CN202956532U - Laser coherence diffraction micro-imaging device - Google Patents
Laser coherence diffraction micro-imaging device Download PDFInfo
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- CN202956532U CN202956532U CN 201220645458 CN201220645458U CN202956532U CN 202956532 U CN202956532 U CN 202956532U CN 201220645458 CN201220645458 CN 201220645458 CN 201220645458 U CN201220645458 U CN 201220645458U CN 202956532 U CN202956532 U CN 202956532U
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Abstract
The utility model discloses a laser coherence diffraction micro-imaging device. The device comprises a laser, an attenuation sheet, a first lens, a first diaphragm, a second lens, a second diaphragm, a sample table, a baffle and a charged coupled device (CCD) image sensor which are arranged coaxially along a beam forwarding direction successively, a stepping frame and a computer. The stepping frame can move the CCD image sensor left and right, up and down and front and back, and the computer is connected with the CCD image sensor. According to the laser coherence diffraction micro-imaging device, the lens groups and the attenuation sheet are used for compressing light beams, so that the luminous flux is improved, and the resolution ratio can be improved to submicron; the CCD image sensor moves to acquire high-angle and low-angle diffraction signals respectively, so that a single diffraction pattern can be composited; and three-dimension imaging can be achieved through a sample rotation method, and dynamic real-time imaging can be performed.
Description
Technical field
The utility model relates to a kind of laser coherence diffraction microscopic imaging device.
Background technology
Coherent diffraction imaging (coherence diffraction imaging, CDI) technology is a kind of new imaging technique grown up the nearest more than ten years, it is noncrystal that it makes the method for optical diffraction amalyzing substances structure expand to from crystal, at subjects such as physics, chemistry, biology, materials, important application prospect arranged.
The ultimate principle of coherent diffraction is that plane light wave is after the object diffraction, the wavefront in far field is the Fourier transform from object outgoing light wave, detector can record light intensity, but can't obtain the phase information of light wave, but can be by excessively sampling, iterative algorithm recovers amplitude and the phase place of light wave, thereby rebuilds the image of object.It has three important application directions: the first, the mensuration of non-crystalline material three-dimensional structure, comprise defect and stress field in nanocrystal determine and non-Ordered Materials as the quantitative three-dimensional imaging of nano particle and biomaterial; The second, the three-dimensional imaging of whole cell, the location of mainly being devoted to special polyprotein matter complex in cell; The 3rd, adopt the Potential feasibility of extremely strong ultrashort X ray pulse to the imaging of single larger protein complex.
Current research shows, by the single width diffraction pattern, also can obtain the three-dimensional structure of object.For the diffraction pattern of a limited object, when enough sampling in large scope on the Ai Waer ball, the image of the three-dimensional of object can be determined by two-dimentional diffraction pattern.This method can be determined the three-dimensional structure of sample, and does not need sample is rotated, scans and cuts into slices.
The coherent diffraction experiment generally completes on high-quality synchrotron radiation light source, application third generation synchrotron radiation light source, the resolution of its imaging can reach several nanometers, the absorption imaging of synchrotron radiation light source and coaxial phase contrast experiment resolution are high a lot of relatively, along with the 4th generation the light source X-ray free-electron laser development, coherent diffraction imaging will be more widely used.
The visible report of the coherent diffraction imaging of laser is few, the method that has all adopted lens to be expanded in these reports, sample size is several millimeters, resolution is at tens microns, the size of light beam and luminous flux have limited the size of sample and the resolution of image, can not carry out to less samples such as cells the real time imagery of 3 D stereo.
Summary of the invention
Order of the present utility model is to propose a kind of scioptics and attenuator compression light beam, realizes the laser coherence diffraction microscopic imaging device of micro-meter scale sample.
