CN203079952U - Unicellular gesture adjusting device based on swirling flow - Google Patents
Unicellular gesture adjusting device based on swirling flow Download PDFInfo
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- CN203079952U CN203079952U CN 201320001347 CN201320001347U CN203079952U CN 203079952 U CN203079952 U CN 203079952U CN 201320001347 CN201320001347 CN 201320001347 CN 201320001347 U CN201320001347 U CN 201320001347U CN 203079952 U CN203079952 U CN 203079952U
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Abstract
The utility model provides a unicellular gesture adjusting device based on swirling flow. The unicellular gesture adjusting device comprises two micropipes for jetting fluid, wherein one end of each of the two micropipes is connected with a micropump through a hose, and the other end of each of the two micropipes is used for jetting unicells; and each hose is provided with a device for extruding and releasing the hose to realize pulse jet. According to the unicellular gesture adjusting device, the end surface distance and the axial distance of the two parallel micropipes are adjusted according to shapes and sizes of the cells in a cell gesture adjustment process; the two micropipes are controlled to simultaneously jet fluid to generate swirling flow; and the cells are driven to rotate by using the viscosity force of the swirling flow, and any angle of the cells can be adjusted by a pulse jet manner. The unicellular gesture adjusting device can be applicable to changes of shapes and sizes of different cells and the solution viscosity, the cells are accurately rotated to reach a desirable gesture without generating mechanical damages to the cells, and the success rate of cell experiment operation is favorably improved.
Description
Technical field
The utility model relates to unicellular attitude regulation technology field, is specifically related to the unicellular attitude regulating mechanism based on swirling flow.
Background technology
It is difficult point problem in the biotechnology that the cell attitude is regulated.At present, it is to adopt the micropin pipe to fiddle with cell to make its rotation that the cell attitude of practical application is regulated working method, exist problems such as the pair cell damage is big, precision is low, repeatability is low, cost is high, labour intensity is big, restricted popularizing and using of micromanipulative technique greatly.The scientific research personnel generally wishes to replace with machinery manual, and is manual with automatic replacement, makes that micromanipulative technique can be oversimplified, automatization, and then realizes through engineering approaches.
The cell attitude control method of direct contact type can hurt cell, the success ratio of influence experiment, and also the efficient of cell manipulation is also very low.The injury that brings to cell can be avoided or reduce to contactless control method, is the main flow of domestic and international researchist's research.Contactless cell attitude control method mainly contains laser method, dielectrophoresis method, electrostatic field method, supersonic method, microbot vibratory drilling method, two microtubule convection current methods etc. at present both at home and abroad.The damage of pair cells such as laser method, dielectrophoresis method, electrostatic field method, supersonic method is also failed clearly, and the needed technical equipment of these methods is all very expensive, is unfavorable for realizing engineering industryization.Microbot vibratory drilling method, two microtubule convection current methods all are to belong to the method pair cell that adopts microfluid to carry out the attitude adjusting.The microbot vibratory drilling method is to produce certain flow field around making it by the vibration microbot, comprises swirling flow field.These flow fields can be guided to cell at a distance near the bar, also cell can be rotated.The frequency and the amplitude of the vibration of glass microbot in this method, need adjust according to the variation of cell parameters, particularly when cell size and soltion viscosity variation, need demarcate and be provided with vibrational frequency and amplitude again, even redesign, will cause the difficulty of practical application.The two microtubule convection current methods that the Zhang Weiyi of Institutes Of Technology Of Nanjing proposes are that cell is transported in two horizontal planes between the disalignment jet pipe by the storage conduit, and the flow field that produces during the spouting of liquid convection current is rotated cell in horizontal plane.Then be to adopt the injection fluid pushing away cell rolling in perpendicular to reach the orientation that needs.But because two spray tubes are to be fixed in the horizontal plane, then the requirement of pair cell size is a fixed, and the positioning action before the cell attitude is adjusted is relatively more difficult.As seen, in existing cell attitude control method based on microfluidic, generally can only be at certain fixed measure, the cell of shape and the nutrient solution of certain viscosity, when these parameters changed, the driving of microfluid and controlled variable all must be readjusted, even needed redesign.
Under such background, the utility model proposes unicellular attitude control method based on swirling flow.This method can be according to the variation of cell size, shape, nutrient solution viscosity change etc., by the relative distance of adjusting microtubule and size and the intensity that the microtubule jet velocity is freely regulated swirling flow, carry out pulse jet by extruding with the silica gel hose that release is connected microtubule, realize the accurate adjusting of cell attitude.
