be limited to and detect.Subsequently, the country such as the U.S. and China has also prohibited the use of such medicine.Japan limited the quantity of nitrofuran and metabolite residue thereof in 2006 and reduces to 0.5ug/kg.In recent years, the China's export product is frequently found, and the itrofurans medicine is residual, and this has not only caused serious economic loss, has also affected the international image of China.Above example has all caused serious economic loss.Change the present situation of animal food safety, primarily improve people to the understanding of animal food safety problem, from the theory and technology angle, setting up suitable residue of veterinary drug analytical approach and formulating residue criterion is the most basic aspect.
Main method for detection of itrofurans medicine and metabolite residue thereof in the animal derived food has liquid chromatography-UV-detector method (LC-UV), tablets by HPLC-MS (HPLC-MS), High Performance Liquid Chromatography/Mass Spectrometry/MS (HPLC-MS/MS) at present, advantages such as although these methods have accurately and reliably, repeatability is strong, instrument costliness, complicated operation, portable difference etc. are unfavorable for applying on a large scale and high flux detects.Therefore, this patent is set up a kind of easy, reliable, quick, detection method that Sensitive Detection AMOZ is residual, in order to monitor the AMOZ in the animal tissue is residual.
Application number is the patent of invention of CN 201020586590, discloses a kind of ELISA detection kit that detects AMOZ in the aquatic products.This kit forms and comprises: 96 hole ELISA Plate, ELIAS secondary antibody, antibody working fluid, standard solution, substrate nitrite ion, stop buffer, concentrated cleaning solution, concentrated redissolution liquid, 2-nitrobenzaldehyde, cover plate film, valve bag, instructions and quality inspection report.Adopt the indirect competitive ELISA method, pre-coated coupled antigen on the ELISA Plate capillary strip, AMOZ residual in the sample will be competed anti-AMOZ antibody with pre-coated coupled antigen on the capillary strip, after adding ELIAS secondary antibody, develop the color with tmb substrate, the sample absorbance multiply by its corresponding extension rate more again with typical curve, can draw the residual quantity of AMOZ in the sample.Compare with instrument analysis technology and to have the characteristics such as easy and simple to handle, that expense is cheap, sensitivity is high.But this method is unfavorable for on-the-spot test.
The utility model content
The purpose of this utility model is to overcome the defective of above-mentioned prior art, provide a kind of easy and simple to handle, sensitivity is strong, quantitatively accurately, the fast AMOZ fluorescent quantitation of detection speed detects detection system, comprise that a kind of AMOZ fluorescent quantitation detects immune chromatography reagent kit, fluorescent quantitative detector in the coupled system uses, and can realize the accurate quantification to furaltadone.
For achieving the above object, the utility model has been taked following technical scheme:
AMOZ fluorescent quantitation detection system comprises that the AMOZ fluorescent quantitation detects immune chromatography reagent kit and fluorescent quantitative detector.Described kit comprises that the AMOZ fluorescent quantitation detects immuno-chromatographic test paper strip and fluorescence labeling liquid; Described fluorescent quantitative detector comprises fluorescence light source system and fluorescence detecting system.
Wherein, described AMOZ fluorescent quantitation detects immune chromatography reagent kit, comprises test strips and fluorescence labeling liquid, and described test strips is overlapped in turn to stick on the base plate by sample pad, cellulose nitrate coated film, thieving paper and consists of; Described cellulose nitrate coated film comprises detection zone and Quality Control district, and described detection zone is coated with AMOZ AMOZ antigen; Described Quality Control district is coated with dynamics; Contain fluorescence labeling AMOZ antibody and fluorescence labeling mouse IgG antibody in the described fluorescence labeling liquid.
The excitation wavelength of described fluorescence labeling liquid (Ex) is 310-550nm, and emission wavelength (Em) is 340-620nm.
Described AMOZ fluorescent quantitation detects immune chromatography reagent kit, also include the card shell, described test strips is packaged in the card shell, the surface of described card shell one side is provided with well and display window, described well is corresponding to the position of test strips sample pad, and described display window is corresponding to the detection zone of test strips and the position in Quality Control district.
The shell of the immune chromatography reagent kit of described fluorescent quantitation detection AMOZ also scribbles the coding table basic unit for the product information of fluorescent scanning identification.
