CN203178284U - Triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol - Google Patents
Triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol Download PDFInfo
- Publication number
- CN203178284U CN203178284U CN 201320128922 CN201320128922U CN203178284U CN 203178284 U CN203178284 U CN 203178284U CN 201320128922 CN201320128922 CN 201320128922 CN 201320128922 U CN201320128922 U CN 201320128922U CN 203178284 U CN203178284 U CN 203178284U
- Authority
- CN
- China
- Prior art keywords
- test paper
- ractopamine
- salbutamol
- joint
- pad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol. The triple test paper card is characterized in that the triple test paper card is formed by arranging three test paper cards in parallel, and comprises three test paper cards used for detecting three medicines such as clenbuterol hydrochloride, ractopamine and salbutamol, so that the beta-stimulant clenbuterol hydrochloride, ractopamine and salbutamol can be rapidly and simultaneously detected. Each test paper card for forming the triple test paper card comprises a bottom plate, and a sample absorption pad, a conjugate release pad, a reaction film and a water absorption pad which are attached to the bottom plate and are sequentially and tightly connected with one another, wherein a partial area of the conjugate release pad is covered below the sample absorption pad. According to the sample absorption pad of the test paper card, the detection liquid can be fully absorbed, is completely contacted and fully reacted with a gold-labeled antibody coated on the conjugate release pad and is reacted on the reaction film through chromatography, the error can be effectively reduced, and the triple test paper card is easy, convenient and rapid in operation, vivid, intuitive and accurate in result display, low in cost and high in sensitivity.
Description
Technical field
The utility model relates to a kind of three joint-trial paper cards that detect Clenizole Hydrochloride, Ractopamine, salbutamol.
Background technology
(the full name receptor, activator (β-adrenoceptor agonist) of β-agonist) is beta-2-agonists again to beta-stimulants, is the phenyl ethylamine class medicine of a class chemical constitution and the similar adrenaline of physiological function and norepinephrine.Common beta-stimulants is clenbuterol, Ractopamine, salbutamol, Cimaterol, Mabuterol, fenoterol, Terbutaline etc.The beta-stimulants optionally receptor, on cell membrane is combined, and plays the function of regulating sympathetic activation, lax tracheal smooth muscle, therefore is usually used in humans and animals asthma class treatment of conditions clinically.There are some researches show the eighties in 20th century, beta-stimulants influence nutriment in animal body the flow direction and redistribute, catabolism of fat in the body is strengthened, protein is synthetic increases, and significantly improves lean meat percentage.The residual meeting of beta-stimulants accumulates in the animal edible tissues, because this compounds has Orally active, if use will be to internal organs generation toxic and side effects such as the liver of people and animal, kidneys for illegal excess.Therefore as forbid for food animal chemical substance is classified this class material in countries in the world.
At present about the residual main detection method of beta-stimulants chromatography is arranged both at home and abroad, as vapor-phase chromatography (GC), high performance liquid chromatography (HPLC), gas chromatography mass spectrometry method (GC-MS), LC-MS method (LC-MS); Immunoassay is as euzymelinked immunosorbent assay (ELISA), colloid gold immune method, time resolution immunofluorescence assay etc.Chromatographic process sensitivity, accurately, high specificity, degree of separation are good, but some sample need be done thoroughly effectively pre-treatment, is not suitable for the detection of batch samples, and the equipment and instrument costliness, be difficult for popularizing.Enzyme linked immunosorbent assay is a kind of detection method of often using at present, it has fast, sensitive, can be quantitatively, to advantages such as sample purity are less demanding, be specially adapted to the detection of batch samples, but owing to need the personnel of microplate reader and skilled operation and detection time relative longer, so be not suitable for field quick detection.It is shorter that colloidal gold immunity chromatography and euzymelinked immunosorbent assay (ELISA) are compared detection time, good stability, easy and simple to handle, need not other instrument and equipments, the result judges intuitive and reliable, is applicable to carry out on-the-spot rapid screening.
The utility model content
The purpose of this utility model be to provide a kind of highly sensitive, cost is low, simple to operate, detection time short, can detect three joint-trial paper cards of Clenizole Hydrochloride, Ractopamine, salbutamol simultaneously.
