CN2032446U

CN2032446U - Intelligent corpuscle detector

Info

Publication number: CN2032446U
Application number: CN 88211331
Authority: CN
Inventors: 董志澄; 万嘉若; 鲍贤方; 陈伟杰; 翁庆丰
Original assignee: East China Normal University
Current assignee: East China Normal University; Donghua University
Priority date: 1988-01-12
Filing date: 1988-01-12
Publication date: 1989-02-15

Abstract

The utility model relates to an intelligent corpuscle detector which belongs to a biological medicine measuring instrument. The utility model can be used for physic clinical diagnosis and be used for measuring the number of red blood cells, the white blood cells and the blood platelets, and the average volume and specific volume of red blood cell. The utility model can be used for measuring the bacterial number in the microbe domain and measuring the particle number and the particle area in other domains. The intelligent corpuscle detector is composed of a microscope, a television camera, a display, a host machine of the detecting instrument, etc. By the slide counting technique, the image of particles carries out data processing under the control of a microprocessor, and in split second (scores of milliseconds) the processed results can be displayed on a nixie display indicator and can be printed by a printer.

Description

Intelligent corpuscle detector

Intelligent particle detector, belong to the biomedical measurement instrument, can be used for medical clinic applications, measure RBC number, leukocyte count, platelet count, mean corpuscular volume, hematid specific volume, also can be used for microorganism field and measure bacterial population and other field mensuration particle number and microgranule sectional area etc.

At medical domain,, adopt slide counting and two kinds of technology of micropore counting usually to countings such as erythrocyte, leukocyte, platelet.Classical slide counting technology realized by manual operations, though advantage such as it has pair environmental requirement not high, and is easy and simple to handle often is subjected to the former of the aspect of operator own thereby influences the correctness of its mensuration.The micropore counting technology is realized by computer control photoelectric technology; can once finish erythrocyte, leukocyte, enumeration of thrombocytes; but it is very strict to conditional requests such as operating environment, reagent; having dust to sneak into slightly is cisco unity malfunction; sometimes can influence the correctness of counting again simultaneously because of several microgranules (cell) by micropore; clogging also appears easily; and as the Coulter instrument of Britain; when count value is higher; (for example leukocyte surpasses about 200,000), just, speed can't not count because of catching up with.Measure hematid specific volume, usually adopt centrifuging, per minute rotating speed 3600 times, need just can measure the result half an hour, FDAC board MC201 instrument per minute rotating speed 12000 times also needs 15 minutes, and along with faster rotational speed, temperature raises and influences the variation of red cell morphology, thereby influences the correctness of testing result.After the computer technology extensive use, the slide counting technology that development is classical combines it and micro tv image processing system.External close equipment is the big system of measuring multiple parameters, does not find to be used for blood routine examination.Domestic close equipment is XJ-1 type platelet, blood counting instrument, and because of adopting the logic circuit technical limitations, its count value need be handled in the enterprising row operation of computer, uses inconvenience, and can not satisfy the measurement of the whole indexs of routine blood test.

The purpose of this utility model provides a kind of multi-functional particle detection.This is a kind of by microscope 2, TV camera 1, the particle detection that the detection main frame 3 of single board computer control and display 4 constitute.This device had both improved the accuracy rate of slide counting, did not need the strict like that environmental condition of micropore counting again, and measuring techniques such as microgranule (cell) counting, area, specific volumes comprehensively in a small-sized instrument.Can satisfy the whole index measurements of medical clinic applications routine blood test.Because computer adopts single board computer, has both reduced cost, help clinical medicine and use and study, can change and expand its function again, the other field beyond biomedicine is used.

