CN203101392U - Detection kit for detecting encephalitis B through collecting body fluid by using swab - Google Patents
Detection kit for detecting encephalitis B through collecting body fluid by using swab Download PDFInfo
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- CN203101392U CN203101392U CN 201220596711 CN201220596711U CN203101392U CN 203101392 U CN203101392 U CN 203101392U CN 201220596711 CN201220596711 CN 201220596711 CN 201220596711 U CN201220596711 U CN 201220596711U CN 203101392 U CN203101392 U CN 203101392U
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Abstract
The utility model relates to the technical field of biological applications and particularly relates to a detection kit for detecting encephalitis B through collecting body fluid by using a swab. According to the detection kit, the swab is adopted to collect oral fluid, and colloidal gold is used for labeling an E-gene recombinant antigen of encephalitis B virus and coating an anti-human-IgG (Immunoglobulin G) antibody, so that the detection on encephalitis B virus IgG resisting antibodies in the oral fluid is realized. The detection kit has the advantages of being simple and convenient in operation, fast in response, high in sensitivity, strong in specificity, applicable to on-site detection, economical and practical and the like.
Description
Technical field
The utility model relates to the biologic applications technical field, particularly relates to a kind of detection kit of using the swab collection of body fluids to detect encephalitis B.
Background technology
Encephalitis B virus causes Japanese Type-B encephalitis, is a kind of acute infectious disease by killing propagation.The case fatality rate of encephalitis and disability rate height are particularly one of the main infectious diseases of children's health of threat crowd.Be onset peak season summer and autumn, and popularly district's distribution is closely related with the distribution of media mosquito, and China is the high popular district of encephalitis, once is very popular in the sixties in 20th century and the initial stage seventies whole nation, maintains lower morbidity level in recent years.National in recent years encephalitis reported cases are counted every year between 5000～10000 examples, break out or popular but have during some areas.And whole world case load every year up to 50000 examples, death toll 15000 examples.
The main method that encephalitis B is detected is that blood sample is carried out acquisition testing antibody at present, in the detection method of collaurum, the report that detects encephalitis B IgG is arranged, but use the oral fluid swab to carry out the IgG detection relevant report is not arranged as yet.Compare with conventional reagents, use the oral fluid swab to carry out the IgG detection and have sampling easily, reduce patient's misery, reduce the advantage of testing staff's workload.
Summary of the invention
The purpose of this utility model provides a kind of detection kit of using the swab collection of body fluids to detect encephalitis B, has advantages such as sampling is easy, easy and simple to handle, reaction is quick, susceptibility is high, high specificity, suitable on-the-spot detection.
The utility model provides a kind of detection kit of using the swab collection of body fluids to detect encephalitis B, it is characterized in that plastic support board (7) one ends connect upward sample pad (1), the tight crimping of one end of last sample pad (1) contains the collaurum pad (2) of underlined japanese encephalitis virus E gene recombinant antigens, the tight crimping cellulose nitrate of collaurum pad (2) one ends (NC) films (3), nitrocellulose filter is coated with detection line T line (5) and the Quality Control C line (6) that is separated from each other, detection line T (5) is for being coated on the anti-human IgG antibody on the NC film, Quality Control (C) line (6) is for being coated on the antibody of the anti-japanese encephalitis virus E gene recombinant antigens on the NC film, and the other end of nitrocellulose filter connects inhales sample pad (4) formation test paper.
Described a kind of detection kit of using the swab collection of body fluids to detect encephalitis B, the method for coating that it is characterized in that its antibody is: will resist the human IgG antibody to be mixed with the solution of 5mg/ml with 0.01M pH7.2 phosphate buffer (PBS), rule with the parameter of 1-1.5ul/cm in NC film bottom with spray film instrument, bag is by the T line, wrap by the antibody of anti-japanese encephalitis virus E gene recombinant antigens on NC film top simultaneously as the C line, after the line with the NC film at drying room, temperature 20-25 ℃, humidity is less than 30%, dry 2-5 hour.
The colloid gold particle of the E gene recombinant antigens of the japanese encephalitis virus that mark is good is made the collaurum pad, is prepared into test strips with the nitrocellulose filter of coated antibody and other auxiliary materials.
Described a kind of detection kit of using the swab collection of body fluids to detect encephalitis B is characterized in that its detection method is:, slowly press from a left side and to wipe away the right side from one jiao of the last gum of mouth with buccal swab nylon cushion end, 5 to 6 seconds times spent, then, slowly press from the right side and to wipe away a left side, 5 to 6 seconds times spent, 3 times repeatedly, the buccal swab of reversing then is from one jiao of the lower gum of mouth, repeat said process, pressure is wiped away dynamics, with the person under inspection acid sense of expanding is arranged, and does not have significant discomfort and be as the criterion; After pressure is wiped away, immediately buccal swab is immersed in the sample buffer, test tube wall is close on the two sides of elder generation's relief swab, swipe 6-8 time up and down, liquid in pipe is the oral fluid sample, and test strips is kept flat, on last sample pad, add the 100ul sample, make collaurum dissolving and on the NC film chromatography, the appearance situation of Direct observation C, T line in 15 minutes with the naked eye then, and judge testing result.