The detection principle that fluorescent quantitation described in the utility model detects the immunity-chromatography test carton of AMOZ is competition law, fluorescein molecule or fluorescent latex particulate and AMOZ antibody covalent bond.To detect sample (urine sample or extract) and add in the fluorescent marker, its fluorescence labeling AMOZ antibody can be combined by the AMOZ in urine, forms compound; And the AMOZ-BSA conjugate that is coated on detection zone on the nitrocellulose filter (T) is also competed combined with fluorescent mark AMOZ antibody.After containing the AMOZ sample and fluorescent marker mixes, drop on the test strips, mixed liquor moves forward along nitrocellulose filter under the chromatography effect, contained AMOZ amount is more in the sample, the fluorescently-labeled antibody that can be combined with T district AMOZ-BSA conjugate is fewer, reduces thereby make the T district record the fluorescence value of detecting.By fluorescence detector scanning T district fluorescence signal intensity, can detect AMOZ content in the sample.
AMOZ immuno-chromatographic test paper strip of the present utility model is exempted from method detection furaltadone with GC/MS, HPLC isochromatic spectrum instrument and enzyme and is compared, and has easy (one step of simple operations finishes), is fit to varying number pattern detection and quick advantages such as (about 15 minutes the result can be arranged); Compare with the immuno-gold labeling test strips, the utlity model has the advantages such as sensitivity is higher, accurate quantitative analysis.
Fluorescent quantitation of the present utility model detects the AMOZ immune chromatography reagent kit, the method that adopts fluorescence labeling liquid and sample to be pre-mixed, make reaction and signal discharge more homogeneous, compare with other chromatography, the preci-sion and accuracy of its batch production reaches best effects.
Secondly, the utility model provides a kind of fluorescent quantitative detector, this detector comprises excitation source module, photoelectric conversion module, control analysis module and software systems, the fluorescence signal that sends by detection zone on the test strip carries out the result and reads, and can carry out sensitive quantitative measurement to AMOZ AMOZ within 10 seconds.
Furaltadone fluorescent quantitation detection system provided by the utility model can faster more accurately be measured the residual quantity of contained AMOZ AMOZ in the animal tissue, has accuracy good (recovery is 80%-110%), quantitative deviation little (in 20%), highly sensitive (sensitivity reaches 0.3ug/kg), the sample size few (80ul) that needs, the advantage such as easy and simple to handle.
Description of drawings
Fig. 1 is the structural representation that described fluorescent quantitation of the present utility model detects test strips in the AMOZ chromatography kit;
Fig. 2 is the structural representation that described fluorescent quantitation of the present utility model detects test strips in the AMOZ chromatography kit;
Fig. 3 is that described fluorescent quantitation of the present utility model detects the structural representation that is used for the test card of placement test strips in the AMOZ chromatography kit;
Fig. 4 is the stereographic map of fluorescent quantitative detector described in the utility model.
Description of reference numerals:
Sample pad, 2, the cellulose nitrate coated film, 3, coated detection zone, 4, the Quality Control district, 5, suction, 6, base plate, 7, the card shell, 8, display window, 9, well.
Embodiment
Fluorescent quantitation in the utility model AMOZ fluorescent quantitation detection system detects the furaltadone immune chromatography reagent kit, and to adopt fluorescence labeling liquid be the label of fluorescein and albumen or with the label of fluorescent latex and albumen, wherein employed fluorescein is wherein one or more such as fluorescein isothiocynate, RB 200, TRITC, phycoerythrin, the green fibroin of many dinoflagellates, lanthanide chelate, Fluoresceincarboxylic acid.
In the utility model embodiment, the AMOZ antibody that adopts is the monoclonal antibody of conventional monoclonal antibody technique preparation, and the AMOZ AMOZ antigen that adopts is to utilize the conventional chemical synthetic method to obtain, and utilizes the competition ratio juris to detect sample.
Test strips in the utility model is according to the conventional method preparation of this area, and the width of test strips is common 2.7-3.5mm, and the width of the test strips that following examples exemplify is 3mm.
The fluorescence detector that the utility model is used be adopt that Guangzhou ten thousand inspires confidence in biotechnology incorporated company independent development fly to survey fluorescence detector (model: WF-0901/1).