The utility model content
The utility model purpose is to overcome the art methods above shortcomings, and a kind of unicellular attitude regulating mechanism based on swirling flow is provided, and can realize driving the angle that cell rotates to hope contactlessly, and concrete technical scheme is as follows.
Based on the unicellular attitude regulating mechanism of swirling flow, it comprises that two are used to spray the fluidic microtubule, and these two microtubules all have an end to be connected with Micropump by flexible pipe, and the other end all is used for to unicellular injection fluid; Described flexible pipe is provided with and is used to push and discharges flexible pipe and realize the device of pulse jet.
Further optimize, described two microtubule shapes are identical with size.
Further optimize, described two microtubules are parallel to each other.
Further optimize, describedly be used to push and discharge flexible pipe and realize that the device of pulse jet comprises piezoelectric ceramics.
Further optimize, the distance of the fluid outlet of two microtubules is 0.5:1 ~ 5:1 with the ratio of cell dia.
Further optimize, the distance of the fluid outlet of two microtubules is 1:1 ~ 3:1 with the ratio of cell dia.
Further optimize, the fluid outlet of the axle base of two microtubules and two microtubules is apart from consistent.
Utilize the unicellular attitude control method based on swirling flow of said apparatus: in the cell attitude is regulated operating process, the end face distance and the axle base of the identical microtubule that is parallel to each other according to two of the shape size adjustment of cell; Control two microtubules and spray fluid generation swirling flow simultaneously; Utilize the viscous force of swirling flow to drive the cell rotation, and the mode by pulse jet realize that cell regulates at any angle.Described pulse jet is to push and discharge the silica gel hose that is connected Micropump and microtubule and finish by piezoelectric ceramics.Adopt the mode of described pulse jet to drive angle ground rotation of angle of cell to reach required attitude.
Aforesaid method is further optimized, described pulse jet is realized by the control flow rate of fluid, described pulse jet is realized by the control flow rate of fluid, the low flow velocity of the fluid of pulse jet is 0.01 ~ 0.1m/s, the fluid high flow rate of pulse jet is 0.05 ~ 1.0m/s, and high flow rate makes jet velocity and time relation be pulse condition greater than low flow velocity., the fluid high flow rate of pulse jet is 0.1 ~ 0.5m/s.Described pulse jet is meant the nozzle-fluid velocity that increases suddenly microtubule before cell fails to rotate soon, is reduced to original lower velocity level immediately rapidly, makes the jet velocity of microtubule and the shape that time relation is pulse.
Compared with prior art, the utlity model has significant advantage:
(1) can freely adjust relative position between the microtubule with the needs that the geomery that adapts to different cells and soltion viscosity change, improve the versatility of cell attitude control method.(2) utilize swirling flow to drive the adjusting that the cell rotation realizes its attitude, can pair cell do not produce any mechanical wounding (3) and adopt the mode of pulse jet can drive the angle that cell accurately rotates to hope, promptly realize the accurate adjusting of cell attitude.
Description of drawings
Fig. 1 is the relative dimensions graph of a relation of two microtubules and cell.
Fig. 2 is the interface chart of microtubule and piezoelectric ceramic device and Micropump.
Fig. 3 is the pulse jet velocity peak values that obtains according to experiment and the graph of relation of cell angle of rotation.
Fig. 4 is for pushing in the example and discharging the synoptic diagram that the silica gel hose that is connected microtubule carries out pulse jet.
Embodiment
Below in conjunction with accompanying drawing concrete enforcement of the present utility model is described further, but enforcement of the present utility model and protection domain are not limited thereto.
Unicellular attitude control method based on swirling flow comprises that mainly following step realizes the attitude adjusting of cell:
(1) as Fig. 1, based on the unicellular attitude regulating mechanism of swirling flow, it comprises that two are used to spray fluidic microtubule (1 and 2), and these two microtubules all have an end to be connected with Micropump 6 by flexible pipe, and all have an end to be used to spray unicellular; Described flexible pipe is provided with and is used to push and discharges flexible pipe and realize the device (this example adopts piezoelectric ceramics) of pulse jet.According to the geomery of cell 3, make cell be in first and move the symmetry centre position that microtubule 1 and second moves microtubule 2, and the ratio of regulating the diameter b of the end face distance a of two microtubules and cell 3 is: 1:1 ~ 3:1.