The beneficial effects of the utility model are: a kind of detection kit of using the swab collection of body fluids to detect encephalitis B is provided.Compare with conventional reagents, use the oral fluid swab to carry out the IgG detection and have sampling easily, reduce patient's misery, reduce the advantage of testing staff's workload.
Description of drawings:
Fig. 1 uses the swab collection of body fluids to detect the detection kit test paper structural representation of encephalitis B.
Reference numeral explanation: 1: go up the sample pad; 2: the collaurum pad; The 3:NC film; 4: inhale the sample pad
5: detection line T1; 6: Quality Control (C) line; 7: plastic support board.
Fig. 2 gathers oral fluid swab structural representation
Reference numeral explanation: 1: the nylon cushion end; 2: handheld terminal.
Embodiment
Embodiment:
The detection kit test paper of preparation encephalitis B
Japanese encephalitis virus E gene recombinant antigens is used colloid gold label, make the collaurum pad, to resist the human IgG antibody to be mixed with the solution of 5mg/ml with 0.01M pH7.2 phosphate buffer (PBS), rule with the parameter of 1-1.5ul/cm in NC film bottom with spray film instrument, bag is wrapped by the antibody of japanese encephalitis virus E gene recombinant antigens on NC film top as the C line simultaneously by the T line, after the line with the NC film at drying room, temperature 20-25 ℃, humidity is less than 30%, dry 2-5 hour.Collaurum pad and bag are assembled into test strips by good NC film and other auxiliary materials.
As person to be checked, the healthy people who 50 examples has been made a definite diagnosis encephalitis B IgG negative antibody organizes person to be checked in contrast with the patient that makes a definite diagnosis encephalitis B IgG antibody positive., slowly press from a left side and to wipe away the right side, 5 to 6 seconds times spent from one jiao of the last gum of mouth with buccal swab nylon cushion end, then, slowly press from the right side and to wipe away a left side, 5 to 6 seconds times spent, 3 times repeatedly, the buccal swab of reversing then, from one jiao of the lower gum of mouth, repeat said process, press the dynamics of wiping away, with the person under inspection acid sense of expanding is arranged, and do not have significant discomfort and be as the criterion; After pressure is wiped away, immediately buccal swab is immersed in the sample buffer, test tube wall is close on the two sides of elder generation's relief swab, swipe 6-8 time up and down, liquid in pipe is the oral fluid sample, and test strips is kept flat, on last sample pad, add the 100ul sample, make collaurum dissolving and on the NC film chromatography, the appearance situation of Direct observation C, T line in 15 minutes with the naked eye then, and judge testing result.
The explanation of testing result:
If the control line in the check plot does not occur, testing result is invalid, if a line control line only occurs in the check plot, the result is negative.If detection line and control line all occur, two lines promptly appear on the test paper, and then testing result is positive.
Detect by clinical serum, the sample that sample that IgG is positive and IgG are negative, detection of this reagent and clinical diagnosis result are in full accord.Clinical detection shows that the performance of this reagent compares there was no significant difference with existing diagnostic means, is suitable for clinical detection.
Claims (1)
1. detection kit of using the swab collection of body fluids to detect encephalitis B, it is characterized in that plastic support board (7) one ends connect upward sample pad (1), the tight crimping of one end of last sample pad (1) contains the collaurum pad (2) of underlined japanese encephalitis virus E gene recombinant antigens, the tight crimping nitrocellulose filters of collaurum pad (2) one ends (3), nitrocellulose filter is coated with detection line T line (5) and the Quality Control C line (6) that is separated from each other, detection line T (5) is for being coated on the anti-human IgG antibody on the nitrocellulose filter, Quality Control (C) line (6) is for being coated on the antibody of the encephalitis B E gene recombinant antigens on the nitrocellulose filter, and the other end of nitrocellulose filter connects inhales sample pad (4) formation test paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220596711 CN203101392U (en) | 2012-11-12 | 2012-11-12 | Detection kit for detecting encephalitis B through collecting body fluid by using swab |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220596711 CN203101392U (en) | 2012-11-12 | 2012-11-12 | Detection kit for detecting encephalitis B through collecting body fluid by using swab |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203101392U true CN203101392U (en) | 2013-07-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220596711 Expired - Fee Related CN203101392U (en) | 2012-11-12 | 2012-11-12 | Detection kit for detecting encephalitis B through collecting body fluid by using swab |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203101392U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242048A (en) * | 2014-07-11 | 2016-01-13 | 郑兆珉 | Detection kit and detection method |
-
2012
- 2012-11-12 CN CN 201220596711 patent/CN203101392U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242048A (en) * | 2014-07-11 | 2016-01-13 | 郑兆珉 | Detection kit and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130731 Termination date: 20141112 |
|
| EXPY | Termination of patent right or utility model |