Laser coherence diffraction microscopic imaging device described in the utility model, it is characterized in that: described device is shown laser instrument, attenuator, first lens, the first diaphragm, the second lens, the second diaphragm, sample stage and is fixed its runing rest, baffle plate and ccd image sensor along light beam working direction coaxial row successively, and can realize about ccd image sensor, upper and lower, the stepping frame that moves forward and backward, with the computing machine that is connected ccd image sensor; Wherein said attenuator, first lens, the first diaphragm, the second lens, the second diaphragm are fixed on optical bench, described baffle plate is fixed on ccd image sensor, the ccd image sensor fixed placement two orthogonal can left and right, on the stepping frame that moves up and down, and then be fixed on the stepping frame that can move forward and backward along optical path direction.
Further, in above-mentioned laser coherence diffraction microscopic imaging device: described attenuator attenuation multiple is preferably 10 times～1000 times; The focal length of described first lens and the second lens is preferably respectively 50mm～3000mm, the focus of two lens overlaps, two lens are in the ratio compression light beam of focal length, and the first diaphragm and the second diaphragm are placed on respectively on the focus of first lens and the second lens for eliminating the parasitic light of light path; Described sample stage is placed on the waist spot place of laser, and the runing rest rotation angle range of sample stage is 0 degree～180 degree; Described baffle plate is positive square, and the preferred 1mm～3mm of the length of side is arranged on the path of laser direct projection ccd image sensor for blocking direct projection to the laser on CCD; The diffracted signal that described ccd image sensor is collected is transferred to computing machine.
The application of laser coherence diffraction microscopic imaging device described in the utility model:
Dispose a set of laser coherence diffraction imaging device, described device is shown laser instrument 1, attenuator 2, first lens 3, the first diaphragm 4, the second lens 5, the second diaphragm 6, sample stage 7 and is fixed its runing rest, baffle plate 8 and ccd image sensor 9 along light beam working direction coaxial row successively, and can realize about ccd image sensor, upper and lower, the stepping frame that moves forward and backward, with the computing machine 10 that is connected ccd image sensor; 10 times～1000 times of the attenuation multiples of selection attenuator; Select the focal length 50mm～3000mm of two lens, two lens are in the ratio compression light beam of focal length; Sample is fixed on sample stage; Select the centre position of diffracted signal, use the imageing sensor collection signal; Then adjust two orthogonal stepping framves, mobile ccd image sensor, to the upper left corner, the lower left corner, the upper right corner and the lower right corner of diffracted signal, gathers respectively the high angle signal of four positions; Then adjust the stepping frame of placing along light path, ccd image sensor is moved away from sample along light path, according to identical time shutter and exposure frequency, collect more low-angle diffracted signal; Diffracted signal all is saved in computing machine; Ccd image sensor is synthesized in computing machine to individual diffraction image at the diffracted signal of collecting, with iterative algorithm, just can rebuild object amplitude and phase place.Then rotary sample, every rotation once, just repeats the step of above-mentioned signals collecting, amplitude and Phase Build Out, obtains one group of amplitude and the phase image of sample; 3-D view by one group of amplitude of sample and phase image with the method synthetic body of tomography.
Laser coherence diffraction imaging device described in the utility model utilizes lens and attenuator compression light beam, effectively dwindled light beam and improved luminous flux, and then improved the resolution of imaging, can realize the diffracted signal collection of the sample of micro-meter scale, the ccd image sensor of collection signal can be along being moved on light path and the direction perpendicular to light path, gather respectively high angle and low-angle diffracted signal, these signals synthesize the single width diffraction pattern, the scope of fourier space and the contrast of diffraction pattern have been improved, resolution can be brought up to sub-micron, and can carry out dynamic real time imagery.The utility model not needing sample is contacted, cut into slices, obtain the 3 D stereo realtime graphic under the condition of dyeing and fluorescence, there is significant application value for analyzing micron-scale sample structure, variation and forming process.
The accompanying drawing explanation
Fig. 1 is the schematic diagram of a kind of laser coherence diffraction of the utility model micro imaging method.
Wherein: laser instrument 1, attenuator 2, lens 3, diaphragm 4, lens 5, diaphragm 6, sample 7, baffle plate 8, ccd image sensor 9, computing machine 10.
Fig. 2 is a sample of coherent diffraction imaging, is the rectangle of microsphere silica gel arrangement.