Describe the utility model in detail below in conjunction with the drawings and specific embodiments.
Embodiment one
In this embodiment, furaltadone fluorescent quantitation detection system comprises that fluorescent quantitation detects AMOZ immune chromatography reagent kit and fluorescent quantitative detector.
Described AMOZ fluorescent quantitation detects immune chromatography reagent kit and includes test strips and fluorescence labeling liquid.
Wherein, conventional method by test strips, test strips consists of successively mutually overlap joint ground stickup by sample pad 1, the cellulose nitrate coated film 2 that comprises detection zone (T district) 3 and Quality Control district (C district) 4, thieving paper 5 at base plate 6 and forms, as depicted in figs. 1 and 2.
In this embodiment, the detection zone T line place of coated film 0.2mg/ml AMOZ-BSA conjugate (AMOZ AMOZ antigen) coating buffer, use amount is 90ul/27cm.Be that the dynamics of 0.2mg/ml is coated with at the Quality Control district C of coated film line place working concentration, use amount is all 90ul/27cm.The mouse IgG antibody that is used for the combined with fluorescent mark is for detection of the validity of test strips.
In this embodiment, fluorescence labeling liquid is excited by 310nm, and emission wavelength is 340nm.In this embodiment, the preparation method (A) of carboxylic fluorescent latex label (the present embodiment use fluorescein be umbelliferone latex) is adopted in the preparation of fluorescence labeling liquid, and step is as follows:
With the umbelliferone latex of 100mg respectively with after 14mg AMOZ antibody or 14mg mouse IgG antibody mix, slowly add while stirring 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides (EDC) and N-hydroxy-succinamide (NHS), the content that makes EDC in its whole reaction system is 0.2mg, NHS content is 0.1mg, and 2～8 ℃ of lucifuge reactions are spent the night at low temperatures.Remove impurity with dialysis or additive method, redissolve with the fluorescence protective agent.
AMOZ antibody and the fluorescent latex particulate mark mouse IgG antibody of the above-mentioned fluorescent latex particulate mark for preparing are mixed by proper proportion, so that two kinds of antibody concentration are 0.2ug/ml all respectively, packing is for subsequent use.
Secondly, the utility model furaltadone fluorescent quantitation detection system also comprises fluorescent quantitative detector, comprise fluorescence light source system, detection system and automatic software analysis and Control system, can carry out to the fluorescence labeling furaltadone in the ELISA test strip district that has added on request sample fluorescence signal and process, read and the output detections result.
Embodiment two
In this embodiment, furaltadone fluorescent quantitation detection system comprises that the AMOZ fluorescent quantitation detects immune chromatography reagent kit and fluorescent quantitative detector.
At first, described AMOZ fluorescent quantitation detects immune chromatography reagent kit and includes test strips and fluorescence labeling liquid.
Wherein, test strips is sticked on the base plate by the conventional method of test strips successively mutually by sample pad, the cellulose coated film that comprises detection zone (T district) and Quality Control district (C district), thieving paper overlap joint.
In this embodiment, the detection zone T line place of coated film 1.2mg/ml AMOZ-BSA conjugate (AMOZ AMOZ antigen) coating buffer, use amount is 90ul/35cm.Be that the dynamics of 0.5mg/ml is coated with at the Quality Control district C of coated film line place working concentration, use amount is all 90ul/35cm, is used for the mouse IgG antibody of combined with fluorescent mark, for detection of the validity of test strips.
In this embodiment, after fluorescence labeling liquid was excited by 550nm, emission wavelength was 620nm.In this embodiment, the preparation method contain amino fluorescent latex label (the present embodiment use fluorescein be TRITC latex) is adopted in the preparation of fluorescence labeling liquid, and step is as follows:
100mg fluorescent latex label respectively with after 20mg AMOZ antibody or 20mg mouse IgG antibody mix, is placed 4～40 ℃ of environment, and regulation system pH7.0～8.5 slowly add 0.4～0.6% glutaraldehyde while stir; Reacted 2～5 hours, dialysis or additive method are removed impurity, redissolve with the fluorescence protective agent.
AMOZ antibody and the fluorescent latex particulate mark mouse IgG antibody of the above-mentioned fluorescent latex particulate mark for preparing are mixed by proper proportion, and consequently two kinds of antibody concentration are 0.5ug/ml all respectively, and packing is for subsequent use.