The The general frame of this detector is Fig. 1.Its measuring principle is: by microscope 2 and gamma camera 1 cell image of taking a sample on the slide is converted to TV image signal.Send gamma camera and display monitor central monitoring system (display) 4 to make its outer synchronous working by the synchronizing signal that micro computer produces, produce the scope that adjustable counting frame is determined image processing.Adopt microprocessor Z80A-CPU sum counter Z80A-CTC, synchronizing signal is to use by two Z80A-CTC are in parallel, produces four timing signals, and base is row, field sync signal and the composite synchronizing signal of 64 μ s and 20ms when being combined into then.Adjustable counting frame generator also is to be produced by Z80A-CTC, and the frame left side and the top that are produced by software control are fixing, and the right and bottom are adjustable, and choosing counting frame makes visual linear good in the middle of screen.Like this, by gamma camera take the photograph picture intelligence put through looking, after half digitized processing (binary conversion treatment), carry out handling and send enumerator Z80A-CTC with counting method.After microprocessor processes,, and whole indexs of measurement result can be printed by the charactron Show End Result.

This detector computer control part adopts TP801 single board computer circuit basically, and (figure is omitted) removes irrelevant parts such as recorder interface, adopts Z80A-CPU, two memory EPROM2716 able to programme and a random memory ram 6116.Solidify in the EPROM and have working procedure.

This detector host machine part 3 is by synchronous generator, counting frame signal generator, enumerator Z80A-CTC, d type flip flop, formations such as variable connector.It has following characteristics:

1. adopt the synchronous generator technology, whole system is all worked under same synchronizing signal effect.

Under microprocessor control, by two capable timing signals outputs of 0,2 passages generation of Z80A-CTC enumerator 5, form line drive signal 12 through NAND gate and d type flip flop, amplify the back and export.By two field timing signals outputs that 0,1 passage of 2 passages of 1 passage of Z80A-CTC enumerator 5, Z80A-CTC enumerator 6 and Z80A-CTC enumerator 8 produces, form field drive signal 13 through NAND gate and d type flip flop, amplify back output.Send gamma camera 1 to make its outer synchronous working simultaneously, compound capable field drive signal 14 is sent display monitor central monitoring system (display) 4.Like this, the whole system of this detector is all worked under same synchronizing signal effect, and it is acted in agreement.

2. adopt counting frame generator technology, produce counting frame signal 18 and counting frame signal 19.

This detector operational system software produces counting frame adjustable signal.It forms circuit by the counting frame and realizes: produce two line directions by 0,1,2 passages of Z80A-

CTC enumerator

7 and 2 passages of Z80A-CTC enumerator 8, the signal when counting of two field directions is confined is formed counting frame signal 18 and 19 outputs of counting frame signal by NAND gate, d type flip flop and monostable circuit 11.

3. postpone the mensuration that picture intelligence is realized " with counting method " counting and average particle sectional area with shift register.

Micro particle image signal by gamma camera 1 production is handled and half digitized processing (binary conversion treatment) through denoising, and satisfies object cutting relative theory.Picture intelligence 15 after the processing and counting frame signal 18 with, then the partly digitized microgranule signal in the counting frame is only handled.Take turns to operate by two 5G718256 bit shift register 9,10, make picture intelligence postpone 51.2 μ s.

When function control circuit makes Q be 1, then realize using the function of counting with counting method.At this moment, the micro particle image signal that the micro particle image signal of current line and lastrow promptly postpone row with after signal 17, send 0 channel counts of Z80A-CTC enumerator 6 to be accumulated as N ₂, current line micro particle image signal 16 send 1 channel counts of Z80A-CTC enumerator 6 to be accumulated as N ₁, pass through computing formula

n＝N ₁－N ₂

Be exactly actual particle number, and can on charactron, show at once.

When function control circuit makes Q be 1, then realize the mensuration of average particle sectional area.At this moment, the umber of pulse signal 17 of counting in the frame, send 0 channel counts of Z80A-CTC enumerator 6 add up N, the umber of pulse signal 16 in the counting frame in the microgranule send 1 channel counts of Z80A-CTC enumerator 6 add up N ¹

S＝ (N1)/(N) ·S／n

Wherein S is the area of counting frame, N ¹Be pixel summation in the microgranule, N is the pixel summation of counting frame, and n is a particle number.Because the size of the area S of counting frame is a datum, particle number n also is a datum, then just can draw the average particle sectional area by calculating, and can show on charactron at once.When the medical clinic applications blood routine examination, the mensuration of hematid specific volume HCT and mean corpuscular volume MCV just can be obtained by following formula:

HCT＝ (N ¹)/(N) ×25×37＋0.7

MCV＝ (HCT)/(n) ×10 ⁹（μm） ³

Equally at once, can on charactron, show.