Three joint-trial paper cards of detection Clenizole Hydrochloride of the present utility model, Ractopamine, salbutamol, it is characterized in that three joint-trial paper cards are formed by three test card townhouses, comprise three test cards that detect Clenizole Hydrochloride, Ractopamine, three kinds of medicines of salbutamol.Each test card that constitutes three joint-trial paper cards comprises base plate, is attached to the absorption of sample pad, bond release pad, reaction film and the adsorptive pads that closely link to each other successively on the base plate.
It is not to cover fully under the absorption of sample pad that described bond discharges pad, but the subregion is covered under the absorption of sample pad, and 1/4th zones that the preferred combination thing discharges pad are covered under the absorption of sample pad.Can prolong testing result observing time like this, the absorption of sample pad also can fully absorb tracer liquid, with the complete full contact reaction of golden labeling antibody, chromatography reacts to reaction film again, can effectively reduce error, can also prevent from detecting that some interfering components lose activity the albumen in the golden labeling antibody in the sample, influence the combination of golden labeling antibody and coating antigen.
Have parallel nature controlling line trace and detection line trace on each test card reaction film of described formation three joint-trial paper cards, be coated with the nature controlling line trace that the sheep anti mouse antiantibody constitutes on the nature controlling line, be coated with detection line trace that Clenizole Hydrochloride hapten-carrier protein conjugate constitutes on the detection line respectively, be coated with detection line trace that Ractopamine hapten-carrier protein conjugate constitutes, be coated with the detection line trace that salbutamol hapten-carrier protein conjugate constitutes.
The base plate of each test card of three joint-trial paper cards of the present utility model is the PVC plate; Reaction film is nitrocellulose filter; The absorption of sample pad is made by nonwoven fabrics or glass wool; Adsorptive pads is thieving paper; Bond discharges pad to be made by fiber, and bond discharges pad and is coated with monoclonal antibody-collaurum bond.Coupling carrier albumen on the detection line can be used bovine serum albumin(BSA), ovalbumin, human serum albumins, hemocyanin etc., and the detection line on reaction film is parallel to each other with nature controlling line and is vertical with the mobile direction of sample liquid.
The utility model test card has following beneficial effect:
1. highly sensitive.Clenizole Hydrochloride, Ractopamine, salbutamol three joint-trial paper cards are that the basis is prepared from the monoclonal antibody of colloid gold label high-affinity, priceless strong formation between gold grain and the antibody molecule in the gold labeling antibody, the two combines by the Van der Waals force between the charges of different polarity, collaurum is very little to monoclonal antibody specificity and affinity influence, and has higher mark rate.
2. easy and simple to handle quick.Need not any other reagent when using three joint-trial paper cards, as long as will splash in the test paper hole clipping with dropper after the sample pre-treatments, drip 2 ~ 3, timing behind the application of sample, observations in 5 ~ 10min.
3. cost is low, small investment.Use the quick detection test paper card, do not need to join in addition instrument and equipment and other reagent, the on-the-spot detection settled at one go, with low cost, small investment, instant effect.
4. show testing result image, accurately directly perceived.The quick detection test paper card is to show that the red line trace is as the positive and the negative marker of test card, namely have only nature controlling line to show that redness is illustrated in that this drug concentration is equal to or higher than detectability in the detected sample liquid at nitrocellulose filter, detection line and nature controlling line show all that redness is illustrated in and do not contain this medicine in the detected sample or concentration is lower than detectability, the result judges image, directly perceived, accurate, simple and clear, is not easy to occur erroneous judgement.
5. be easy to apply on a large scale.Colloidal gold test paper card is simple to operate, and sensing range is wide, can better satisfy the needs of the detection of different field.
Description of drawings
Fig. 1 is the vertical view of three joint-trial paper cards of detection Clenizole Hydrochloride, Ractopamine, salbutamol;
Fig. 2 is the structural representation of each test card of constituting three joint-trial paper cards, wherein, and 1, the absorption of sample pad; 2, bond discharges pad; 3, reaction film; 4, adsorptive pads; 5, detection line; 6, nature controlling line; 7, base plate;
Fig. 3 a is Clenizole Hydrochloride, Ractopamine, salbutamol quick detection test paper card feminine gender, and Fig. 3 b is the Clenizole Hydrochloride positive, and Fig. 3 c is the Ractopamine positive, and Fig. 3 d is the salbutamol positive, and Fig. 3 e is invalid, and wherein T is detection line, and C is nature controlling line.