Secondly, the utility model furaltadone fluorescent quantitation detection system also comprises fluorescent quantitative detector, comprise fluorescence light source system, detection system and automatic software analysis and Control system, can carry out to the fluorescence labeling furaltadone in the ELISA test strip district that has added on request sample fluorescence signal and process, read and the output detections result.
Embodiment three
In this embodiment, furaltadone fluorescent quantitation detection system comprises that the AMOZ fluorescent quantitation detects immune chromatography reagent kit and fluorescent quantitative detector.
At first, described AMOZ fluorescent quantitation detects immune chromatography reagent kit and includes test strips and fluorescence labeling liquid.
Wherein, test strips is sticked on the base plate, by the conventional method of test strips successively mutually by sample pad, the cellulose coated film that comprises detection zone (T district) and Quality Control district (C district), thieving paper as depicted in figs. 1 and 2 overlap joint.
In this embodiment, the detection zone T line place of coated film 1.0mg/ml AMOZ-BSA conjugate (AMOZ AMOZ antigen) coating buffer, use amount is 90ul/35cm.Be that the dynamics of 0.5mg/ml is coated with at the Quality Control district C of coated film line place working concentration, use amount is all 90ul/35cm.The mouse IgG antibody that is used for the combined with fluorescent mark is for detection of the validity of test strips.
[0048] in this embodiment, fluorescence labeling liquid is excited by 490nm, and emission wavelength is 530nm; Simultaneously, the preparation method of the fluorescein (the present embodiment use fluorescein be fluorescein isothiocynate) of sulfur-bearing carbon acylamino is adopted in the preparation of fluorescence labeling liquid, and step is as follows:
Dissolve respectively 20mg AMOZ antibody or 20mg mouse IgG antibody with pH8.0～pH9.6 carbonic acid buffer, with the pH8.0～dissolving of pH9.6 carbonic acid buffer or suspension 3.5mg fluorescein isothiocynate; While stirring above-mentioned fluorescein isothiocynate is added in the globulin solution gradually, after adding, continue lucifuge and stir about 12h, after complete, in the bag filter of packing into, with above-mentioned carbonic acid buffer saline dialysed overnight at low temperatures, with the redissolution of fluorescence protective agent.
AMOZ antibody and the fluorescent latex particulate mark mouse IgG antibody of the above-mentioned fluorescent-substance markers for preparing are mixed by proper proportion, and consequently two kinds of antibody concentration are 1ug/ml all respectively, and packing is for subsequent use.
Secondly, the utility model furaltadone fluorescent quantitation detection system also comprises fluorescent quantitative detector, comprise fluorescence light source system, detection system and automatic software analysis and Control system, can carry out to the fluorescence labeling furaltadone in the ELISA test strip district that has added on request sample fluorescence signal and process, read and the output detections result.
Fluorescent quantitation described in the utility model furaltadone fluorescent quantitation detection system detects the AMOZ immune chromatography reagent kit, in instantiation, semi-manufacture assemble by following operation: by sample pad, coated film, thieving paper overlaps in turn and sticks on the base plate, consist of test strips, can be again with card shell 7 of the prior art (as shown in Figure 3), be fixed into test card, described card shell scribbles can be for the product information coding of luminoscope scanning recognition, ID chip (the quantitative computing formula that ID chip of the prior art adopts is semilog straight line equation or other calculation equations of detected signal value and calculating concentration, can automatically carry out the result and judge) with writing information, divide fluorescence labeling liquid and other accessories of installing to be assembled into kit.
Fluorescent quantitation described in the utility model detects the immune chromatography reagent kit of AMOZ, in use, be assembled in by plastics upper casing and plastics lower casing and fasten in the plastic clip shell (test card) that forms, the plastics upper casing is provided with two perforates, well 9 and display window 8, well 9 detects the immuno-chromatographic test paper strip sample pad of AMOZ corresponding to described fluorescent quantitation, display window 8 detects detection zone and the Quality Control district of the immuno-chromatographic test paper strip of AMOZ corresponding to described fluorescent quantitation as a result, and the immuno-chromatographic test paper strip that this fluorescent quantitation detects AMOZ can take out from this plastic casing.