4. adopt switch to select displayed map picture signals technology, check and guarantee the correctness of date processing.

19 outputs of counting frame signal and the micro particle image signal of being produced by gamma camera 1 mix, can send display 4 to show, see processed micro particle image intuitively, through the micro particle image 15 after more than half word processing (binary conversion treatment) and counting frame signal 18 with after, only in the counting frame micro particle image behind half digitized (binaryzation) also can send display 4 demonstrations.These two picture intelligences are selected to show by switch, thereby can be checked directly that whether the signal in the counting frame has all been handled by half digitized (binaryzation), guarantees the correctness of date processing.

In the accompanying drawing:

Fig. 1 is an intelligent particle detector contour structures sketch map.

Fig. 2 is the intelligent particle detector general diagram.

Fig. 3 A, B, C are intelligent particle detector host machine part circuit diagram.

Wherein, 1 is gamma camera, 2 is microscope, 3 is the intelligent particle detector host machine part, 4 is display (display monitor central monitoring system), 5,6,7,8 is four enumerator Z80A-CTC, 9,10 is two 256 bit shift register 5G718,11 is monostable circuit, 12 is line drive signal, 13 is field drive signal, and 14 is compound capable field drive signal, and 15 is the picture intelligence after half digitized processing (binary conversion treatment), 16 is pulse signal in current line micro particle image signal or the counting frame, 17 be current line micro particle image signal and lastrow (postponing row) the micro particle image signal with after signal or the pulse signal in the microgranule, 18 are counting frame signal, 19 for counting the frame signal.

This detector has following advantage: owing to adopt slide counting method technology, operating environment and reagent there is not specific (special) requirements, both there be not the micropore clogging, also avoid producing artificial error, and the form that can on indicator screen, observe microgranule at any time, this also is sought after hospital clinical diagnosis and medical research, and the evidence repeatability error is lower than 5%; Postpone the row way owing to adopt, reality is only stored the picture intelligence of delegation, has greatly reduced memory capacity; When microgranule (cell) is counted, owing to adopt and the counting method technology, when average area is measured,, both guaranteed counting micro particles and the precision of quadraturing owing to adopt the data compression way, guarantee the device operate as normal again; Because circuit is reliable, handle the image time very short (a few tens of milliseconds), also be feasible therefore to the count of bacteria of moving about; Simultaneously, its function is again flexibly, and it is not only applicable to biomedical sector, but also can be applicable to other field, when being used for different field, as long as software is revised a little, its function is enlarged; Because computer adopts single board computer, microgranule (cell) is counted, quadrature, is asked multiple function synthesized such as specific volume in a small-sized instrument, both be beneficial to hospital clinical to use and medical research, reduced cost again, easy to utilize.

Claims

1, a kind of counting micro particles, area and the erythrocyte volume of microscope 2, TV camera 1, display 4 and single board computer formation, intelligent particle detector that specific volume is measured of utilizing is characterized in that the line drive signal 12, field drive signal 13 and compound synchronous generator, the EPROM2716 memory of raising driving signal 14 that produce 64us, 20ms with slide counting technology, systems soft ware constitute; Clinical diagnosis is measured RBC number, leukocyte count, platelet count, mean corpuscular volume, hematid specific volume and is controlled realization with single board computer, and the counting frame generator that can automatically adjust according to different particle parameters is arranged.

2, detector according to claim 1 is characterized in that curing has working procedure in two EPROM2716 memories.

3,, it is characterized in that the main circuit of counting micro particles and average particle sectional area mensuration is made up of " with counting method " realization circuit and average particle sectional area realization circuit according to claim 1 and 2 described detectors.

4, detector according to claim 1 is characterized in that adopting single board computer to replace microcomputer, and microgranule (cell) is counted, quadratures, asks many apparatus measures technological synthesiss such as specific volume form a small-sized detector.