Fig. 3 is the experimental result of embodiment 1.
Fig. 4 is the material picture that utilizes excessively sampling and iterative algorithm to rebuild.
Embodiment
Below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that as described below specific embodiment is only in order to explain the utility model, and be not used in restriction the utility model.
Embodiment 1:
As Fig. 1, laser coherence diffraction microscopic imaging device described in the utility model is shown laser instrument 1, attenuator 2, first lens 3, the first diaphragm 4, the second lens 5, the second diaphragm 6, sample stage 7 and is fixed its runing rest, baffle plate 8 and ccd image sensor 9 along light beam working direction coaxial row successively, and can realize about ccd image sensor, upper and lower, the stepping frame that moves forward and backward, with the computing machine 10 that is connected ccd image sensor; Wherein said attenuator, first lens, the first diaphragm, the second lens, the second diaphragm are fixed on optical bench, described baffle plate is fixed on ccd image sensor, the ccd image sensor fixed placement two orthogonal can left and right, on the stepping frame that moves up and down, then be fixed on the stepping frame that can move forward and backward along optical path direction.
Further, in above-mentioned laser coherence diffraction microscopic imaging device: described laser instrument is the He-Ne laser instrument, and the output light wavelength is 0.543 micron, and the pixel count of CCD is 1300 * 1340, and pixel size is 22.5 microns; Described attenuator attenuation multiple is 100 times; Described first lens focal length 300mm, second focal length of lens is divided into 50mm, the focus of two lens overlaps, and two lens are in the ratio 6:1 compression light beam of focal length, and the first diaphragm and the second diaphragm are placed on respectively on the focus of first lens and the second lens for eliminating the parasitic light of light path; Described sample stage is placed on the waist spot place of laser, and the runing rest rotation angle range of sample stage is 0 degree～180 degree; Described baffle plate is positive square, and length of side 3mm is arranged on the path of laser direct projection ccd image sensor for blocking direct projection to the laser on CCD; The diffracted signal that described ccd image sensor is collected is transferred to computing machine.
Apply the method for above-mentioned laser coherence diffraction microscopic imaging device, step is:
The first step: as Fig. 1, dispose a set of laser coherence diffraction imaging device, described device is shown laser instrument 1, attenuator 2, first lens 3, the first diaphragm 4, the second lens 5, the second diaphragm 6, sample stage 7 and is fixed its runing rest, baffle plate 8 and ccd image sensor 9 along light beam working direction coaxial row successively, and can realize about ccd image sensor, upper and lower, the stepping frame that moves forward and backward, with the computing machine 10 that is connected ccd image sensor; Wherein said laser instrument is the He-Ne laser instrument, and the output light wavelength is 0.543 micron, and the pixel count of CCD is 1300 * 1340, and pixel size is 22.5 microns;
Second step: compression, focusing and purification light beam, method is as follows:
Select attenuator, attenuation multiple is 100 times, decay is from the laser of laser emitting, the focal length that the focal length of selecting first lens is 300mm and the second lens is 50mm, ratio 6:1 compression light beam in the focal length of two lens, the focus of two lens overlaps, and diaphragm 4 is placed on the focus place of first lens 3, and diaphragm 6 is placed on the focus place of the second lens 5; Attenuator and lens are all wiped clean with lens wiping paper.
The 3rd step: put into sample, gather diffracted signal, method is as follows:
Sample stage is placed on the waist spot place of laser, and sample is fixed on sample stage, and as Fig. 2, sample is the rectangle that microsphere silica gel is arranged in, and length is 91.4 microns, and wide is 89.36 microns; The sample that moves up and down, find diffracted signal with CCD, and ccd image sensor, apart from sample 5cm, is selected the centre position collection signal of diffracted signal, and the time shutter is 6 seconds, exposure frequency 1000 times; Then adjust two orthogonal stepping framves, the ccd image sensor vertical optical path is moved, mobile ccd image sensor, to the upper left corner, the lower left corner, the upper right corner and the lower right corner of diffracted signal, gathers respectively the high angle signal of these four positions; Then adjust the stepping frame of placing along light path, ccd image sensor moved away from sample along light path, mobile after apart from the distance of sample for being respectively 17cm and 32cm, according to identical time shutter and the more low-angle diffracted signal of exposure frequency collection;
The 4th step: diffracted signal all is saved in computing machine; The diffracted signal in the centre that ccd image sensor is collected at the 5cm place, the upper left corner, the lower left corner, the upper right corner and the lower right corner synthesizes individual diffraction image in computing machine, ccd image sensor is filled up to the diffracted signal that 5cm place baffle plate blocks along 17cm and the 32cm place diffracted signal collected after light path moves, as Fig. 3, rebuild object amplitude and phase image with iterative algorithm, as Fig. 4.