Be used for testing the fluorescent quantitative detector of AMOZ in the utility model, mainly comprise fluorescence light source system, detection system and automatic software analysis and Control system.
Among the embodiment of the present utility model, detecting sample need to be by following operation: draw sample extraction liquid and the fluorescence labeling liquid mixed in equal amounts of 50～150ul, draw 50～150ul behind the mixing, past horizontal positioned test card well adding, avoid bringing into bubble, the reaction of beginning chromatography.Reacted 15 minutes, by fluorescent quantitative detector read test result.
The described AMOZ fluorescent quantitation of embodiment 1-3 of the present utility model detection system comparison shows that to the measurement result of 200 routine sample extraction liquid: the accuracy of measuring AMOZ in 0.2ppb～10ppb scope is high: quantitatively the curve linear coefficient is all greater than 0.99, its accuracy of AMOZ that contains 1ppb and 2ppb concentration in the sample is 80%～120%, and kit detects and is limited to 0.3ppb.The relatively general colloidal gold method (qualitative) that adopts and the testing result of enzyme linked immunosorbent detection method detection kit have better sensitivity and specificity, table specific as follows:
More than be specifying for possible embodiments of the present utility model, but this embodiment limits claim of the present utility model, allly do not break away from the equivalence that the utility model skill spirit does and implement or change, all should be contained in the claim of the present utility model.
Claims (3)
1. AMOZ fluorescent quantitation detection system comprises that the AMOZ fluorescent quantitation detects immune chromatography reagent kit and fluorescent quantitative detector; Described kit comprises that the AMOZ fluorescent quantitation detects immuno-chromatographic test paper strip and fluorescence labeling liquid; Described fluorescent quantitative detector comprises fluorescence light source system and fluorescence detecting system.
2. AMOZ fluorescent quantitation detection system according to claim 1, described AMOZ fluorescent quantitation detects immune chromatography reagent kit, comprise test strips and fluorescence labeling liquid, described test strips is overlapped in turn to stick on the base plate by sample pad, cellulose nitrate coated film, thieving paper and consists of; Described cellulose nitrate coated film comprises detection zone and Quality Control district, and described detection zone is coated with AMOZ AMOZ antigen; Described Quality Control district is coated with dynamics.
3. AMOZ fluorescent quantitation detection system according to claim 2 is characterized in that, the excitation wavelength of described fluorescence labeling liquid is 310～550nm, and emission wavelength is 340～620nm.
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| CN 201220710349 CN203249923U (en) | 2012-12-19 | 2012-12-19 | Fluorescent quantitative detecting system for furaltadone metabolite |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201220710349 CN203249923U (en) | 2012-12-19 | 2012-12-19 | Fluorescent quantitative detecting system for furaltadone metabolite |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103698519A (en) * | 2012-09-28 | 2014-04-02 | 北京勤邦生物技术有限公司 | Chemiluminescence detection kit for furaltadone metabolite and applications of the kit |
| CN108663505A (en) * | 2018-06-08 | 2018-10-16 | 中国科学院上海生命科学研究院湖州营养与健康产业创新中心 | The remaining kits of aflatoxins M1 and preparation method in a kind of detection dairy produce |
| CN110646616A (en) * | 2019-09-05 | 2020-01-03 | 桂林理工大学 | Hypersensitivity fluorescence quenching immunosensor for detecting human cTnI in serum and detection method |
-
2012
- 2012-12-19 CN CN 201220710349 patent/CN203249923U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103698519A (en) * | 2012-09-28 | 2014-04-02 | 北京勤邦生物技术有限公司 | Chemiluminescence detection kit for furaltadone metabolite and applications of the kit |
| CN103698519B (en) * | 2012-09-28 | 2016-04-20 | 北京勤邦生物技术有限公司 | A kind of chemiluminescence detection kit of AMOZ and application thereof |
| CN108663505A (en) * | 2018-06-08 | 2018-10-16 | 中国科学院上海生命科学研究院湖州营养与健康产业创新中心 | The remaining kits of aflatoxins M1 and preparation method in a kind of detection dairy produce |
| CN110646616A (en) * | 2019-09-05 | 2020-01-03 | 桂林理工大学 | Hypersensitivity fluorescence quenching immunosensor for detecting human cTnI in serum and detection method |
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