Embodiment
Referring to Fig. 1: detect three joint-trial paper cards of Clenizole Hydrochloride, Ractopamine, salbutamol, is formed by three test card townhouses, comprise three test cards of detection Clenizole Hydrochloride, Ractopamine, three kinds of medicines of salbutamol.
Referring to Fig. 2: the absorption of sample pad 1 usefulness nonwoven fabrics that constitutes each test card of three joint-trial paper cards is made, bond discharges pad 2 and is adsorbed with Clenizole Hydrochloride monoclonal antibody-colloid gold label thing respectively by what fiber was made, Ractopamine monoclonal antibody-colloid gold label thing, salbutamol monoclonal antibody-colloid gold label thing, reaction film 3 is nitrocellulose filter, adsorptive pads 4 is made for absorbent material, detection line 5 is coated with the detection line trace that Clenizole Hydrochloride hapten-carrier protein conjugate constitutes respectively, be coated with the detection line trace that Ractopamine hapten-carrier protein conjugate constitutes, be coated with the detection line trace that salbutamol hapten-carrier protein conjugate constitutes, nature controlling line 6 is coated with the sheep anti mouse antiantibody, base plate 7 is the PVC backing, adhesive reaction film in order on the PVC backing, adsorptive pads, bond discharges pad and sample pad, sample pad is covered bond discharge pad 1/4th places.
1. the preparation of monoclonal antibody-colloid gold label thing
1.1 the preparation of collaurum
With two ionized waters that boil off 1% gold chloride is diluted to 0.01%, put to stir on the magnetic force heating rod stirrer and boil, every 100ml 0.01% gold chloride adds 2.5ml 1% trisodium citrate under the stirring continuing, continue to boil, be the bright heating that stops when red to liquid, supply dehydration after being cooled to room temperature, 4 ℃ of preservations.
1.2 the preparation of monoclonal antibody-colloid gold label thing
Under magnetic agitation, transfer the pH value to 7.0 of collaurum with 0.2mol/L sal tartari, the standard that adds 20 ~ 50 μ g monoclonal antibodies by every milliliter of colloidal gold solution adds the monoclonal antibody of every kind of medicine in the colloidal gold solution, continue to stir and evenly mix 10min, adding 10% bovine serum albumin(BSA) (BSA) to final concentration is the 1%(volumn concentration), leave standstill 10min.12000rpm, 4 ℃ of centrifugal 30min abandon supernatant, and precipitation is with redissolving the damping fluid washed twice, will precipitate with the redissolution damping fluid of initial collaurum volume 1/10 resuspended, put 4 ℃ standby.
Redissolution damping fluid: contain bovine serum albumin(BSA) (BSA) 0.2% ~ 0.5%(volumn concentration), the 0.02mol/L phosphate buffer of pH7.2 sucrose 1% ~ 2%(quality percentage composition).
2. Clenizole Hydrochloride, Ractopamine, salbutamol three joint-trial paper cards detect method of operating
2.1 sample pre-treatment
2.1.1 urine specimen pre-treatment
Urine specimen must be collected in clean dried, does not contain in the plastics urine cup or glass container of any antiseptic; If can not in time inspect by ready samples, urine specimen can be preserved 24h 2 ~ 8 ℃ of refrigerations, if long preservation need place-20 ℃ freezing, must guard against multigelation.
2.1.2 feed sample pre-treatments such as premix, concentrate feed, batch
(1) the feed sample that takes by weighing 2.0 ± 0.05g grinding adds the 2ml acetonitrile to 10ml polystyrene centrifuge tube, and whirling motion 1min leaves standstill 5min.
(2) it is not obvious to leave standstill the back layering as the feed sample, but the above centrifugal 5min of room temperature (20 ~ 25 ℃) 3000r/min; Or equal proportion strengthens the amount (as: take by weighing the feed sample of 5.0 ± 0.05g grinding to 50ml polystyrene centrifuge tube, add the 5ml acetonitrile) of feed sample and acetonitrile.
(3) pipette mixing in supernatant 50 μ l to the 150 μ l sample dilutions, to be checked.