The 5th step: every a sample of 3 degree rotations, every rotation once, just repeats the step of signals collecting, amplitude and Phase Build Out in third and fourth step, obtains amplitude and the phase image of sample room every 3 degree;
The 6th step: the 3-D view that sample room is used to the method synthetic body of tomography every amplitude and the phase image of 3 degree.
Claims (2)
1. a laser coherence diffraction microscopic imaging device, it is characterized in that: described device is shown laser instrument (1), attenuator (2), first lens (3), the first diaphragm (4), the second lens (5), the second diaphragm (6), sample stage (7) and is fixed its runing rest, baffle plate (8) and ccd image sensor (9) along light beam working direction coaxial row successively, and can realize about ccd image sensor, upper and lower, the stepping frame that moves forward and backward, with the computing machine that is connected ccd image sensor (10); Wherein said attenuator, first lens, the first diaphragm, the second lens, the second diaphragm are fixed on optical bench, described baffle plate is fixed on ccd image sensor, the ccd image sensor fixed placement two orthogonal can left and right, on the stepping frame that moves up and down, and then be fixed on the stepping frame that can move forward and backward along optical path direction.
2. laser coherence diffraction microscopic imaging device as claimed in claim 1, it is characterized in that: described attenuator attenuation multiple is 10 times～1000 times; The focal length of described first lens and the second lens is respectively 50mm～3000mm, the focus of two lens overlaps, two lens are in the ratio compression light beam of focal length, and the first diaphragm and the second diaphragm are placed on respectively on the focus of first lens and the second lens for eliminating the parasitic light of light path; Described sample stage is placed on the waist spot place of laser, and the runing rest rotation angle range of sample stage is 0 degree～180 degree; Described baffle plate is positive square, and length of side 1mm～3mm is arranged on the path of laser direct projection ccd image sensor for blocking direct projection to the laser on CCD; The diffracted signal that described ccd image sensor is collected is transferred to computing machine.
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| CN 201220645458 CN202956532U (en) | 2012-11-30 | 2012-11-30 | Laser coherence diffraction micro-imaging device |
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| CN 201220645458 CN202956532U (en) | 2012-11-30 | 2012-11-30 | Laser coherence diffraction micro-imaging device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102981261A (en) * | 2012-11-30 | 2013-03-20 | 山东大学 | Laser coherence diffraction microscopic imaging device and application thereof |
| CN112859314A (en) * | 2020-12-31 | 2021-05-28 | 山东建筑大学 | Single-pixel scanning super-resolution phase imaging device and method |
-
2012
- 2012-11-30 CN CN 201220645458 patent/CN202956532U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102981261A (en) * | 2012-11-30 | 2013-03-20 | 山东大学 | Laser coherence diffraction microscopic imaging device and application thereof |
| CN102981261B (en) * | 2012-11-30 | 2015-03-11 | 山东大学 | Laser coherence diffraction microscopic imaging device and application thereof |
| CN112859314A (en) * | 2020-12-31 | 2021-05-28 | 山东建筑大学 | Single-pixel scanning super-resolution phase imaging device and method |
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| C14 | Grant of patent or utility model | ||
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Granted publication date: 20130529 Effective date of abandoning: 20150311 |
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| AV01 | Patent right actively abandoned |
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| RGAV | Abandon patent right to avoid regrant |