(2) keep the end face distance a of two microtubules (1 and 2) constant, till regulating between the internal diameter of two microtubules (1 and 2) distance b and its end face distance a being consistent, even a=b; In the regulate process of step (1) and step (2), cell 3 is in the symmetry centre position of two microtubules (1 and 2) all the time.
(3) see Fig. 2, opening Micropump 6 is that two microtubules (1 and 2) provide flow, but the less cell that makes of the jet velocity of control microtubule fails to rotate; Be useful on extruding and discharge flexible pipe and realize device 5(such as piezoelectric ceramic device, the step motor etc. of pulse jet) feed electric current, it is discharged behind extruding silica gel hose 4 suddenly immediately; The extruding silicone tube increases tube fluid (as enchylema or water) flow velocity, after the pulsating pressure effect, speed returns to original low speed level, pulse operating time 0.1-0.5, preferred 0.2s, cell 3 will stop behind the Small angle of rotation (15-150 °) in the swirling flow that speed changes suddenly.As an example, can make low speed microtubule ejecting fluid speed herein is 0.04m/s, and ejecting fluid speed is 0.45m/s when pushing silica gel hose suddenly.
(4) continue to drive angle ground rotation of 3 one angles of cell with pulsation method identical in the step (3), until reaching the attitude that needs.
In the above-mentioned unicellular attitude control method based on swirling flow, to regulate the end face distance a of two microtubules (1 and 2) and the distance b between the microtubule diameter according to the geomery of cell 3, be in order to make cell 3 can in swirling flow, press the dead axle rotation, promptly can in the attitude regulate process, not blown away.
The pulse mode of above-mentioned employing drives in the method for cell rotation, and the angle of cell 3 each step rotation is relevant with the velocity peak values and the injecting time of pulse jet, and velocity peak values is big more, and injecting time is long more, and the angle of cell 3 rotations is just big more.The velocity peak values of concrete pulse jet and injecting time need be converted into the pulsed current that piezoelectric ceramic device needs then through demarcating.Experiment shows that under the identical situation of injecting time, the jet velocity peak value becomes good linear relationship with the cell angle of rotation, as shown in Figure 3.A kind of extruding of using in the experiment and discharge the device of the silica gel hose that is connected microtubule, the device that carries out pulse jet as shown in Figure 4,401 is stepper-motor, 402 are the mobile terminal of pulse squeezing device, 403 are the inboardend of pulse squeezing device.Stepper-motor can be realized the mobile accuracy of 0.5um, thereby and can control step speed change the pulsating pressure size.
Claims (7)
1. based on the unicellular attitude regulating mechanism of swirling flow, it is characterized in that comprising that two are used to spray the fluidic microtubule, these two microtubules all have an end to be connected with Micropump by flexible pipe, and the other end all is used for to unicellular injection fluid; Described flexible pipe is provided with and is used to push and discharges flexible pipe and realize the device of pulse jet.
2. the unicellular attitude regulating mechanism based on swirling flow according to claim 1 is characterized in that described two microtubule shapes are identical with size.
3. the unicellular attitude regulating mechanism based on swirling flow according to claim 2 is characterized in that described two microtubules are parallel to each other.
4. according to right 1 described unicellular attitude regulating mechanism, it is characterized in that describedly being used to push and discharging flexible pipe and realize that the device of pulse jet comprises piezoelectric ceramics based on swirling flow.
5. according to right 1 described unicellular attitude regulating mechanism, it is characterized in that the ratio of distance and cell dia of the fluid outlet of two microtubules is 0.5:1 ~ 5:1 based on swirling flow.
6. according to right 1 described unicellular attitude control method, it is characterized in that the ratio of distance and cell dia of the fluid outlet of two microtubules is 1:1 ~ 3:1 based on swirling flow.
7. according to right 1 described unicellular attitude control method based on swirling flow, the axle base that it is characterized in that two microtubules and the fluid outlet of two microtubules are apart from consistent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320001347 CN203079952U (en) | 2013-01-04 | 2013-01-04 | Unicellular gesture adjusting device based on swirling flow |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320001347 CN203079952U (en) | 2013-01-04 | 2013-01-04 | Unicellular gesture adjusting device based on swirling flow |
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| Publication Number | Publication Date |
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| CN203079952U true CN203079952U (en) | 2013-07-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320001347 Expired - Fee Related CN203079952U (en) | 2013-01-04 | 2013-01-04 | Unicellular gesture adjusting device based on swirling flow |
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2013
- 2013-01-04 CN CN 201320001347 patent/CN203079952U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130724 Termination date: 20160104 |