2.1.3 serum sample pre-treatment
(1) serum sample must be collected in clean dried, does not contain in the plastic tube or glass container of any antiseptic; If can not in time inspect by ready samples, serum sample can be preserved 24h 2 ~ 8 ℃ of refrigerations, if long preservation need place-20 ℃ freezing, must guard against multigelation.
(2) serum sample of Shou Jiing carries out the blood coagulation processing earlier, seriously then belongs to defective serum sample as serum sample haemolysis, answers resampling; If any a small amount of haemolysis or muddiness, need the above centrifugal 5min of room temperature 3000r/min to collect supernatant, to be checked.
2.2 detect with three joint-trial paper cards
Draw sample solution to be checked with suction pipe and be added dropwise in the test card well, drip 2 ~ 3, add the back timing, reaction 5 ~ 10min observations.
2.3 testing result analysis
Clenizole Hydrochloride, Ractopamine, the salbutamol content in sample is greater than or equal to test card respectively its lowest detection is prescribed a time limit, Clenizole Hydrochloride monoclonal antibody-colloid gold label thing, Ractopamine monoclonal antibody-colloid gold label thing, salbutamol monoclonal antibody-colloid gold label thing are combined with Clenizole Hydrochloride, Ractopamine, salbutamol respectively, antigen binding site on the gold labeling antibody is closed, thereby in detection zone, because competitive reaction, can not be combined with conjugate and red stripes do not occur.Negative sample owing to lack the antigen-antibody competitive reaction, will red stripes occur at detection line and nature controlling line in testing process.
Clenizole Hydrochloride, Ractopamine, salbutamol quick detection test paper card feminine gender: when nature controlling line shows a red stripes, detection line also demonstrates a red stripes simultaneously, and the detection line color approaches or when being shallower than nature controlling line, be judged to feminine gender, shown in Fig. 3 a.
The Clenizole Hydrochloride positive: nature controlling line shows a red stripes in the card that detects clenbuterol, and detection line does not develop the color, and is judged to the positive, shown in Fig. 3 b.
The Ractopamine positive: nature controlling line shows a red stripes in the card that detects Ractopamine, and detection line does not develop the color, and is judged to the positive, shown in Fig. 3 c.
The salbutamol positive: nature controlling line shows a red stripes in the card that detects salbutamol, and detection line does not develop the color, and is judged to the positive, shown in Fig. 3 d.
Invalid: when nature controlling line does not demonstrate red stripes, then no matter whether detection line demonstrates red stripes, and it is invalid that this test card is judged to, shown in Fig. 3 e.
Claims (5)
1. detect three joint-trial paper cards of Clenizole Hydrochloride, Ractopamine, salbutamol, it is characterized in that three joint-trial paper cards are formed by three test card townhouses, comprise three test cards that detect Clenizole Hydrochloride, Ractopamine, three kinds of medicines of salbutamol.
2. three joint-trial paper cards as claimed in claim 1, it is characterized in that each test card that constitutes three joint-trial paper cards comprises base plate, is attached to the absorption of sample pad, bond release pad, reaction film and the adsorptive pads that closely link to each other successively on the base plate, the subregion that bond discharges pad is covered under the absorption of sample pad.
3. three joint-trial paper cards as claimed in claim 1, it is characterized in that having parallel nature controlling line trace and detection line trace on each test card reaction films of described formation three joint-trial paper cards, be coated with the nature controlling line trace that the sheep anti mouse antiantibody constitutes on the nature controlling line, be coated with detection line trace that Clenizole Hydrochloride hapten-carrier protein conjugate constitutes on the detection line respectively, be coated with detection line trace that Ractopamine hapten-carrier protein conjugate constitutes, be coated with the detection line trace that salbutamol hapten-carrier protein conjugate constitutes.
4. three joint-trial paper cards as claimed in claim 2 is characterized in that, 1/4th zones that the preferred combination thing discharges pad are covered under the absorption of sample pad.
5. as claim 2 or 4 described three joint-trial paper cards, it is characterized in that described bond discharges pad and is coated with monoclonal antibody-collaurum bond.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320128922 CN203178284U (en) | 2013-03-20 | 2013-03-20 | Triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320128922 CN203178284U (en) | 2013-03-20 | 2013-03-20 | Triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203178284U true CN203178284U (en) | 2013-09-04 |
Family
ID=49074963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320128922 Expired - Fee Related CN203178284U (en) | 2013-03-20 | 2013-03-20 | Triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203178284U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107101991A (en) * | 2017-05-31 | 2017-08-29 | 东南大学 | A kind of high sensitivity multiplex detection chromatograph test strip |
| CN111551711A (en) * | 2020-06-11 | 2020-08-18 | 山西师范大学 | Detection test paper for food beta-agonist, preparation method and multi-connected detection card |
| CN116626284A (en) * | 2023-07-24 | 2023-08-22 | 南京启迈生物科技有限公司 | Double-sided window single-sample-adding multi-joint anti-pollution rapid detection test paper card and preparation method thereof |
-
2013
- 2013-03-20 CN CN 201320128922 patent/CN203178284U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107101991A (en) * | 2017-05-31 | 2017-08-29 | 东南大学 | A kind of high sensitivity multiplex detection chromatograph test strip |
| CN111551711A (en) * | 2020-06-11 | 2020-08-18 | 山西师范大学 | Detection test paper for food beta-agonist, preparation method and multi-connected detection card |
| CN116626284A (en) * | 2023-07-24 | 2023-08-22 | 南京启迈生物科技有限公司 | Double-sided window single-sample-adding multi-joint anti-pollution rapid detection test paper card and preparation method thereof |
| CN116626284B (en) * | 2023-07-24 | 2023-10-20 | 南京启迈生物科技有限公司 | Double-sided window single-sample-adding multi-joint anti-pollution rapid detection test paper card and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100482787C (en) | Immune colloid gold test paper strip for detecting sulfadiazine residue and its preparation method | |
| CN104569399B (en) | A kind of test strips detecting ochratoxin A and application thereof | |
| CN101852800A (en) | Colloidal gold test strip for semi-quantitatively detecting concentration of tacrolimus drug in human whole blood and detection method | |
| CN201181297Y (en) | Colloidal gold test paper card for detecting Ractopamine medicine residue | |
| CN102288753A (en) | Quadruple colloidal gold immunochromatography testing strip for rapid detection of residual tetracycline, chlortetracycline, oxytetracycline and doxycycline and preparation method of same | |
| CN102565382B (en) | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples | |
| CN105842464B (en) | Joint based on up-converting phosphor technology quantitatively detects uNGAL and uCr device and preparation method thereof | |
| CN203178284U (en) | Triple test paper card for detecting clenbuterol hydrochloride, ractopamine and salbutamol | |
| CN106248894A (en) | A kind of paper chip detecting Residue of Antibiotics in Milk | |
| CN102135498A (en) | Semi-quantitative colloidal metal detection technology taking multi-capture property as characteristic and preparation method and use thereof | |
| CN102135535A (en) | Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application | |
| CN103575887A (en) | Test paper card for detecting aflatoxin B1 and application of test paper card | |
| CN102213723A (en) | Doxycycline detection kit and preparation method thereof | |
| CN102297970A (en) | Two-joint detection test strip for rapidly detecting residue of cyromazine (CA) and melamine (MA) and preparation method thereof | |
| CN107271665A (en) | A kind of test strips for detecting salbutamol and its application | |
| CN102539412A (en) | Magnetic particle chemical luminous kit for detecting clenbuterol and application thereof | |
| CN102608316A (en) | Kit or test strip for detecting quinoxaline compound | |
| CN107290520A (en) | Determine the immuno-chromatographic test paper strip of the anti-A of pregnant woman IgG and anti-blood group antibody B or human blood type reverse type | |
| CN202903794U (en) | Colloidal gold test card for detecting aflatoxin B1 residues | |
| CN103513035A (en) | Test strip and method for detecting aflatoxin M1 | |
| CN103018451A (en) | Enzyme-linked immunoassay kit for chloramphenicol detection, and applications thereof | |
| CN103364546B (en) | A kind of kit and method detecting Furaxone metabolite | |
| CN102680621B (en) | A kind of chemiluminescence detection technology and application thereof | |
| CN201876457U (en) | Colloidal gold test paper card for testing of sulfanilamide drug residue | |
| CN101980021A (en) | Colloidal gold chromatography test strip for quickly detecting sibutramine and derivative thereof and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130904 Termination date: 20210320 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |